Digital Commons Network^™

Effects Of Doxorubicin On Extracellular Matrix Regulation In Primary Cardiac Fibroblasts From Mice, Cameron Skaggs, Steve Nick, Conner Patricelli, Laura Bond, Kali Woods, Luke Woodbury, Julia Thom Oxford, Xinzhu Pu

Biomolecular Research Center Publications and Presentations

Objective Doxorubicin (DOX) is a highly effective chemotherapeutic used to treat many adult and pediatric cancers. However, its use is limited due to a dose-dependent cardiotoxicity, which can lead to lethal cardiomyopathy. In contrast to the extensive research efforts on toxic effects of DOX in cardiomyocytes, its effects and mechanisms on cardiac extracellular matrix (ECM) homeostasis and remodeling are poorly understood. In this study, we examined the potential effects of DOX on cardiac ECM to further our mechanistic understanding of DOX-induced cardiotoxicity.

Results DOX-induced significant down-regulation of several ECM related genes in primary cardiac fibroblasts, including Adamts1, Adamts5, Col4a1, Col4a2, …

Social And Biological Perspectives To Investigate And Address Illegal Shooting Of Raptors, Eve Thomason, Kenneth Wallen, Todd Katzner

Raptor Research Center Publications and Presentations

Humans have shot raptors for centuries. However, in many countries these actions have been illegal since the mid-twentieth century. Despite this history, there is not a comprehensive understanding of the characteristics of this activity, its frequency, and why it occurs. We used literature review and principles drawn from ecology, sociology, and criminology to understand this problem. First, we review literature on raptor shooting globally to explore documented motivations for shooting and we describe the history of raptor shooting in the United States of America (USA). Then, to illustrate the contemporary frequency and geographic breadth of the shooting of raptors, we …

Importance Of Flood Irrigation For Foraging Colonial Waterbirds, Colleen E. Moulton, Jay D. Carlisle, Sonya J. Knetter, Kathryn Brenner, Robert A. Cavallaro

Intermountain Bird Observatory Publications and Presentations

For a colonial‐nesting bird, like the white‐faced ibis (Plegadis chihi), the landscape surrounding the breeding colony can be important. White‐faced ibis must rely on areas outside their breeding colony for foraging, but this part of their life history has received little attention, and the management of this landscape even less so. To address this knowledge gap, we conducted road‐based driving surveys and a randomly selected, spatially balanced sample survey of agricultural fields within a 22‐km radius of the 2 largest white‐ faced ibis breeding colonies in Idaho, USA: Market Lake Wildlife Management Area and Mud Lake Wildlife Management …

Short-Eared Owl Land-Use Associations During The Breeding Season In The Western United States, Robert A. Miller, Joseph B. Buchanan, Theresa L. Pope, Jay D. Carlisle, Colleen E. Moulton, Travis L. Booms

Intermountain Bird Observatory Publications and Presentations

The Short-eared Owl (Asio flammeus) is a species of conservation concern in the western USA, with evidence for declining population sizes. Monitoring of Short-eared Owls is complicated because of their low site fidelity and nomadic movements. We recruited community-science participants to implement a multi-year survey of Short-eared Owls across eight states in the western USA, resulting in a program of sufficient temporal and spatial dimensions to overcome many of the challenges in monitoring this species. We implemented both multi-scale occupancy and colonization/extinction modeling to provide insights into land-cover use, and to identify which cover types supported higher occurrence. …

Local Weather Explains Annual Variation In Northern Goshawk Reproduction In The Northern Great Basin, Usa, Allyson B. Bangerter, Eliana R. Heiser, Jay D. Carlisle, Robert A. Miller

Intermountain Bird Observatory Publications and Presentations

Weather is thought to influence raptor reproduction through effects on prey availability, condition of adults, and survival of nests and young; however, there are few long-term studies of the effects of weather on raptor reproduction. We investigated the effects of weather on Northern Goshawk (Accipiter gentilis; henceforth goshawk) breeding rate, productivity, and fledging date in south-central Idaho and northern Utah, USA. Using data from 42 territories where we found evidence of breeding attempts in ≥1 yr from 2011–2019, we analyzed breeding rates using 315 territory–season combinations, analyzed productivity for 134 breeding attempts, and analyzed fledging date for 118 …

