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Life Sciences

Clemson University

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Biofilms

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Investigating The Impact Of Novel Iron Oxide Nanoparticles On Legionella Pneumophila Biofilms And Trophic Interactions, Brennen Jenkins May 2014

Investigating The Impact Of Novel Iron Oxide Nanoparticles On Legionella Pneumophila Biofilms And Trophic Interactions, Brennen Jenkins

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Microbial biofilms serve as the base of food webs and are important for nutrient cycling in aquatic ecosystems. Nanoparticles (NPs) that enter into these aquatic systems have the potential to settle and become trapped within biofilms. As NPs become further integrated into consumer products, understanding their fate and effects on aquatic ecosystems is of paramount importance. Previous studies from our lab show that gold NPs induce dispersal of Legionella pneumophila biofilms. NPs with platinum and iron oxide core chemistries also lead to similar dispersal events, however, silver core NPs do not seem to induce these events due to NP aggregation. …


Effects Of Larval Exposure To Bacterial Species In Three Genera On Life History Traits And Oviposition Behavior In The Southern House Mosquito, Culex Quinquefasciatus, Jonathan Gravgaard Dec 2011

Effects Of Larval Exposure To Bacterial Species In Three Genera On Life History Traits And Oviposition Behavior In The Southern House Mosquito, Culex Quinquefasciatus, Jonathan Gravgaard

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The biofilm is the most common niche for microorganisms living in aquatic environments. Forming a biofilm provides the means to resist shear forces, adverse osmolarity, chemical agents, and other environmental stressors. Biofilms also provide a major food source for aquatic arthropods like mosquito larvae. Culex quinquefasciatus larvae are found in freshwater pools with high organic content, and have the ability to graze on bacterial biofilms. To study how C. quinquefasciatus mosquitoes interact with bacteria in their environment I developed an experimental system that allows mosquito larvae to freely graze on bacteria in either the planktonic or biofilm state. The efficacy …


Gold Nanoparticle Interactions And Impact Upon A Common Biofilm Source: Legionella Pneumophila , Amber Stojak Dec 2009

Gold Nanoparticle Interactions And Impact Upon A Common Biofilm Source: Legionella Pneumophila , Amber Stojak

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There exists widespread concern of pathogenic bacteria colonizing and establishing biofilms in water systems providing a direct exposure route to the public. Legionella are widely distributed in human-made and natural environments, and colonization lends the potential to infect human hosts that may lead to the development of Legionnaire's Disease. Silver has been used in cooling towers and water filtration systems for removal of planktonic bacteria and biofilms; however permanent removal of L. pneumophila biofilms with metal ions is impossible when established biofilms are in hard to access places such as dead end piping or inside a protozoan host. Bulk metals …