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Macroecological Patterns Of Frugivorous Fishes’ Diversity (Serrasalmidae) In The Amazon Drainage Basin, Karold Vivianna Coronado Franco
Macroecological Patterns Of Frugivorous Fishes’ Diversity (Serrasalmidae) In The Amazon Drainage Basin, Karold Vivianna Coronado Franco
Theses and Dissertations
The Amazon River drainage basin is known as the most biodiverse region in the world. Regarding freshwater fish, this region contains around 15% of the world's fish richness. The fish family Serrasalmidae have a variety of feeding preferences including Pacus (herbivores) and Piranhas (carnivores). Fruit eating fish species are evolutionarily the oldest species in the family and have a mutualistic interaction with flooded forests. Considering the diversity of feeding habits of the Serrasalmidae family, it represents an excellent model to study ecological questions related to factors that influence the spatial distribution of species and factors that contribute to the maintenance …
The Response Of An Avian Community To Intercropping And Forest Management Practices In A Private Working Pine Forest, Rebecca Doyne Bracken
The Response Of An Avian Community To Intercropping And Forest Management Practices In A Private Working Pine Forest, Rebecca Doyne Bracken
Theses and Dissertations
Within managed pine forest systems, a plethora of bird species exist throughout the lifecycle of a stand akin to what may be experienced through post-disturbance regeneration in a natural forest system. I sought to address how breeding avian communities shift across time in response to stand aging and forest management, evaluate species-specific responses to stand conditions, investigate the responses of at-risk avian species to forest management, and determine avian non-breeding, over-wintering presence in a managed loblolly pine (Pinus taeda) forest. I conducted breeding bird point count and vegetation surveys within five stands of privately owned and managed pine forest in …
Surface Modification Of Wood Using Nano-Sized Titania Particles Coated By Liquid-Precursor Flame Spray Pyrolysis, Ganesh Sedhain
Surface Modification Of Wood Using Nano-Sized Titania Particles Coated By Liquid-Precursor Flame Spray Pyrolysis, Ganesh Sedhain
Theses and Dissertations
Wood is a renewable resource and versatile material used in tasks ranging from tools and furniture to advanced engineering structures. Although wood is light, mechanically robust, environmentally friendly, and abundant, some inherent properties of wood, such as degradation due to moisture and UV radiation from sunlight, are less desirable for extended service life and dimensional stability. In this dissertation, a novel surface modification of wood is explored by depositing nano-sized titania particles on wood veneers and cross-laminated timber (CLT) blocks by liquid-precursor flame spray pyrolysis to confer reversible wettability switching and enhanced durability to UV irradiation. The reaction between a …
Activity Level Of Helicoverpa Armigera Nucleopolyhedrovirus (Hearnpv) And Feeding Habits Of Helicoverpa Zea Larvae After Ingestion Of Hearnpv In Mississippi Soybean, Judge Tyler Fortenberry
Activity Level Of Helicoverpa Armigera Nucleopolyhedrovirus (Hearnpv) And Feeding Habits Of Helicoverpa Zea Larvae After Ingestion Of Hearnpv In Mississippi Soybean, Judge Tyler Fortenberry
Theses and Dissertations
HearNPV was applied to a soybean field and evaluated for residual efficacy at different time intervals (0, 12, 24, 36, 48, and 72 hours) after application in laboratory bioassays with treated leaves. Larval mortality was rated at five, seven and twelve days after larval infestation. Larval mortality increased as the number of days after infestation increased. To evaluate the feeding habits of Helicoverpa zea at the 2nd and 3rd instar, HearNPV exposed larvae and untreated larvae were weighed at zero (before diet exposure), four, and seven days. At four days after exposure, larval weight gain of …
Evaluation Of Italian Ryegrass And Palmer Amaranth Control In Mississippi, Johnson Harris Hughes
Evaluation Of Italian Ryegrass And Palmer Amaranth Control In Mississippi, Johnson Harris Hughes
Theses and Dissertations
