Leveraging The Covid-19 Fermentation Trend To Enhance Nutrition And Food Safety Extension Efforts, Thais M. Ramos, Hanna Louvau, Heesun Kim, Maria L. Marco, Erin Dicaprio

The Journal of Extension

Our program aimed to increase knowledge related to fermented foods. Over 400 stakeholders registered for a webinar series that focused on defining fermented foods, health benefits of fermenting foods, and the safety of fermented foods. Participants indicated increases in knowledge and overall satisfaction with the content of the fermentation curriculum. The impact of the COVID-19 pandemic on the program outcomes are discussed.

Understanding Current Labor Shortage And Mechanization In New Jersey Nursery Crop Operations, Hemant Gohil, Timothy Waller, Raul Cabrera

The Journal of Extension

We conducted a survey of New Jersey’s nursery industry to understand the current levels of labor shortage, how this has affected specific tasks related to the production capacity of the industry, and the actual level of mechanization. Survey results identified priority areas needing mechanization. There is a need for greater mechanization in almost all areas of nursery production to reduce the reliance on labor and or improve the efficiency of labor. Other Implications related to Extension programming on nursery mechanization are also discussed.

Nutrition Education In Food Pantries: Perceptions Of Pantry Personnel Towards Implementation, Chelsea Allison, Christopher T Sneed, Marissa Mcelrone, Kristin Riggsbee, Janie Burney

The Journal of Extension

Extension programming can be effective at delivering nutrition education to food pantry clients. This study aimed to understand the perceptions of food pantry personnel towards nutrition education. A mixed methods survey was administered to food pantry personnel (n=53). Most (62.3%) reported their food pantry was church-affiliated, and few (22.6%) reported any Extension collaboration. Qualitative themes included perceptions that nutrition education was resource intensive, clients were not interested in nutrition education, and differing attitudes towards nutrition education. When working with food pantries, Extension should educate personnel about the importance of nutrition education, offer resources, and facilitate programming.

Connecting Diverse Communities Through A 4-H Urban-Rural Experience, Laurie Murrah-Hanson, Brittani Lee, Pamela Bloch

The Journal of Extension

Misconceptions regarding agricultural careers are exacerbated by increasing urbanization and are one of many differences in perspective between urban and rural communities. The One Georgia 4-H Urban-Rural Experience addresses this divide through a statewide youth exchange focusing on the agricultural sector. The program includes a multifaceted look at agricultural careers through visits to urban and rural communities. Focus group evaluations revealed the impact on youth through exposure to new careers, increased understanding of the diversity of agriculture, awareness of the interconnected nature of urban and rural communities, and a sense of belonging to a diverse group of youth.

New Mexico 4-H Online Horse Bowl Tournament, Jason L. Turner, Ryan W. Craig, Talisha L. Valdez, Halee M. Prather, Zane E. Webster, Sara K. Marta, Mindy G. Turner

The Journal of Extension

Under COVID-19 public health order constraints, Extension professionals used readily available technology (e.g., Google Forms, Zoom, Facebook Live, and QuizBowl Pro) to conduct a traditional 4-H quiz bowl contest entirely in a virtual setting as an innovative means of offering positive youth development in a structured environment when most New Mexicans were homebound and unable to travel. Participation in the virtual contest was substantially greater than similar in-person contests in the preceding five-year period, and youth reported an increase in equine knowledge as well as life skill development.

Challenges Experienced By Aquaponic Hobbyists, Producers, And Educators, D Allen Pattillo, David J. Cline, Janelle V. Hager, Luke A. Roy, Terrill R. Hanson

The Journal of Extension

We used an online survey to document challenges experienced by aquaponic hobbyists (n = 81), producers (n = 117), and educators (n = 75). Responses were distilled into the following categories: 1) operations and management; 2) facilities, location, and system design; 3) knowledge and educational resources; 4) funding; 5) economic viability; 6) plant culture; 7) marketing and distribution; 8) fish culture; 9) human factors; 10) regulations and certifications. Training and research in these areas are needed to advance the aquaponics industry.

