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Life Sciences

Clemson University

2012

Turfgrass

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Foliar Applied Urea Nitrogen Metabolism In Warm-Season Turfgrass Under Salinity Stress, Nicholas Menchyk Dec 2012

Foliar Applied Urea Nitrogen Metabolism In Warm-Season Turfgrass Under Salinity Stress, Nicholas Menchyk

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The most widely used foliar nitrogen (N) source for warm-season turfgrass and agriculture is urea [(NH2)2CO], due to its low cost, high percentage of N (46% by mass), and completely soluble nature. Since urea is a soluble N source, it is commonly utilized as a foliar N source when tank mixed with pesticides in warm-season turfgrass management. The N in urea is not directly available to the plant until it is hydrolyzed into ammonia by the enzyme urease in the cytosol. Urease is a nickel (Ni2+) dependant enzyme that is ubiquitous in plants. Its main biochemical function is the hydrolysis …


Genetic Engineering Of Turfgrass For Enhanced Performance Under Environmental Stress, Man Zhou May 2012

Genetic Engineering Of Turfgrass For Enhanced Performance Under Environmental Stress, Man Zhou

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Turfgrass species are agriculturally and economically important perennial crops that are susceptible to biotic stress (e.g. fungal pathogens) and abiotic stress (e.g. salinity and drought). Every year, environmental stress significantly influences turfgrass quality and production causing economic loss globally. My research explores the feasibility of using two novel transgenes - Penaeidin4-1 (Pen4-1) from the shrimp, Litopenaeus setiferus, and microRNA319a (miR319a) from the rice, Oryza sativa, to genetically engineer turfgrass for enhanced tolerance to environmental stress.
The antimicrobial peptide - Pen4-1 has been reported to possess in vitro antifungal and antibacterial activities against various economically important pathogens. In this study, two …