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Characterization Of De Novo Fatty Acid Biosynthesis In Soybean Somatic Embryo Plastids, Karen Clark
Characterization Of De Novo Fatty Acid Biosynthesis In Soybean Somatic Embryo Plastids, Karen Clark
All Theses
A method for the isolation of intact physiologically active plastids from rapidly developing soybean (Glycine max L.) somatic embryos has been developed for the in vitro study of lipid metabolism. Using de novo fatty acid biosynthesis from 14C-acetate as a marker for physiological functionality, the greatest rates of fatty acid biosynthesis were recovered in 3000 x g fractions that were isolated in the presence of 0.5 M sorbitol, with essentially no activity occurring in the 3000 x g supernatant. Plastids purified on 10% Percoll were approximately 70 and 97 % free from mitochondrial and ER contamination, respectively, as judged …
Simplicillium Lanosoniveum, A Mycoparasite Of Phakopsora Pachyrhizi And Its Use As A Biological Control Agent, Nicole A. Ward
Simplicillium Lanosoniveum, A Mycoparasite Of Phakopsora Pachyrhizi And Its Use As A Biological Control Agent, Nicole A. Ward
LSU Doctoral Dissertations
In 2007, a filamentous fungus was recovered from sori of soybean rust (SBR), caused by Phakopsora pachyrhizi, collected from Louisiana and Florida. This fungus was identified as Simplicillium lanosoniveum on the basis of ITS sequence data and morphological traits. Simplicillium lanosoniveum was found coiling within sori and around urediniospores and showed a trophic attraction to rust sori, extending from sorus to sorus. In co-inoculated soybean leaves, the fungus did not grow or establish on leaf surfaces until sori erupted. Similarly, S. lanosoniveum colonized within 3 days and sporulated within 4 days on leaves showing disease symptoms. In field studies, when …