Digital Commons Network^™

The Effect Of Jogging On Anterior Knee Laxity After Anterior Cruciate Ligament Reconstruction, Fawzi F. Bouzubar

Rehabilitation Sciences Theses & Dissertations

Jogging is commonly performed in the latter stages of the rehabilitation program (2½-3 months) following ACL reconstruction. However, its cyclic loading effect on the ACL graft, 4-6 months after surgery has not been examined. Therefore, the purpose of this study was to examine changes in anterior knee laxity before and after 15 minutes of jogging on the treadmill. Subjects (N=l 1) were 8 males and 3 females (X age= 30.27 ± 4.76 years; heights= 175.7 ± 10.7 cm; weights= 81.1 ± 15.9 kg) with unilateral ACL reconstruction (central 1/3 patellar tendon graft, postoperation range = 4-6 months). Measurements were made …

Changes In Small Mammal Community Attributes Associated With Increasing Pine Stand Age In Managed Pine Plantations In Southeastern Virginia, James Douglas Dolan

Biological Sciences Theses & Dissertations

Loblolly pine plantations were examined at different ages to identify small mammal community attributes in relation to the succession of the plant community. Forest floor and understory plant communities were characterized. Small mammals were collected by Fitch (live) traps and pitfall traps in four age classes during five seasons of study. Fitch live traps and pitfall traps were used in conjunction with one another to obtain the most accurate depiction of the small mammal community. Fitch traps accounted for 65 % of small mammal captures and 7 of 9 species captured. Small mammal abundance and biomass declined with increasing stand …

Patellofemoral Joint Compressive Forces During Backward And Forward Running At The Same Speed, Hesham N. Alrowayeh

Rehabilitation Sciences Theses & Dissertations

A previous study found that the patellofemoral joint compressive force (PFJRF) during backward running was less than during forward running at free speed. Therefore, the purpose of this study was to compare the patellofemoral joint compressive forces during backward and forward running at the same speed. Ten runners (4 females, 6 males) between the age of 20-25 (X=22, SD=2) ran in backward and forward directions at the slow speed of 2.3 mph (1.0 m.sec^-1). Using the inverse dynamic technique the knee extensor moment and PFJRF during the slow backward and forward running were calculated. Results showed that the …

The Effect Of Floor Sitting Posture On Low-Back Pain, Knee Pain And Hip Pain Among Kuwaiti Men, Sameera H. Al-Jedi

Rehabilitation Sciences Theses & Dissertations

Background and Purpose. Ground resting posture is common in Kuwait and other Arab communities. Kuwaiti men gather daily, especially on weekend nights socializing in the Diwaniya. Diwaniya is a special room in every Kuwaiti house. The Diwaniya is traditionally furnished with seating cushions and pillows. Men are seated on the floor in a tailor-sitting posture for long periods of time. The tailor-sitting posture flexes the trunk. hips, and knees. The flexion position has been shown to decrease the lumbar lordosis and increase joint compression in hips and knees. However there are no studies about the effect of floor sitting …

Comparison Of The Emg Activity Of The Supraspinatus And Infraspinatus Muscles During Various Closed Chain Exercises, I-Chen Lin

Rehabilitation Sciences Theses & Dissertations

Advantages of closed chain exercises used in the lower extremity have been well documented. However, the effects of closed chain exercises on the upper extremities have not been studied very much. Thus, the purpose of this research is to analyze the EMG activity of the supraspinatus and infraspinatus muscles and compare the relative amounts of activities by performing different isotonic closed chain exercises and an open chain exercise in normal subjects. The supraspinatus and infraspinatus muscles in 1 o healthy subjects were studied with fine wire, intramuscular, electromyographic electrodes while performing 5 closed chain exercises and a D2. open chain …

Mouse Embryo Development In The Presence Of Capsaicin, Carlos Santiago Villar-Gosalvez

Theses and Dissertations in Biomedical Sciences

Capsaicin is the pungent agent found in hot peppers of the Capsicum genus. It is a potent neurotoxin that stimulates the degranulation and degeneration of C-afferent neurons. Capsaicin is widely used as a food condiment and medicine. Human exposure of capsaicin can exceed levels shown to be neurotoxic in laboratory animals. Additionally, capsaicin can cross the blood/placenta barrier and affect an embryo in utero. In order to assay the potential for toxicity to human embryos, mouse embryos were exposed to capsaicin and the effect of the capsaicin on embryo development was measured. Embryos were co-cultured in Krebs medium with 1% …

Development Of Fluorescent Techniques In The Kinetic Characterization Of Reverse Transcriptase, Kevin Kevin Howerton

Chemistry & Biochemistry Theses & Dissertations

Reverse transcriptase (RT) is an RNA-directed DNA polymerase isolated from human immunodeficiency virus (HIV) and other retroviruses. It has been the primary target for anti-HIV research since the discovery of its vital role in the retroviral life-cycle. The conventional method for carrying out kinetic characterization and inhibition studies of reverse transcriptase involves the use of radioactive materials. Although this method is effective, it is both time consuming, due to a necessary repeated washing step, and hazardous to the worker and environment. Furthermore, strict laws limit the use of radioactive materials to licensed workers and laboratories. In response to these concerns, …

