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2012

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Molecular Analysis Of The In Situ Growth Rate Of Subsurface Geobacter Species, Dawn E. Holmes, Ludovic Giloteaux, Melissa Barlett, Milind A. Chavan, Jessica A. Smith, Kenneth H. Williams, Michael Wilkins, Philip Long, Derek Lovley Dec 2012

Molecular Analysis Of The In Situ Growth Rate Of Subsurface Geobacter Species, Dawn E. Holmes, Ludovic Giloteaux, Melissa Barlett, Milind A. Chavan, Jessica A. Smith, Kenneth H. Williams, Michael Wilkins, Philip Long, Derek Lovley

Derek Lovley

Molecular tools that can provide an estimate of the in situ growth rate of Geobacter species could improve understanding of dissimilatory metal reduction in a diversity of environments. Whole genome microarray analyses of the subsurface isolate, Geobacter uraniireducens, grown under a variety of conditions identified a number of genes that are differentially expressed at different specific growth rates. Expression of two genes encoding ribosomal proteins, rpsC and rplL, were further evaluated with quantitative reverse transcription PCR (qRT-PCR) in cells with doubling times ranging from 6.56 h to 89.28 h. Transcript abundance of rpsC correlated best (r2= 0.90) with specific growth …


Piscine Myocarditis Virus (Pmcv) In Wild Atlantic Salmon Salmo Salar, Torstein Tengs Dr. Dec 2012

Piscine Myocarditis Virus (Pmcv) In Wild Atlantic Salmon Salmo Salar, Torstein Tengs Dr.

Dr. Torstein Tengs

Cardiomyopathy syndrome (CMS) is a severe cardiac disease of sea-farmed Atlantic salmon Salmo salar L., but CMS-like lesions have also been found in wild Atlantic salmon. In 2010 a double-stranded RNA virus of the Totiviridae family, provisionally named piscine myocarditis virus (PMCV), was described as the causative agent of CMS. In the present paper we report the first detection of PMCV in wild Atlantic salmon. The study is based on screening of 797 wild Atlantic salmon by real-time RT-PCR. The samples were collected from 35 different rivers along the coast of Norway, and all individuals included in the study were …


The Rnf Complex Of Clostridium Ljungdahlii Is A Proton-Translocating Ferredoxin: Nad+ Oxidoreductase Essential For Autotrophic Growth, Pier-Luc Tremblay, Tian Zhang, Shabir A. Dar, Ching Leang, Derek Lovley Dec 2012

The Rnf Complex Of Clostridium Ljungdahlii Is A Proton-Translocating Ferredoxin: Nad+ Oxidoreductase Essential For Autotrophic Growth, Pier-Luc Tremblay, Tian Zhang, Shabir A. Dar, Ching Leang, Derek Lovley

Derek Lovley

It has been predicted that the Rnf complex of Clostridium ljungdahlii is a proton-translocating ferredoxin:NAD+ oxidoreductase which contributes to ATP synthesis by an H+-translocating ATPase under both autotrophic and heterotrophic growth conditions. The recent development of methods for genetic manipulation of C. ljungdahlii made it possible to evaluate the possible role of the Rnf complex in energy conservation. Disruption of the C. ljungdahlii rnf operon inhibited autotrophic growth. ATP synthesis, proton gradient, membrane potential, and proton motive force collapsed in the Rnf-deficient mutant with H2 as the electron source and CO2 as the electron acceptor. Heterotrophic growth was hindered in …


Ict For Poverty Alleviation In Pacific Island Nations: Study Of Icts4d In Fiji, Deogratias Harorimana, Opeti Rokotuinivono, Emali Sewale, Fane Salaiwai, Marica Naulu, Evangelin Roy Dec 2012

Ict For Poverty Alleviation In Pacific Island Nations: Study Of Icts4d In Fiji, Deogratias Harorimana, Opeti Rokotuinivono, Emali Sewale, Fane Salaiwai, Marica Naulu, Evangelin Roy

Dr Deogratias Harorimana

ICT for Poverty Alleviation in Pacific Island Nations: Study of ICTs4D in Fiji There has been a vague and little knowledge on the role or potential of Information and Communications Technologies (ICTs) in relation to addressing poverty in Fiji. This may be probably due to the newness of the technology in the South Pacific Region as a whole but also probably due to the fact that only 9.7% of the current Fiji 931,000 populations are internet users (ITC Figures 2011). This paper reports on finding how ICTs is contributing towards poverty alleviation in Fiji. On the basis of reviewed best …


