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A Process To Produce Lactose Phosphate From Dairy Byproducts And Used As An Alternative To Emulsifying Salts In Processed Cheese Food Manufacture, Khalid Ahmed Alsaleem
A Process To Produce Lactose Phosphate From Dairy Byproducts And Used As An Alternative To Emulsifying Salts In Processed Cheese Food Manufacture, Khalid Ahmed Alsaleem
Electronic Theses and Dissertations
Lactose is the primary carbohydrate in most mammals' milk, commonly known as milk sugar. Milk permeate is a by-product of whey protein manufacturing through membrane technologies. It is cost-effective, available, and an excellent source of lactose. Sugar phosphorylation is a technique used to alter sugar's characteristics. It has numerous applications for developing dairy and food products. Lactose-6-phosphate (LP) is an organic compound that switches the hydrogen on lactose by monophosphate that has the potential to function as emulsifying salts (ES). ES, such as disodium phosphate (DSP) and trisodium citrate, have a critical effect on the emulsification characteristics of casein by …
Response Of Soil Biochemical And Physical Properties To Long Term Prairie Cordgrass And Kura Clover Intercropping System, Vaishnavi Varikuti
Response Of Soil Biochemical And Physical Properties To Long Term Prairie Cordgrass And Kura Clover Intercropping System, Vaishnavi Varikuti
Electronic Theses and Dissertations
Prairie cordgrass (Spartina pectinata) (PCG) is a warm-season perennial grass that can be used as a biofuel feedstock and can be grown on marginal lands. Previous studies on intercropping of a perennial legume i.e., kura clover (Trifolium ambiguum) (KC) with PCG can improve soil biochemical properties, increase biomass production, mitigate greenhouse gas (GHG) emissions while reducing the chemical fertilizer requirement. However, there is a lack of evidence about the effects of PCG production on soil biochemical and physical properties during the cropping season and at deeper soil depths in reference to support plant growth, environmental implications and enhance the soil …
Identifying Adp-Ribosylation As The Biochemical Signal That Marks Dna-Protein Crosslinks For Sprtn-Dependent Proteolysis, Katelyn Hurley
Identifying Adp-Ribosylation As The Biochemical Signal That Marks Dna-Protein Crosslinks For Sprtn-Dependent Proteolysis, Katelyn Hurley
Electronic Theses and Dissertations
DNA-protein crosslinks (DPCs) form when proteins covalently attach to DNA. It is estimated that hundreds of DPCs form in our cells each day. Because these lesions are cytotoxic, mammalian cells have evolved multiple intricate repair systems to remove DPCs and restore genome integrity. One mechanism mammalian cells rely on for DPC repair is direct proteolysis by the metalloprotease, SPRTN. While it is known that SPRTN degrades a heterogenous array of DPCs, including both naturally occurring and chemically induced, how SPRTN selects DPCs amongst chromatin-associated proteins remains unknown. Our main objective is to determine how DPC repair is regulated. In Chapter …
Evaluating Fungicide Timing, Efficacy, And Sensitivity As Well As Candidate Resistance Genes Against Fungi Causing Phomopsis Stem Canker In Sunflower, Ruchika Kashyap
Evaluating Fungicide Timing, Efficacy, And Sensitivity As Well As Candidate Resistance Genes Against Fungi Causing Phomopsis Stem Canker In Sunflower, Ruchika Kashyap
Electronic Theses and Dissertations
Phomopsis stem canker is one of the major diseases of sunflower (Helianthus annuus L.) caused by complex of Diaporthe spp., in the U.S. This disease became of economic importance after 2010 epidemic in Minnesota, North Dakota, and South Dakota and since then the prevalence of this diseases has increased due to increased average precipitation in these major sunflower growing regions of the U.S. The two important disease management strategies involve the use of fungicides and partially resistant hybrids. However, over the years, researchers and growers have seen varied results on Phomopsis stem canker disease severity reduction and yield gains, when …
Genistein-Enriched Pig Gut Microbiota Library As A Potential Probiotic Consortium, Theresah Amponsah
Genistein-Enriched Pig Gut Microbiota Library As A Potential Probiotic Consortium, Theresah Amponsah
Electronic Theses and Dissertations
Plant-based diets contain numerous flavonoid compounds that produce antibacterial effects and gut health improvement. Genistein is among the most abundant isoflavones present in a plant-based diet and can be found in high amounts in soy products (up to 1g Kg-1). We describe here a robust method to identify genistein tolerant and metabolizing bacteria in swine gut microbiota and to screen the strains that have antibacterial and immunestimulatory properties. Such strains could be developed as non-antibiotic alternatives to prevent enteric infections in pigs and improve gut immunity. To this end, a mini bioreactorbased system was used to enrich genistein metabolizing bacteria …
