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Integrative Genomic And Transcriptomic Analysis For Pinpointing Recurrent Alterations Of Plant Homeodomain Genes And Their Clinical Significance In Breast Cancer, Huimei Yu, Yuanyuan Jiang, Lanxin Liu, Wenqi Shan, Xiaofang Chu, Zhe Yang, Zeng-Quan Yang

Oncology Faculty Publications

A wide range of the epigenetic effectors that regulate chromatin modification, gene expression, genomic stability, and DNA repair contain structurally conserved domains called plant homeodomain (PHD) fingers. Alternations of several PHD finger-containing proteins (PHFs) due to genomic amplification, mutations, deletions, and translocations have been linked directly to various types of cancer. However, little is known about the genomic landscape and the clinical significance of PHFs in breast cancer. Hence, we performed a large-scale genomic and transcriptomic analysis of 98 PHF genes in breast cancer using TCGA and METABRIC datasets and correlated the recurrent alterations with clinicopathological features and survival of …

Pengaruh Metode Uchikomi Dan Flexibility Terhadap Hasil Bantingan Pada Pejudo Daerah Istimewa Yogyakarta, Suwarli Suwarli

Jurnal Keolahragaan

Penelitian bertujuan mengetahui: (1) perbedaan pengaruh antara metode uchikomi dengan menggunakan karet ban sepeda dan uchikomi jalan terhadap hasil bantingan judo, (2) perbedaan hasil bantingan antara pejudo yang memiliki flexibility tinggi dan flexibility rendah, dan (3) interaksi antara metode uchikomi dan flexibility terhadap hasil bantingan judo. Metode penelitian adalah eksperimen dengan rancangan faktorial 2 x 2. Populasi penelitian adalah pejudo D.I. Yogyakarta yang berjumlah 44 pejudo. Sampel penelitian ini 24 pejudo yang diambil dengan teknik Purposive Rondom Sampling. Data penelitian ini diperoleh melalui tes dan pengukuran. Instrumen penelitian adalah sit and reach dan Randori (dipertandingkan) selama tiga menit. Teknik …

Pengaruh Latihan Imagery Dan Koordinasi Terhadap Keterampilan Forehand Drive Petenis Pemula, Risti Nurfadhila

Jurnal Keolahragaan

Penelitian ini bertujuan untuk mengetahui: (1) perbedaan pengaruh antara latihan imagery dengan menggunakan script dan video terhadap keterampilan forehand drive petenis pemula, (2) perbedaan keterampilan forehand drive antara petenis pemula yang memiliki koordinasi tinggi dan koordinasi rendah, (3) interaksi antara latihan imagery dan koordinasi terhadap keterampilan forehand drive petenis pemula. Metode penelitian adalah eksperimen dengan rancangan faktorial 2 x 2. Sampel penelitian adalah 32 orang petenis pemula mahasiswa PJKR FIK UNY yang mengambil mata kuliah olahraga pilihan tenis yang diambil dengan teknik Purposive Random Sampling. Seluruh data penelitian diperoleh melalui tes dan pengukuran terhadap koordinasi dengan menggunakan tes koordinasi …

Pengaruh Latihan Beban Dan Kekuatan Otot Terhadap Hypertrophy Otot Dan Ketebalan Lemak, Eko Sucipto, Widiyanto Widiyanto

Jurnal Keolahragaan

Penelitian ini bertujuan untuk mengetahui: (1) perbedaan pengaruh antara latihan beban metode compound set dan circuit training, (2) perbedaan pengaruh kekuatan otot tinggi dan rendah,dan (3) interaksi antara metode latihan beban dan kekuatan terhadap hasil hypertrophy otot dan ketebalan lemak. Populasi dalam penelitian ini adalah 56 members yang programnya hypertrophy otot dari Fitness Centre GOR FIK UNY dan Fitness Centre Club Arena Hotel Jayakarta Yogyakarta. Sampel yang berjumlah 28 members ditentukan dengan teknik Purposive Rondom Sampling. Teknik analisis data yang digunakan dalam penelitian ini adalah Analisis Varian (ANAVA). Hasil penelitian adalah: (1) ada perbedaan pengaruh yang signifikan antara …