Bioactive Recombinant Human Oncostatin M For Nmr-Based Screening In Drug Discovery, Olga A. Mass, Joseph Tuccinardi, Luke Woodbury, Cody L. Wolf, Bri Grantham, Kelsey Holdaway, Xinzhu Pu, Matthew D. King, Don L. Warner, Cheryl L. Jorcyk, Lisa R. Warner

Biomolecular Research Center Publications and Presentations

Oncostatin M (OSM) is a pleiotropic, interleukin-6 family inflammatory cytokine that plays an important role in inflammatory diseases, including inflammatory bowel disease, rheumatoid arthritis, and cancer progression and metastasis. Recently, elevated OSM levels have been found in the serum of COVID-19 patients in intensive care units. Multiple anti-OSM therapeutics have been investigated, but to date no OSM small molecule inhibitors are clinically available. To pursue a high-throughput screening and structure-based drug discovery strategy to design a small molecule inhibitor of OSM, milligram quantities of highly pure, bioactive OSM are required. Here, we developed a reliable protocol to produce highly pure …

Rapid Production And Purification Of Dye-Loaded Liposomes By Electrodialysis-Driven Depletion, Gamid Abatchev, Andrew Bogard, Zoe Hutchinson, Jason Ward, Daniel Fologea

Physics Faculty Publications and Presentations

Liposomes are spherical-shaped vesicles that enclose an aqueous milieu surrounded by bilayer or multilayer membranes formed by self-assembly of lipid molecules. They are intensively exploited as either model membranes for fundamental studies or as vehicles for delivery of active substances in vivo and in vitro. Irrespective of the method adopted for production of loaded liposomes, obtaining the final purified product is often achieved by employing multiple, time consuming steps. To alleviate this problem, we propose a simplified approach for concomitant production and purification of loaded liposomes by exploiting the Electrodialysis-Driven Depletion of charged molecules from solutions. Our investigations show that …

Decreased Cortisol Among Hikers Who Preferentially Visit And Value Biodiverse Riparian Zones, Ellie Opdahl, Kathryn Demps, Julie A. Heath

Anthropology Faculty Publications and Presentations

While outdoor recreationists often report increases to their well-being for time spent in nature, the mechanisms through which local ecologies affect human health have been difficult to quantify, and thus to manage. We combine data from pre-post salivary cortisol measures, GPS tracks, visitor photos, and surveys from 88 hikers traversing several types of landscape within peri-urban public lands in southwest Idaho, USA. We find that time in biodiverse riparian areas and areas of perceived aesthetic value correlates with decreases in salivary cortisol and improved well-being for hikers. Wildlife sightings were not associated with changes in salivary cortisol, but were associated …

Illegal Killing Of Nongame Wildlife And Recreational Shooting In Conservation Areas, Jay D. Carlisle, Eve C. Thomason, James R. Belthoff, Julie A. Heath, Kristina J. Parker, Heather M. Hayes, Madeline C. Alberg, Sandra M. Amdor, Steven E. Alsup, Stephanie E. Coates

Intermountain Bird Observatory Publications and Presentations

Illegal killing of nongame wildlife is a global yet poorly documented problem. The prevalence and ecological consequences of illegal killing are often underestimated or completely unknown. We review the practice of legal recreational shooting and present data gathered from telemetry, surveys, and observations on its association with illegal killing of wildlife (birds and snakes) within conservation areas in Idaho, USA. In total, 33% of telemetered long‐billed curlews (Numenius americanus) and 59% of other bird carcasses found with known cause of death (or 32% of total) were illegally shot. Analysis of spatial distributions of illegal and legal shooting is …

Investigation Of Multiphasic 3d-Bioplotted Scaffolds For Site-Specific Chondrogenic And Osteogenic Differentiation Of Human Adipose-Derived Stem Cells For Osteochondral Tissue Engineering Applications, Julia T. Oxford

