Digital Commons Network^™

Estimating Daily Growth Of Age-0 Channel Catfish In A Free-Flowing, Commercially Exploited Midwestern River, Kaleb B. Wood

Masters Theses

The larval stage in fishes is an important stage in the life history, as their survival dictates the ability of the population to sustain itself as an adult population. While there is a plethora of information available for juvenile and adult life stages of Channel Catfish (Ictalurus punctatus), very little is understood about their larval life stage. This study sought to determine overall density, growth rates, and size structure of cohorts of age-0 Channel Catfish during a study year on the Wabash River. In 2018, I sampled age-0 Channel Catfish using a benthic trawl from May to September. …

Acute And Chronic Impacts Of A Biofilm, Activated Sludge, And Ultraviolet Radiation Treatment On The Fathead Minnow, Pimephales Promelas, Julia Fitch

Masters Theses

Wastewater treatment plants (WWTPs) are a point source of pollution that have potentially damaging effects on downstream fish populations, including reduced growth and survival and reproductive complications. To examine these possible effects, fathead minnows were exposed to effluent of three wastewater treatment processes at the Charleston Wastewater Treatment Plant located in Charleston, Illinois. These three treatments included two secondary treatments, a biofilm treatment and an activated sludge treatment, and one tertiary U.V. treatment. Two trials took place during this project: an acute, two-week exposure trial, and a chronic 119 day exposure trial. Upon completion of each trial, fish were assessed …

Population Demographics Of Channel Catfish In The Lower Wabash River, Valerie J. Thompson

Masters Theses

Inland fisheries are experiencing increasing demands due to anthropogenic stressors, especially within large river systems that support both recreational and commercial fisheries. Riverine species are sensitive to habitat degradation, species introductions, and exploitation, so continued monitoring and management of these communities are vital to maintain abundance and diversity within these systems. The North American catfish family, Ictaluridae, supports both recreational and commercial fisheries throughout the United States. Channel Catfish Ictalurus punctatus are heavily studied and managed compared to other sport catfishes in this family because of their economic and ecological significance. One important Channel Catfish fishery exists in the lower …

Exploring A Medicinal Strain Of White-Rot Fungus For Biofuel Applications, Kortney L. Lucius

Masters Theses

The major limitation to producing biofuels from lignocellulosic biomass, such as miscanthus straw, is the difficulty in accessing fermentable carbohydrates encased in lignin. Traditional methods for separating lignin are economically and environmentally costly. Biological pretreatment using white-rot fungi, such as Trametes versicolor, has been demonstrated to potentially reduce the need for harsh chemical and physical pretreatment methods. Despite these efforts, the process of converting lignocellulose to ethanol remains financially challenging. To explore a potential co-product for the process, this study evaluated the efficacy of T. versicolor strain CM-101, known for producing the valuable medicinal macromolecule polysaccharide-K (PSK), as a …

Using Community Science To Monitor Eastern Box Turtles And Engage Public In Conservation, Sarah Zoppa

Masters Theses

The eastern box turtle (Terrapene carolina carolina; EBT) is a charismatic species with populations in decline due to habitat loss, disease, and other anthropogenic factors. However, due to their cryptic nature, studying an EBT population can be a difficult and intensive process. To investigate EBT populations in Coles County, Illinois, we used a combination of community science and computer-based pattern analysis to develop an approach to tracking individual turtles. Citizen volunteers submitted photos of encountered eastern box turtle carapaces for shell pattern analysis along with GPS coordinates of the encounter. We have received 220 submissions from 47 community volunteers. …

Exploring Anterior Cruciate Ligament Tears And Athletic Identity As Predictors Of Disordered Eating Behaviors In Female Collegiate Soccer Players, Niondina M. Nyström

Masters Theses

Research has consistently established the relationship between injury occurrence and subsequent mental health concerns within the athletic population (Brown et al., 2014; NCAA, 2021). Specifically, negative mental health outcomes such as anxiety, depression, and social isolation have followed the occurrence of an anterior cruciate ligament (ACL) tear (McGinley et al., 2022; Töyrä & Bergström, 2017). Similarly, because of high levels of athletic identity (AI), the inability to participate in a sport due to injury has been associated with disordered eating (DE) (Gusfa et al., 2022). Despite this, no studies have examined disordered eating and the role of athletic identity after …