Pengaruh Metode Latihan Dan Persepsi Kinestetik Terhadap Keterampilan Groundstrokes Tenis Lapangan Pada Siswa Sd, Suryono Suryono

Jurnal Keolahragaan

Penelitian ini bertujuan untuk mengetahui: (1) Perbedaan pengaruh antara metode demonstrasi dan metode audio visual terhadap keterampilan groundstrokes tenis lapangan, (2) Perbedaan pengaruh antara persepsi kinestetik terhadap keterampilan groundstrokes tenis lapangan, dan (3) interaksi metode latihan dan persepsi kinestetik terhadap keterampilan groundstrokes. Penelitian ini merupakan penelitian eksperimen. Populasi penelitian adalah siswa SD Negeri Petinggen dan SD Negeri Terbansari yang berjumlah 75 siswa. Sampel penelitian 40 siswa ditentukan dengan teknik purposive sampling. Pengumpulan data menggunakan tes persepsi kinestetik dan keterampilan groundstrokes. Instrumen dalam penelitian ini adalah horizontal liner space test dan memantulakan bola ke dinding. Teknik analisis data …

Minibrain And Wings Apart Control Organ Growth And Tissue Patterning Through Downregulation Of Capicua, Liu Yang

Graduate Doctoral Dissertations

The regulation of organ growth is a fundamental aspect of developmental biology. My work uses Drosophila as a model system to understand how the various growth regulators are coordinated. The transcriptional repressor Capicua (Cic) controls tissue patterning and restricts organ growth, and has been recently implicated in several cancers and neurodegenerative diseases. Cic has emerged as a primary sensor of signaling downstream of the receptor tyrosine kinase (RTK)/extracellular signal-regulated kinase (ERK) pathway, but how Cic activity is regulated in different cellular contexts remains poorly understood. In order to identify Cic regulators, I have used affinity purification/mass spectrometry (AP-MS) to study …

Evolution In The Deep Sea: Scales And Mechanisms Of Population Divergence, Amanda E. Glazier

Graduate Doctoral Dissertations

The deep sea is the Earth’s largest ecosystem and harbors a unique and largely endemic fauna. Although most research has focused on the ecological mechanisms that allow coexistence, recent studies have begun to investigate how this remarkable fauna evolved.. My work quantifies geographic patterns of genetic variation and investigates potential mechanisms that shape evolution in the deep ocean.

Bathymetric genetic divergence is common in the deep sea with population structure typically decreasing with depth. The evolutionary mechanisms that underlie these patterns are poorly understood. Geographic patterns of genetic variation indicated that the protobranch bivalve Neilonella salicensis was composed of two …

Variants In Wfs1 And Other Mendelian Deafness Genes Are Associated With Cisplatin-Associated Ototoxicity, Heather E. Wheeler, Eric R. Gamazon, Robert Frisina, Carlos Perez-Cervantes, Omar El Charif, Brandon Mapes, Sophie D. Fossa, Darren Feldman, Robert Hamilton, David J. Vaughn, Clair Beard, Chunkit Fung, Chiristian Kollmannsberger, Jeri Kim, Taisei Mushiroda, Michiaki Kubo, Shirin Ardeshir-Rouhani-Fard, Lawrence H. Einhorn, Nancy Cox, M. Eileen Dolan, Lois Travis