Manufacture Of Ingredients For Use In Clean Label Process Cheese Products, Ahmed Hammam
Manufacture Of Ingredients For Use In Clean Label Process Cheese Products, Ahmed Hammam
Electronic Theses and Dissertations
Micellar casein concentrate (MCC) is a high protein ingredient that can be used in several applications, such as manufacture of acid curd and process cheese products (PCP). Acid curd is one of the casein (CN) products, which can be obtained by precipitating the CN at a pH of 4.6 (isoelectric point) using starter cultures or direct acids. Acid curd has low mineral and calcium content due to the solubility of colloidal calcium phosphate at the isoelectric point in the whey. Acid curd and MCC can be utilized in manufacture of clean label PCP formulations. PCP is a dairy food prepared …
Evaluating Avian Use Of Cover Crops In The Corn Belt, Megan Figura
Evaluating Avian Use Of Cover Crops In The Corn Belt, Megan Figura
Electronic Theses and Dissertations
The tallgrass prairie of North America has changed drastically since colonial settlement, with up to 99% of this region converted for agriculture and other land-uses. Concurrent with grassland conversion, grassland birds have experienced the most extreme, consistent, and widespread population declines of any avian guild. Agricultural lands in the U.S. Midwest were able to provide adequate habitat for several bird species until the 1950’s; however, altered and intensified management practices have degraded much of remaining suitable habitat and undermined ecosystem functions. Consequently, many grassland birds have been identified as Species of Greatest Conservation Need (SGCN) in many State Wildlife Action …
A Post-Pneumonia Epizootic Evaluation Of The Rapid City, South Dakota Bighorn Sheep Herd, Amanda N. Ensrud
A Post-Pneumonia Epizootic Evaluation Of The Rapid City, South Dakota Bighorn Sheep Herd, Amanda N. Ensrud
Electronic Theses and Dissertations
Pneumonia is a major factor affecting populations of free-ranging bighorn sheep (Ovis canadensis) across western North America. Pneumonia can occur in large-scale epizootics, during which greater than half of the population typically dies. After these epizootics, surviving ewes continue to conceive and bear lambs. However, lamb recruitment may remain low due to periodic or annual pneumonia outbreaks causing high lamb mortality rates, sometimes greater than 90%. Our study focused on the Rapid City, South Dakota bighorn sheep (BHS) herd that has recorded pneumonia-induced population decline since 2009. The first objective was to improve lamb health and survival by identifying and …
Pathogen Prevalence In Domestic Sheep In Western Nebraska: Implications For Bighorn Sheep Conservation And Coexistence On A Multi-Use Landscape, Kaytlin Bohr
Electronic Theses and Dissertations
Bighorn sheep (Ovis canadensis) and domestic sheep (Ovis aries) are members of the same genus and thus share multiple pathogens that can be spread between them. One specific respiratory pathogen of concern is Mycoplasma ovipneumoniae (M. ovipneumoniae), which has been linked to pneumonia epizootics in bighorn sheep that are often characterized by all age die offs upon initial exposure followed by years of low lamb recruitment. Domestic sheep have been identified as one carrier of M. ovipneumoniae with transmission between sheep species occurring when there is close contact on the landscape. To prevent this cross-species transmission, importance has been placed …
Impact Of Storage And Different Variety On Proximate Composition And Functionality Of Lentils, Hussain Al Naser
Impact Of Storage And Different Variety On Proximate Composition And Functionality Of Lentils, Hussain Al Naser
Electronic Theses and Dissertations
Lentil (Lens culinaris) is an edible pulse and is a nutritious legume. The nutrient value and the application of lentils in other food resources have been well documented. However, there exists a lack of studies that focus on the storage impact of pulses on lentil composition and functionality. The focus of most studies has been on the composition of lentils while the composition relative to and functionality of stored lentils has not been sufficiently evaluated. Therefore, the objective of this research was to determine the effect of storage conditions on the nutrient composition and the functional properties of lentils. Four …
Understanding The Impact Of Human Germline Single-Nucleotide Variants, Samuel Adjei
Understanding The Impact Of Human Germline Single-Nucleotide Variants, Samuel Adjei
Electronic Theses and Dissertations
No abstract provided.
