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Characterization Of Lysosomal Membrane Proteins Of Dictyostelium Discoideum, Lesly A. Temesvari, Juan Rodriguez-Paris, John Bush, Theodore L. Steck, James Caradelli
Characterization Of Lysosomal Membrane Proteins Of Dictyostelium Discoideum, Lesly A. Temesvari, Juan Rodriguez-Paris, John Bush, Theodore L. Steck, James Caradelli
Publications
Highly purified lysosomes, prepared by magnetic fractionation of homogenates from Dictyostelium discoideum cells fed colloidal iron, were lysed under hypoosmotic conditions, and the membrane-associated proteins were subjected to gel electrophoresis. Thirteen major membrane polypeptides, ranging in molecular weight from 25,000 to 100,000 were identified. The isoelectric points of these proteins ranged from below 3.8 to greater than 7.0. Most of these proteins were stripped from membranes exposed to a chaotropic agent, 3,5-diodo-2-hydroxybenzoic acid lithium salt, and were therefore classified as peripheral membrane proteins. Twenty five glycoprotein species were detected by lectin blot analysis; 19 were classified as integral membrane proteins, …
A Rab4-Like Gtpase In Dictyostelium Discoideum Colocalizes With V-H(+)-Atpases In Reticular Membranes Of The Contractile Vacuole Complex And In Lysosomes, John Bush, Kathleen Nolta, Juan Rodriguez-Paris, Nancy Kaufmann, Theresa O'Halloran, Tracy Ruscetti, Lesly A. Temesvari, Theodore Steck, James Cardelli
A Rab4-Like Gtpase In Dictyostelium Discoideum Colocalizes With V-H(+)-Atpases In Reticular Membranes Of The Contractile Vacuole Complex And In Lysosomes, John Bush, Kathleen Nolta, Juan Rodriguez-Paris, Nancy Kaufmann, Theresa O'Halloran, Tracy Ruscetti, Lesly A. Temesvari, Theodore Steck, James Cardelli
Publications
In the course of screening a cDNA library for ras-related Dictyostelium discoideum genes, we cloned a 0.7 kb cDNA (rabD) encoding a putative protein that was 70% identical at the amino acid level to human Rab4. Rab4 is a small M(r) GTPase, which belongs to the Ras superfamily and functions to regulate endocytosis in mammalian cells. Southern blot analysis indicated that the rabD cDNA was encoded by a single copy gene while Northern blot analysis revealed that the rabD gene was expressed at relatively constant levels during growth and differentiation. Affinity-purified antibodies were prepared against a RabD fusion protein expressed …
Ceraclea Enodis, A New Species Of Sponge-Feeding Caddisfly (Trichoptera:Leptoceridae) Previously Misidentified, H Nicole Whitlock, John C. Morse
Ceraclea Enodis, A New Species Of Sponge-Feeding Caddisfly (Trichoptera:Leptoceridae) Previously Misidentified, H Nicole Whitlock, John C. Morse
Publications
A new species of Trichoptera, Ceraclea (C.) enodis, of the Ceraclea (C.) senilis Group, was discovered in freshwater sponge in the Little River in Montgomery County, North Carolina. Illustrations and descriptions of the male, female, larva, pupa, and larval case are given. Adults resemble those C. cancellata (Betten), the mature larva looks like that of C. maculata (Banks), the younger larva may be confused with C. transverasa (Hagen), and the pupa has anal rods rather like those of C. spngillovorax (Resh); but all life history forms can be distinguished reliably from these species by consistent morphological characters and the sponge …
Mass Handling Of Watermelon Microcuttings, Jeffrey Adelberg, Y Alper, R E. Young, B B. Rhodes
Mass Handling Of Watermelon Microcuttings, Jeffrey Adelberg, Y Alper, R E. Young, B B. Rhodes
Publications
Modifications were made in the configurations of the unitizing, nonselective wire cutters used by Alper et al.(1992) for mass cuttings of Stage II Citrullus lanatus cv. Charlee (watermelon) plant tissue cultures to further enhance productivity. Mounting the cutter in an inverted position over the receiving vessel eliminated time required for filling. This cut-and-dump technique became 4.8 times more productive for the total transfer process than the conventional scalpel and forceps technique when both time and yield of cut segments with visible buds were considered. A concept for growing fewer, larger tissue clusters per vessel in mini-trays with orienting cells and …
Unitized, Nonselective Cutting Of In Vitro Watermelon, Jeff W. Adelberg, Y Alper, R E. Young, B B. Rhodes
Unitized, Nonselective Cutting Of In Vitro Watermelon, Jeff W. Adelberg, Y Alper, R E. Young, B B. Rhodes
Publications
Unitized, nonselective mass cutting of Stage II Citrullus lanatus cv. Charlee (watermelon) plant tissue cultures has been achieved with various configurations and sizes of wire cutter devices. Time studies revealed that the wire cutters increased the productivity of the cutting function over conventional scalpel and forceps by a factor of 14, Total transfer productivity, including the manual functions of removing tissue from an initial culture vessel and filling (sorting and placing cut tissue segments) new vessels, was increased by a factor of 1,8, The square grid-type cutting devices yielded from 48 to 59% as many viable bud clusters per culture …