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Effect Of The Chesapeake-Elizabeth Sewage Outfall Virginia Beach, Virginia On The Distribution And Ecology Of Benthic Foraminifera, James M. Bates Jr.

OES Theses and Dissertations

Sediment and bottom water samples from seven stations at the Chesapeake Elizabeth sewage outfall, Virginia Beach, Virginia were collected in order to evaluate the effect of the outfall on benthic foraminifera. The top centimeter of sediment was analyzed for living and total (live plus dead) foraminiferal densities, chlorophyll a, sediment grain size, ATP, percentage of silt and clay, and total organic carbon. Bottom water salinity, temperature, and ammonia were also analyzed.

Living foraminiferal density increased from March to July and with distance from the outfall. Total foraminiferal density also increased from March to July. The set of environmental variables was …

The Food Habits Of Ictalurus Natalis, Centrarchus Macropterus And Perca Flavescens In Lake Drummond In The Dismal Swamp Of Virginia, Steven C. Russell

Biological Sciences Theses & Dissertations

The food habits of yellow bullhead catfish (Ictalurus natalis), yellow perch (Perea flavescens), and fliers (Centrarchus macropterus) in Lake Drummond in the Dismal Swamp were examined. Seventy yellow bullheads (42 mm to 255 mm), 136 yellow perch (41 mm to 280 mm), and 110 fliers (81 mm to 140 mm), were collected with gill nets, traps, seins, and electro-fishing gear from June through November of 1975. Frequency of occurrence and volumentric data were used to evaluate the relative importance of food items. Spearman's rank correlation coef­ficient was determined from the data to test differences …

A Comparative Study Of A Salt Water Impoundment With Its Adjacent Tidal Creek Pertinent To Culture Of Crassostrea Virginica (Gmelin), William D. Anderson Iii

OES Theses and Dissertations

Certain physical, chemical and biological characteristics associated with the culture of subtidal Crassostrea virginica were assessed in a salt water impoundment and its adjacent tidal feeder creek. Large seed oysters (initial y = 57.3 mm) were transferred from a somewhat polluted estuary of Charleston Harbor to floating, mid-water, and bottom hardware cloth trays (200/tray) in a four hectare pond. Identical trays at comparable depths were placed in the tidal creek and each location was sampled monthly for growth and survival. Surface and bottom water samples were collected weekly throughout the study and hourly during four seasonal 25 hour stations. Standard …

An Intensive Study Of The Spring Phytoplankton Bloom In Lynnhaven Bay, Virginia, Donald Lynn Webster

Biological Sciences Theses & Dissertations

Phytoplankton samples were collected biweekly from two stations, one in each branch of Lynnhaven Bay, Virginia over the period January 24 to June 9, 1975. Water temperature, salinity, pH, oxygen, ammonia, urea, reactive nitrite, reactive nitrate, reactive phosphorus, productivity, transparency, and weather conditions were recorded concurrently. Data were manipulated employing analysis of variance, simple correlation, correlation matrices, and forward stepwise multiple regression. Total phytoplankton numbers exhibited a unimodal pattern. A pre-bloom pulse was recorded at both stations in late February. The spring bloom lasted from April through May. Phytoplankton growth was primarily a function of nutrient supply and temperature. Nutrient …

Sex Pheromone Activity In The Foveal Glands In The Two Species Of Ixodid Ticks: Dermacentor Andersoni (Stiles) And Dermacentor Variabilis (Say), Lynn Anne Collins

Biological Sciences Theses & Dissertations

Sex pheromone activity in the foveal glands of female Dermacentor andersoni (Stiles) and Dermacentor variabilis (Say) is described. Autoradiography revealed the accumulation of ³⁶Cl in the foveal glands of females during their first 120 hours of feeding on a ³⁶Cl-labeled host. Emissions collected from these females contained a ³⁶Cl-labeled product. This product, however, was absent in emissions collected from the same females with their foveae dorsales obstructed.