Estimated Impact Of X-Disease And Little Cherry Disease In Washington And Oregon From 2015 To 2020, Cody Molnar, Tianna Dupont, Ashley Thompson

The Journal of Extension

X-disease phytoplasma and little cherry virus 1 and 2 have resulted in substantial loss of stone fruit acreage in Washington and Oregon. The extent and financial impact on the industry was not previously known. A Washington and Oregon State University survey documented 238,856 trees equivalent to 974 acres of sweet cherries removed due to X-disease and little cherry disease between 2015 and 2020. Removed trees reduced revenue to the industry by an estimated $30 million in 2020 and $65 million between 2015 and 2020. Over the seven-year re-establishment period estimated lost revenue and establishment costs to growers is an estimated …

Is There An Economic Advantage To Planting Diverse Summer Annual Forage Mixtures?, Kelly Mercier, Chris Teutsch, Ray Smith, Kenny Burdine, Edwin Ritchey, Eric Vanzant

The Journal of Extension

This study examined economic implications of planting summer annual mixtures of grasses, legumes, and forbs at varying nitrogen rates. No differences in yield occurred between the three mixtures, indicating that mixtures with lowest seed cost will be most economical. Applying N resulted in yield increases of 12.26 lb DM per lb N applied. Although yield responses to N were positive, sensitivity analyses showed that applying N resulted in positive net returns only when hay prices were high and N prices were low. When utilization rates are accounted for, enterprise budgets determined grazing to be 18% cheaper to implement than haying.

Development And Assessment Of A Food Safety Training Program For Farmers’ Market Vendors, Joshua Scheinberg, Rama Radhakrishna, Catherine Cutter

The Journal of Extension

Based on results collected through a comprehensive needs assessment of farmers market (FM) vendors in Pennsylvania, the purpose of this study was to develop and pilot-test a customized, food safety training program for FM vendors. A customized 3-hour, in-person, training program was developed and pilot tested. Using pre- and post-test assessment tests through piloted training, the results found participants scores on knowledge questions increased significantly by ~20% (p

Abundance And Territory Location Data Of Forest Songbirds To Determine Effects Of Pipeline Collocation As A Mitigation Strategy, Lillie Langlois

All Data Sets

These data were collected for the purpose of determining potential ecological costs associated with a mitigation strategy used in shale gas development in the Appalachian basin. Collocation is the practice of siting new shale gas pipelines adjacent to existing surface disturbance such as forest roads to reduce habitat fragmentation and loss of core forest. These data result from spot mapping for species of songbird at paired forest road and shale gas corridors (expanded access roads collocated with pipelines) between May and July 2013 in north-central Pennsylvania, USA. The data file contains nine sheets that report data from different pieces of …

The Role Of Fatty Acid Metabolism In The Pathogenesis Of Trypanosoma Brucei, Nava Poudyal

All Dissertations

Trypanosoma brucei is the protozoan parasite that causes African Sleeping Sickness in humans and nagana, a wasting disease in cattle. T. brucei completes its life cycle in two hosts, mammals and the tsetse fly insect vector. Due to the geographical restriction of the tsetse fly, the disease is endemic in sub-Saharan Africa. Both the insect and mammalian forms of the parasite need fatty acids to anchor their surface proteins. We worked on three projects on fatty acid metabolism and its role in immune evasion strategies of T. brucei. First, we assessed the role of T. brucei surface proteins in …

Exploring Soil-Tree Interactions After Applying Organic Matter Amendments To Peach Orchards In The Subtropical Climate Of The Southeastern United States, Brian Lawrence

All Dissertations

Soil disturbance from the cultivation and intensive management of orchards in hot and humid subtropical climates can reduce soil organic matter (OM). This research explored how the addition of OM amendments impact soil and tree parameters in two field studies and how proximity to OM may elicit unique genomic response of peach roots in a split-root pot study. The first field study applied two rates of compost (1x and 2x) and subsequently reduced fertilizer applications annually to a virgin and replant orchard location. While both compost rates successfully replaced fertilizers, only the 2x rate in the replant location increased soil …

Data-Driven Biomarker Panel Discovery In Ovarian Cancer Using Heterogenous Data Fusion On Exosomal And Non-Exosomal Microrna Expression Data, Paritra Mandal