Optimization Of Forage Use In Diets Fed To Backgrounding And Finishing Cattle In The Northern Great Plains, Thomas G. Hamilton
Optimization Of Forage Use In Diets Fed To Backgrounding And Finishing Cattle In The Northern Great Plains, Thomas G. Hamilton
Electronic Theses and Dissertations
The two studies in this thesis were conducted to: 1) investigate the impact of corn silage moisture content and kernel processing at harvest on growth performance, efficiency of dietary net energy utilization, and carcass traits in finishing steers when fed at 20% DM inclusion in diets containing modified distillers grains plus solubles; and 2) determine the influence of equal cumulative roughage inclusion in a single diet or twodiet system during a 210-d backgrounding-finishing period in pre-conditioned beef steers on growth performance responses, efficiency of dietary net energy (NE) utilization, and carcass traits. Experiment 1 was a 112-d finishing experiment conducted …
Study Of Root System Architectural Traits Of Oat And Response To Endophyte Inoculation And Drought Stress, Krishna Ghimire
Study Of Root System Architectural Traits Of Oat And Response To Endophyte Inoculation And Drought Stress, Krishna Ghimire
Electronic Theses and Dissertations
Oat is an important cereal crop grown worldwide. Oats have the potential to contribute to human health due to their unique nutritional attributes. Developing oat cultivars with efficient root systems able to extract heterogeneously distributed soil resources can help maintain yield under drought conditions and in nutrient poor soil. Various root traits determine the soil volume that is explored by the root system for resource acquisition. Knowledge about the genetic control of oat root traits and response to biotic and abiotic environmental factors is lacking. Identifying quantitative trait loci associated with root traits and understanding the response of roots to …
Factors Influencing Mortality Of Stocked Rainbow Trout In Black Hills Reservoirs, Charles A. Mordhorst
Factors Influencing Mortality Of Stocked Rainbow Trout In Black Hills Reservoirs, Charles A. Mordhorst
Electronic Theses and Dissertations
Because return to angler is an important outcome of put-and-take fisheries, understanding mortality of stocked Rainbow Trout is fundamental to managing these fisheries. Harvest rates of stocked Rainbow Trout Oncorhynchus mykiss in the Black Hills of South Dakota are believed to be below management objectives. Rainbow Trout not harvested by anglers are assumed to be lost to various sources of mortality, raising concerns about the cost of the Rainbow Trout stocking program relative to the benefit provided to anglers. (Simpson 2008). This study evaluated the factors influencing mortality of Rainbow Trout stocked into Black Hills reservoirs. We assessed the effects …
Decoding The Mystery Of Antibiotic Persistence, Tahmina Hossain
Decoding The Mystery Of Antibiotic Persistence, Tahmina Hossain
Electronic Theses and Dissertations
This work combines microbiology, molecular biology, Next-Generation Sequencing and system biology approaches to explore the mechanism of antibiotic persistence: a multi-drug tolerant, non-dividing, and metabolically altered state present in a subpopulation of cells due to phenotypic diversity rather than genetic variation (i.e. mutations). Persister can survive lethal antibiotic state and resuscitate after the treatment period is over. They are considered as the major contributing factor behind recurring infections. They also have a high mutation rate, which increases the chances of bacteria gaining antibiotic resistance. The formation of this phenotypic variant (persister) threatens the therapeutic effectiveness of antibiotics and understanding how …
Effect Of Essential Oils In The Control Of Fungal Diseases In Small Grains, Esneider Mahecha Bojaca
Effect Of Essential Oils In The Control Of Fungal Diseases In Small Grains, Esneider Mahecha Bojaca
Electronic Theses and Dissertations
Spring wheat (Triticum aestivum L.) and oats (Avena sativa L.) are important crops due to their high consumption in our daily diet worldwide. They are the main food of consumption per capita due to the high amino-acid content as well of vitamins. However, grain production is limited due to the negative impact of the diseases that cause significant yield loss. Diseases in oats, such as crown rust caused by Puccinia coronata f. sp. avenae (Pca), and in wheat such as Fusarium head blight (FHB) caused by the fungus Fusarium graminearum. Foliar diseases such as tan spot incited by the fungi …