Bioinformatics Faculty Publications

Cisplatin is one of the most commonly used chemotherapy drugs worldwide and one of the most ototoxic. We sought to identify genetic variants that modulate cisplatin-associated ototoxicity (CAO). Experimental Design: We performed a genome-wide association study (GWAS) of CAO using quantitative audiometry (4-12 kHz) in 511 testicular cancer survivors of European genetic ancestry. We performed polygenic modeling and functional analyses using a variety of publicly available databases. We used an electronic health record cohort to replicate our top mechanistic finding. Results: One SNP, rs62283056, in the first intron of Mendelian deafness gene WFS1 (wolframin ER transmembrane glycoprotein) and an expression …

Analyzing And Modeling The Dysfunction Of Inhibitory Neurons In Alzheimer’S Disease, Carlos Perez, Jokubas Ziburkus, Ghamim Ullah

Physics Faculty Publications

Alzheimer’s disease (AD) is characterized by the abnormal proteolytic processing of amyloid precursor protein, resulting in increased production of a self-aggregating form of beta amyloid (Aβ). Several lines of work on AD patients and transgenic mice with high Aβ levels exhibit altered rhythmicity, aberrant neuronal network activity and hyperexcitability reflected in clusters of hyperactive neurons, and spontaneous epileptic activity. Recent studies highlight that abnormal accumulation of Aβ changes intrinsic properties of inhibitory neurons, which is one of the main reasons underlying the impaired network activity. However, specific cellular mechanisms leading to interneuronal dysfunction are not completely …

Evolutionary Dynamics Of Tree Invasions: Complementing The Unified Framework For Biological Invasions, Rafael D. Zenni, Ian A. Dickie, Michael J. Wingfield, Heidi Hirsch, Casparus J. Crous, Laura A. Meyerson, Treena I. Burgess, Thalita G. Zimmermann, Metha M. Klock, Evan Siemann, Alexandra Erfmeier, Roxana Aragon, Lia Montti, Johannes J. Le Roux

Faculty Publications, Environmental Studies

Evolutionary processes greatly impact the outcomes of biological invasions. An extensive body of research suggests that invasive populations often undergo phenotypic and ecological divergence from their native sources. Evolution also operates at different and distinct stages during the invasion process. Thus, it is important to incorporate evolutionary change into frameworks of biological invasions because it allows us to conceptualize how these processes may facilitate or hinder invasion success. Here, we review such processes, with an emphasis on tree invasions, and place them in the context of the unified framework for biological invasions. The processes and mechanisms described are pre-introduction evolutionary …

Winner's Curse Correction And Variable Thresholding Improve Performance Of Polygenic Risk Modeling Based On Genome-Wide Association Study Summary-Level Data, Jianxin Shi, Ju-Hyun Park, Jubao Duan, Sonja T. Berndt, Winton Moy, Kai Yu, Lei Song, William Wheeler, Xing Hua, Debra Silverman, Montserrat Garcia-Closas, Chao Agnes Hsiung, Jonine D. Figueroa, Victoria K. Cortessis, Nuria Malats, Margaret R. Karagas

Dartmouth Scholarship

Recent heritability analyses have indicated that genome-wide association studies (GWAS) have the potential to improve genetic risk prediction for complex diseases based on polygenic risk score (PRS), a simple modelling technique that can be implemented using summary-level data from the discovery samples. We herein propose modifications to improve the performance of PRS. We introduce threshold-dependent winner’s-curse adjustments for marginal association coefficients that are used to weight the single-nucleotide polymorphisms (SNPs) in PRS. Further, as a way to incorporate external functional/annotation knowledge that could identify subsets of SNPs highly enriched for associations, we propose variable thresholds for SNPs selection. We applied …

Stability Of Cross-Feeding Polymorphisms In Microbial Communities, Ivana Gudelj, Margie Kinnersley, Peter Rashkov, Karen Schmidt, Frank Rosenzweig

Biological Sciences Faculty Publications

Cross-feeding, a relationship wherein one organism consumes metabolites excreted by another, is a ubiquitous feature of natural and clinically-relevant microbial communities and could be a key factor promoting diversity in extreme and/or nutrient-poor environments. However, it remains unclear how readily cross-feeding interactions form, and therefore our ability to predict their emergence is limited. In this paper we developed a mathematical model parameterized using data from the biochemistry and ecology of an E. coli cross-feeding laboratory system. The model accurately captures short-term dynamics of the two competitors that have been observed empirically and we use it to systematically explore the stability …

