Open Access. Powered by Scholars. Published by Universities.®
Dominican University of California
Dissertations, Masters Theses, Capstones, and Culminating Projects
- Keyword
-
- APP (1)
- Alendronate (1)
- Alzheimer's (1)
- Amyloid (1)
- Amyloid-β Precursor Protein (1)
-
- Bone Loss (1)
- DNA (1)
- DNA Mutations (1)
- DNA Testing (1)
- High-Performance Liquid-Chromatography (1)
- Huntington's Neural Stem Cell Model (1)
- Late onset alzheimer's disease (1)
- MS (1)
- Macular degeneration (1)
- Metabolites (1)
- Mitochondrial Oxidative Stress (1)
- Muscular Dystrophy (1)
- Mutation (1)
- RNA (1)
- Retinal stem cells (1)
- Signaling intermediates (1)
- Single Cell Gene Expression (1)
Articles 1 - 8 of 8
Full-Text Articles in Entire DC Network
Neural Stem Cells As A Model To Study Huntington’S Disease, Rawan Bakhsh
Neural Stem Cells As A Model To Study Huntington’S Disease, Rawan Bakhsh
Dissertations, Masters Theses, Capstones, and Culminating Projects
Huntington’s disease (HD) is a heritable neurodegenerative disorder that affects muscle coordination and diminishes cognitive abilities, by affecting the medium spiny neurons in the brain. In HD patients, neurons are damaged and destroyed because of the toxicity of the mutant Huntington protein (mHtt). The mechanism of how mHtt protein affects the neurons is unknown. In this study we explored the effects of mHtt expression by looking at changes in huntingtin localization, changes in the expression and co-localization of related proteins and differences in cell morphology. We examine how this expression affects the cytoskeletal structures using neural stem cells Q7 (wild …
Effects Of Heregulin On Muscle: A Biochemical And Histological Analysis, Nicole Justine Moss
Effects Of Heregulin On Muscle: A Biochemical And Histological Analysis, Nicole Justine Moss
Dissertations, Masters Theses, Capstones, and Culminating Projects
Duchenne Muscular Dystrophy, the most common inherited X-linked genetic disease affecting 1 in 5000 boys, results from a dysfunctional dystrophin protein encoded by the DMD gene. Dystrophin interacts with protein complexes linking the extracellular matrix to the cytoskeleton of muscle fibers. Without dystrophin functioning properly, sarcolemmal membrane stabilization is compromised during the mechanical rigors involved with muscle contraction leading to progressive muscular dystrophy. Heregulin/neuregulin-1 (HRG), a member of the epidermal growth factor family has been shown to induce neuromuscular junction (NMJ) gene expression in vitro and improve skeletal muscle function in dystrophic mice. However, it is unclear if the HRG …
Consequences Of Endogenous Mitochondrial Oxidative Stress In The Heart, Kristin Alisha Koenig
Consequences Of Endogenous Mitochondrial Oxidative Stress In The Heart, Kristin Alisha Koenig
Dissertations, Masters Theses, Capstones, and Culminating Projects
For many years, it has been proposed that oxidative stress within the mitochondria causes mutations to the mitochondrial genome, resulting in changes in copy number per single cell. Ultimately, this compensatory increase in mtDNA, coupled with an increase in mutations, has been suggested to play a major role in the pathobiology of aging. However, evidence for this phenomenon is somewhat controversial. Importantly, oxidative stress can vary between individual cells; therefore, the overall goal of this project is to determine if oxidative stress causes both an altered copy number for mtDNA in singlecardiomyocytes and develop a methodology for sequencing mtDNA from …
Microrna Expression Signature Of Retinal Pigmented Epithelial Cells Associated With The Phenotype Of Age-Related Macular Degeneration, Mark Anthony Gutierrez
Microrna Expression Signature Of Retinal Pigmented Epithelial Cells Associated With The Phenotype Of Age-Related Macular Degeneration, Mark Anthony Gutierrez
Dissertations, Masters Theses, Capstones, and Culminating Projects
Age-related macular degeneration is a debilitating condition that manifests as a loss of the central portion of an individual’s field of vision, affecting 1 in 3 people over the age of 80. With regards to the retina, the organ primarily responsible for an individual’s sense of vision, this condition is associated with the degeneration of the central point in the retina, known as the macula, which contains the highest concentration of light-sensitive photoreceptors. It is currently known that the human retina exhibits a gradual decrease in protective detoxifying factors such as glutathione-S-transferase-1 and catalase as well as increased lipid peroxidation, …
