Digital Commons Network^™

Model-Based Network Reconstruction From Cascade Dynamics, Katherine Irena Chwistek

Graduate Research Theses & Dissertations

Network representation provides a natural framework for the study of real world complex systems. From social networks and animal groups to interneuronal communications and power grid systems, complex patterns of interaction can be captured and modeled using networks in a simple mathematical form. In many cases, however, a faithful network representation of the system is not readily available. For this reason, network reconstruction has become a growing topic of interest in recent years, the goal of which is to discover the hidden interaction patterns among individuals by fitting input-output data from multiple experiments to candidate network topologies.

During a cascade, …

Assessing The Impact Of Mother’S Perception Of Support On Breastfeeding Intensity Through Six Months, Kathryn Doyle Palomares

Graduate Research Theses & Dissertations

INTRODUCTION: Expert recommendations instruct mothers to exclusively breastfeed for infant’s first 6 months of life. While many mothers intend to breastfeed, mothers frequently cite early breastfeeding difficulties as a leading cause of breastfeeding cessation. The large discrepancy between a mother’s intentions for exclusive breastfeeding and the actual rates warrants further investigation. OBJECTIVES: The current research study utilized data from the Infant Feeding Practices Study II to (1) to understand how the mother’s perception of breastfeeding support in the presence of breastfeeding difficulties affects breastfeeding intensity. METHODS: Multinomial logistic regression utilizing data from the Infant Feeding Practices Study II. At each …

Influence Of Maternal Eating Attitudes, Behaviors, And Fat Talk On Adolescent, Non-Elite, Competitive-Level Gymnasts, Madisyn Rozner

Graduate Research Theses & Dissertations

Adverse eating attitudes and eating behaviors describe thoughts and actions related to food and body image that increase risk for development for an eating disorder. The term, “fat talk” was defined as, “cultural meanings of talk about weight” and has been used to assess preoccupation with the cultural stigma of weight. Understanding risk factors for the development of adverse thoughts and actions in adolescence is especially important, as disordered cognitions and behaviors often appear in early adolescence. Maternal modeling, children modeling their actions or attitudes based on their mothers, plays a role in the level of risk. Similarly, gymnastics, an …

Understanding The Effects Of Food Security And Snap Participation On The Fruit And Vegetable Intake Of Older Adults, Alexandra Sarkisian

Graduate Research Theses & Dissertations

This thesis focuses on understanding what impact the barrier of food security has on the fruit and vegetable intake of older adults. Food insecurity - lacking consistent access to enough food for an active, healthy lifestyle - is an issue that affects 1 in 9 Americans, and can have an impact on one’s diet and potentially overall health. Using secondary data collected through the 2013-2014 National Health and Nutrition Examination Survey, this study assessed the intake of fruits, vegetables, as well as the variety of produce consumed. This was then analyzed in order determine how the intake of these foods …

Mobile Apps For Type 2 Diabetes In A Low Socioeconomic Status Population, Kaitland Rae Woelky

Graduate Research Theses & Dissertations

Background: Individuals of low socioeconomic status (SES) are disproportionately at risk for developing type 2 diabetes (T2DM) compared to their higher SES counterparts. Smartphone apps are increasingly used as a tool to compliment current diabetes education and have the ability to increase prevention and diabetes education in health disparate communities. The objective of this study was to identify the quality of nutrition and health related apps frequently recommended by diabetes educators to compliment current T2DM education programs for patient of low SES.

Methods: Clinicians (n=120) were recruited through two diabetes and dietetic professional groups to complete an online App Identification …

Single-Domain Vhh Antibodies As Protein Stabilization Modules, Nourhan Mohamed Disouky Mostafa

Graduate Research Theses & Dissertations

VHH domains are the antigen-binding units found in heavy-chain-only antibodies from the Camelidae family (e.g., camels and llamas). They are considered a unique minimalist antibody scaffold due to the lack of a variable light chain domain. Despite having half the antigen-binding interface of conventional antibodies, these VHH antibodies bind their target antigens with affinities comparable to conventional antibodies. This project explored how flexible linkers connecting an anti-GFP VHH (αGFP VHH) domain to its antigen (EGFP) enhanced antigen stability and influenced oligomeric assembly. It was hypothesized that linking the anti-GFP VHH antibody to its target antigen (EGFP) will increase the local …

Dispelling The Mirage Of C4-Driven Drought Tolerance In Poaceae: A Phylogenomic Study, Matthew P. Nissenbaum

Graduate Research Theses & Dissertations

Climate change has been considered a key driver for adaptations in water usage efficiency (WUE) in plants. Perceptions persist that C4 photosynthesis is indicative of some level of WUE. Here, we examined the interaction and evolution of WUE within the grass family, Poaceae, as it pertains to photosynthetic pathway. Unique to this study is the magnitude of taxa sampled, presence of intrageneric sampling, and use of complete plastid genomes (plastomes) for phylogenomic analyses.