Influence Of Maternal Breast Milk Ingestion On Acquisition Of The Intestinal Microbiome In Preterm Infants., Katherine E Gregory, Buck S Samuel, Pearl Houghteling, Guru Shan, Frederick M Ausubel, Ruslan I Sadreyev, W Allan Walker

Faculty Publications

BACKGROUND: The initial acquisition and early development of the intestinal microbiome during infancy are important to human health across the lifespan. Mode of birth, antibiotic administration, environment of care, and nutrition have all been shown to play a role in the assembly of the intestinal microbiome during early life. For preterm infants, who are disproportionately at risk of inflammatory intestinal disease (i.e., necrotizing enterocolitis), a unique set of clinical factors influence the establishment of the microbiome. The purpose of this study was to establish the influence of nutritional exposures on the intestinal microbiome in a cohort of preterm infants early …

Exploring Knowledge, Beliefs And Practices Of Radon Gas Exposure Among Public Health Workers, Paschal Nwako

Seton Hall University Dissertations and Theses (ETDs)

Radon gas exposure is the highest cause of lung cancer among people that never smoked in the United States. People exposed to elevated levels of radon had a higher risk of developing lung cancer. Achieving the long term goal of the Indoor Radon Abatement Act (IRAA) of 1988 require a combined efforts of government agencies. Public health workers are change agents and their role in protecting and improving the health of their communities are well documented. This study created and utilized a multi question survey, the Public Health Workers Radon Assessment Instrument (PHWRAI) to explore knowledge, beliefs, personal and professional …

Cullin-4 Regulates Wingless And Jnk Signaling-Mediated Cell Death In The Drosophila Eye., Meghana Tare, Ankita Sarkar, Shimpi Bedi, Madhuri Kango-Singh, Amit Singh

Biology Faculty Publications

In all multicellular organisms, the fundamental processes of cell proliferation and cell death are crucial for growth regulation during organogenesis. Strict regulation of cell death is important to maintain tissue homeostasis by affecting processes like regulation of cell number, and elimination of unwanted/unfit cells. The developing Drosophila eye is a versatile model to study patterning and growth, where complex signaling pathways regulate growth and cell survival. However, the molecular mechanisms underlying regulation of these processes is not fully understood. In a gain-of-function screen, we found that misexpression of cullin-4 (cul-4), an ubiquitin ligase, can rescue reduced eye mutant phenotypes. Previously, …

Connecting Culturally And Spiritually To Healthy Eating: A Community Assessment With Native Hawaiians, Mary Frances Oneha, Joan Dodgson, Mabel Ho`Oipo Decambra, Carol Titcomb, Rachelle Enos, Sandie Morimoto-Ching

Asian/Pacific Island Nursing Journal

Many of the chronic illnesses disproportionately experienced by Native Hawaiians are directly related to poor diets and long-standing obesity beginning in childhood. We report on the findings of in-depth key informant interviews (N = 14) that took place in two Native Hawaiian communities as part of a larger, community-based participatory research study that included a community assessment through individual interviews and focused group discussions, and a pilot intervention targeting pregnant women, their infants, and families. Four categories emerged from the qualitative analysis of interview transcripts that described an understanding of “healthy eating”: family roles and responsibilities, aspects of community …

Expansion Of The Molecular And Morphological Diversity Of Acanthamoebidae (Centramoebida, Amoebozoa) And Identification Of A Novel Life Cycle Type Within The Group, Alexander Tice, Lora Shadwick, Anna Maria Fiore-Donno, Stefen Geisen, Seungho Kang, Gabriel Schuler, Frederick Spiegel, Katherine Wilkinson, Michael Bonkowski, Kenneth Dumack, Daniel Lahr, Eckhard Voelcker, Steffen Claub, Junling Zhang, Matthew Brown