Expression, Isolation And Purification Of Human Zona Pellucida Protein 3, Ting-Fung Chi

Theses and Dissertations in Biomedical Sciences

Mammalian fertilization involves interactions of sperm surface receptors with the ligands of the zona pellucida, an extracellular matrix surrounding the ovulated oocytes. In humans, the zona pellucida is composed of three major glycoproteins. One of them, ZP3, participates in the primary sperm binding and in the subsequent triggering of the spermatozoa's acrosome reaction. Studies on the role of this specific protein in the human fertilization process are hampered by the limited amount of available biologically functional proteins.

By use of a pcDNA 3.1(+) expression vector, a transfecting-vector was constructed containing a 1.3 kb histidine tagged hZP3 cDNA. This histidine tagged …

Characterization Of The Biological Functions Of Human Recombinant Zona Pellucida Protein 3, Yu Wen Juan

Theses and Dissertations in Biomedical Sciences

Recombinant human zona pellucida protein 3 (rhZP3), expressed. isolated and purified from PA-1 cells, is characterized for its biological activity and the role in the signal transduction pathway. Characterization of the biological activity of rhZP3 was detected by hemizona assay and immunofluorescence staining of acrosome reaction The results indicated that rhZP3 exhibited an inhibition in the binding assay (HZI 43.6 +/-3.3; n = 9; 30 ng/mL rhZP3) and induction of acrosome reaction (198.6% +/- 77.2% increase from baseline; n = 29; 30 ng/mL rhZP3). It was further confirmed by the transmission electron microscopy that there was no difference in morphology …

Leukemia Inhibitory Factor (Lif): Murine Preimplantation Embryo Development, Implantation Rates, And Skeletal Development, Michael Hayes Mitchell

Theses and Dissertations in Biomedical Sciences

Leukemia inhibitory factor (LIF) is a pleiotropic cytokine which demonstrates perplexing physiological effects. It has been demonstrated that LIF is essential for implantation in mice. Little is known relating to the manner by which LIF effects pre-implantation and post-implantation development. The objectives of this project were to determine the effects LIF on pre-implantation development, to determine the effects that it may have on implantation rates, successful pregnancy rates, and resorption rates, and to determine the effects that LIF has on the skeletal development of mice. For the embryo transfer experiments, embryos were exposed to test compounds in the transfer medium …

Efficacy Of AllowashTm Solution In Solubilization Of Bone Marrow, Alyce Marie Linthurst

Biological Sciences Theses & Dissertations

LifeNet currently cleans allograft bone utilizing Allowash^TM Technology, which encompasses the use of a closed continuous circulation chamber to remove bone marrow by flushing it with Allowash^TM Solution for a prescribed time and temperature after which, it is rinsed with various solutions to remove residual detergent that could be toxic to the recipient. A portion of this process employs Allowash^TM Solution to improve the solubilization and removal of protein and cellular elements in the red bone marrow. This study examines the effectiveness of Allowash^TM Solution in comparison to its component detergents Nonidet P40, Nonoxynol 9 and …

Developmental And Hormonal Regulation Of The Expression Of Cytosolic Phospholipase A2 Protein Levels In Baboon Placenta, Kathleen Elizabeth Dunstan

Chemistry & Biochemistry Theses & Dissertations

Cytosolic phospholipase A₂ ( cPLA₂) specifically catalyzes the release of arachidonate from membrane phospholipids, thus initiating synthesis of prostaglandins, thromboxanes, and other eicosanoids. The human and nonhuman primate placenta actively synthesize high levels of eicosanoids which play both an autocrine and paracrine role in placental function. Previous studies in Dr. Miriam D. Rosenthal's laboratory have shown that cPLA₂ mRNA levels are developmentally regulated. The present study was designed to determine whether placental cPLA₂ protein levels increased during gestation. Since the marked increases in estrogen levels during normal pregnancy have been shown to enhance morphological and …

Modeling Environmental Effects On Msx Prevalence And Intensity In Eastern Oyster (Crassostrea Virginica) Populations, Michelle Christine Paraso

OES Theses and Dissertations

An oyster population model coupled with a model for Haplosporidium nelsoni, the causative agent of the oyster disease MSX, was used with salinity time-series constructed from Delaware River flow measurements to study environmentally-induced variations in the annual cycle of this disease. Simulations with this model were designed to investigate the effect of increased or decreased spring freshwater discharge, the timing of high freshwater runoff, the presence or absence of a fall or late spring phytoplankton bloom, and the occurrence of a warm winter on MSX prevalence and intensity in Delaware Bay oyster populations. Model simulations for the lower Bay site …

Pressure-Induced Intracellular Signaling In Isolated Arteries, Victor A. Miriel

Theses and Dissertations in Biomedical Sciences

The ability of cells to respond to mechanical stimuli has been studied through a variety of techniques in numerous cell types. The cells of the vascular wall have adapted to specific mechanical stresses through the activation of intracellular signaling pathways which result in cell-specific responses such as hypertrophy, hyperplasia, proliferation, and migration. Vascular smooth muscle of the arteries have been shown to be sensitive to mechanical stimuli such as stretch, and pressure.

