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Treatment Of Nerve Impulse Data For Comparison With Theory, Bruce Knight, Scott E. Brodie, Lawrence Sirovich
Treatment Of Nerve Impulse Data For Comparison With Theory, Bruce Knight, Scott E. Brodie, Lawrence Sirovich
Knight Laboratory
A procedure is given for the comparison of nerve impulse data with model predictions. This method utilizes information in the nerve impulse train that is ignored by the post-stimulus-onset histogram and thereby gives an improved signal-to-noise ratio. Comparison of observed responses in the Limulus retina with predictions derived from a detailed model gives good agreement.
Nonlinear Analysis With An Arbitrary Stimulus Ensemble, Jonathan D. Victor, Bruce Knight
Nonlinear Analysis With An Arbitrary Stimulus Ensemble, Jonathan D. Victor, Bruce Knight
Knight Laboratory
No abstract provided.
Probing Mitotic Chromosome Structure And Arrangement Using Micromanipulation, Bruce R. Korf
Probing Mitotic Chromosome Structure And Arrangement Using Micromanipulation, Bruce R. Korf
Student Theses and Dissertations
The Indian deer, (Muntiacus muntjak), has the lowest known diploid chromosome number (seven in the male) for a mammal. Mitotic chromosomes can be removed from cells either by extraction with microneedles or by inducing chromosome expulsion with a drop of silicone oil applied to the cell. The entire diploid complement is removed by virtue of interconnecting fibers among the chromosomes. The chromosomes are brought to the surface of the cover slip for analysis, where each can be identified morphologically with phase contrast optics. The chromosomes are sticky and extensible, and parts of chromosomes can be dissected by cutting with microneedles. …
Early Events In The Biosynthesis And Subcellular Consignment Of Secretory And Membrane Proteins, Vishwanath R. Lingappa
Early Events In The Biosynthesis And Subcellular Consignment Of Secretory And Membrane Proteins, Vishwanath R. Lingappa
Student Theses and Dissertations
The biosynthetic mechanisms responsible for the segregation of secreted proteins and for the insertion of integral membrane proteins have been examined in light of the signal hypothesis. Model systems for the study of the biosynthesis of these different classes of proteins, as well as for the study of specific events common to both, have been developed. Their common as well as distinctive features have been probed by a variety of different experimental approaches involving cell free protein synthesizing systems supplemented with heterologous microsomal membranes either during or after translation: a) proteolytic enzymes, SDS-PAGE and autoradiography have been used to characterize …
Sensory And Hypothalamic Control Of Lordosis Behavior In Female Rodents, Kirk Randall Manogue
Sensory And Hypothalamic Control Of Lordosis Behavior In Female Rodents, Kirk Randall Manogue
Student Theses and Dissertations
When applied during the sustained lordosis response of female hamsters, gentle probing at points on the perineal surface elicited stereotyped rump and tail movements. The rump movements always served to rapidly center the vaginal opening beneath the continuing stimulation. Tail deflections were only elicited by stimulation applied above and lateral to the vaginal opening. The tail was deflected away from the side stimulated. These observations help to complete the description of the stimulus-response relations of the component reflexes which comprise the complete lordosis response in hamsters. The movements would serve to facilitate vaginal access during copulation and foster accurate targeting …
Studies On Chloroplast Protein Synthesis In Vivo And In Vitro, Karl Stanley Matlin
Studies On Chloroplast Protein Synthesis In Vivo And In Vitro, Karl Stanley Matlin
Student Theses and Dissertations
Protein synthesis in the Chlamydomonas reinhardtii chloroplast was investigated by in vivo and in vitro approaches. The site of synthesis of chloroplast proteins was examined to determine how many proteins were synthesized inside the chloroplast. Cells were labeled in vivo with [35S]-sulfate in the presence of either. chloroplast or cytoplasmic protein synthesis inhibitors, and fractionated into soluble proteins, thylakoid membranes, and chloroplast ribosomal subunits. The use of high specific activity sulfate and improved gel electrophoretic techniques were key features of this study because they permitted minor chloroplast products to be detected for the first time. It was determined …
Analysis And Synthesis Of The Dynamic Response Of Retinal Neurons, Scott E. Brodie
Analysis And Synthesis Of The Dynamic Response Of Retinal Neurons, Scott E. Brodie
Student Theses and Dissertations
theory of linear systems analysis is developed in a form directly applicable to the treatment of the Limulus retina. The dynamics of the retina may conveniently be characterized by means of a spatiotemporal transfer function, which summarizes the response of the system to moving sinusoidal gratings ("analysis"). The response of the retina to an arbitrary stimulus may then be calculated by addition of the response to suitably weighted sinusoidal stimuli ("synthesis"). Responses were obtained from the in-situ retina by means of extracellular recording of impulse activity in single optic nerve fibers. Test ommatidia were chosen in the interior of the …