All Dissertations

Ovarian cancer (OC) is an aggressive gynecological cancer and is currently the 5th leading cause of deaths due to cancer in women. High mortality rates are attributable to the vague pathogenesis and asymptomatic nature of the early stages. The development of a liquid biopsy for routine OC screening could help identify the disease at an earlier stage, making treatments more likely to be effective thereby increasing survival rates. Exosomes, small (~100nm) extracellular vesicles present in body fluids, have been shown to contain cancer-progression, onset, and related factors, making them good candidates for use in liquid biopsies. However, to date, only …

The Role Of Vsmc Mir-33a Expression On Apoa-I Mediated Cholesterol Efflux And Macrophage-Like Cell Transdifferentiation, Ikechukwu Esobi

All Dissertations

Atherosclerosis is a condition caused by cholesterol accumulating in arterial intimal cells and is a disease that kills more people in the United States and globally than any other disease. Atherosclerosis is commonly recognized to arise from arterial intimal macrophage cholesterol accumulation, but cell lineage tracing technology has shown that a large majority of cholesterol-laden intimal cells found in atherosclerotic arteries are actually vascular smooth muscle cells that have switched phenotypes to a macrophage-like cell. This vascular smooth muscle cell to macrophage-like cell phenotypic switch is known as transdifferentiation and can be triggered by vascular smooth muscle cell cholesterol accumulation. …

The Characterization Of Microglia In The Murine Dorsal Telencephalon In Development And Disease, Victoria Neckles

All Dissertations

Microglia are the primary immune cells of the central nervous system and contribute substantially to both development and maintenance of the brain. Negative alterations to microglia often result in detrimental outcomes and significant effort is being made to understand these powerful cells. The goal of this research is to characterize microglia in the developing and diseased murine dorsal telencephalon. In vivo cell labeling and confocal microscopy identified a transient cell type, amoeboid microglia cells (AMCs), that appear to contribute to the development of microglia. Moreover, the spatial distribution and morphological change of microglia was thoroughly characterized during postnatal development. The …

An Evaluation Of Bovine Reproductive Function From The Microrna Expression In The Female To Performance Testing In The Male, Erin Amiss

All Theses

Reproductive function is crucial to the success of a beef or dairy herd. Both the female and male play significant roles in the outcome of bovine reproduction. The bull contributes to reproductive success in many ways, such as genetics. The initial role that the bull has in reproductive success is fertilization, whether natural or by artificial insemination. One method of assessing bull performance are central bull test stations, which are traditionally administered by a university, extension center, or are privately owned. Central bull tests assess the performance of bulls from various management systems, breeds, and ages. Some measurements taken during …

Behavioral Ecology Of The American Alligator (Alligator Mississippiensis) In Human-Dominated Landscapes Of Coastal South Carolina, Anjelika Kidd-Weaver

All Dissertations

When wildlife habitat is developed to accommodate growing human populations, wildlife are forced to move to undisturbed landscapes or to acclimate to a novel, anthropogenic environment. In this dissertation, we investigated the behavior and ecology of the American alligator (Alligator mississippiensis) in coastal, residential resort communities with an overarching goal of identifying behavioral patterns of alligators that can inform risk management strategies. In Chapter One, we compared the space use of male and female alligators in primarily wetland versus residential landscapes across three seasons to determine if and how space use behaviors of alligators differ. We found that …

Characterization Of Colletotrichum Nymphaeae Isolates From Apple With Reduced Sensitivity To Fluazinam And Tebuconazole, Rulyu Meng

All Theses

Apple bitter rot is caused by Colletotrichum nymphaeae and other Colletotrichum species and management relies primarily on synthetic pesticides. Very few fungicides are effective against the disease and resistance has further limited their usefulness. A recent study indicated relatively low EC₅₀ values (the concentration required to inhibit 50% of mycelial growth in vitro) of C. nymphaeae isolates from Brazilian apples to fluazinam and tebuconazole, two fungicides that are not routinely used for bitter rot control. Isolates on opposite sides of the sensitivity range were designated either sensitive (lowest EC₅₀ values) and reduced-sensitive (highest EC₅₀ values). The objective …

Evaluation Of Novel Management Options For Bacterial Spot Of Peach, Daniela Moreno