Italian ryegrass is a problematic weed in Mississippi corn production due to the development and proliferation of glyphosate resistance. Studies were conducted to assess Italian ryegrass control prior to planting using herbicides. Effects of fall and spring applied burndown herbicide applications for Italian ryegrass control and subsequent corn grain yield were investigated at the R.R. Foil PSRC in Starkville, MS, at the Coastal Plain Experiment Station in Newton, MS, and the Black Belt Experiment Station in Brooksville, MS on soil textures ranging from sandy loam to silt clay loam. A fall preemergence (PRE) application of S-metolachlor + metribuzin followed …
The Influence Of Overwatering, Underwatering, And Waterlogging On The Growth Of Kale (Brassica Oleracea Var. Acephala), Skyler R. Brazel
The Influence Of Overwatering, Underwatering, And Waterlogging On The Growth Of Kale (Brassica Oleracea Var. Acephala), Skyler R. Brazel
Theses and Dissertations
The Intergovernmental Panel on Climate Change has predicted that there will be increases in precipitation and heat-induced drought events globally. Information on kale response to waterlogging is minimal. The purpose of this project was to identify the response of kale to three treatments of water stress: underwatering, overwatering, and waterlogging. Plant pigments analyzed displayed a varied response to underwatering and overwatering, with concentrations changing with maturity but with reductions and no changes, respectively, at full harvest maturity. Glucosinolate concentrations were also influenced by maturity and increased under waterlogging and underwatering but no differences with overwatering. Overall, water stress to any …
Marginal Agricultural Land Identification In The Lower Mississippi Alluvial Valley, Prakash Tiwari
Marginal Agricultural Land Identification In The Lower Mississippi Alluvial Valley, Prakash Tiwari
Theses and Dissertations
This study identified marginal agricultural lands in the Lower Mississippi Alluvial Valley using crop yield predicting models. The Random Forest Regression (RFR) and Multiple Linear Regression (MLR) models were trained and validated using county-level crop yield data, climate data, soil properties, and Normalized Difference Vegetation Index (NDVI). The RFR model outperformed MLR model in estimating soybean and corn yields, with an index of agreement (d) of 0.98 and 0.96, Nash-Sutcliffe model efficiency (NSE) of 0.88 and 0.93, and root mean square error (RMSE) of 9.34% and 5.84%, respectively. Marginal agricultural lands were estimated to 26,366 hectares using cost and sales …
Determining The Impact Of Post-Harvest Water Management On Chironomid Abundance, Agrochemical Biomass And Potential Trophic Biomagnification, Mason Thomas
Theses and Dissertations
Agriculture has diminished shorebirds’ natural habitat in the Mississippi Alluvial Valley. Remaining natural stopover sites are supplemented with agricultural fields during the fall and winter. This study evaluates the impact of 4 different post-harvest water management strategies on shorebird food abundance and potential agrochemical biomagnification. Chironomid samples estimated abundance, biomass, and chironomid agrochemical concentration in each field. A risk assessment of agrochemical biomagnification to shorebirds was made across all treatments. Of treatments represented on all study sites, winter treatment had greatest chironomid abundance and biomass. Models indicated that days since flood initiation, start date, and temperature are significant predictors of …
Interspecies Differences In Organophosphate Anticholinesterase Inhibition Potency And Reactivation Using Novel Oximes, Katie Elizabeth Strickland
Interspecies Differences In Organophosphate Anticholinesterase Inhibition Potency And Reactivation Using Novel Oximes, Katie Elizabeth Strickland
Theses and Dissertations
Organophosphates are insecticides which result in acute adverse signs when exposed at toxic doses by animals and lead to death if left untreated. The current treatment for organophosphate toxicity includes atropine and the federally approved oxime 2-PAM. However, 2-PAM is not very effective at crossing the blood brain barrier which results in prolonged inactivation of acetylcholinesterase, which is the primary target of organophosphates, in the brain even after administration. The novel oximes, Oxime 15 and Oxime 20, are able to cross the blood brain barrier and reactivate the inhibited acetylcholinesterase. In this experiment with six animal species frequently used in …
Physiology Of Human-Equine Interaction During Substance Withdrawal, Molly Marie Friend
Physiology Of Human-Equine Interaction During Substance Withdrawal, Molly Marie Friend
Theses and Dissertations
