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Folian-Cv1 Is A Member Of A Highly Acidic Phosphoprotein Class Derived From The Foliated Layer Of The Eastern Oyster (Crassostrea Virginica) Shell And Identified In Hemocytes And Mantle, Mary Beth Johnston, A. P. Wheeler, Elizabeth P. Falwell, Meg E. Staton, Christopher A. Saski, Andrew S. Mount
Folian-Cv1 Is A Member Of A Highly Acidic Phosphoprotein Class Derived From The Foliated Layer Of The Eastern Oyster (Crassostrea Virginica) Shell And Identified In Hemocytes And Mantle, Mary Beth Johnston, A. P. Wheeler, Elizabeth P. Falwell, Meg E. Staton, Christopher A. Saski, Andrew S. Mount
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The proteins derived from the foliated shell layer of the oyster, Crassostrea virginica, are unusually acidic and highly phosphorylated. Here we report the identification of a gene encoding a member of this class of phosphoproteins that we collectively refer to as folian. Using an in silico approach, a virtual probe was constructed from an N-terminal sequence (DEADAGD) determined for a 48 kDa folian phosphoprotein and used to screen an oyster EST databank. A sequence that matched the N-terminus of the 48 kDa protein was found and used to identify the full-length gene from a C. virginica BAC library. The …