310 species were sampled, including 26 with newly sequenced plastomes, which were assembled de novo from next-generation sequencing data. Additional plastomes were downloaded from the National Center …

Bioinformatic Anlayses Of Identified But Uncultivated Microbial Dark Matter Organisms Using Archival Single Amplified Genomes (Sags), Daniel Adam Nuccio

Graduate Research Theses & Dissertations

This project characterizes two independent, previously undescribed microbial dark matter organisms from the candidate phyla JL-ETNP-Z39 and NBK19. These characterizations are based on seven single amplified genomes (SAGs) for JL-ETNP-Z39 and six SAGs for NBK19. All SAGs used were provided by the Bigelow Laboratory for Ocean Sciences Single-Cell Genomics Center (scgc.bigelow.org). Bioinformatic analyses using a combination of publicly available software and custom scripting written in the computer programming languages Python and R were carried out to determine the basic genomic properties, evolutionary relatedness, representative environmental pangenomes, and metabolic properties of these organisms. Analyses indicated that the examined JL-ETNP-Z39 SAGs are …

A Bioinformatic Approach To Exploring Land Plant Evolution, Lauren Margaret Orton

Graduate Research Theses & Dissertations

Today, researchers are inundated with an overwhelming amount of data due to advances in DNA sequencing technologies. This has benefited the fields of both bioinformatics and phylogenomics substantially. With the ability to increase sampling at both the taxonomic and molecular levels, we are now able to produce robust phylogenomic reconstructions and better discern relationships between taxa.

The goals of this dissertation are in two distinct parts: 1) to advance the understanding of land plant terrestrialization and evolution through examination of the closest relative to the land plant lineage, a species of green algae; and 2) to explore evolution among a …

Evaluation Of The Coordinated Approach To Child Health (Catch) Program In Third Through Fifth Graders In Northern Illinois, Paulina Karecka

Graduate Research Theses & Dissertations

Background: The Coordinated Approach to Child Health (CATCH) is a school-based education program designed to improve dietary habits and increase physical activity among children and adolescents based on the Social Cognitive Theory (SCT). The objective of this study was to evaluate the effectiveness of CATCH program, delivered by dietetic interns and Northern Illinois University (NIU) students, to 3rd-5th graders in Northern Illinois, in increasing their nutrition knowledge and healthy choices behavior. Methods: In total, 167 elementary school children in grades 3-5 in Northern Illinois participated in a non-experimental program evaluation study. We delivered 6 CATCH lessons throughout the academic year …

The Effects Of In-Season Training Changes On Body Composition In Division I Collegiate Football Players, Kayla Emory

Graduate Research Theses & Dissertations

Body composition quantification in athletics can be used as a tool for developing a training program and monitoring progress in developing ideal body composition for optimal performance. Commonly, athletes are concerned with the amount of fat free mass (FFM) and fat mass (FM) they have, since those variables have been commonly linked to reduced performance and increased injury risks. The game of football is constantly evolving and so is the need for players to be bigger, stronger, and faster. Most research has investigated the body composition changes in football players as a whole team during an off-season, however few have …

Canine And Criminal Bias: The Relationship Between Stereotypes And Perceptions Of Punishment, Jamie I. Thomas

Graduate Research Theses & Dissertations

In this thesis I provide an analysis of punitive perceptions of animal crimes and argue that the lack of value seen in animal’s lives affects perceptions of punishment for animal crimes. Specifically, I examine the role of animal attitudes and race on perceptions of crimes against dogs and will address the following question with a vignette survey design: How do racial stereotypes interact with dog breed stereotypes when considering punishment for animal crime? This research expands on factors that influence perceptions of punishment of animal crime, including racial differences that may affect attitudes about animals. This study contributes to the …

Categorization Of Orthologous Gene Clusters In 92 Ascomycota Genomes Reveals Functions Important For Fungal Phytopathogenicity, Daniel Curtis Peterson

Graduate Research Theses & Dissertations

Ascomycota fungi are mostly saprophytic in nature, living off dead or decaying matter. However, a small subset of these fungi possess the ability to infect live hosts and cause disease while obtaining nutrients. Phytopathogenic Ascomycota are responsible for substantial economic losses each year, destroying valuable crops. Previous genomic studies have been conducted to annotate sequenced genes and proteins of these fungi with predicted functions, and those annotations are publicly accessible through online databases. Through these means, several genes have been identified to play a role in pathogenicity in some agriculturally relevant fungi. Through a bioinformatic analysis approach the present study …

Diet Of Spalangia Cameroni (Hymenoptera: Pteromalidae), A Parasitoid Of Filth Flies, Elizabeth Taylor