All Theses

Xanthomonas arboricola pv. pruni (Xap) is an economically important pathogen causing bacterial spot of stone fruits worldwide. In the southeastern US management for this disease relies on copper and oxytetracycline (OTC) sprays, yet disease incidence remains high especially when the environmental conditions are conducive (warm and rainy). To further complicate disease management copper tolerant and OTC resistant (OTC^R) strains of Xap have been reported in the southeastern peach orchards. complicating disease management. Therefore, we aim to evaluate potential chemical and biological alternatives for disease management. The first objective was to evaluate a novel nano nanoformulation Bz (Zinc Borate) …

Addressing Meloidogyne Enterolobii Spread In The Sweetpotato Industry: Development Of A High Throughput Survey Detection Method For Root-Knot Nematodes And Quantification Of M. Enterolobii Damage On Storage Roots In Long-Term Assays, Julianna Culbreath

All Theses

Meloidogyne enterolobii is an aggressive root-knot nematode (RKN) species that has emerged as a significant pathogen of sweetpotato in the Southeastern US. Meloidogyne enterolobii is spread through the movement of infected ‘seed’ sweetpotatoes used for propagation, and the RKN resistance in commercial sweetpotato cultivars has proven ineffective against this nematode. This has prompted regulatory agencies to impose quarantines on regions infected with M. enterolobii. Sweetpotato storage roots can be rendered unmarketable due to M. enterolobii infection, resulting in potential overall yield loss. Visual detection of RKN in sweetpotato can be unreliable, and further distinguishing M. enterolobii from other RKN species …

Development, Characterization, And Application Of Pearl Millet Starch-Based Nanocomposite Films Reinforced With Kudzu Cellulose Nanocrystals, Sneh Bangar

All Dissertations

The accumulation of plastic waste has created serious human, wildlife, and environmental problems. Because of increasing consumer consumption, post-consumer packaging has become a major source of plastic waste. Therefore, there is a great need to develop alternative packaging materials which are environment-friendly, economical, and effective. The current research focused on developing pearl millet starch (PMS)-based biopolymer films reinforced with kudzu cellulose nanocrystals (CNCs) as an alternative to plastic packaging. Nanocomposite films were formulated by blending PMS and glycerol (30%) with different kudzu CNCs concentrations (1–7wt%) using the solution casting process. The prepared PMS/kudzu CNCs nanocomposite films were tested for various …

Combining Network Modeling And Experimental Approaches To Predict Drug Combination Responses, Deepraj Sarmah

All Dissertations

Cancer is a lethal disease and complex at multiple levels of cell biology. Despite many advances in treatments, many patients do not respond to therapy. This is owing to the complexity of cancer-genetic variability due to mutations, the multi-variate biochemical networks within which drug targets reside and existence and plasticity of multiple cell states. It is generally understood that a combination of drugs is a way to address the multi-faceted drivers of cancer and drug resistance. However, the sheer number of testable combinations and challenges in matching patients to appropriate combination treatments are major issues.

Here, we first present a …

Machine Learning Models For Human Synapse Genomics, Anqi Wei

All Dissertations

In the central nervous system, synapses are essential junctions that connect neurons and play important roles in neurotransmission and synaptic plasticity. While there are many challenges in human synapse genomics, machine learning techniques, which are capable of mining and interpreting large amounts of genomic data, may be utilized to facilitate the functional studies of human synapses. In this study, we have developed machine learning models for human synapse genomics to address several biological problems.

RNA localization plays an important role at the synapse, allowing local protein synthesis required for synaptic plasticity during brain development. Previous studies were conducted in mice …

The Effect Of Freezing Rates And Storage On Atlantic Salmon And Peaches, Wesam Aljeddawi

All Dissertations

The effect of long-term frozen storage of Atlantic salmon and sliced peaches was studied to determine methods to optimize quality. The first experiment was conducted to determine the quality changes in Atlantic salmon stored at different freezer temperatures over 12 months. Fresh and pre-frozen salmon were placed in five different freezers set at –7°C, –12°C, –18°C, –29°C and –77°C and evaluated for quality at 30, 90, 180, 270 and 360 days of storage. In general, higher quality was retained to a greater extent in salmon held at –29°C and –77°C for 360 days compared to the other storage temperatures. No …

Etiology And Epidemiology Of Mini-Ring In Ultradwarf Bermudagrass Putting Greens, Lukas Dant

