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Life Sciences

Louisiana State University

Theses/Dissertations

2003

Rice

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Studies On Rice Transformation And The Use Of Transformed Plants [Electronic Resource], Qiming Shao Jan 2003

Studies On Rice Transformation And The Use Of Transformed Plants [Electronic Resource], Qiming Shao

LSU Doctoral Dissertations

This research was conducted to enhance utilization of the Liberty herbicide resistance transgene in rice. Non-lethal methods to determine the sensitivity of transgenic rice plants to hygromycin B and Liberty were developed, tested and used in this research. Four homozygous transformed plants were selected to make reciprocal crosses with their non-transformed parent cultivars Taipei 309 and Nipponbare. Their resistances to Liberty and hygromycin B were controlled by the closely linked single dominant genes bar and hpt. Some non-resistant phenotypes in the F2 populations were due to gene silencing. The bar gene in some of these plants were allelic and some …


Resistant Rice Starch Development, Siow Ying Tan Jan 2003

Resistant Rice Starch Development, Siow Ying Tan

LSU Master's Theses

This study showed that enzyme treatments were effective in producing resistant starch from rice flour and starch. Rice starch produced higher resistant starch yields than rice flour. The lower resistant starch yields in rice flour could be attributed to the presence of lipids and proteins which were competitively binding to amylose, resulting in decreased recrystallization of amylose. The gelatinization type and incubation periods were not as influential as enzyme treatments in the resistant starch formation. The rapid visco amylograph (RVA) analysis indicated that the gelatinized samples have minimal pasting characteristics since they had been gelatinized prior to enzyme treatments. Pasting …