Northern Great Plains Saline Sodic Soil Development, Classification, Remediation, And Management., Shaina Westhoff
Northern Great Plains Saline Sodic Soil Development, Classification, Remediation, And Management., Shaina Westhoff
Electronic Theses and Dissertations
Soil salinity and sodicity are issues of growing concern in the United States (U.S.) and globally. Knowledge gaps for glaciated, dryland salt-affected soils exist because much original salinity and sodicity research focused on irrigated systems. Land managers are being asked to produce food, feed, fiber, and fuel for an expanding global population. The number of land managers and crop advisors who are affected by these soils is increasing. Addressing salinity and sodicity knowledge gaps will be critical for their success. Salinity and sodicity have been impeding crop productivity since the advent of cultivation. Saline and sodic soils form via multiple …
Development Of Genomic Resources In Vitis Riparia For Discoveries On Pre- And Post-Transcriptional Molecular Regulators Of Early Induction Into Endodormancy, Michael Robben
Electronic Theses and Dissertations
Grapevine is one of the most important fruit crops in the world, responsible for billions in global sales annually. The largest threat to grapevine and other crop production is global climate change resulting human activities. This brings unpredictable and drastic changes in ambient air temperatures to many climates in which grapes are grown. Lower temperatures and inclement weather are already responsible for millions in lost revenue due to tissue damage of established plants. Thus, protecting grapevine crops from weather-related damage is the biggest concern to growers aside from pathogen- and diseaserelated crop damage. The primary mechanism for winter survival in …
E. Coli Persister Cell Survival And Rhizobia Attachment To Soybean Roots, Tanim Islam
E. Coli Persister Cell Survival And Rhizobia Attachment To Soybean Roots, Tanim Islam
Electronic Theses and Dissertations
The theme of this thesis revolves around how bacteria respond and thrive during stress. Chapters 1-3 are about how bacteria deal with life-threatening antibiotics. Chapter 4 covers new research on how bacteria can move from a stressful individual lifestyle (free-living bacteria) to initiating a symbiotic relationship with a plant (a less stressful lifestyle). In Chapter 1, I briefly summarize the current state of knowledge in the field of antibiotic resistance and persistence. In Chapter 2, I add to this knowledge by providing new insights into several antibiotics' potency and exploring the antibiotic Eagle effect. In Chapter 3, I use pyruvate …
Microbial Communities And Nitrogen Dynamics In Prairie And Cropland Soils, Bikram Kumar Das
Microbial Communities And Nitrogen Dynamics In Prairie And Cropland Soils, Bikram Kumar Das
Electronic Theses and Dissertations
Soil microbial communities are affected by many factors, such as soil nitrogen (N) and the quantity of grass-root exudates, changing seasons, fertilization method, plant diversity, and their origin. This holds for both natural and fertilized soils. This research was intended to expand the current understanding of soil microbial interactions associated with ecological functions. The objectives of the research were (I) to characterize prokaryotic soil communities, estimate functional potential, and quantify nitrogen cycle-associated prokaryotic gene expressions across three phases of the growing season, (II) to explore the diazotrophic community composition in a natural system using nifH sequencing as well as 16S …
Role Of Host Restriction Factors On Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Replication, Pratik Katwal
Role Of Host Restriction Factors On Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Replication, Pratik Katwal
Electronic Theses and Dissertations
In this study, the role of IFITM3 on PRRSV replication was studied in vitro by expressing exogenous IFITM3 in MARC-145 cells. An average of 31% reduction in PRRSV N protein expression and an average of 5.4 fold decrease in virus titer in the supernatant were observed in IFITM3 overexpressing cells as compared to vector control cells at 24 hours post infection (hpi). Moreover, there was a positive correlation between interferon- induced IFITM3 up-regulation and reduced PRRSV replication. To determine the role of endogenous IFITM3 in PRRSV replication, siRNA induced knockdown of IFITM3 was employed. RT-PCR validated the successful silencing of …