Effects Of Prescribed Fire On Planted Oak Growth And Survival In Restored Savannas, Allison Earl

Masters Theses

Most oak savannas in the Midwestern United States have been lost to agriculture and habitat degradation. Because of their rarity and ability to support high plant and animal diversity, savannas are often a target for restoration. Oak savanna restoration frequently relies on direct planting of oak seedlings to establish the necessary tree canopy. Returning fire to the system is critical to the herbaceous component of the savanna, but managers risk damaging or killing trees if burning is introduced too soon. I studied growth and physiological responses of three oak species (Quercus alba, Q. macrocarpa, and Q. velutina …

Wolbachia Infection In Gall Associated Insect Communities In Illinois And Indiana, Jakeb Watts

Masters Theses

Wolbachia is a maternally inherited, gram-negative bacterium and has been found to be associated with up to 60% of all insects, frequently resulting in male-killing, feminization, and cytoplasmic incompatibility in the hosts. Gall wasps and other gall-forming insects induce on various host plants abnormal tissue growths, or plant galls, with distinct morphology. They are considered as obligatory parasites to their host plant. The presence of Wolbachia infection in gall wasp populations in North America has not been extensively documented. Given the strong influence on host reproduction, not knowing Wolbachia infection in insect populations prevents the understanding of how populations are …

Comparison Of Bigheaded Carp Reproduction In Tributaries Of The Illinois And Wabash Rivers, David J. Yff

Masters Theses

Understanding environmental conditions that support bigheaded carp (Hypophthalmichthys sp.) reproduction in tributary rivers provides key predictions of their invasion throughout the United States. Past research, primarily in larger order rivers, identified factors such as discharge, temperature, and turbidity as main environmental drivers of bigheaded carp reproduction. I used the variable hydrologic conditions between the Illinois and Wabash Rivers to compare environmental conditions between tributaries of each basin, determine which conditions influence bigheaded carp reproduction in tributaries, and predict tributaries likely to support bigheaded carp reproduction. Three tributaries of the Illinois and Wabash Rivers were sampled for ichthyoplankton from …

Beetle Diversity In Temperate Deciduous Forests With Different Management History In Central Illinois, Syue Ru Chien

Masters Theses

No abstract provided.

Development Of Phase Change Thermal Storage Medium: Cooking With More Power And Versatility, Martin Osei

Masters Theses

With an Insulated Solar Electric Cooker (ISEC), a 100 W solar panel directly cooks food while providing 5W off-grid electricity access; but it cooks slowly. Storing the day’s energy with phase change thermal storage allows ISEC to cook more rapidly as well as cook after sunset. The ISEC is made by sticking a 3ohm resistive to an aluminum pot (inner phase change assembly PCA) where another cooking pot fits. The resistive heater is surrounded by a bigger pot (outer PCA) that contains the PCM and is tightly sealed. The solar panel is connected to the resistive heater with a thermal …

Low-Head Dam Removal Increases Functional Diversity Of Stream Fish Assemblages, Adam Christopher Jones

Masters Theses

Despite the growing number of dam removals to date, very few have been studied to understand their impacts on stream fish communities. Despite being the most common type of dam in the U.S., an even smaller proportion of studies focus on the impacts of low-head dam removals, instead, focusing on the impacts of removal of larger dams. In this study, two previously impounded Illinois rivers were monitored to assess the impacts of low-head dam removal on the functional assemblage of stream fishes. This was accomplished by aggregating fishes into habitat and reproductive guilds, relating community changes to habitat, environmental metrics, …

Deciphering The Role Of Mitochondrial Physiology And Thermal Acclimation In Shaping Whole Organismal Performance Of An Invasive Forest Pest, Essa Alrashdi

Masters Theses

Environmental factors such as temperature are substantial determinants of the spongy moth, L. dispar, distribution, reproduction, and growth. Accumulating energy reserves at the larval stage is particularly important to L. dispar, since the larvae metamorphose into a fully-grown, non-feeding adult. As non-feeding adults, the energy balance of the pupae must be adequate, to ensure enough energy reserves for adult dispersal, egg maturation and overall persistence of the species in the region. At this stage, environmental temperature also determines daily metabolic demands, and the overall cost of sustaining ecologically relevant activities. Various hypotheses describing a mismatched growth and metabolic …