Psychotherapy incorporating equine interaction (PIE) is emerging as an effective treatment for substance use disorder (SUD), but research is limited concerning physiological impacts during substance withdrawal. This study investigated impacts of PIE on salivary cortisol concentrations, heart rate, anxiety, and depression during SUD withdrawal. Heart rate and cortisol concentrations were measured in horses to determine potential human-horse coupling. Saliva samples and heart rates were collected pre and post PIE from residential SUD patients (n=18) and their therapy horses (n=4) during and following the withdrawal period. Participants (n=10) also completed a survey measuring anxiety and depression (P = 0.05). A …
Determining The Importance Of Propagule Pressure And Dispersal Mechanisms For The Establishment And Spread Of Crapemyrtle Bark Scale, Acanthococcus Lagerstroemiae Kuwana (Hemiptera: Eriococcidae), Erika Renee Wright
Theses and Dissertations
Non-native scale insects can be economically and ecologically important pests of urban forests. Crapemyrtle bark scale (CMBS), Acanthococcus lagerstroemiae Kuwana (Hemiptera: Eriococcidae), is a scale that causes declines in plant vigor for a popular ornamental tree in the southeastern United States, crapemyrtle (Lagerstroemia spp.). CMBS has spread rapidly throughout the Southeast and, more recently, into the Middle Atlantic. Despite problems associated with CMBS, important aspects of its ecology, such as the role of propagule pressure in establishment and the relative importance of mechanisms for between-tree dispersal, are not well-understood. We used field and laboratory studies to investigate these aspects of …
Interactive Effects Of Bark Beetles, Ophiostomatoid Fungi, And Subterranean Termites On Wood Decomposition And The Biogeochemical Cycling Of Pine Forests, Kimberlyn Pace
Theses and Dissertations
A frequent source of pine tree mortality in recent years can be attributed to pine bark beetles and their vectored Ophiostomatoid fungi, an organism that has been observed to attract subterranean termites that preferentially recruit to this downed woody debris. This interaction may significantly modify biogeochemical fluxes in bark beetle mass mortality events, but studies are often limited to singular regions or single pine-dominated ecosystems. Two studies were designed to test the interactive effects of these associations on terrestrial biogeochemical cycles and decomposition processes, the first using replicated field trials in Mississippi, Arizona, and Honduras across two years and the …
Impact Of Daily Sweet Potato Ingestion On Alterations To The Gut Microbiome, Cholesterol, And Exercise Performance, Johneric William Smith
Impact Of Daily Sweet Potato Ingestion On Alterations To The Gut Microbiome, Cholesterol, And Exercise Performance, Johneric William Smith
Theses and Dissertations
The importance of the gut microbiome is being explored in relation to multiple facets of health and performance. The purpose of this study was to explore the impact of frequent sweet potato ingestion on alterations of the gut microbiome which might in turn alter cholesterol levels and exercise performance. Thirteen recreationally trained males had fecal and blood samples collected and exercised to fatigue with and without exogenous carbohydrate ingestion prior to and following 42 days of daily sweet potato ingestion. Bacterial communities were extracted from fecal samples and bacterial DNA were sequence. Blood samples were analyzed for total cholesterol, low-density …
Evaluation Of Nutrient Digestibility Of Weaned Calves From Early And Late Shedding Dams, Jennifer Keele
Evaluation Of Nutrient Digestibility Of Weaned Calves From Early And Late Shedding Dams, Jennifer Keele
Theses and Dissertations
Researchers have investigated several factors that could alter fetal growth, including nutrient restriction (Valiente et al., 2021), hair shedding (Gray et al., 2011), and extreme hot and cold temperatures (Davidson et al., 2022). Hot temperatures and increased humidity percentages in the southeast United States caused researchers to investigate the hair coats of Angus cattle in the commercial production setting. An improvement in fiber digestibility and calf birth and weaning weights has been observed in Angus dams that shed 50% of the winter hair coat by May (Gray et al., 2011; Burnett et al., 2021). Our objective of this experiment was …
Expanding The Market Of Biomaterials, Franklin Quin Jr.
Expanding The Market Of Biomaterials, Franklin Quin Jr.