Apolipoprotein E4 And Sirt1 Interaction In Alzheimer’S Disease, Brittany Ann Philpot
Apolipoprotein E4 And Sirt1 Interaction In Alzheimer’S Disease, Brittany Ann Philpot
Dissertations, Masters Theses, Capstones, and Culminating Projects
Alzheimer’s disease (AD) is a neurodegenerative disorder whose exact cause(s) are still unknown. Εpsilon 4, an allele of apolipoprotein E (ApoE4), is currently the most important risk factor for Alzheimer’s disease. Individuals that inherit two copies of the ApoE ε4 allele have an approximately ten to thirty times increased risk of developing AD in comparison to persons not carrying this allele. This association may relate to ApoE4’s susceptibility to proteolysis and neurotoxicity. Amyloid precursor protein (APP) is involved with neurite extension and neurite retraction, and has been shown to function as a molecular switch with two separate cleavage patterns. Cleavage …
Alendronate Alters Single Cell Gene Expression Of Cortical Osteoblast Lineage Cells During Bone Loss, Ryan Jakob Murphy
Alendronate Alters Single Cell Gene Expression Of Cortical Osteoblast Lineage Cells During Bone Loss, Ryan Jakob Murphy
Dissertations, Masters Theses, Capstones, and Culminating Projects
The mineralized matrix of bone makes it difficult to examine specific populations of cells which are integral to the tissue using traditional molecular methods. For this study we examined single cell cortical osteoblasts derived from the femurs of C57BL/6J mice. After isolating single cells from bone, we were able to individually analyze their gene expression profiles using the quantitative polymerase chain reaction (qPCR). The mice used for the study were divided into 4 treatment groups, including ovariectomized mice (OVX) and sham surgical controls (SHAM), treated with or without the anti-resorptive bisphosphonate drug Alendronate, an effective FDA approved therapeutic for slowing …
Application Of High-Performance Liquid-Chromatography Mass-Spectrometry Platform To Study Metabolism And Epigenetic Control Of Metabolism, Kylie Patricia Mitchell
Application Of High-Performance Liquid-Chromatography Mass-Spectrometry Platform To Study Metabolism And Epigenetic Control Of Metabolism, Kylie Patricia Mitchell
Dissertations, Masters Theses, Capstones, and Culminating Projects
Naturally occurring small molecules (metabolites, signaling intermediates) are a critical component of the information flow in biology, along with DNA, RNA, and proteins. Metabolomics is an analytical approach that seeks to comprehensively analyze naturally occurring small molecules and quantify their dynamic changes in biological systems. In recent years metabolomics has begun to provide understanding of the metabolic basis of different diseases, such as heart disease, cancer, and diabetes. Our lab built a High-Performance Liquid-Chromatography Mass-Spectrometry (HPLC-MS) based metabolomics platform to analyze metabolites from mammalian cells, spent cellular media, and model organisms such as C. elegans. We used C. elegans to …
Evaluation Of Select Publicly Available In Silico Methods For Predicting Functional Effects Of Missense Mutations In The Galns Gene, Kathryn Davidson
Evaluation Of Select Publicly Available In Silico Methods For Predicting Functional Effects Of Missense Mutations In The Galns Gene, Kathryn Davidson
Dissertations, Masters Theses, Capstones, and Culminating Projects
The ability to sequence patient DNA has led to an explosion in the reports of mutations for a number of diseases. Frequently, published reports include in silico predictions of the probability that the mutations are disease-associated. The question asked here is how well these in silico methods predict the effects of new mutations, i.e., mutations not included in the dataset(s) used for training and testing the in silico method. To address this question, we examined mutations associated, or potentially associated, with Morquio A (MPS IVA), a rare, autosomal recessive lysosomal storage disorder (LSD) caused by a deficiency of lysosomal enzyme …