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Channel Catfish, Ictalurus Punctatus, T-Lymphocyte Mitogenic Responses To Outer Membrane Proteins And Lps Of The Gram Negative Rod Bacterium, Edwardsiella Ictaluri, Barry Revis Hamilton

Morehead State Theses and Dissertations

A thesis presented to the faculty of the Department of Biological and Environmental Sciences at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Barry Revis Hamilton on December 8, 1995.

Fiber Source Characteristics And The Effects Of Recycling On Bod And Cod Effluent Levels In Corrugated Boxboard, Craig Mefford

Paper Engineering Senior Theses

The topic of waste loading for recycle paper mills is an important issue to our industry today. As of last year the EPA has proposed the cluster rule, which suggests that the BOD (Biochemical Oxygen Demand) and COD (Chemical Oxygen Demand) loading limits are being lowered encouraging use of lower effluent loading material. The main purposes of this thesis was to focus on the effects that recycling Korean Boxboard, American Boxboard, and high yield SW Kraft has on raw waste loading in a boardmill and to analyze the characteristics of these recycled materials. This will be accomplished by separating our …

The Effects Of A Surfactant On The Pulp Properties On Non-Sulfur Semichemical Pulps, Tim Schemanski

Paper Engineering Senior Theses

Semichemical pulping involves both mechanical and chemical pulping techniques. In semichemical pulping the liquor is in contact with the chips for a relatively short period of time. The faster with liquor can penetrate the chips before they see mechanical action, the better the pulp properties. The effects of adding a surfactant to the liquor was studied. It was found that the anionic ethoxylated surfactant which was added at the addition rate of 5#/ton to non-sulfur semi-chemical pulping liquor increased the lignin content of the resulting pulp and lowered the tensile strength.

The Effect Of Internally-Filled Pulp On Recycling, Anne Spruit

Paper Engineering Senior Theses

Recycling is an area of interest for much of the industry because of all the environmental concerns with the cutting down of trees, and the pulping operations to make paper. However, virgin fibers are still desired because they make stronger paper. Virgin fibers have a higher swelling capability than recycled fibers, that accounts for better bonding. Paper's physical properties also decrease rapidly as filler is substituted for fiber. Filler impairs interfiber bonding by preventing close contact with fibers. Filler is added to paper to increase opacity and brightness. However, there is a poor retention of filler in the sheet during …

The Effect Of Viscosity And Polymer Additives On Fluorescent Dyes In Latex Coatings, Mark P. Comensoli

Paper Engineering Senior Theses

Fluorescent Whitening Agents (FWAs) are commonly used in many areas of the pulp and paper industry. FWAs are also known as Optical Brightening Agents (OBAs) and generically as fluorescent dyes. Nearly one-third of all FWAs manufactured are used in the pulp and paper industry. FWAs work by converting ultraviolet light into blue-white light in the visible region. When FWAs are used in coatings with latex binders, the fluorescent dyes have a tendency to migrate toward the basesheet during drying. This is due to mainly to the lack of suitable carrier for the dye. Previous research has shown that the addition …

Characterization Of Effluent From Dioxirane Bleaching Sequences, Holly L. Dunnill

Paper Engineering Senior Theses

Pulp bleaching is a multi-staged process which uses different chemicals and conditions in each stage, with washing performed between these stages. With chlorine compounds being the most widely used bleaching agents, the water from bleaching and washing stages subsequently contains a high chloride content as well as chloro-organic compounds which makes them incompatible with pulping chemical recovery processes. They are, therefore, discharged as wastewater. This leads to the need for regulations regarding the effluent quality of the wastewater. The purpose of this study is to compare effluents from various dimethyl dioxirane bleaching sequences with those of commonly used industry bleaching …

Biological Determinism And The Human Genome Project: Is The Past Prologue?, Alexandra Dawn Breen

Theses & Honors Papers

In 1989, The National Institutes of Health, in collaboration with the United States Department of Energy, launched a three billion dollar project that could potentially change the way we view human life. The purpose of the project is to map every gene on every chromosome of the human DNA. The Human Genome Project, as it is referred to, is scheduled for completion by the year 2005. The frank reality of the Human Genome Project is that it will indeed offer some real and measurable benefit to different types of people, including some of those inflicted with fatal diseases and disorders; …

