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A High-Resolution Map Of Arabidopsis Recombinant Inbred Lines By Whole-Genome Array Hybridization, Samuel P. Hazen, T. S. Singer, Y. Fan, H. S. Chang, T. Zhu, S. P. Briggs

Samuel P Hazen

Recombinant populations were the basis for Mendel's first genetic experiments and continue to be key to the study of genes, heredity, and genetic variation today. Genotyping several hundred thousand loci in a single assay by hybridizing genomic DNA to oligonucleotide arrays provides a powerful technique to improve precision linkage mapping. The genotypes of two accessions of Arabidopsis were compared by using a 400,000 feature exon-specific oligonucleotide array. Around 16,000 single feature polymorphisms (SFPs) were detected in ~8,000 of the ~26,000 genes represented on the array. Allelic variation at these loci was measured in a recombinant inbred line population, which defined …