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Gene Probe Analysis Of Soil Microbial Populations Selected By Amendment With 2,4-Dichlorophenoxyacetic Acid, William Holben, B. M. Schroeter, V. G.M. Calabrese, R. H. Olsen, J. K. Kukor, V. O. Biederbeck, James M. Tiedje
Gene Probe Analysis Of Soil Microbial Populations Selected By Amendment With 2,4-Dichlorophenoxyacetic Acid, William Holben, B. M. Schroeter, V. G.M. Calabrese, R. H. Olsen, J. K. Kukor, V. O. Biederbeck, James M. Tiedje
Biological Sciences Faculty Publications
Soils with a history of 2,4-dichlorophenoxyacetic acid (2,4-D) treatment at field application rates and control soils with no prior exposure to 2,4-D were amended with 2,4-D in the laboratory. Before and during these treatments, the populations of 2,4-D-degrading bacteria were monitored by most-probable-number (MPN) enumeration and hybridization analyses, using probes for the tfd genes of plasmid pJP4, which encode enzymes for 2,4-D degradation. Data obtained by these alternate methods were compared. Several months after the most recent field application of 2,4-D (approximately 1 ppm), soils with a 42-year history of 2,4-D treatment did not have significantly higher numbers of 2,4-D-degrading …
Identification Of The Nd Δ And Σ States And The 1,3Φ←←X 3Σ− G Transition Of O2 By Resonant Multiphoton Ionization, Robert J. Yokelson, R. J. Lipert, W. A. Chupka
Identification Of The Nd Δ And Σ States And The 1,3Φ←←X 3Σ− G Transition Of O2 By Resonant Multiphoton Ionization, Robert J. Yokelson, R. J. Lipert, W. A. Chupka
Chemistry and Biochemistry Faculty Publications
Spectra of the 3dRydberg state region of O2 have been obtained by two‐photon resonant ionization of the ground electronic state. By varying the rotational distribution and radiation polarization, all observed bands were identified and attributed to excitation of Σ, Δ, and Φ states. Earlier assignments were corrected. The Δ and Φ assignments are complete while the Σ assignments are so far incomplete.
Identification Of The Ns And Nd Rydberg States Of O2 For N=3–5, Robert J. Yokelson, R. J. Lipert, W. A. Chupka
Identification Of The Ns And Nd Rydberg States Of O2 For N=3–5, Robert J. Yokelson, R. J. Lipert, W. A. Chupka
Chemistry and Biochemistry Faculty Publications
The 4s‐3d and 5s‐4dRydberg complexes of diatomic oxygen have been studied by (2+1) resonance‐enhanced multiphoton ionization of the X 3∑ g − ground state of O2. We have located and identified at least two vibrational levels of each of the following states: Three of four expected 4sσ Π states; all four expected 5sσ Π states; 18 of 22 expected 3d states (with only the states of the 3dσ orbital remaining unobserved); and 5 of the 10 predicted 4dπ states. State assignments …
Avian Forelimb Muscles And Nonsteady Flight: Can Birds Fly Without Using The Muscles In Their Wings?, Kenneth P. Dial
Avian Forelimb Muscles And Nonsteady Flight: Can Birds Fly Without Using The Muscles In Their Wings?, Kenneth P. Dial
Biological Sciences Faculty Publications
Intensity patterns of electromyographic (EMG) signals from selected muscles of the wing were studied during different modes of flight in trained Rock doves (Columba livia). Shoulder muscles exhibited a stereotypic pattern producing maximal EMG intensity during the deceleration phases of the upstroke and the downstroke, whereas the muscles of the brachium and antebrachium acted primarily as joint stabilizers during level flapping flight. During nonsteady flight (e.g. takeoff, landing, vertical ascending flight), the distal forelimb muscles exhibited maximal EMG intensity; their primary function appears to be associated with changing the camber and planform of the wing during rapid oscillation. During steady …
Multiple Phosphate Positions In The Catalytic Site Of Glycogen Phosphorylase, Stephen R. Sprang, Neil B. Madsen, Stephen G. Withers
Multiple Phosphate Positions In The Catalytic Site Of Glycogen Phosphorylase, Stephen R. Sprang, Neil B. Madsen, Stephen G. Withers
Biological Sciences Faculty Publications
The three‐dimensional structure of an R‐state conformer of glycogen phosphorylase containing the coenzyme‐substrate analog pyridoxal‐5′‐diphosphate at the catalytic site (PLPP‐GPb) has been refined by X‐ray crystallography to a resolution of 2.87 Å. The molecule comprises four subunits of phosphorylase related by approximate 222 symmetry. Whereas the quaternary structure of R‐state PLPP‐GPb is similar to that of phosphorylase crystallized in the presence of ammonium sulfate (Barford, D. & Johnson, L.N., 1989, Nature 340, 609–616), the tertiary structures differ in that the two domains of the PLPP‐GPb subunits are rotated apart by 5° relative to the T‐state conformation. Global differences among the …
Intermediates In The Constitutive And Regulated Secretory Pathways Released In Vitro From Semi-Intact Cells, Mark L. Grimes, Regis B. Kelly
Intermediates In The Constitutive And Regulated Secretory Pathways Released In Vitro From Semi-Intact Cells, Mark L. Grimes, Regis B. Kelly
Biological Sciences Faculty Publications
Regulated secretory cells have two pathways that transport secreted proteins from the Golgi complex to the cell surface. To identify carrier vesicles involved in regulated and constitutive secretion, PC12 pheochromocytoma cells were labeled with [35S]sulfate to identify markers for the two secretory pathways, then mechanically permeabilized and incubated in vitro. Small constitutive secretory vesicles, containing mostly sulfated proteoglycans, accumulated during an in vitro incubation with ATP. In the presence of GTP gamma S, the constitutive vesicles became significantly more dense, suggesting that a coated intermediate was stabilized. Larger immature regulated secretory granules, enriched in sulfated secretogranin II, also escaped from …
Evaluating Habitat Suitability Using Relative Abundance And Fledging Success Of Red-Naped Sapsuckers, Bret W. Tobalske
Evaluating Habitat Suitability Using Relative Abundance And Fledging Success Of Red-Naped Sapsuckers, Bret W. Tobalske
Biological Sciences Faculty Publications
No abstract provided.
The Structure Of Human Lymphotoxin (Tumor Necrosis Factor-Β) At 1.9-Å Resolution, Michael J. Eckt, Mark Ultsch, Ernst Rinderknecht, Abraham M. De Vos, Stephen R. Sprang
The Structure Of Human Lymphotoxin (Tumor Necrosis Factor-Β) At 1.9-Å Resolution, Michael J. Eckt, Mark Ultsch, Ernst Rinderknecht, Abraham M. De Vos, Stephen R. Sprang
Biological Sciences Faculty Publications
The three-dimensional structure of recombinant human lymphotoxin (residues 24-171 of the mature protein) has been determined by x-ray crystallography at 1.9-Å resolution (Rcryst = 0.215 for I > 3σ(I)). Phases were derived by molecular replacement using tumor necrosis factor (TNF-α) as a search model. Like TNF-α, lymphotoxin (LT) folds to form a "jellyroll" β-sheet sandwich. Three-fold related LT subunits form a trimer stabilized primarily by hydrophobic interactions. A cluster of 6 basic residues around the 3-fold axis may account for the acid lability of the trimer. Although the structural cores of TNF-α and LT are similar, insertions and …
The Latent Tendencies Of Pai-1, Stephen R. Sprang
The Latent Tendencies Of Pai-1, Stephen R. Sprang
Biological Sciences Faculty Publications
No abstract provided.