Regulation Of Leukotriene C4 Biosynthesis In Calcium Ionphore A23187-Challeneged August Rat Peritoneal Leukocytes, James M. Huebner

Masters Theses

Leukotriene (LT) C₄ is a potent myotropic product of arachidonic acid 5-lipoxygenation that is thought to play a role in the pathophysiology of immediate hypersensitivity reactions and inflammation. We designed the present study to evaluate the capacity of August (AUG) rat mixed peritoneal leukocytes to generate LTC₄ when challenged with different concentrations of the Ca²⁺ ionophore A23187 and to test the hypothesis that LTC₄ biosynthesis is regulated via a protein kinase C (PKC)-dependent mechanism. The AUG rat, which has a spontaneously high number of peritoneal eosinophils that can be harvested by a simple lavage procedure, is …

Morphometric, Isozyme And Random Amplified Polymorphic Dna (Rapd) : Analysis Of Sarotherodon Mossambicus Populations In Java, Indonesia, Yoedono Sovyanhadi

Loma Linda University Electronic Theses, Dissertations & Projects

The tilapia fish Sarotherodon mossambicus (Teleostei, Cichlidae) is an important protein source for Indonesians, is a strong competitor with some endemic species and .readily hybridizes with other tilapias. A base line assessment of the present genetic diversity of these populations in Indonesia was conducted to facilitate the management of this species. This study compared the effectiveness of the 3 techniques (morphometric, isozyme and RAPD) in revealing differences within and among 9 Javanese populations. The data were also used to estimate the rate of divergence among the populations.

Morphometric analysis showed significant size and shape differences among the populations. Isozyme and …

Reverse Osmosis Treatment Of Deinking Effluents, Lei Zhao

Masters Theses

The application of the reverse osmosis process for treatment of preclarified deinking effluent was studied for its efficiency and capacity to remove the total suspended and dissolved solids. Seven coagulants and flocculents were studied in the pretreatment to determine the best coagulant. Two variables, temperature and coagulant dose, were studied to determine the optimum conditions. For the reverse osmosis treatment, two types of thin film composite membranes, sea water and brackish water were used in the research. A set of orthogonal experiments and regression analyses were conducted to study the effects of pressure, temperature, feed concentration, and feed pH on …

Incidence And Life History Of Beech Scale, Initiator Of Beech Bark Disease, In The Great Smoky Mountains National Park, Robert Angelo Vance

Masters Theses

American beech, Fagus grandifolia Ehrlich, in eastern North America are currently threatened by the devastating beech bark disease. This disease is caused by the fungal pathogens, Nectria coccinea var. faginata Lohman, Watson and Ayers, and Nectria galligena Bres., which infect trees that are predisposed by infestations of beech scale, Cryptococcus fagisuga Lindinger. In 1993, beech bark disease was discovered in the Great Smoky Mountains National Park (GSMNP).

In 1994, a 2-year cooperative research project involving the National Park Service and the University of Tennessee Agricultural Experiment Station was initiated to study several aspects of beech bark disease in the GSMNP. …

Identification Of An Insulin-Responsive Element In The Rat Glucose-6-Phosphate Dehydrogenase Gene, Sheri Lynn Holmen

Masters Theses

The expression of several genes has been shown to be either positively or negatively regulated by insulin at the transcriptional level, however no single insulin responsive DNA element has yet been identified. The transcription factors responsible for stimulation or inhibition of gene expression by insulin also remain to be identified. In recent years, expression of the gene for glucose-6-phosphate dehydrogenase (G6PDH) in primary rat hepatocytes has been found to be influenced by hormones such as insulin, dexamethosone, and glucagon. The molecular mechanism(s) however, underlying hormonal regulation of G6PDH gene transcription remains to be elucidated. The focus of this study was …

Data On Gait Characteristics Of Four, Five, And Six Year Old Children Using Three Dimensional Video Motion Analysis, Donna Soave Nichols