Biomolecular Research Center Publications and Presentations

Osteoarthritis is a degenerative joint disease that limits mobility of the affected joint due to the degradation of articular cartilage and subchondral bone. The limited regenerative capacity of cartilage presents significant challenges when attempting to repair or reverse the effects of cartilage degradation. Tissue engineered medical products are a promising alternative to treat osteochondral degeneration due to their potential to integrate into the patient's existing tissue. The goal of this study was to create a scaffold that would induce site-specific osteogenic and chondrogenic differentiation of human adipose-derived stem cells (hASC) to generate a full osteochondral implant. Scaffolds were fabricated using …

Temporary Membrane Permeabilization Via The Pore-Forming Toxin Lysenin, Nisha Shrestha, Christopher A. Thomas, Devon Richtsmeier, Andrew Bogard, Rebecca Hermann, Malyk Walker, Gamid Abatchev, Raquel J. Brown, Daniel Fologea

Physics Faculty Publications and Presentations

Pore-forming toxins are alluring tools for delivering biologically-active, impermeable cargoes to intracellular environments by introducing large conductance pathways into cell membranes. However, the lack of regulation often leads to the dissipation of electrical and chemical gradients, which might significantly affect the viability of cells under scrutiny. To mitigate these problems, we explored the use of lysenin channels to reversibly control the barrier function of natural and artificial lipid membrane systems by controlling the lysenin’s transport properties. We employed artificial membranes and electrophysiology measurements in order to identify the influence of labels and media on the lysenin channel’s conductance. Two cell …

Long-Billed Curlew Nest Site Selection And Success In The Intermountain West, Stephanie E. Coates, Benjamin W. Wright, Jay D. Carlisle

Intermountain Bird Observatory Publications and Presentations

Grassland birds have experienced steeper population declines between 1966 and 2015 than any other bird group on the North American continent, and migratory grassland birds may face threats in all stages of their annual cycle. The grassland‐associated long‐billed curlew (Numenius americanus) is experiencing population declines in regional and local portions of their North American breeding range. The nesting period is an important portion of the annual cycle when curlews may face demographic rate limitations from a suite of threats including predators and anthropogenic disturbance. We compared nest sites to random sites within breeding territories to examine nest site …

High Expression Of Osm And Il-6 Are Associated With Decreased Breast Cancer Survival: Synergistic Induction Of Il-6 Secretion By Osm And Il-1Β, Ken Tawara, Hannah Scott, Jacqueline Emathinger, Cody Wolf, Dollie Lajoie, Danielle Hedeen, Laura Bond, Cheryl Jorcyk

Biomolecular Research Center Publications and Presentations

Chronic inflammation has been recognized as a risk factor for the development and maintenance of malignant disease. Cytokines such as interleukin-6 (IL-6), oncostatin M (OSM), and interleukin-1 beta (IL-1β) promote the development of both acute and chronic inflammation while promoting in vitro metrics of breast cancer metastasis. However, anti-IL-6 and anti-IL-1β therapeutics have not yielded significant results against solid tumors in clinical trials. Here we show that these three cytokines are interrelated in expression. Using the Curtis TCGA™ dataset, we have determined that there is a correlation between expression levels of OSM, IL-6, and IL-1β and reduced breast cancer patient …

Using Motion-Activated Trail Cameras To Study Diet And Productivity Of Cliff-Nesting Golden Eagles, Jordan T. Harrison, Michael N. Kochert, Benjamin P. Pauli, Julie A. Heath

Raptor Research Center Publications and Presentations

Studies of cliff-nesting raptors can be challenging because direct observations of nest contents are difficult. Our goals were to develop a protocol for installing motion-activated trail cameras at Golden Eagle (Aquila chrysaetos) nests to record diet information and productivity, and to estimate prey detection probability using different diet study methods. In 2014 and 2015, we installed cameras at 12 Golden Eagle nests with 18–42-d-old nestlings. Following installation, we monitored adult behavior using direct observation and post-installation image review. At two nests, adult eagles did not return to nests or exhibited behaviors suggesting avoidance of the cameras, but returned …

Extracellular Matrix In Development And Disease, Julia Thom Oxford, Jonathon C. Reeck, Makenna J. Hardy

Biomolecular Research Center Publications and Presentations

The evolution of multicellular metazoan organisms was marked by the inclusion of an extracellular matrix (ECM), a multicomponent, proteinaceous network between cells that contributes to the spatial arrangement of cells and the resulting tissue organization. The development of an ECM that provides support in larger organisms may have represented an advantage in the face of selection pressure for the evolution of the ECM.