Theses and Dissertations
Biomaterials such as wood and bamboo are in high demand as a building material with the push for building with green technology. The wood product industry accounts for approximately 4% of the total U.S. manufacturing GDP (Gross Domestic Product), which is more than $100 billion. The industry supports over 752,000 full-time equivalent jobs, most of which are in rural areas where employment opportunities are limited. The estimated global market value of bamboo is estimated to be $60 billion annually. This research will explore the use of wood and bamboo in different end use products. The objectives of this research will …
Examining Physiological And Photosynthetic Effect Of Led Lighting On Lettuce And Kale Microgreens, James Travis Blanche
Examining Physiological And Photosynthetic Effect Of Led Lighting On Lettuce And Kale Microgreens, James Travis Blanche
Theses and Dissertations
The objective of these studies was to observe the effects of LED lighting treatments on physiological parameters of lettuce and microgreens. Four different lighting treatments; 1) Physiospec (white), 2) Red, 3) DR9 (25:75 Blue:Red), and 4) Anthospec (at 20/80 Blue:Red); were used in this experiment. Two experiments were conducted: 1) Examining the effects of LED lights on lettuce yield and photosynthetic activity and 2) The effects of switching lighting regiments halfway through the growth cycle on Kale microgreen yield and color. Physiospec lighting was found to promote the highest yield and photosynthetic activity among the four treatments for lettuce. It …
Spatial Conservation Planning In The Southeastern United States: Alignments And Opportunities, Bradly Stewart Thornton
Spatial Conservation Planning In The Southeastern United States: Alignments And Opportunities, Bradly Stewart Thornton
Theses and Dissertations
Conservation managers and planners need the ‘best available science’ to support robust and defensible decisions, ensuring that public resources are appropriately allocated. Spatial planning products and decision-support tools developed for this purpose should enable partner organizations to achieve focus, coordination, and increased effectiveness in their investments and actions. Whereas conservation partnerships have historically created distinct planning tools, there is increasing interest for improved coordination, communication, and unifying biological datasets to improve the cohesiveness of regional management activities. We sought to inform spatial conservation planning efforts in the southeastern United States through the development of species distribution models for focal avian …
Reassessing Nitrogen Management For Maize Production In Mississippi, Camden Dawson Oglesby
Reassessing Nitrogen Management For Maize Production In Mississippi, Camden Dawson Oglesby
Theses and Dissertations
12 N treatments in 2020 and 11 in 2021 were replicated four times over four locations in a randomized complete block design. The first research tested the efficacy of CYG for Mississippi corn (Zea mays L.) production. The optimum N rates were calculated by fitting four models. Differences between the CYG rate and AONR were compared. AONR varied from 134 to 301 kg N ha–1 at different management levels. When we compared the AONR to the CYG rate, the CYG rate over-recommended N in 12 of the 14 possible comparisons. The second study compared different VIs, methods, and sensors at …
Disease Resistance And Productivity In Genetically Improved Loblolly Pine: Results From A Resistance Screening Trial And A Midrotation Comparison Of Genetic Improvement Levels, Stephen W. Goodfellow
Disease Resistance And Productivity In Genetically Improved Loblolly Pine: Results From A Resistance Screening Trial And A Midrotation Comparison Of Genetic Improvement Levels, Stephen W. Goodfellow
Theses and Dissertations
Decades of tree improvement has resulted in genetic gains in loblolly pine productivity, form, and resistance to fusiform rust. The goal of this study was to advance the understanding and applied use of genetic improvement by analyzing inter- and intra-provenances hybrids’ rust resistance and evaluating midrotation performance of varying levels of genetically improved stock types. The first study compares 16 seedlots at the USDA Resistance Screening Center and evaluates rust resistance of controlled-pollinated inter- and intra-provenances crosses, and openpollinated seedlots from three provenances: Western Gulf, Atlantic Coastal, and Interior Piedmont. Post inoculation, one Coastal OP seedlot was resistant and ten …
The Influence Of Taste Sensitivity To 6-N-Propylthiouracil (Prop) On Anthropometric Measurements, Body Composition, And Eating Behaviors Among Female College Students, Abeer Mohammedsharief Alardawi
The Influence Of Taste Sensitivity To 6-N-Propylthiouracil (Prop) On Anthropometric Measurements, Body Composition, And Eating Behaviors Among Female College Students, Abeer Mohammedsharief Alardawi
Theses and Dissertations