Rehabilitation Sciences Theses & Dissertations

The purpose of this study resulted from a need to gather and analyze data on gait characteristics of normal children using the Peak Performance Technologies, Incorporated system, initiate a data base for the Motion Analysis Center at Old Dominion University and compare a computerized motion analysis system to studies using different methods. Fifteen children, aged four to six, were evaluated during gait using a video-based computerized motion analysis system manufactured by Peak Performance Technologies, Incorporated. The gait characteristics studied were stride length, cadence, angular displacements and angular velocities of the pelvis, hips, knees and ankles. The results showed that methods …

Active Inhibition Of T Cell Proliferation By Monocytes, Dean Anthony Lee

Loma Linda University Electronic Theses, Dissertations & Projects

Monoclonal antibodies (mAb) to MHC class II molecules (anti-class II mAb) have been well described in their ability to inhibit T cell activation. The most current model is that anti-class II mAb interfere directly with the T cell receptor-major histocompatibility complex crosslinking which is necessary for T cell activation. The anti-CD3 mAb, OKT3, is an MHC class II molecule-independent mitogen which causes T cell activation by direct ligation of the TCR-CD3 complex. OKT3 and a panel of anti-class II mAb were used to dissect the individual roles played by these two receptors in activation and inhibition of peripheral blood mononuclear …

Effect Of Myristicin, A Constituent Of Parsley, On Levels Of Glutathione And Glutathione S-Transferase In Mice Tissues, Maria Theresa Tijerina

Theses and Dissertations - UTB/UTPA

Glutathione (GSH) and related enzymes interact as a ubiquitous and efficient detoxification system in biological organisms. Several naturally occurring, as well as synthetic compounds, are known to increase the levels of glutathione/glutathione S-transferases (GST). One of the natural compounds includes myristicin, which is a constituent of parsley leaf oil as well as other natural foods. The extent to which the levels of the GSH/GST system can be induced appears to be dependent on the specific tissue type and the amount of myristicin administered. Our results show that increased amounts of myristicin exhibited beneficial effects within a certain range for each …

Feeding And Nesting Ecology Of The Great Kiskadee, Pitangus Sulphuratus Texanus (Passeriformes: Tyrannidae) In The Lower Rio Grande Valley, Texas, Robert Luis Gorena

Theses and Dissertations - UTB/UTPA

The foraging and nesting ecology of the Great Kiskadee was studied in the Lower Rio Grande Valley, Texas during 1994 and 1995. Adult kiskadees were found to be omnivorous, feeding mainly on fleshy fruits, fish, and dragonflies, and capturing animal prey mainly by surface gleaning into water or hawking prey out of midair. Nests are constructed mainly during March and April and are built at heights between 6 and 10 meters apparently without preference to tree species. Of 29 nests observed, 66% (19) were active during at least part of the breeding season. Nestlings are fed a diet very similar …

An Analysis Of 1994 Air Force Toxic Release Inventory Reporting, Brian A. Pollock

Theses and Dissertations

The purpose of this research is to analyze the Air Force's 1994 Toxic Release Inventory data. Statistical tests were used to meet the following research objectives: (1) review the CY 94 data to determine which chemicals were most often reported and which bases and major commands had the most releases in terms of the number of chemicals reported and the amount of chemicals reported and (2) investigate factors which could influence the reporting status of a facility. An analysis of the TRI data showed that dichloromethane was the chemical released in the largest amount while methyl ethyl ketone was the …

The Aerobic Metabolism And Behavior Of The Burrow-Dwelling Mantis Shrimp, Hemisquilla Ensigera Californiensis, Jason Jospeh Cassista

Loma Linda University Electronic Theses, Dissertations & Projects

The predatory stomatopod Hemisquilla ensigera californiensiswas captured at 10-20 m depths off California and transferred to an observation tank with artificial burrows similar to those observed in the field. The animals were maintained under a 1OL:140 light-dark cycle and monitored on a 24-hour basis using a time-lapse video recorder. Infrared lamps were used for observations within the burrow and at night. Metabolic rates, as a function of oxygen tension, were measured in respirometry chambers using polarographic oxygen electrodes.