Cloning Of Root-Specific Promoters In Helianthus Annuus And Populus Trichocarpa, Jacob Murphy

Masters Theses

Tissue-specific promoters are a valuable tool in plant biotechnology because they allow for precise spatial control of transgene expression. To identify root-specific promoters in sunflower (Helianthus annuus), sunflower RNA sequence (RNA-seq) data were screened in silico by Pavlidis Template Matching (PTM) with a root-specific template. Eight root-specific genes were identified in sunflower. The 2.5kbp promoter regions of six of these root-specific sunflower genes (HaTOR1L3, HaGOXL3A, HaGOXL3B, HaAPA1, HaPOE1, and HaMOP10) were successfully cloned as well as the 3kbp promoter regions of four root-specific genes previously identified in poplar (Populus trichocarpa …

Differences In The Growth And Bioenergetics Between Centrarchids In A Midwestern Power Plant Lake, Kyle Joseph Rempe

Masters Theses

Black Crappie Pomoxis nigromaculatus and Bluegill Lepomis macrochirus are popular sportfishes that co-occur in similar bodies of water throughout most of the Central and Eastern United States but have distinct thermal physiologies. L. macrochirus is often found in warmer regions of freshwater reservoirs, where P. nigromaculatus tends to exploit cooler regions, especially in power plant cooling reservoirs. The coal-fired Coffeen Power Station ceased energy production in October 2019, providing an ideal scenario to study the organismal effects of the sudden shift in thermal regime. Previous studies showed that P. nigromaculatus inhabited the less thermally-impacted areas of Coffeen Lake, whereas L. …

Xylan-Derived Carbon Nutrient Acquisition By Caulobacter Crescentus, Rhoda Ekuama Inkoom

Masters Theses

Bacterial decomposition of xylan and uptake of xylan-derived carbon compounds require a combination of enzymes, proteins, and complex molecular mechanisms. Bacteria have evolved molecular mechanisms for its utilization depending on its environment and source of xylan. Caulobacter crescentus, a Gram-negative bacterium which lives in an aquatic oligotrophic environment, exhibits asymmetric division which is an adaptive feature for its nutrient poor habitat. Although aromatic hydrocarbon and cellulose-derived mono-, di- and oligosaccharide utilization has been reported in literature, its xylan-derived carbon compound utilization is yet to be characterized. In this study, C. crescentus utilizes xylan-derived monomers arabinose, xylose and acetate as …

Genetic Relatedness Can Alter The Strength Of Plant-Soil Feedbacks, Kelly M. Clark

Masters Theses

Intraspecific variation may play a key role in understanding the relationships between plants and their interactions with soil microbial communities. The effects that soil-microbes have on individuals can venerate variation across individuals in their responsiveness. I explored how relatedness alters plant-soil feedbacks in established Solidago altissima clones grown in a common garden. Seedlings of known parentage were inoculated with soils from the maternal, paternal, or unrelated clones and compared to autoclaved control inocula. I found that the soil inocula generated from S. altissima had an overall negative effect of seedling biomass. Furthermore, seedlings inoculated with maternal or paternal soils experienced …

The Gene Expression Patterns Of Phanerochaete Chrysosporium And Trametes Versicolor On Diverse Lignocellulosic Feedstocks, Noor Osama Alabbasi

Masters Theses

Plant biomass is considered to be an important future starting material for fuels and chemicals, thereby decreasing our reliance on fossil fuels. While direct combustion continues to be a source of fuel, the generation of liquid fuels from plant biomass for transportation has proven to be challenging. Among the limiting factors for conversion of this material to biofuels is the recalcitrant nature of lignocellulose, the primary component of non-starch plant biomass. A strategy to overcome this dilemma is to directly or indirectly use the enzymes from white-rot fungi, which have evolved a unique ability to deconstruct lignocellulose. However, the biochemical …

Genome-Wide Characterization Of Lateral Organ Boundaries Domain Genes In Sunflower (Helianthus Annuus L.), Saleh M. Aljurbua

