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Is Population Genetics Mired In The Past?, Andrew J. Bohonak, Neil Davies, George K. Roderick, Francis X. Villablanca
Is Population Genetics Mired In The Past?, Andrew J. Bohonak, Neil Davies, George K. Roderick, Francis X. Villablanca
Biological Sciences
No abstract provided.
Terminal Restriction Fragment Patterns (Trfps), A Rapid, Pcr-Based Method For The Comparison Of Complex Bacterial Communities, Brian G. Clement, Lucia E. Kehl, Kristin L. Debord, Christopher L. Kitts
Terminal Restriction Fragment Patterns (Trfps), A Rapid, Pcr-Based Method For The Comparison Of Complex Bacterial Communities, Brian G. Clement, Lucia E. Kehl, Kristin L. Debord, Christopher L. Kitts
Biological Sciences
Microbial populations in complex environmental samples are difficult to characterize; current techniques are incomplete and time consuming. We investigated a polymerase chain reaction (PCR)-based method for rapidly comparing bacterial communities independent of culture or cloning. Community 16S rRNA genes were amplified and fluorescently labeled by PCR. The labeled products were digested by a restriction enzyme and the labeled, terminal restriction fragments (TRFs) were separated by electrophoresis and detected by laser-induced fluorescence on an automated gene sequencer. PCR parameters were optimized using an in vitro model community of known organisms. Community comparisons were made between deer fecal pellets, petroleum hydrocarbon-contaminated sands …