All Dissertations

Mini-ring is a disease in ultradwarf bermudagrass (UDBG) [Cynodon dactylon (L.) Pers. × C. transvaalensis (Burtt-Davy)] putting greens caused by Waitea zeae (Voorhees) J.A. Crouch & Cubeta, (formerly Rhizoctonia zeae). Symptoms typically resemble frog-eye patches that are 10 to 40 cm in diameter with a bronze to orange outer ring and green center. In the southeastern United States, mini-ring symptoms appear in late-summer and generally persist until UDBG dormancy in late-fall. Mini-ring is often problematic in UDBG when nitrogen (N) fertility is reduced to manage organic matter production and improve putting green performance and perceived green speed. While …

Green On The Map - The Influence Of Conservation Easements On The Naturalness Of Landscapes In The United States, Nakisha Fouch

All Dissertations

Large protected areas have long been the cornerstone of conservation biology, however, in an era branded by the human dominance of ecosystems, regional landscape structure and function are often a consequence of accumulated land-use decisions that may or may not include a nod to conservation planning. With underrepresentation of habitats in publicly protected areas, attention has focused on the function of alternative land conservation mechanisms. Private conservation easements (CEs) have proliferated in the United States, yet assessing landscape-level function is confounded by holder and donor intent, national and regional policy, regional landscape contexts, varying extents, resolution, and temporal scale. Over …

Developmental Effects Of Chronic Low-Level Arsenic Exposure In Mouse Embryonic Stem Cells And In Human Induced Pluripotent Stem Cells, M. Chiara Perego

All Dissertations

Arsenic is an environmental contaminant commonly found in food and drinking water. Exposure to arsenic during embryonic development has been linked to reduced muscle growth, disrupted muscle development and locomotor activity, impaired neurodevelopment, reduced IQ, impaired memory and learning deficits. While the mechanisms responsible for developmental changes following in utero exposure to arsenic are not well known, one possibility is that arsenic might disrupt proper cellular differentiation. Therefore, we aimed to investigate the mechanisms by which arsenic exposure could alter stem cell differentiation into neurons.

First, we continuously exposed P19 mouse embryonic stem (ES) cells to 0.1 μM (7.5 ppb) …

X-Ray Interrogated Implantable Chemical And Strain Sensors For Monitoring Implant Associated Infections And Fracture Healing, Apeksha Rajamanthrilage

All Dissertations

Bone fracture healing includes complex and sequence of dynamic events to restore the integrity and biomechanical properties of the bone. While most of the fractures heal without any problem, healing is sometimes compromised. Two significant fracture healing complications are orthopedic implant-associated infections and non-unions/delayed union. These can be interrelated causes as well. Implant-associated infection can cause implant loosening, and as a result, it can delay the fracture healing process. Herein, we describe two different types of sensors that can be used in monitoring biochemical and biomechanical processes of fracture healing using X-rays.

We developed a XELCI (X-ray Excited Luminescence Chemical …

Effect Of Chlorine Dioxide Gas On The Properties Of Packaging Materials And Effect Of Chlorine Dioxide Gas To Maintain The Quality Of Fresh Strawberries, Duleeka Kuruwita, Duleeka Prasadani Kuruwita Arachchige

All Dissertations

ClO₂ as an antimicrobial gas in the headspace of produce package is a relatively novel approach. Gaseous ClO₂ is more effective than aqueous ClO₂ and can be used in the headspace of fresh food packages. ClO₂ gas can diffuse into product surfaces and films. As an oxidizer, it can react with and change polymeric package components, possibly affecting the product’s shelflife.

This research studied effects of ClO₂ gas treatments on produce packaging materials (APET, two PE types, Nylon). The treatment group (with ClO₂) and the control group (without ClO₂) of packaging …

Large Genomes Assembly Using Mapreduce Framework, Yuehua Zhang

All Dissertations

Knowing the genome sequence of an organism is the essential step toward understanding its genomic and genetic characteristics. Currently, whole genome shotgun (WGS) sequencing is the most widely used genome sequencing technique to determine the entire DNA sequence of an organism. Recent advances in next-generation sequencing (NGS) techniques have enabled biologists to generate large DNA sequences in a high-throughput and low-cost way. However, the assembly of NGS reads faces significant challenges due to short reads and an enormously high volume of data. Despite recent progress in genome assembly, current NGS assemblers cannot generate high-quality results or efficiently handle large genomes …