This species is a near complete oxyconformer, so aerobic metabolism is strongly depressed at low oxygen levels, yet the animals remained …

Modified Model For Transient Response Of Mrna Synthesis To Magnetic Field Exposure, Timothy W. Crosnoe

Theses and Dissertations

It is a well-known fact that high frequency, or radio frequency, radiation can be directly harmful to biological tissue. The radiation frequencies to which humans are most exposed, however, are the extremely low frequencies, or ELFs. To date there has been no definitive measure for predicting the ability of low frequency electromagnetic radiation to cause adverse biological effects. Two specific measures, average magnetic field and kinetic index, have been used in studies to determine if they are useful predictors of adverse effects, specifically leukemia. Unfortunately, both have shown only marginal results. This thesis analyzed and improved the kinetic index measure …

Crop Tree Management Of Upland Hardwoods In West Tennessee, David O'Neal

Masters Theses

A 40-year-old upland hardwood stand in west Tennessee was selected to test the effectiveness of crop tree management techniques. The stand consisted of 20 acres with each acre having the potential to contain 36 crop trees. Treatments consisted of: 1) a crown release, 2) fertilization (150 lbs. N and 35 lbs. P₂0₅ per acre) and 3) a combined release and fertilizer treatment.

A severe ice storm struck west Tennessee the winter following the study's initiation. Results indicated that release greatly increased a crop tree's susceptibility to severe ice damage and that black cherry (Prunus serotina Ehrh.) …

Foodservice Conditions In Licensed Urban And Rural Child Care Centers: An Application Of The National Health And Safety Performance Standards, Todd Michael Kirkpatrick

Masters Theses

Objectives: To assess the degree to which child care centers in urban and rural counties met foodservice standards as documented in the National Health and Safety Performance Standards; to determine if a difference in foodservice conditions existed between these two groups; to determine if the foodservice conditions at child care centers are related to the directors' perception of existing foodservice conditions or the importance of maintaining safe foodservice conditions, or previous environmental health inspection scores; to identify child care staffing needs.

Subjects: 36 urban and 34 rural child care centers in east Tennessee

Design: On-site assessment …

An Assessment Of Nutrition Policies And Practices In Licensed Child Care Agencies In Tennessee, Nicole Anne Cunningham

Masters Theses

Objective Research was conducted to determine the extent to which a sample of licensed child care agencies in Tennessee met 31 nutrition and food service standards as contained in the National Health and Safety Performance Standards: Guidelines for Out-of-Home Child Care Programs and to identify any significant differences by type of agency, USDA Child and Adult Care Food Program (CACFP) participation, and profit status.

Design A random sample of 300 licensed child care agencies stratified by type was surveyed by mailed questionnaire composed of 59 open and closed-ended items and five-point Likert-like scaled items. Three primary scores were calculated to …

Gis Modeling Of Forest Cover Distributions In A Southern Appalachian Watershed, Daniel Thomas Rutledge

Masters Theses

This study develops a method to model forest cover distributions in the Little Tennessee River Watershed in Macon County, North Carolina using a geographic information system (GIS). The model employs common environmental variables, e.g. elevation, aspect, and slope, and a newly developed metric called a topographic index. The topographic index quantifies landscape form to reflect the degree of site convexity or concavity. To generate index values, the GIS sums relative aspect differences between all adjacent cells and the center cell in a neighborhood analysis. To determine predominant community types and their environmental ranges in the study area, I surveyed canopy …

Effects Of Ammonium Perchlorate On The Thyroid Hormone Levels Of The Sprague-Dawley Rat, James H. King Jr.

Theses and Dissertations

The purpose of this research was to determine the threshold dose for ammonium perchlorate (AP) in the Sprague-Dawley rat. No dose response data exist for AP and the EPA has studied literature on the subject of perchlorates to determine a provisional reference dose. The Perchiorate Group, a consortium of DoD and industry representatives, believes this provisional reference dose is too conservative. This experiment was executed to provide dose response data on which to base a more accurate reference dose. The study consisted of eight groups of 12 Sprague-Dawley rats, six male and six female, which were exposed to incremental doses …

Bioremediation: Factors Influencing Metal Ion Toxicity In A Toluene Selected Bacterial Population, Scott D. Hansen