It's Not Just No-Till: Crop Rotations Are Key To Improving Soil Quality And Grain Yields At Dakota Lakes Research Farm, Natalie Sturm
It's Not Just No-Till: Crop Rotations Are Key To Improving Soil Quality And Grain Yields At Dakota Lakes Research Farm, Natalie Sturm
Electronic Theses and Dissertations
No-till is increasingly promoted as a vital agricultural practice to address the challenge of improving soil quality while maintaining the ability to produce food, feed, fuel, and fiber. However, questions remain regarding 1) how different crop rotations impact soil quality under no-till management specifically and 2) whether significant improvements in soil quality can be made without negatively impacted crop yields. In this study, we demonstrate that even under the same long-term no-till management, differences in grain yields and soil biological, chemical, and physical properties exist between crop rotations at Dakota Lakes Research Farm in central South Dakota. We propose that …
Regenerative Agriculture Effects On Invertebrate And Bird Communities And Insect-Provided Ecosystem Services, Alex Michels
Regenerative Agriculture Effects On Invertebrate And Bird Communities And Insect-Provided Ecosystem Services, Alex Michels
Electronic Theses and Dissertations
Conventional agricultural practices can have unintended consequences on the environment and wildlife. Insects and birds are declining at rapid rates around the world, and the current conventional agricultural paradigm is a major driver through habitat loss and the intensification of production. Invertebrates in agroecosystems provide services to both farmers and the rest of society. Regenerative systems may promote the functioning of an agroecosystem by influencing invertebrate abundance, diversity, and ecosystem services and mitigate bird and insect declines through conservation practices that increase soil health, reduce disturbances, and increase biological diversity. Here I address knowledge gaps of the effects of regenerative …
Identifying Sources Of Resistance And Fungicide Efficacy Against Fungi Causing Phomopsis Stem Canker In Sunflower, Renan Guidini
Identifying Sources Of Resistance And Fungicide Efficacy Against Fungi Causing Phomopsis Stem Canker In Sunflower, Renan Guidini
Electronic Theses and Dissertations
Phomopsis stem canker is a major threat to sunflower (Helianthus annuus L.) production worldwide and its prevalence has increased from 1.5% in 2001 to 61.0% in 2015 in the sunflower producing U.S. states of Colorado, Kansas, Minnesota, Nebraska, North Dakota, South Dakota, and Texas. The disease causes early senescence and lodging of sunflower plants resulting in yield losses up to 40% and oil content up to 25%. Several species of Diaporthe have been reported to cause Phomopsis stem canker. In the United States, Diaporthe helianthi Munyt.-Cvetk. et al. and D. gulyae Shivas et al., have been reported as predominant causal …
Phomopsis Stem Canker Of Sunflower (Helianthus Annuus L.): Etiology And Epidemiological Conditions Affecting Endophytic Population Of Diaporthe Spp., And Fungicide Efficacy, Nabin Kumar Dangal
Phomopsis Stem Canker Of Sunflower (Helianthus Annuus L.): Etiology And Epidemiological Conditions Affecting Endophytic Population Of Diaporthe Spp., And Fungicide Efficacy, Nabin Kumar Dangal
Electronic Theses and Dissertations
Phomopsis stem canker (PSC), caused by species of Diaporthe, is a major disease of sunflower (Helianthus annuus L.) in the U.S., which can cause yield loss over 40%. The disease prevalence increased since 2010 in the major sunflower producing U.S. states of Nebraska, Minnesota, North Dakota, and South Dakota. Previous studies on the use of foliar fungicides in managing Phomopsis stem canker indicated mixed results on their efficacy in disease reduction and yield gain. Additionally, we do not have sufficient information on asymptomatic colonization by endophytic Diaporthe on sunflower tissue and how foliar fungicide and weather parameters affect the prevalence …
Leveraging Queueing Theory To Develop Advanced Synthetic Biological Circuits, Prajakta K. Jadhav
Leveraging Queueing Theory To Develop Advanced Synthetic Biological Circuits, Prajakta K. Jadhav
Electronic Theses and Dissertations