Graduate Research Theses & Dissertations

Parasitoid wasps are often part of the rich invertebrate communities in decaying organic matter where their hosts are found. However, little is known about what adults of these parasitoids eat. Most parasitoid wasps parasitize herbaceous insects, so nectar from flowers often is readily available. The present study examined feeding by adult Spalangia cameroni Perkins (Hymenoptera: Pteromalidae), a species that burrows through manure, where it finds and parasitizes pupae of filth flies. Feeding on host fluids occurs in many parasitoid wasps, including S. cameroni. In the laboratory, these wasps also readily feed on honey or a sucrose solution, which increases their …

Wildlife Ecology And Conservation In A Suburban Preserve Matrix: Applications Of Long-Term Monitoring Data, John Peter Vanek

Graduate Research Theses & Dissertations

Wildlife is important to humans, offering economic, nutritional, ecological, and socio-cultural value. However, vertebrate biodiversity is being lost at rates 100 times greater than the background extinction rate, threatening human livelihood via the loss of ecosystem services. These human-induced species losses are primarily due to habitat destruction, which is increasingly tied to urban development. Conservation of wildlife resources is therefore imperative in a world where more than 50% of the human population, and 80% of people living in the United States, now live in urban areas. In this dissertation, I demonstrate how monitoring data can be applied to answer questions …

Association Of Various Physiological And Fitness Markers To Body Fat Percentage In Physically Inactive Adults, Anthony Mckee

Graduate Research Theses & Dissertations

Obesity can dramatically and negatively affect many aspects of normal physiological functioning. Obesity is defined as abnormal or excessive body fat accumulation that presents a risk to health. Body mass index (BMI) is a widely used approach to quantify body fat in large group settings; however, this method has significant shortcomings with accuracy and estimation. More direct measures such as bioelectrical impedance analysis (BIA) can provide a more reliable and accurate assessment of body fat mass and, therefore reduce the shortcomings of BMI when assessing possible associations with health- and performance-related variables. A plethora of research studies has been conducted …

Characterization Of The Putative Polysaccharide Synthase Cpsa And Its Effects On The Virulence Of The Human Pathogen Aspergillus Fumigatus, Binita Nepal

Graduate Research Theses & Dissertations

The fungus Aspergillus fumigatus is a ubiquitous opportunistic human pathogen capable of causing a life-threatening disease called invasive aspergillosis, or IA, with an associated 40–90% mortality rate in immunocompromised patients. Of the approximately 250 species known in the genus Aspergillus, A. fumigatus is responsible for up to 90% of IA infections. This study focuses on examining the role of the putative polysaccharide synthase cpsA gene in A. fumigatus virulence. Additionally, we evaluated its role in cellular processes that influence invasion and colonization of host tissue. Importantly, our results support that cpsA is indispensable for virulence in A. fumigatus infection of …

Depression And Anxiety In A Female Adolescent Population With Sources Of Social Support Latent Profiles, Kathleen Kelly

Graduate Research Theses & Dissertations

Social support is associated with various outcomes such as depression and anxiety. Less work has investigated how support received from various sources cumulatively is associated with internalizing symptoms. Research suggests that above other sources, parents and peers are the most strongly associated with internalizing outcomes. Looking at female adolescents specifically, the current study explored 1) what patterns of social support across sources emerge in a female adolescent sample, and 2) how do depressive and anxiety symptoms vary based on those patterns of social support? The study utilized Latent Profile Analysis (LPA) on data collected from a female high school sample …

Engineering Ph-Dependent Antibody Interactions Through Linked Protonation Events In Interdomain Interfaces, Hyeyoung Eom

Graduate Research Theses & Dissertations

Engineered antibodies are frequently used in life science applications, such as therapeutics and clinical diagnostics. These customized antibodies typically possess enhanced binding affinity and/or specificity, yet these interactions are only one-way binding events that cannot be easily controlled without extreme changes in conditions, such as pH, denaturant, and/or temperature that likely irreversible damage the antibody and antigen. Here, we explore the design of proton-linked binding events in the context of both antibody/hapten (i.e., a low molecular weight ligand target) and antibody/antigen complexes. Specifically, this work looks to overcome the challenges of engineering pH-dependent small ligand/protein interactions, as well as develop …

Wading In A Glycan Sea: Investigations Of, And Developing Bioinformatic Tools For, Carbohydrate Active Enzymes And Gene Clusters In Prokaryotes, Catherine Ann Ausland

Graduate Research Theses & Dissertations

Carbohydrates are immensely important biomolecule found in all organisms on earth, serving roles in structure, energy storage, protection and/or cell signaling. Carbohydrates are composed of an immense diversity of linkages, monosaccharide moieties and substitutions making them likely the most diverse biomolecule on earth, as well as the most abundant, being synthesized by plants and algae. Carbohydrates are synthesized and degraded by all organisms on earth through the expression of carbohydrate active enzymes, or ‘CAZymes’. In bacteria, CAZymes have been characterized to cluster in with transporters, regulators and/or other genes in so-called ‘polysaccharide utilization loci’, or PULs, to degrade carbohydrate substrates …