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Primary Structure Of The Carbohydrate-Binding Modules In Various Cellulolytic, Thermophilic, Anaerobic, Ethanol-Producing Isolates, Melek Özkan, Gülay Özcengi̇z
Primary Structure Of The Carbohydrate-Binding Modules In Various Cellulolytic, Thermophilic, Anaerobic, Ethanol-Producing Isolates, Melek Özkan, Gülay Özcengi̇z
Turkish Journal of Biology
In the present study, the carbohydrate-binding module (CBM) coding sequences of the cellulosomes of 13 thermophilic, cellulolytic, anaerobic, ethanol-producing bacterial isolates having some variations in their growth and cellulose degradation capacities were amplified by PCR and then sequenced. The sequence analysis of the amplicons revealed that CBMs of 7 of the isolates including the isolate 7-9-1 with the highest capacity of cellulose degradation in solid medium have 100% identity in both nucleotide and amino acid sequences to CipB of Clostridium thermocellum in the compared regions. On the other hand, CBM of the isolate 7-1-2, also having a high cellulolytic activity, …