Faculty Publications, Biological Sciences

Background Acanthamoebidae is a “family” level amoebozoan group composed of the genera Acanthamoeba, Protacanthamoeba, and very recently Luapeleamoeba. This clade of amoebozoans has received considerable attention from the broader scientific community as Acanthamoeba spp. represent both model organisms and human pathogens. While the classical composition of the group (Acanthamoeba + Protacanthamoeba) has been well accepted due to the morphological and ultrastructural similarities of its members, the Acanthamoebidae has never been highly statistically supported in single gene phylogenetic reconstructions of Amoebozoa either by maximum likelihood (ML) or Bayesian analyses. Results Here we show using a phylogenomic approach that the Acanthamoebidae is …

Cytochrome C Can Form A Well-Defined Binding Pocket For Hydrocarbons, Levi J. Mcclelland, Harmen B. Steele, Frank G. Whitby, Tung-Chung Mou, David Holley, J B. Ross, Stephen R. Sprang, Bruce E. Bowler

Biological Sciences Faculty Publications

Cytochrome c can acquire peroxidase activity when it binds to cardiolipin in mitochondrial membranes. The resulting oxygenation of cardiolipin by cytochrome c provides an early signal for the onset of apoptosis. The structure of this enzyme-substrate complex is a matter of considerable debate. We present three structures at 1.7-2.0 Å resolution of a domain-swapped dimer of yeast iso-1-cytochrome c with the detergents, CYMAL-5, CYMAL-6, and ω-undecylenyl-β-d-maltopyranoside, bound in a channel that places the hydrocarbon moieties of these detergents next to the heme. The heme is poised for peroxidase activity with water bound in place of Met80, which serves as the …

Optimization On Extraction Of Capsaicinoids Assisted By Ultrasonic-Microwave Synergistic Extracting, Pang Min, Wang Yuanhui, Liu Qian

Food and Machinery

Paprikas were soaked in 90% (V/V) ethanol to extract capsaicinoids by using ultrasonic and microwave assisted extraction (UMAE). Based on the results of single factor experiments, the optimized extraction condition were gotten with orthogonal tests: the ultrasonic power of 50 W, the microwave power of 240 W, the solid- solvent ratio of 1∶20 (g/mL), the microwave time of 480 s, the paprikas particle size of 80 mesh, and the extracting time twice. The present study obtained an extraction rate of 84.52%. Compared with the conventional water bath extraction method, UMAE has the advantages of shorter extraction time and higher efficiency, …

Tree Circumference Dynamics In Four Forests Characterized Using Automated Dendrometer Bands, Valentine Herrmann, Sean M. Mcmahon, Matteo Detto, James A. Lutz, Chia-Hao Chang-Yang, Kristina J. Anderson-Teixeira

Wildland Resources Faculty Publications

Stem diameter is one of the most commonly measured attributes of trees, forming the foundation of forest censuses and monitoring. Changes in tree stem circumference include both irreversible woody stem growth and reversible circumference changes related to water status, yet these fine-scale dynamics are rarely leveraged to understand forest ecophysiology and typically ignored in plot- or stand-scale estimates of tree growth and forest productivity. Here, we deployed automated dendrometer bands on 12–40 trees at four different forested sites—two temperate broadleaf deciduous, one temperate conifer, and one tropical broad-leaf semi-deciduous—to understand how tree circumference varies on time scales of hours to …

Binding And Interaction Effect Of Three Kinds Of Plant Extracts On Glycolipid Metabolism In Type Ⅱ Diabetic Mice, Zhang Jiyuan, Liu Jinfu, Xiao Ping, Liu Zixuan

