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Life Sciences

John Carroll University

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16S–23S ITS

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Roholtiella Volcanica Sp. Nov., A New Species Of Cyanobacteria From Kamchatkan Volcanic Soils, Lira A. Gaysina, Jeffrey R. Johansen, Aniket Saraf, Razeda Z. Allaguvatova, Sagarika Paul, Prashant Singh Jan 2022

Roholtiella Volcanica Sp. Nov., A New Species Of Cyanobacteria From Kamchatkan Volcanic Soils, Lira A. Gaysina, Jeffrey R. Johansen, Aniket Saraf, Razeda Z. Allaguvatova, Sagarika Paul, Prashant Singh

2022 Faculty Bibliography

During a study of biodiversity of cyanobacteria in Gorely volcano soils (Kamchatka Peninsula), a strain of heterocytous, a false branching cyanobacterium with gradually tapered filaments, was isolated. Prominent features of the strain were purplish-grey trichomes and firm, distinct multilayered sheaths. Based on the results obtained from the morphological, ecological, and phylogenetic analysis using the 16S rRNA and 16S–23S ITS region, 16S–23S ITS secondary structure analysis, comparison of flanking regions of BoxB and V3 helices, and the p-distance between the 16S–23S ITS region, we describe our strain K7 as a novel species of the genus Roholtiella with the name Roholtiella volcanica …


Two New Oculatella (Oculatellaceae, Cyanobacteria) Species In Soil Crusts From Tropical Semi–Arid Uplands Of México, Itzel Becerra–Absalón, Jeffrey R. Johansen, Karina Osorio–Santos, Gustavo Montejavo Jan 2020

Two New Oculatella (Oculatellaceae, Cyanobacteria) Species In Soil Crusts From Tropical Semi–Arid Uplands Of México, Itzel Becerra–Absalón, Jeffrey R. Johansen, Karina Osorio–Santos, Gustavo Montejavo

2020 Faculty Bibliography

Two isolates of Oculatella from biological soil crusts in the arid and semi–arid tropical upland region of México were characterized using a polyphasic approach which included morphology, ecology, 16S rRNA and 16S–23S ITS phylogenetic analysis, percent identity of 16S rRNA gene sequence, percent dissimilarity of 16S–23S ITS sequence, and secondary structure of conserved ITS domains. The two species shared the generic synapomorphy of a reddish granule at the tip of mature apical cells, and possessed a single ribosomal

operon with both tRNA genes (tRNAIle and tRNAAla) based upon our analysis and the analysis of the other spe- cies in the …


Lagosinema Tenuis Gen. Et Sp. Nov. (Prochlorotrichaceae, Cyanobacteria): A New Brackish Water Genus From Tropical Africa, Sandra C. Akagha, Jeffrey R. Johansen, Dike I. Nwankwo, Kedong Yin Jan 2019

Lagosinema Tenuis Gen. Et Sp. Nov. (Prochlorotrichaceae, Cyanobacteria): A New Brackish Water Genus From Tropical Africa, Sandra C. Akagha, Jeffrey R. Johansen, Dike I. Nwankwo, Kedong Yin

2019 Faculty Bibliography

A novel filamentous, nonheterocytous cyanobacterium was isolated from Lagos Lagoon, Nigeria. The isolate was <3.0 μm wide, untapered, with small rounded polar bodies (aerotopes or cyanophycin granules) visible at the crosswalls, and consequently fit the morphological description of Limnothrix planctonica. Although morphologically inseparable from that species, it was molecularly distant from that taxon, with genetic identities between the two taxa ranging 90.73–92.49%, a degree of separation typical of different genera. Both taxa, as well as Limnothrix rosea, are phylogenetically in the Prochlorotrichaceae, distant from the type species of Limnothrix, L. redekei, which is in the Pseudanabaenaceae. The isolate is herein described as Lagosinema tenuis gen. et sp. nov.