The complexity and redundancy in the network topology of the cell hinder our understanding of the natural system, making it challenging to engineer living organisms with the desired functionality. The long-term goal of synthetic biology and bioengineering is to engineer cells to perform specific functions with increased robustness. The robust engineered systems can be easily applied to different species with the same outcome. An effective approach is a bottom-up approach where we tease apart the biological pathways and study them independently. Understanding the underlying mechanisms of biological pathways can be further applied to construct complex biological circuits that function similarly …
Soil Hydro-Physical Properties, Computed Tomography Measured Pore Parameters, And Soil Health Indicators As Influenced By Tillage And Crop Rotation Systems, Goutham Thotakuri
Soil Hydro-Physical Properties, Computed Tomography Measured Pore Parameters, And Soil Health Indicators As Influenced By Tillage And Crop Rotation Systems, Goutham Thotakuri
Electronic Theses and Dissertations
Long-term tillage and crop rotation systems are important agricultural management practices as these can have direct impact on the soil’s key properties. The objectives of this study were to (ⅰ) quantify the soil pore characteristics under long-term tillage and crop rotation using X-ray computed tomography (XCT) and to assess the relationships between XCT-measured pore parameters and soil hydro-physical properties; and (ⅱ) evaluate the impacts of long-term tillage and crop rotation on select soil health indicators. The objective (ⅰ) was carried out at Haskell Agricultural Laboratory (HAL), Concord, NE; and objective (ⅰi) was carried out at South Central Agricultural Laboratory (SCAL), …
Impact Of Storage Conditions And Varieties On The Composition, Physical Properties, And Functionality Of Dry Peas, Sushmita Karki
Impact Of Storage Conditions And Varieties On The Composition, Physical Properties, And Functionality Of Dry Peas, Sushmita Karki
Electronic Theses and Dissertations
Dry peas are sustainable, healthy, and nutritious pulse crop and are excellent source of protein and complex carbohydrates as well as micronutrients. The effects of storage conditions on composition of beans and cereals have been widely reported. The information gained contributed significantly to better storage practices for these commodities. However, literature addressing the chemical composition, functionality, and physical characteristics of dry pea seeds upon storage is scarce. This study was designed to address the impact of storage conditions and varieties on the nutrient profile, functionality, and physical properties of dry pea seeds. Six different varieties of dry peas were stored …
Managing Water Quantity And Quality With Subsurface Drainage In Eastern South Dakota, Morghan Hurst
Managing Water Quantity And Quality With Subsurface Drainage In Eastern South Dakota, Morghan Hurst
Electronic Theses and Dissertations
Moisture extremes (excess and deficit) impact crop loss and water quality. Due to excessively wet springs and dry summers, crop damage can occur within the same county or field within the same year. To determine the magnitude of this problem in eastern South Dakota, indemnified crop insurance data for drought and excessive moisture claims were assessed for the years 1991-2020 for the occurrence of both excess moisture and drought in four counties in eastern South Dakota. Results show that there is greater than a 70% chance of the moisture extremes happening in the same year, making subsurface drainage, controlled drainage, …
Production Of Calcium Reduced Micellar Casein Concentrate Powders And Their Use In Process Cheese Products, Anil Kommineni
Production Of Calcium Reduced Micellar Casein Concentrate Powders And Their Use In Process Cheese Products, Anil Kommineni
Electronic Theses and Dissertations
Protein is an essential dietary component, and sufficient intake is vital in a healthy and balanced diet. Consumers are becoming increasingly aware of and knowledgeable about the role of protein in the diet. Two of the next-generation dairy protein ingredients isolated from milk are micellar casein (MCC) and milk-derived whey protein, isolated from skim milk using microfiltration (MF). Membrane filtration has been used extensively by the dairy industry to produce a variety of dairy ingredients from milk. MCC manufactured from freshly pasteurized milk can be directly consumed or as a supplement to fortify and enhance nutritional qualities in processed food …