Food and Machinery

In this paper, the type II diabetes model was established and the optimal dose of the pumpkin polysaccharide, pueraria flavonoids and pu-erh theabrowns were determined by measured blood glucose. On the basis, the fasting blood glucose (FBG), insulin (INS), insulin resistance (IR), high density lipoprotein (HDL) and low density lipoprotein (LDL) were also investigated. The FBG, INS, IR, HDL, LDL combined group was significantly better than the single component (P＜0.05). The results revealed that the combinations of pumpkin polysaccharide and pu-erh theabrowns, pueraria flavonoids and pu-erh theabrowns were effective. However, the pumpkin polysaccharide factorial analyses showed that the interactions of …

The Batch Fermentation Kinetics Of Mulberry Fruit Wine, Hu Yongzheng, Li Mingyuan, Li Mei, Ding Wenwu, Kang Ling, Wang Chong, Chen Jianhua, Li Chunmei

Food and Machinery

The laws of cell growth, product formation and substrate consumption during mulberry wine fermentation were studied with mulberry juice. The kinetic models were established through Logistic model equation to fit the curves of cell growth, product formation and substrate consumption. The results showed as follows: When the initial sugar concentration was 200 g/L, the inoculation of activated yeast was 5%, and the fermentation time lasted for 100 h at 28 ℃, the residual sugar concentration of fermentation broth decreased to 3.86 g/L, the number of yeast increased to 2.83×10⁸ CFU/mL and the ethanol concentration reached 9.01%. The models fitted …

Effect Of Ph Value In Preparation Process On Functional Properties Of Gelatin From Bighead Carp (Hypophthalmichthys Nobilis) Scale, Sha Xiaomei, Tu Zongcai, Wand Hui, Zhang Lu, Huang Tao, Hu Zizi

Food and Machinery

The effect of pH value in preparation process on proximate composition, yield and functional properties including gel strength, melting temperature and turbidity of gelatin from bighead carp (Hypophthalmichthys nobilis) scale was investigated. Moreover, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to evaluate the change of molecular weight distribution for fish gelatin. The results indicated that regardless of pH values in the range of 3.0~7.0 for preparation process, fish gelatin had a high quality with high protein content and low ash content. With pH value increasing, the yield of fish gelatin gradually decreased, however, turbidity showed an increased trend. When …

Study On Acidizing Conditions Of Acid-Induced Surimi Gel And Analysis Of Gel Properties, Wu Xuewei, Jiang Qixing, Xu Yanshun, Yu Peipei, Xia Wenshui

Food and Machinery

This research investigated the influence of setting temperature and time on the gel properties of glucono-δ-lactone (GDL)-induced surimi gel. The results showed that the acidification rate increased with the temperature increasing from 30 ℃ to 50 ℃. Consequently, the time for the gel pH to stabilize decreased which induce to the decline of gel strength and the increase of the expressible water content of GDL-induced surimi gel. It is indicated that protein subunits solubilized in various solvents revealed that the formation of GDL-induced surimi gel mainly through hydrophobic interactions and it increased with temperature. The content of total SH increased …

Studies On Formation Of Ages By Lysine In Maillard Reaction, Li Pu, Xia Qiuqin, Liu Guimei, Lu Yongling, Lu Lishuang

Food and Machinery

[Purpose] The different influencing factors are investigated on producing AGEs by Lysine in Maillard reaction, and the different between single amino acid (Lysine, or Argine) -reducing and mixed Lys- Arg -reducing sugar system are compared. [Method] Fluorescence spectrometry (λex / λem =370 / 440 nm) is used to detect the amount of AGEs, the factors of sort and concentration of sugar, metal ion, temperature, pH and genistein concentration in Maillard reaction and proportion of lysine and arginine in Lys/Arg-reducing sugar system was discussed. [Result] The increasing of sugar concentration, pH, or temperature, the more amounts of AGEs was produced. Fe …

A Slic-Basedsuperpixel Segmentation Method By Using Local Texture Features For Granular Image, Li Xiaoyu, Zhang Qiuju