Theses and Dissertations

This research was a general phenomenalistic overview of the effects of metals on the respiration rates of a toluene selected microbe culture. The metals studied were copper, zinc, cobalt, manganese and iron. Relative inhibition, Cu>Fe>Zn=Co>Mn, corresponded to hydrogen phosphate binding strengths. Inhibition was found to be pH dependent; it increased with increasing pH and was shown to correlate to an increased adsorption of metal onto the cell. Zn, Cu, and Co toxicities were shown to decrease with increasing magnesium concentrations. This decrease was linked to increased magnesium adsorption and decreased metal adsorption. The magnitude of effect was …

Determining Types Of Health Effects To Persian Gulf Veterans Due To Exposure To Occupational Hazards, Rebecca A. Nelson

Theses and Dissertations

Persian Gulf Veterans' Illnesses (PGVI) consists of multiple illnesses with overlapping symptoms and causes. This research examined health effects literature concerning several occupational chemicals, to identify significant adverse health trends and assess research quality, completeness, and relevance. After compiling this knowledge base, the research focus was narrowed to Chronic Fatigue Syndrome (CFS), solvents, and pyridostigmine bromide (PB). The existing data was analyzed and compared to PGVI health data using the nonparametric statistical method of contingency table analysis to prove or disprove a link between the substance and PGVI. The results of the contingency table analysis were used to make inferences …

Dna Sequences Involved In The Regulation Of Human C-Myc Gene Expression By Herpes Simplex Virus Type 1 (Hsv-1), Shanli Ye

Dissertations and Theses

The human c-myc gene is a cellular proto-oncogene composed of three exons and two introns. Transcription of c-myc is controlled by two promoters, P1 and P2. The activity of these promoters is regulated by many factors, such as cellular transcription factors E2F, YY1, and HSV-1 immediate-early proteins, ICP0, ICP4.

Many regulatory elements located both upstream of and between P1 and P2 have been identified, and some of these are required for optimum expression of c-myc. In this thesis research, a region downstream from P2 in the c-myc exon 1 was identified by its response to transactivation …

Evaluation Of Alternative Crop Rotational Schemes For Cotton Production In Louisiana, Asitava Jana

LSU Historical Dissertations and Theses

Increasing economic importance of cotton production in Louisiana, awareness of the financial and environmental benefits of crop rotations have stressed the need for evaluating cotton production in different rotational schemes. The objective of this study was to estimate profitability of alternative crop rotational schemes within a whole farm context. Yield data for 11 alternative production systems involving cotton, soybean, corn, wheat were obtained from the ongoing crop rotation research at the Northeast Research Station during the period 1983 -1993. Th e systems included continuous, 2 year, and 3 year rotations on two soil types: silt and clay. Price data were …

Movement Patterns And Habitat Use Of The Copperhead Snake (Agkistrodon Contortrix) In Southeastern Virginia, Christopher Edwin Petersen

Biological Sciences Theses & Dissertations

Radiotelemetry was used to study seasonal movement patterns, habitat use, and thermal relations of nine copperhead snakes, Agkistrodon contortrix, in southeastern Virginia from May 1993 to May 1995. Daily movements ranged from 0-450 m, with 0-5 m movements accounting for 65% of the observations. The seasonal movements of males were more extensive than those of females. Differences in seasonal movements between the sexes may be due to reproductive activity of the snakes. Both males and females significantly reduced their movements prior to shedding. More time was spent using upland deciduous forests than lowland river swamps or anthropogenic habitat. Copperheads …

Biochemical And Molecular Characterization Of The Prostate-Specific Membrane Antigen (Psma), John Karl Troyer

Theses and Dissertations in Biomedical Sciences

Prostate cancer is the most common malignancy and the second leading cause of cancer death in males in the United States. Additionally, the number of deaths attributed to prostate cancer is increasing at a rate of approximately 8% a year. Development of new diagnostic and therapy strategies are needed in order to improve the life expectancy of patients with this disease. One tool which may allow for improvements in prostate cancer diagnosis and therapy is the monoclonal antibody (MAb) 7E11-C5.3 which was first described in 1987. Since then, the antigen recognized by MAb 7E11-C5.3 has been named the prostate specific …