Taste is one of the crucial factors that contributes to shaping eating behaviors and is also one of the leading reasons that affects our preferences to like or dislike some foods that mainly have a bitter taste. Variation in bitter phenotype (tasters and non-tasters) could influence diet quality and in turn body weight, which overall influenced health outcomes. The objective of this study was to identify whether bitter taste phenotype status influences anthropometric measurements, body fat percentage, and eating behaviors (liking and intake) in female college students. In this cross-sectional study (n = 86), female college students aged 18 to …
Effects Of Soil Management Practices On Water Infiltration And Maize Yield To Improve Mississippi River Valley Alluvial Aquifer Sustainability, Jacob Rix
Theses and Dissertations
Surface sealing and hardpans in loam soils are problematic in the Mid-South U.S. because of intense tillage. Two experiments quantified the effects of soil management practices on infiltration and maize yield in loam soils. The first study measured the impacts of in-row subsoiling frequency × furrow irrigation frequency. In-row subsoiling significantly improved the infiltration of irrigation but not the infiltration of precipitation. In-row subsoiling with low irrigation frequency is optimal to achieve profitable maize yields while encouraging groundwater conservation. In the second study, six soil management treatments were imposed. For single-ring infiltrometer tests, infiltration rates were different between the two …
Evaluation Of Soybean [Glycine Max (L.) Merr.] Cultivar Response To Prophylactic Fungicide Application, Steven B. Stoker
Evaluation Of Soybean [Glycine Max (L.) Merr.] Cultivar Response To Prophylactic Fungicide Application, Steven B. Stoker
Theses and Dissertations
Soybean growers in the southern United States commonly deal with disease issues that can limit yield potential. As a result, growers have adopted prophylactic fungicide application targeted at specific growth stages as a standard management practice. However, yield responses to prophylactic applications have become sporadic over time. One major change that has occurred is adoption of cultivars with improved disease tolerances. Therefore, the objective of this research was to evaluate yield response of cultivars representing multiple genetic backgrounds to fungicides applied prophylactically at the R4 soybean growth stage. Experiments were conducted in 2020 and 2021 to determine the yield impact …
United We Stand? Investigating The Impact Of Unified Dues Agreements Between State And National Career & Technical Education (Cte) Professional Organizations On Cte Funding In The Southern States, Justin Ryan Hall
Theses and Dissertations
Career and Technical Education (CTE) teachers have long been active in lobbying the federal and state governments in support of CTE through their professional organizations, but these are not traditionally considered interest groups. This study sought to determine if the formal relationships—dues agreements—between the two largest national professional organizations, and their state affiliates had an impact on state funding for CTE. The impacts of professional organization unity, membership rates, and lobbying expenses on state-level CTE funding were analyzed through the lens of interest group theory, a subfield of political science. Within this population (N = 13), 1) a link …
Empathy And Cultural Involvement As Pathways To Resilience In The Face Of Adverse Childhood Experiences Among Native American Indians, Joy Rodriguez
Empathy And Cultural Involvement As Pathways To Resilience In The Face Of Adverse Childhood Experiences Among Native American Indians, Joy Rodriguez
Theses and Dissertations
The purpose of this research study was to examine two specific pathways to resilience for Native American Indians (NAIs) that have experienced at least one adverse childhood experience (ACE) and are seeking behavioral health services on the Choctaw Indian reservation: empathy and cultural involvement. The study is an examination of the effects of empathy and cultural involvement on the resilience of NAIs with one or more ACEs. The researcher also investigated the type of ACEs reported among adult NAIs seeking behavioral health services on the Mississippi Band of Choctaw Indian (MBCI) Reservation. Lastly, the association between ACEs and resilience was …
Dynamics Of Redox-Driven Molecular Processes In Local And Systemic Plant Immunity, Philip Berg
Dynamics Of Redox-Driven Molecular Processes In Local And Systemic Plant Immunity, Philip Berg
Theses and Dissertations
The work here presents two main parts. In the first part, chapters 1 – 3 focus on dynamical systems modeling in plant immunity, whereas chapters 4 – 6 describe contributions to computational modeling and analysis of proteomics and genomics data. Chapter 1 investigates dynamical and biochemical patterns of reversibly oxidized cysteines (RevOxCys) during effector-triggered immunity (ETI) in Arabidopsis, examines the regulatory patterns associated with Arabidopsis thimet oligopeptidase 1 and 2’s (TOP1 and TOP2), roles in the RevOxCys events during ETI, and analyzes the redox phenotype of the top1top2 mutant. The second chapter investigates the peptidome dynamics during ETI …