An Assessment Of The Effects Of Desertification In Yobe State, Nigeria, Jibril Musa Phd Dec 2012

An Assessment Of The Effects Of Desertification In Yobe State, Nigeria, Jibril Musa Phd

Confluence Journal Environmental Studies (CJES), Kogi State University, Nigeria

Desertification is one of the most serious environmental and socio-economic problems of our time. Desertification describes circumstances of land degradation in arid, semi-arid and dry sub-humid regions resulting from the climatic variation and human activities. The fundamental goal of this thesis was to assess the effects of desertification in Yobe State, Nigeria. The present threat of desertification in the sahel has reached an alarming stage where crops cultivation and animal rearing/grazing are no more productive, soil has lost its nutrient/fertility, various infrastructure had given way because of windstorm from the neighbouring Niger Republic and sand dunes had taken over. The …


Buried Clay Pipe Irrigation, David A. Bainbridge Dec 2012

Buried Clay Pipe Irrigation, David A. Bainbridge

David A Bainbridge

The buried clay pipe method of irrigation is one of the most efficient systems known and is ideal for gardeners and small farmers. Buried clay pipe irrigation uses a buried, unglazed clay pipe filled with water to provide controlled irrigation to plants as the water seeps out through the clay wall at a rate that is influenced by the plant's water use. It has been used for more than one hundred years.This auto-regulation leads to very high efficiency--considerably better than drip irrigation and many times better than conventional surface irrigation. This also reduces weeds, increases yields, and can speed maturity …


An Integrated Genetic Linkage Map For Silkworms With Three Parental Combinations And Its Application To The Mapping Of Single Genes And Qtl, Shuai Zhan, Jianhua Huang, Qiuhong Guo, Yunpo Zhao, Weihua Li, Xuexia Miao, Marian R. Goldsmith, Muwang Li, Yongping Huang Dec 2012

An Integrated Genetic Linkage Map For Silkworms With Three Parental Combinations And Its Application To The Mapping Of Single Genes And Qtl, Shuai Zhan, Jianhua Huang, Qiuhong Guo, Yunpo Zhao, Weihua Li, Xuexia Miao, Marian R. Goldsmith, Muwang Li, Yongping Huang

Marian R Goldsmith

Background: Bombyx mori, the domesticated silkworm, is a well-studied model insect with great economic and scientific significance. Although more than 400 mutations have been described in silkworms, most have not been identified, especially those affecting economically-important traits. Simple sequence repeats (SSRs) are effective and economical tools for mapping traits and genetic improvement. The current SSR linkage map is of low density and contains few polymorphisms. The purpose of this work was to develop a dense and informative linkage map that would assist in the preliminary mapping and dissection of quantitative trait loci (QTL) in a variety of silkworm strains. Results: …


Construction And Sequence Sampling Of Deep-Coverage Large-Insect Bac Libraries For Three Model Lepidopteran Species, Chengcang Wu, Dina Proestou, Dorothy Carter, Erica Nicholson, Filippe Santos, Shaying Zhao, Hong-Bin Zhang, Marian R. Goldsmith Dec 2012

Construction And Sequence Sampling Of Deep-Coverage Large-Insect Bac Libraries For Three Model Lepidopteran Species, Chengcang Wu, Dina Proestou, Dorothy Carter, Erica Nicholson, Filippe Santos, Shaying Zhao, Hong-Bin Zhang, Marian R. Goldsmith

Marian R Goldsmith

Background: Manduca sexta, Heliothis virescens, and Heliconius erato represent three widely-used insect model species for genomic and fundamental studies in Lepidoptera. Large-insert BAC libraries of these insects are critical resources for many molecular studies, including physical mapping and genome sequencing, but not available to date. Results: We report the construction and characterization of six large-insert BAC libraries for the three species and sampling sequence analysis of the genomes. The six BAC libraries were constructed with two restriction enzymes, two libraries for each species, and each has an average clone insert size ranging from 152–175 kb. We estimated that the genome …