Masters Theses

Lateral Organ Boundaries (LBD) gene family encodes transcription factors found only in plants with essential roles in growth, development, and response to biotic and abiotic factors. The sunflower (Helianthus annuus L., Asteraceae family) LBD genes were studied using different in silico and phylogenetic approaches including four major steps: gene identification, phylogenic analysis, structural analysis, and expression analysis. Sixty-four sunflower LBD genes (HaLBDs) were identified using query search of all Arabidopsis LBD genes against sunflower genome (version r1.2) followed by LBD domain sequence verification. A phylogenetic tree was reconstructed using MEGA-X program. The identified genes were phylogenetically classified into two main …

Immediate Response Of Black Basses To Dam Removal Is Determined By Habitat Preference, Reuben Frey

Masters Theses

Dams affect the abundance of fish species in lotic systems by altering flow regime and available physical habitat. Removal of dams may mitigate these effects and generate a change in species abundance and fish community structure. Understanding the effects of dam removal on gamefish species is essential in making management decisions. I investigated how populations of Smallmouth Bass (Micropterus dolomieu), Spotted Bass (M. punctulatus), and Largemouth Bass (M. salmoides) were affected by the presence and removal of two low-head dams in a Midwestern river system. I used data collected during fall and spring from …

Development Of Fluorescence Microscopy Approaches To Study Subcellular Protein Transport And Enzymatic Activity, Anchal Singh

Masters Theses

Understanding the subcellular localization of proteins and their activity is important in understanding their normal function in eukaryotic cells. Fluorescence cellular imaging techniques can selectively and sensitively visualize subcellular biochemistry. Using this approach, two different methods were employed in this thesis. The first focused on studying protein import into peroxisome and the other on monitoring the activity of an endoplasmic reticulum (ER)-localized enzyme, human carboxylesterase 1 (CES1).

Peroxisomes are mainly known as the center for long chain fatty acid b-oxidation as well as the production and detoxification of hydrogen peroxide. Proteins which are needed in the peroxisomes are encoded in …

Effects Of Emerging Contaminants On Centrarchidae And Catostomidae In Midwestern Rivers: A Multiple Biomarker Approach, Camden Garret Nix

Masters Theses

Natural habitats of fish are gradually declining due to land use and pollution caused by industrial wastes, intensive agriculture and contamination from sewage systems. The United States has 14,780 wastewater treatment facilities that discharge effluent into navigable waters, and in some cases these discharge waters represent a considerable proportion of the water system flow. These activities make it critical to discern the effects of pollutants that are entering our waterways at a consistent rate, such as 17β-estradiol (E2) and nickel, on aquatic organisms. To determine the effects of 17β-estradiol on endocrine disruption in fish, Bluegill Sunfish (Lepomis macrochirus) …

Effects Of Wastewater Effluent On Fish Fin Morphology Of Larval Fathead Minnows (Pimephales Promelas) And Swimming Performance Of Smallmouth Buffalo (Ictiobus Bubalus), Seth Michael Bogue

Masters Theses

The Sangamon River flows across central Illinois and is mostly free flowing except in the city of Decatur where it is impounded to form Lake Decatur. The Sanitary District of Decatur (SDD) is located approximately three miles downstream of the Lake Decatur dam. For a significant portion of the year, the stretch of the Sangamon River downstream of SDD is dominated by effluent due to limited discharge from the Lake Decatur dam. This has resulted in heavy nutrient loading, most notably phosphorus, and elevated levels of nickel and other compounds in the downstream section of the river. Smallmouth buffalo ( …

The Relationship Between Relative Muscular Strength And Joint Mobility In Firefighters, Samuel C. Nozicka

Masters Theses

Firefighters job requirements consist of running upstairs, climbing ladders, ceiling breach and pull, carrying equipment, forcible entry, dragging hoses, raising ladders, and rescue of patrons all while wearing heavy protective equipment that limits their mobility (Park et al., 2015). The purpose of this study was to examine the relationship between relative strength and mobility within the population of firefighters. The subjects were volunteers consisting of twelve male firefighter ranging in age from 25-52 with the mean age of 37.7 ± 7.7 years. Leighton Flexometer was used to measure joint range of motion (ROM) in different active movement patterns (Leighton, 1966). …

Flexibility: The Forgotten Component Of Fitness: Eccentric Exercise Vs. Static Stretching To Improve Flexibility, Zach Cox