Towards Structured Planning And Learning At The State Fisheries Agency Scale, Caleb A. Aldridge
Towards Structured Planning And Learning At The State Fisheries Agency Scale, Caleb A. Aldridge
Theses and Dissertations
Inland recreational fisheries has grown philosophically and scientifically to consider economic and sociopolitical aspects (non-biological) in addition to the biological. However, integrating biological and non-biological aspects of inland fisheries has been challenging. Thus, an opportunity exists to develop approaches and tools which operationalize planning and decision-making processes which include biological and non-biological aspects of a fishery. This dissertation expands the idea that a core set of goals and objectives is shared among and within inland fisheries agencies; that many routine operations of inland fisheries managers can be regimented or standardized; and the novel concept that current information and operations can …
The Emerging Value Of The Viroid Model In Understanding Plant Responses To Foreign Rnas, Junfei Ma
The Emerging Value Of The Viroid Model In Understanding Plant Responses To Foreign Rnas, Junfei Ma
Theses and Dissertations
RNAs play essential roles in various biological processes. Mounting evidence has demonstrated that RNA subcellular localization and intercellular trafficking govern their functions in coordinating plant growth at the organismal level. Beyond that, plants constantly encounter foreign RNAs (i.e., RNAs from pathogens including viruses and viroids). The subcellular localizations of RNAs are crucial for their function. While numerous types of RNAs (i.e., mRNAs, small RNAs, rRNAs, tRNAs, and long noncoding RNAs) have been found to traffic in a non-cell-autonomous fashion within plants, the underlying regulatory mechanism remains unclear. Viroids are single-stranded circular noncoding RNAs, which entirely rely …
Evaluating The Impacts Of Waterlogging Stress On Cowpea (Vigna Unguiculata L.) Growth Traits And Physiological Performance, Omolayo Joshua Olorunwa
Evaluating The Impacts Of Waterlogging Stress On Cowpea (Vigna Unguiculata L.) Growth Traits And Physiological Performance, Omolayo Joshua Olorunwa
Theses and Dissertations
The progressive increase in the global population and the rapidly changing climate have put unprecedented pressure on crop production. Cowpea is one of the world’s most important leguminous crops, contributing to food security and environmental sustainability. However, cowpea productivity is limited due to waterlogging stress. The main objective of this study was to explore physiological and biochemical mechanisms to understand how cowpea genotypes respond to waterlogging stress. Four studies were conducted in controlled and field conditions to achieve these objectives. Study 1 characterized the waterlogging tolerance of 30 cowpea genotypes in a controlled environment using 24 morphophysiological parameters with waterlogging …
Molecular Basis Of Viroid Rna-Templated Transcription, Shachinthaka D. Dissanayaka Mudiyanselage
Molecular Basis Of Viroid Rna-Templated Transcription, Shachinthaka D. Dissanayaka Mudiyanselage
Theses and Dissertations
Transcription is a fundamental process catalyzed by DNA-dependent RNA polymerases (DdRPs). Interestingly, some DdRPs can use both DNA and RNA as templates for transcription. This RNA-dependent RNA polymerase (RdRP) activity of DdRPs is used by RNA-based pathogens such as viroids and hepatitis delta virus for replication. In addition, RdRP activity of DdRPs widely occurs in various organisms to regulate gene transcription. Despite the importance of this intrinsic RdRP activity of DdRPs, associated factors and mechanisms are in their infancy stage. We employed potato spindle tuber viroid (PSTVd) as a model to study RNA-templated transcription. Here, we present evidence showing that …
Non-Traditional Adjuvants And Methods For Applying Root-Promoting Compounds In Commercial Cutting Propagation, Anthony Turner Bowden
Non-Traditional Adjuvants And Methods For Applying Root-Promoting Compounds In Commercial Cutting Propagation, Anthony Turner Bowden
Theses and Dissertations
The nursery/greenhouse industry is innovative. There have been several reports in the popular literature of non-traditional adjuvants used in plant propagation; however, these claims have never been evaluated in a scientific setting. Five studies, conducted at the South Mississippi Branch Experiment Station in Poplarville in 2019 and 2021 determined the impact that adding honey to water-soluble auxin solutions and surfactants to foliar applied auxin solutions on physiological responses associated with adventitious root formation. Treatments in studies presented in chapters two and three included three honey sources (multiflora, local, and Manuka). Honey-infused water-soluble auxin solutions affected plant species but not in …