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Aptamer; Dna; Dna aptamers; In vitro selection; Ligand; Prostate specific antigen; Psa; Psa1; Psa2; Psa3; Selection; Selex; Systemic evolution of ligands by experimental enrichment
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In Vitro Selection Of Dna Aptamers Against Prostate Cancer Peptide Biomarkers, Elif Kuguoglu
In Vitro Selection Of Dna Aptamers Against Prostate Cancer Peptide Biomarkers, Elif Kuguoglu
HIM 1990-2015
This project is aimed toward finding DNA aptamers against prostate cancer peptide antigens. DNA aptamers can function to find and indicate the presence of certain molecules in a specimen. These aptamers will be obtained through the process of evolutionary selection, a specific process called SELEX which stands for Systemic Evolution of Ligands by Experimental Enrichment. By conducting several rounds of SELEX, a DNA aptamer will be selected to bind to a known peptide antigen. A biotinylated column will be utilized to stabilize a random library of DNA aptamers, and those peptides that bind to certain aptamers will cause a conformational …