Sex Determination By Morphological Measurements Of Young Rockhopper Penguins (Eudyptes Chrysocome) During The Crèche Phase, Virginia Morandini, Miguel Ferrer, Lynelle Perry, Marc Bechard

Raptor Research Center Publications and Presentations

To provide an easy and reliable work tool to identify the sex of Rockhopper Penguin (Eudyptes chrysocome) chicks, we weighed and measured 95 nestlings in the crèche phase during 24-31 January 2017 on the Falkland Islands, Argentina. Sex was subsequently determined using DNA analyses of blood from the same individuals. Significant differences were found in bill length (exposed culmen), bill depth and width, flipper length, and diagonal tarsus, but stepwise discriminant analysis showed bill length to be the best predictor for sex determination. Our model correctly classified 82.7% of males and 90.2% of females (overall correct classification 86.2%). …

Repeated Observations Of Northern Goshawks Foraging As Terrestrial Predators, Robert A. Miller

Intermountain Bird Observatory Publications and Presentations

The Northern Goshawk (Accipiter gentilis; hereafter goshawk) is a generalist predator occupying boreal and temperate forests of the Holarctic (Squires and Reynolds 1997). The diet of goshawks has been studied in many areas and varies substantially among study sites, but some avian and mammalian genera are consistently represented (Squires and Reynolds 1997, Squires and Kennedy 2006). Within the northern Great Basin of North America, the breeding-season diet of goshawks is often dominated by Belding's ground squirrels (Urocitellus beldingi, also known as Spermophilus beldingi; Younk and Bechard 1994, Miller et al. 2014). The local reliance upon …

First Record Of Nestling Relocation By Adult Gyrfalcons (Falco Rusticolus) Following Nest Collapse, Bryce W. Robinson, Neil Paprocki, David L. Anderson, Marc J. Bechard

Raptor Research Center Publications and Presentations

Nest collapse has been documented in many bird species, with little discussion of adult behavior following collapse. We present evidence of a partial collapse of a Gyrfalcon (Falco rusticolus) nest during the nestling period and the subsequent adult response. A nest camera captured the nest collapse and showed one adult Gyrfalcon holding a live nestling in its beak before leaving the nest. Later, we found the surviving nestling alive in an alternate nest 5 m from the original nest, presumably transported there by the adult. We believe this is the first report of an adult Gyrfalcon moving a …

Serum Proteins Enhance Dispersion Stability And Influence The Cytotoxicity And Dosimetry Of Zno Nanoparticles In Suspension And Adherent Cancer Cell Models, Catherine B. Anders, Jordan J. Chess, Denise G. Wingett, Alex Punnoose

Biomolecular Research Center Publications and Presentations

Agglomeration and sedimentation of nanoparticles (NPs) within biological solutions is a major limitation in their use in many downstream applications. It has been proposed that serum proteins associate with the NP surface to form a protein corona that limits agglomeration and sedimentation. Here, we investigate the effect of fetal bovine serum (FBS) proteins on the dispersion stability, dosimetry, and NP-induced cytotoxicity of cationic zinc oxide nanoparticles (nZnO) synthesized via forced hydrolysis with a core size of 10 nm. Two different in vitro cell culture models, suspension and adherent, were evaluated by comparing a phosphate buffered saline (PBS) nZnO dispersion (nZnO/PBS) …

Wintering Bald Eagle Count Trends In The Conterminous United States, 1986-2010, Wade L. Eakle, Laura Bond, Mark R. Fuller, Richard A. Fischer, Karen Steenhof

Biomolecular Research Center Publications and Presentations

We analyzed counts from the annual Midwinter Bald Eagle Survey to examine state, regional, and national trends in counts of wintering Bald Eagles (Haliaeetus leucocephalus) within the conterminous 48 United States from 1986 to 2010. Using hierarchical mixed model methods, we report trends in counts from 11 729 surveys along 844 routes in 44 states. Nationwide Bald Eagle counts increased 0.6% per yr over the 25-yr period, compared to an estimate of 1.9% per yr from 1986 to 2000. Trend estimates for Bald Eagles were significant (P ≤ 0.05) and positive in the northeastern and northwestern U.S. …