Food and Machinery

This paper adopts SLIC-based superpixel segmentation method in the granular image detection. SLIC method segments the granular image into superpixel block which will reduce the complexity of the subsequent image processing. As SLIC superpixel segmentation method doesn’t use the texture feature in the distance calculation, the detail of the outline for the granular object is lost. This paper adopts the CRLBP local texture operator as the texture feature to improve the SLIC segmentation’s distance calculation and searches the similar pixel in circle neighborhood pixels to guarantee the processing speed. The test on cotton seed image shows that the improved SLIC …

Quality Assessment Of Apple Chips Based On Data Fusion Processing And Nirs Method, Guan Xiao, Rao Li, Liu Jing, Kuang Jingyun

Food and Machinery

In this study, five quality indicators, i.e. moisture, soluble solid content, total sugar content, titratable acid content and hardness, of apple chips during processing period were analyzed by analytic hierarchy process, and quality scores was obtained. Near infrared spectroscopy (NIRS) combined with partial least-squares discriminant analysis (PLS-DA) was applied for the prediction of quality scores of apple chips. The results showed that the correlation coefficients between predicted category variable of calibration and the measured category variable were 0.84, 0.63 and 0.89, and the root mean square error of cross validation were 0.26, 0.34 and 0.22 for each quality category, respectively. …

Research Of Volatile Components Of Lanzhou Fat-Tailed Sheep, Li Zhenzi, Yang Jutian, Qi Gaozhan, Zang Rongxin, Liu Hongna, Long Ling, Cao Xin

Food and Machinery

It was aimed to analyze the volatile components and key volatile components of Lanzhou fat-tailed sheep in different gender in this study, and the small fat-tail sheep were chosen as control. The results showed that Lanzhou fat-tailed wethers contained totally 80 kinds of volatile materials, while ewes had 85 kinds. Aldehydes were found the key volatile components, and the relative contents were respectively 31.110% and 22.305%. The key volatile components contained decanal, octanal, nonanal, 2, 4-nonadienal, 2-nonenal, 2,4-decadienal and (E)-2-decenal, which smelled like wax, fat, mushroom and cucumber, and wethers contained 2 more kinds aldehydes than ewes. Compared to small …

Study On Variation Regularity And Characteristics Of Lycium Barbarum Polysaccharide In Different Mature Stage, Duan Yue, Liu Dunhua, Li Shiyao, Zhao Yuhui, Fang Xiang

Food and Machinery

Studied the change of Lycium barbarum polysaccharides (LBP) in different growth periods, by using the wolfberries in different growth periods as research objects, and analyzed the LBP composition by HPLC. Taking VC as the control sample, the LBP’s activities of scavenging hydroxyl radicals, superoxide anions, DPPH and ABTS were analyzed and compared in different growth periods. The rheological characteristics of LBP in different growth periods were analyzed by a rheometer. The results indicated that the monosaccharide composition of LBP was the same in different growth periods, but the monosaccharide molar ratios of the polysaccharides were significantly different. The antioxidant activity …

Identification Of A Strain Of High Yieldacetic Acid Bacteria From Ferment And Its Acid-Producing Characteristics, Lu Mengyao, Liu Shuliang, Hu Kaidi, Zhang Aiqing

Food and Machinery

A strain of high yield acetic acid bacteria LMY-1 was isolated from 10 kinds of ferment samples by isolation and purification, the acid qualitative and quantitative screen for producing. The strain was identified as Acetobacter cibinongensis by analysis of morphology, physiological and biochemical indicators and identification of 16S rDNA. The growth and acid-producing characteristics of strain LMY-1 was studied by fruit and vegetable culture medium with apple, pear and cucumber. Results, the optimum acid temperature for strain LMY-1 was 30 ℃； In 4 mL/100 mL ethanol content, strain LMY-1 produced the highest acid amount (22.72 g/L), higher than strain CICC7015(Acetobacter …