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Effects Of Selenium On The Freshwater Alga Chlamydomonas Reinhardtii With And Without The Proteasome Inhibitor Mg132, E. Patrick Vallentine
Effects Of Selenium On The Freshwater Alga Chlamydomonas Reinhardtii With And Without The Proteasome Inhibitor Mg132, E. Patrick Vallentine
Honors Theses
Selenium is a required nutrient in animal life but has not been shown to be needed in plants. The model plant cell Chlamydomonas reinhardtii may be an exception as it appears to increase its growth rate in the presence of selenium. Because selenium has the same valence state as sulfur it may be incorporated into proteins as the modified amino acid selenocysteine. This incorporation of selenocysteine into protein may disrupt the disulfide bridges that give proteins the correct conformation. Misfolded proteins can wreak havoc on a cell. This stress can be measured through several parameters. Chlorophyll levels, glutathione levels, growth, …
The Involvement Of 26s Proteasome Complex In Selenium Toxicity, Melissa Sabbagh
The Involvement Of 26s Proteasome Complex In Selenium Toxicity, Melissa Sabbagh
Honors Theses
Plants that hyperaccumulate elements like selenium to potentially toxic levels may use proteasome complexes to reduce toxicity. The 26S proteasome complex may be a pathway that these plants take to rid themselves of selenium toxicity by destroying damaged proteins caused by selenium. A method to test this hypothesis is to use a hyperaccumulator of selenium Stanleya pinnata and nonhyperaccumulators Populous tremula and Arabdopsis thaliana to evaluate their proteasome abundance without and with selenium. To compare these species western blots were made to show the differences in proteasome abundance. Also to compare the amount of oxidized and an ubiquinated protein in …