The Effect Of Osm On Mc3t3-E1 Osteoblastic Cells In Simulated Microgravity With Radiation, Jake Goyden, Ken Tawara, Danielle Hedeen, Jeffrey S. Willey, Julia Thom Oxford, Cheryl L. Jorcyk

Biomolecular Research Center Publications and Presentations

Bone deterioration is a challenge in long-term spaceflight with significant connections to patients experiencing disuse bone loss. Prolonged unloading and radiation exposure, defining characteristics of space travel, have both been associated with changes in inflammatory signaling via IL-6 class cytokines in bone. While there is also evidence for perturbed IL-6 class signaling in spaceflight, there has been scant examination of the connections between microgravity, radiation, and inflammatory stimuli in bone. Our lab and others have shown that the IL-6 class cytokine oncostatin M (OSM) is an important regulator of bone remodeling. We hypothesize that simulated microgravity alters osteoblast OSM signaling, …

Effects Of Prey Abundance On Breeding Season Diet Of Northern Goshawks (Accipiter Gentilis) Within An Unusual Prey Landscape, Robert A. Miller, Jay D. Carlisle, Marc J. Bechard

Intermountain Bird Observatory Publications and Presentations

A critical element of diet analysis is species adaptability to alternative prey sources. The breeding-season diet of Northern Goshawks (Accipiter gentilis) includes both mammalian and avian species, varies geographically, and is often dependent upon tree squirrels of the genera Sciurus andTamiasciurus. We studied alternative prey sources of Northern Goshawks in the South Hills of south-central Idaho, an area where tree squirrels are naturally absent and other prey frequently important in the diet of goshawks, such as smaller corvids, are uncommon. We quantified the diet of goshawks using nest cameras and surveyed abundance of prey using line …

Bromus Tectorum Litter Alters Photosynthetic Characteristics Of Biological Soil Crusts From A Semiarid Shrubland, Marcelo D. Serpe, Eric Roberts, David J. Eldridge, Roger Rosentreter

Biology Faculty Publications and Presentations

Invasion by the exotic annual grass Bromus tectorum has increased the cover and connectivity of fine litter in the sagebrush steppes of western North America. This litter tends to cover biological soil crusts, which could affect their metabolism and growth. To investigate this possible phenomenon, biological soil crusts dominated by either the moss Bryum argenteum or the lichen Diploschistes muscorum were covered with B.tectorum litter (litter treatment) or left uncovered (control treatment) and exposed to natural field conditions. After periods of five and ten months, we removed the litter and compared the photosynthetic performance of biological soil crusts …

Minor Fibrillar Collagens, Variable Regions Alternative Splicing, Intrinsic Disorder, And Tyrosine Sulfation, Ming Fang, Reed B. Jacob, Owen Mcdougal, Julia Oxford

Biomolecular Research Center Publications and Presentations

Minor fibrillar collagen types V and XI, are those less abundant than the fibrillar collagens types I, II and III. The alpha chains share a high degree of similarity with respect to protein sequence in all domains except the variable region. Genomic variation and, in some cases, extensive alternative splicing contribute to the unique sequence characteristics of the variable region. While unique expression patterns in tissues exist, the functions and biological relevance of the variable regions have not been elucidated. In this review, we summarize the existing knowledge about expression patterns and biological functions of the collagen types V and …

Irruptive Migration Of Chestnut-Backed Chickadees To Southwestern Idaho, Jay D. Carlisle

Intermountain Bird Observatory Publications and Presentations

I document irruptive movements of the Chestnut-backed Chickadee to Lucky Peak in southwestern Idaho, over 80 km from its regular range. Chestnut-backed Chickadees were captured and/or observed at Lucky Peak in 2000, 2004, and 2008. To evaluate the context for this phenomenon, I also examined data on capture of all chickadees and other irruptive species at Lucky Peak and numbers of irruptive species recorded on Idaho Christmas Bird Counts (CBC) from 1997 to 2011. Though CBCs in the winter of 2004–05 (following the largest movement of Chestnut-backed Chickadees at Lucky Peak in fall 2004) found high numbers of many irruptive …