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Life Sciences

James Madison University

Theses/Dissertations

2017

Biotization

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Impact Of Burkholderia Phytofirmans Strain Psjn On The Ex Vitro Acclimatization And In Vitro Propagation Of Tissue Cultured Dionaea Muscipula (Venus Fly Trap), Peter L. Riley May 2017

Impact Of Burkholderia Phytofirmans Strain Psjn On The Ex Vitro Acclimatization And In Vitro Propagation Of Tissue Cultured Dionaea Muscipula (Venus Fly Trap), Peter L. Riley

Masters Theses, 2010-2019

Dionaea muscipula, Venus fly trap, is an endangered plant that is propagated primarily through tissue culture. Tissue cultured plants must be acclimatized to their new environments when removed from their test tubes and the ensuing stress can result in plant mortality. A plant growth promoting rhizobacterium, Burkholderia phytofirmans strain PsJN, has been utilized in biotic hardening of tissue cultured plants and in the amelioration of stresses, particularly drought stress in potted plants. We investigated whether this bacterium could improve either propagation or acclimatization of tissue cultured Venus fly traps. Standardized inoculations were performed by adjusting cell density of inoculant …