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Life Sciences

University of Massachusetts Amherst

Theses/Dissertations

2014

Listeria monocytogenes

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Influence Of Lipoteichoic Acid On Listeria Monocytogenes Biofilm Formation And Cellular Stress, Imelda T. Brooks Nov 2014

Influence Of Lipoteichoic Acid On Listeria Monocytogenes Biofilm Formation And Cellular Stress, Imelda T. Brooks

Doctoral Dissertations

Lipoteichoic acid (LTA) is an important polymer on the surface of Gram-positive organisms, including the foodborne, biofilm-forming pathogen Listeria monocytogenes, and is connected to the cell membrane through a glycolipid backbone. Previous results have shown the importance of LTA in cell viability and biofilm formation. The purpose of this research is to further investigate the influence of LTA in L. monocytogenes biofilm formation and how the bacterium responds to environmental stress caused by antimicrobial exposures, and a combination of low temperature and high osmolarity. We created in frame deletion mutants along the operon lmo-2555-lmo2554-lmo2553, which have been shown …


Susceptibility Of Escherichia Coli O157:H7, Salmonella Enterica Sp., And Listeria Monocytogenes To Antimicrobial Mixed Micelle Delivery Systems, Chanelle N. Adams Nov 2014

Susceptibility Of Escherichia Coli O157:H7, Salmonella Enterica Sp., And Listeria Monocytogenes To Antimicrobial Mixed Micelle Delivery Systems, Chanelle N. Adams

Doctoral Dissertations

Within the food industry, there is an ever increasing demand to improve the quality of food and ensure safety from pathogenic/spoilage microorganisms. Globalization of the food industry in the 1990’s, in conjunction with the worldwide shipment of food created a need for the extension of shelf-life and enhanced maintenance of food quality (12). In the United States alone, the Centers for Disease Control and Prevention report that each year, roughly 1 in 6 Americans (or 48 million people) gets sick, 128,000 are hospitalized, and 3,000 die of foodborne diseases; this correlates to approximately $77.7 billion in economic loss annually (17). …