This study attempts to add to the current knowledge of mechanotransduction by utilizing the isolated artery preparation. This preparation allows for the study of vascular smooth muscle signal …

The Role Of Glycoconjugates In Mediating Human Fertilization And Induction Of Fetomaternal Tolerance, Manish S. Patankar

Theses and Dissertations in Biomedical Sciences

Using the hemizona assay (HZA), a in vitro sperm-egg binding assay, we show that specific glycoconjugates known to inhibit immune cell interactions mediated by the selectins, potently block human sperm-egg binding. The selectin ligand sialyl Lewis^x inhibits sperm binding in the HZA by 60% at a concentration of 1 mg/ml. Our data indicates that glycodelin-A, a endometrial glycoprotein known to block sperm-egg binding in the HZA at low concentrations expresses unusual fucosylated lacdiNAc type glycans. The fucosylated lacdiNAc type sugars have been previously shown to be 15-20 fold more potent ligands of E-selectin. Glycodelin-S a seminal plasma glycoform of …

Effect Of Sampling Errors On Estimates Of Recruitment And Fishing Mortality From Separable Virtual Population Analysis, Emmanis Dorval

Biological Sciences Theses & Dissertations

Separable virtual population analysis (SVPA) models provide estimates of historical recruitment and fishing mortality from analyzing catch data based on the separability of fishing mortality into age specific-gear selection and yearly mortality. These models assume that the catch is randomly sampled and that sampling error is a random variable with constant variance and mean equal to zero. These models do not account for sampling measurement errors that occur when the catch partitioned by age is not measured on every sampling unit without error, and spatio-temporal errors that occur when the observed catch is not representative of the harvested population throughout …

Reaction-Diffusion Models Of Cancer Dispersion, Kim Yvette Ward

Mathematics & Statistics Theses & Dissertations

The phenomenological modeling of the spatial distribution and temporal evolution of one-dimensional models of cancer dispersion are studied. The models discussed pertain primarily to the transition of a tumor from an initial neoplasm to the dormant avascular state, i.e. just prior to the vascular state, whenever that may occur. Initiating the study is the mathematical analysis of a reaction-diffusion model describing the interaction between cancer cells, normal cells and growth inhibitor. The model leads to several predictions, some of which are supported by experimental data and clinical observations $\lbrack25\rbrack$. We will examine the effects of additional terms on these characteristics. …

Studies On The Biology Of Striga Aspera (Scrophulariaceae) In Nigeria, Emmanuel Izaka Aigbokhan

Biological Sciences Theses & Dissertations

Studies on Striga aspera are few and little information exists on its relationship with the morphologically similar and much studied S. hermonthica. Both species are sympatric in West Africa and if they hybridize, may serve as virulent gene reservoirs for each other.

The primary focus of this study was on the biology of S. aspera in Nigeria. The research focussed on its distribution, phenology, cytology, hybridization, seed dormancy, viability and germination in relation to S. hermonthica. Related studies involving the hybrids include genetic and morphological variability, pathogenicity and the simultaneous dual infection of a maize plant by both species.

In …

Ecology Of The Eastern Cottonmouth (Agkistrodon P. Piscivorus) At Back Bay National Wildlife Refuge: A Comparative Study Of Natural And Anthropogenic Marsh Habitats, Chad Lee Cross

Biological Sciences Theses & Dissertations

Mark-recapture sampling and radiotelemetry were used to investigate populations of the eastern cottonmouth, Agkistrodon p. piscivorus, in both natural and anthropogenic marsh habitats at Back Bay National Wildlife Refuge (BBNWR), Virginia Beach, Virginia from autumn 1995 to late spring 1998. Mark-recapture subjects were captured, marked by ventral scale-clipping, and released back into the population. A modified Schnabel Census estimator was used to estimate population sizes and corresponding densities in both marsh systems based on a total of 244 captures of 222 individuals. Most snakes were found >0.05 in from water, but it was apparent that proximity to water played …

Non-Volatile Dissolved Organic Iodine In Marine Water, Xianhao Cheng

OES Theses and Dissertations

An analytical scheme for the determination of marine DOI has been established. The concentration of DOI is estimated as total iodine (TI) minus total inorganic iodine (TII). The concentration of total iodine is determined as [special characters omitted] after DOI has been oxidized to inorganic iodine quantitatively by intensive UV-irradiation and all the inorganic iodine in the samples had been converted to [special characters omitted] by the addition of NaClO.

Production of DOI in seawater can be via both non-phytoplankton and phytoplankton related processes. In the former, iodide is converted to DOI. In the latter, most likely, iodate is converted …