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Characterizing Tmx And Chemotactic Receptor Arrays Through Bacterial Two-Hybrid And Beta-Galactosidase Assays, Adam Keith Hubler
Characterizing Tmx And Chemotactic Receptor Arrays Through Bacterial Two-Hybrid And Beta-Galactosidase Assays, Adam Keith Hubler
Chancellor’s Honors Program Projects
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An Ethylene-Binding Protein, Azoetr1, In Azospirillum Brasilense Sp7, Tiffany Nicole Thoms
An Ethylene-Binding Protein, Azoetr1, In Azospirillum Brasilense Sp7, Tiffany Nicole Thoms
Masters Theses
Ethylene is an important phytohormone produced by higher plants where it plays numerous roles in growth and development and in mediating responses to many environmental factors. The genome sequence of the soil alphaproteobacterium A. brasilense suggests that it encodes a homolog of an ethylene-binding protein (AzoETR1), similar to that found in plants. AzoETR1 also possesses a conserved histidine kinase domain with the azoETR1 gene encoded upstream of a putative response regulator. Therefore, this ethylene binding protein may be part of a two-component signal transduction pathway. Recombinant expression of the ethylene-binding domain of AzoETR1 in Pichia pastoris followed by an in …