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A Role For A Rab4-Like Gtpase In Endocytosis And In Regulation Of Contractile Vacuole Structure And Function In Dictyostelium Discoideum, John Bush, Lesly A. Temesvari, Juan Rodriguez-Paris, Greg Buczynski, James Cardelli
A Role For A Rab4-Like Gtpase In Endocytosis And In Regulation Of Contractile Vacuole Structure And Function In Dictyostelium Discoideum, John Bush, Lesly A. Temesvari, Juan Rodriguez-Paris, Greg Buczynski, James Cardelli
Publications
The small Mr Rab4-like GTPase, RabD, localizes to the endosomal pathway and the contractile vacuole membrane system in Dictyostelium discoideum. Stably transformed cell lines overexpressing a dominant negative functioning RabD internalized fluid phase marker at 50% of the rate of wild-type cells. Mutant cells were also slower at recycling internalized fluid. Microscopic and biochemical approaches indicated that the transport of fluid to large postlysosome vacuoles was delayed in mutant cells, resulting in an accumulation in acidic smaller vesicles, probably lysosomes. Also, RabD N121I-expressing cell lines missorted a small but significant percentage of newly synthesized lysosomal alpha-mannosidase precursor polypeptides. However, the …
Involvement Of The Vacuolar Proton-Translocating Atpase In Multiple Steps Of The Endo-Lysosomal System And In The Contractile Vacuole System Of Dictyostelium Discoideum, Lesly A. Temesvari, Juan M. Rodriguez-Paris, John M. Bush, Linyi Zhang, James A. Cardelli
Involvement Of The Vacuolar Proton-Translocating Atpase In Multiple Steps Of The Endo-Lysosomal System And In The Contractile Vacuole System Of Dictyostelium Discoideum, Lesly A. Temesvari, Juan M. Rodriguez-Paris, John M. Bush, Linyi Zhang, James A. Cardelli
Publications
We have investigated the effects of Concanamycin A (CMA), a specific inhibitor of vacuolar type H(+)-ATPases, on acidification and function of the endo-lysosomal and contractile vacuole (CV) systems of D. discoideum. This drug inhibited acidification and increased the pH of endo-lysosomal vesicles both in vivo and in vitro in a dose dependent manner. Treatment also inhibited endocytosis and exocytosis of fluid phase, and phagocytosis of latex beads. This report also confirms our previous conclusions (Cardelli et al. (1989) J. Biol. Chem. 264, 3454–3463) that maintenance of acidic pH in lumenal compartments is required for efficient processing and targeting of a …
Examination Of The Endosomal And Lysosomal Pathways In Dictyostelium Discoideum Myosin I Mutants, Lesly A. Temesvari, John M. Bush, Michelle D. Peterson, Kristine D. Novak, Margaret A. Titus, James A. Cardelli
Examination Of The Endosomal And Lysosomal Pathways In Dictyostelium Discoideum Myosin I Mutants, Lesly A. Temesvari, John M. Bush, Michelle D. Peterson, Kristine D. Novak, Margaret A. Titus, James A. Cardelli
Publications
The role of myosin Is in endosomal trafficking and the lysosomal system was investigated in a Dictyostelium discoideum myosin I double mutant myoB-/C-, that has been previously shown to exhibit defects in fluid-phase endocytosis during growth in suspension culture (Novak et al., 1995). Various properties of the endosomal pathway in the myoB-/C- double mutant as well as in the myoB- and myoC- single mutants, including intravesicular pH, and intracellular retention time and exocytosis of a fluid phase marker, were found to be indistinguishable from wild-type parental cells. The intimate connection between the contractile vacuole complex and the endocytic pathway in …
Book Review - The Adult Limnephilus Leach (Trichoptera:Limnephilidae) Of The New World, John C. Morse
Book Review - The Adult Limnephilus Leach (Trichoptera:Limnephilidae) Of The New World, John C. Morse
Publications
No abstract provided.
Book Review - Caddisflies (Trichoptera) Of The Interior Highlands Of North America, John C. Morse
Book Review - Caddisflies (Trichoptera) Of The Interior Highlands Of North America, John C. Morse
Publications
No abstract provided.