Masters Theses

The purpose of this research is to assess the effects of eccentric exercise as well as static stretching on flexibility following a six-week training intervention. It also compared the effects to those of a static stretching program. The hypothesis is that eccentric training will show larger enhancements in flexibility in sagittal plane movements of the hips and knees in comparison to a static stretching program. Training sessions were done three days per week and had a 24-hour rest period minimum between them. The exercises for the eccentric group were as follows: standing hip extension, standing split, straight leg lowering, and …

Chemical Diversity And Antimicrobial Properties Of Spike-Rush Species And Related Genera (Genus Eleocharis, Family Cyperaceae), Yiru Zhang

Masters Theses

The focus of this research was to explore the chemical diversity and antimicrobial properties of Eleocharis dulcis. By using methanol as a solvent to extract phytochemicals, qualitative comparisons were made between HPLC chromatograms from Eleocharis species and other genera. Meanwhile, this study also examined the antimicrobial properties of phytochemicals extracted from various tissues of Eleocharis dulcis in two different solvents (water or methanol) against two Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli) and two Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) using a disc diffusion assay. The results suggested that the extracts had limited antimicrobial properties at up to 100 …

A Cloning Strategy For Expression Of Fungal Hemicellulases In Plant Systems, Amer Alrudayan

Masters Theses

Lignocellulosic biomass is notoriously difficult to deconstruct into lignin, hemicellulose, and cellulose fractions without the use of biochemical, chemical, and/or mechanical pretreatments. These pretreatment strategies add operational cost and in some cases produce hazardous waste that poses health and safety risks. Direct biological pretreatment from biomass-degrading bacteria and fungi may significantly reduce the costs and risks associated with these traditional strategies due to the innate ability of these organisms to modify plant cell walls. An additional measure that could further reduce the expenses associated with lignocellulosic biomass fractionation is genetic modification of the lignocellulosic feedstocks, which can be accomplished by …

Effects Of Insect Opportunists On A Four-Level Trophobiotic System Involving Nectar-Producing Galls Of The Cynipid Wasp Disholcaspis Quercusmamma (Hymenoptera: Cynipidae), Stephanie L. Smith

Masters Theses

The induction of plant galls is considered an adaptive life history trait found in many insect groups. The formation of galls provides several advantages to the gall maker, such as enhanced nutrition, favorable microclimate, and protection from natural enemies, including parasitoids, inquilines, and predators. Order Hymenoptera has many gall-making species, belonging to the gall wasp family Cynipidae. As an extended phenotype of the gall makers, some galls exhibit very sophisticated adaptive mechanisms involving multilevel species interactions. In particular, the oak galls of the Cynipid species Disholcaspis quercusmamma, found in much of Illinois, produce a palatable, sugary nectar-like secretion, attracting …

Transcriptome Analysis Of Neuro-2a Cells Treated With Asiatic And Madecassic Acid, Fatimah M. Alqam

Masters Theses

Traditional herbal medicine is ingrained as a source of therapeutic compounds to medicate various diseases. The family Araliaceae (Ginseng family) is rich in traditional medicine species, such as Centella asiatica (CA). For many centuries, CA has been used by the indigenous Indian and Chinese in Ayurvedic and traditional medicine, respectively, to improve intelligence, learning, memory, and cognitive performance. Previous studies on cell culture and animal models supported the beneficial effects of CA on the nervous system. However, the exact composition of CA extract and its molecular mechanism that leads to neuroprotection is still unclear. We examined the effect of asiatic …

Seasonal Temperature Changes In Three Midwestern Wastewater Streams And The Effects On Fish Assemblages And Physiology, Ryan William Sparks

Masters Theses

Midwestern United States undergo extreme temperature and precipitation fluctuations throughout the year, which leads to changing habitats within Illinois river systems. Wastewater treatment facilities have long been used to treat sewage and reduce the organic products in sewage before releasing it into aquatic environments. Wastewater treatment facilities must maintain optimal conditions, especially temperature, for reproduction and growth of facilitated bacteria that break down organic materials in wastewater. The objectives for this study were to determine the thermal effects of wastewater treatment facilities on receiving waters; and secondly, to investigate the influence of these wastewater facilities on fish abundance and assemblages. …