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Biochemical And Genetic Characterization Of Ppca, A Periplasmic C-Type Cytochrome In Geobacter Sulfurreducens, Jon R. Lloyd, Allison L Hodges Myerson, Ching Leang, Maddalena V. Coppi, Stacey Cuifo, Barbara A. Methѐ, Derek Lovley, Steven J. Sandler
Biochemical And Genetic Characterization Of Ppca, A Periplasmic C-Type Cytochrome In Geobacter Sulfurreducens, Jon R. Lloyd, Allison L Hodges Myerson, Ching Leang, Maddalena V. Coppi, Stacey Cuifo, Barbara A. Methѐ, Derek Lovley, Steven J. Sandler
Steven Sandler
A 9.6 kDa periplasmic c -type cytochrome, designated PpcA, was purified from the Fe(III)-reducing bacterium Geobacter sulfurreducens and characterized. The purified protein is basic (pI 9.5), contains three haems and has an N-terminal amino acid sequence closely related to those of the previously described trihaem c (7) cytochromes of Geobacter metallireducens and Desulfuromonas acetoxidans. The gene encoding PpcA was identified from the G. sulfurreducens genome using the N-terminal sequence, and encodes a protein of 71 amino acids (molecular mass 9.58 kDa) with 49% identity to the c (7) cytochrome of D. acetoxidans. In order to determine the physiological role of …