Accumulation of ³⁶Cl in the foveal gland, in conjunction with the release of a ³⁶Cl-labeled product through the foveae dorsales, suggest localization of sex pheromone activity within these …

Kinetic And Binding Studies On L-∝-Glycerophosphate Dehydrogenase, Paul Richard Rosevear

Chemistry & Biochemistry Theses & Dissertations

The properties of the coenzyme, NAD, binding site of chicken muscle L-∝-glycerophosphate dehydrogenase were studied. Adenosine monophosphate, adenosine diphosphate and adenosine diphosphoribose were sha.vn to inhibit the enzyme competitively with respect to NAD. The presence of adenosine, pyrophosphate and ribose regions in the coenzyme binding site of the enzyme was suggested by the inhibitor constants obtained for these compounds.

Disodium monoalkyl phosphates, n-butyl to n-dodecyl phosphate, inclusive, were also shown to inhibit the L-∝-glycerophosphate dehydrogenase catalized reaction competitively with respect to NAD. A positive chain length effect was observed in the binding of these compounds to the enzyme, suggesting the …

Studies Of The Purification Of Nad-Dependent And Nadp-Dependent Isocitrate Dehydrogenase By General Ligand Affinity Chromatography, Geoffrey H. Bertkau

Chemistry & Biochemistry Theses & Dissertations

Purification of Yeast NAD⁺-dependent isocitrate dehydrogenase was partially successful when utilizing an NAD⁺-ribosyl-hydrazide Sepharose affinity chromatography column. Although no specific elution system was found, a maximum purification of sixty-five fold was achieved with 0.5M KCl elution. A 38-fold purification was obtained when 20mM NAD⁺ was employed for elution.

Yeast and calf liver NADP⁺-dependent isocitrate dehydrogenase were also found to bind to NAD⁺-ribosyl-hydrazide Sepharose as well as the NAD⁺-dependent enzyme. Again, elution was achieved with either o,5M KCl or 20 mM NADP⁺.

Ammonium sulfate fractionation decreased the yield …

Structural Comparisons Of Four Plant Communities In The Great Dismal Swamp, Claire Virginia Dabel

Biological Sciences Theses & Dissertations

Four plant communities in the Great Dismal Swamp, located on the coastal plain in southeastern Virginia, were sampled and estimates of density, basal area and aboveground standing crop biomass were determined, Biomass was estimated as 229,800 kg/ha in the Chamaecyparis thyoides community and as 344,500 kg/ha in the Taxodium distichum community. Biomass for the two hardwood communities, the

Acer-Nyssa community and mixed hardwood (Quercus-Nyssa-Acer-Liriodendron-Liquidamber) community was estimated as 195,700 kg/ha and 194,600 kg/ha respectively and did not differ significantly (P

An Investigation Of The Nucleotide Cyclic 3'5' Adenosine Monophosphate In Young Hypocotyls Of Pisum Sativum L., Gary Marsh Hilton

Biological Sciences Theses & Dissertations

Concentrations of cAMP increased with time in pea hypocotyls assayed at 24 hour intervals over a period of four days. The highest concentration was 20.1 pmoles/mg protein which occurred in 96 hour hypocotyl tips. Ninety-six hour root bodies (dissected region between tip and embryo) achieved a concentration of 8.6 pmoles/mg. Concentrations of cAMP in embryos remained constant around one pmole/mg for three days and values doubled on the fourth day.

Hypocotyls, after germination for 72 hours, were dissected and incubated for various lengths of time in water and 3xl0^-8M IAA. Tips incubated for two hours showed a tenfold …

Improved Techniques For Marking, Night-Viewing, And Aging White-Tailed Deer, Odocoileus Virginianus, Barnard Hodge Powell

Biological Sciences Theses & Dissertations

These studies were concerned with the development of methods to assist investigators of wildlife population research. Specifically they involve the description of (1) A device which enables an investigator to mark deer at medium range using specially modified darts fired by conventional tranquilizer rifles, (2) Construction of infrared viewers and light sources using surplus equipment and (3) Studies to develop a method of making permanent deer dental impressions under field conditions.

Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoretic Studies Demonstrating Cytoplasmic Protein Components Unique To The Mycelial Phase And To The Yeast Phase Of Candida Albicans, Theresa Catherine Burgess

Biological Sciences Theses & Dissertations

Blastospore and mycelial cytoplasmic extracts from four strains of Candida albicans, varying in virulence, were compared by sodium dodecyl sulfate (SDS) polyacrylamide disc gel electrophoresis Cytoplasmic extracts were made by disrupting the cultures in a Braun homogenizer followed by ultracentrifugation. The supernatant fractions were then resolved on polyacrylamide gels containing SDS. Each of the preparations yielded a distinct pattern. There were mycelial specific and blastospore specific protein bands. There were more components resolved from the blastospore phase cytoplasmic extracts than from the mycelial phase cytoplasmic extracts. There were bands common to both phases. These results demonstrate protein components unique …

Variation In The Ecology Morphology And Behavior Of The Troglobitic Amphipod Crustacean Crangonyx Antennatus Packard (Crangonchidae) From Different Habitats, Gary Wayne Dickson

Biological Sciences Theses & Dissertations

Populations of the troglobitic amphipod Crangonyx antennatus living in two distinct aquatic habitats were examined for possible variations in their ecology, morphology and behavior. Collections were made seasonally for one year in six Lee Co., Virginia caves, three containing mud-bottom, drip pools and three with small gravel stream habitats. Environmental parameters thought to influence population variation were quantitatively and qualitatively recorded in each of the six caves.

Amphipod densities, body length, female maturity, clutch number and stream washout rates were found to be greater in drip pool habitat populations. Variation was also observed in body coloration and antennal segment-body length …

The Natural History Of The Naked Goby Gobiosoma Bosci (Perciformes: Gobiidae), Leonard L. Nero

OES Theses and Dissertations

The purpose of this study was to contribute to the knowledge of re ­ production, growth, age, abundance, habitat preference, feeding habit and behavior of the naked goby, Gobiosoma Bosci.

Monthly collections were made from January through December. 1974 in Deary Cove a apart of the Lynnhaven estuary, Virginia Beach, Virginia. All fish were measured and sexed in the field.

Most were returned to the population, the remainder. were removed for food habit analyses, determination of fecundity, degree of gonadal maturity and aging. Habitat­ preference and abundance were determined in the field.

Gobiosoma Bosci appears to be an annual …

A Batch Method For The Isolation Of Immunogenically Pure Igg, Iga, And Igm From Human Sera, Samuel Christopher Powell

Chemistry & Biochemistry Theses & Dissertations

A method is described for preparing an enriched fraction of the three major antibodies, immunoglobulins IgG, IgA and IgM, from normal human serum by a batch method. Selective precipitation of these proteins was carried out in successive steps using polyethylene glycol (PEG) of average molecular weight 6,000 at concentrations of 7%, 12% and 14% (16) . Zinc sulfate (10 mM) was used to remove highly immunogenic alpha₂ macroglobulin from the IgM fraction. IgA, IgM and IgG were further purified by adsorption methods using DEAE-cellulose. Quantitation and qualitation of the enriched fractions showed nearly complete separation of each of the …

Test Variation In The Sand Dollar, Mellita Quinquiesperforata (Leske), George Glynn Rountree

OES Theses and Dissertations

Mellita quinguiesperforata is a common member of the benthos along the eastern coast of North America. This study considers variation of the test within the species, specifically: 1) variation in test weight, 2) variation in the length-width relationship, and 3) the average number of pore pairs in the ambulacral petals.

A total of 844 sand dollars from three localities was utilized: 1) Pass-a-Grill Beach, St. Petersburg, Florida,2) Bird Shoal, Beaufort, North Carolina, and 3) Fisherman Island, Virginia.

Animals were measured for length and width and the dry weight determined. Prediction equations for the size-weight relat1onships were computed for the three …

The Distribution Of Submersed Hydrophytes In Currituck Sound, Steven Harry Davis

Biological Sciences Theses & Dissertations

The rooted submergent flora of Currituck Sound, North Carolina was determined, and the distributions of its species investigated with respect to salinity, substrate texture, depth, pH, turbidity and species co-occurrence. Eight species of hydrophytes were found: Potamogeton pectinatus, Fotamogeton perfoliatus, Ruppia maritima, Zannichellia palustris, Najas guadalupensis, Elodea nuttallii, Vallisneria americana, and Myriophyllum spicatum. The mean salinities at which each species occurred ranged from 1.0 ppt for E. nuttallii to 3.1 ppt for Z. palustris, Ruppia maritima, Z palustris and V. americana were the only species that commonly occurred on coarse-grained substrates. Elodea nuttallii and N. guadalupensis …