A Bac-Based Integrated Linkage Map Of The Silkworm Bombyx Mori, Kimiko Yamamoto, Junko Nohata, Keiko Kadono-Okuda, Junko Narukawa, Motoe Sasanuma, Shun-Ichi Sasanuma, Hiroshi Minami, Michihiko Shimomura, Yoshitaka Suetsugu, Yutaka Banno, Kazutoyo Osoegawa, Pieter J. De Jong, Marian R. Goldsmith, Kazuei Mita Dec 2012

A Bac-Based Integrated Linkage Map Of The Silkworm Bombyx Mori, Kimiko Yamamoto, Junko Nohata, Keiko Kadono-Okuda, Junko Narukawa, Motoe Sasanuma, Shun-Ichi Sasanuma, Hiroshi Minami, Michihiko Shimomura, Yoshitaka Suetsugu, Yutaka Banno, Kazutoyo Osoegawa, Pieter J. De Jong, Marian R. Goldsmith, Kazuei Mita

Marian R Goldsmith

Background: In 2004, draft sequences of the model lepidopteran Bombyx mori were reported using whole-genome shotgun sequencing. Because of relatively shallow genome coverage, the silkworm genome remains fragmented, hampering annotation and comparative genome studies. For a more complete genome analysis, we developed extended scaffolds combining physical maps with improved genetic maps. Results: We mapped 1,755 single nucleotide polymorphism (SNP) markers from bacterial artificial chromosome (BAC) end sequences onto 28 linkage groups using a recombining male backcross population, yielding an average inter-SNP distance of 0.81 cM (about 270 kilobases). We constructed 6,221 contigs by fingerprinting clones from three BAC libraries digested …


Extensive Conserved Synteny Of Genes Between The Karyotypes Of Manduca Sexta And Bombyx Mori Revealed By Bac-Fish Mapping, Yuji Yasukochi, Makiko Tanaka-Okuyama, Fukashi Shibata, Atsuo Yoshido, Frantisek Marec, Chengcang Wu, Hongbin Zhang, Marian R. Goldsmith, Ken Sahara Dec 2012

Extensive Conserved Synteny Of Genes Between The Karyotypes Of Manduca Sexta And Bombyx Mori Revealed By Bac-Fish Mapping, Yuji Yasukochi, Makiko Tanaka-Okuyama, Fukashi Shibata, Atsuo Yoshido, Frantisek Marec, Chengcang Wu, Hongbin Zhang, Marian R. Goldsmith, Ken Sahara

Marian R Goldsmith

Background: Genome sequencing projects have been completed for several species representing four highly diverged holometabolous insect orders, Diptera, Hymenoptera, Coleoptera, and Lepidoptera. The striking evolutionary diversity of insects argues a need for efficient methods to apply genome information from such models to genetically uncharacterized species. Constructing conserved synteny maps plays a crucial role in this task. Here, we demonstrate the use of fluorescence in situ hybridization with bacterial artificial chromosome probes as a powerful tool for physical mapping of genes and comparative genome analysis in Lepidoptera, which have numerous and morphologically uniform holokinetic chromosomes. Methodology/Principal Findings: We isolated 214 clones …


Kaikobase: An Integrated Silkworm Genome Database And Data Mining Tool, Michihiko Shimomura, Hiroshi Minami, Yoshitaka Suetsugu, Hajime Ohyanagi, Chikatada Satoh, Baltazar Antonio, Yoshiaki Nagamura, Keiko Kadono-Okuda, Hideyuki Kajiwara, Hideki Sezutsu, Javaregowda Nagaraju, Marian R. Goldsmith, Qingyou Xia, Kimiko Yamamoto, Kazuei Mita Dec 2012

Kaikobase: An Integrated Silkworm Genome Database And Data Mining Tool, Michihiko Shimomura, Hiroshi Minami, Yoshitaka Suetsugu, Hajime Ohyanagi, Chikatada Satoh, Baltazar Antonio, Yoshiaki Nagamura, Keiko Kadono-Okuda, Hideyuki Kajiwara, Hideki Sezutsu, Javaregowda Nagaraju, Marian R. Goldsmith, Qingyou Xia, Kimiko Yamamoto, Kazuei Mita

Marian R Goldsmith

Background: The silkworm, Bombyx mori, is one of the most economically important insects in many developing countries owing to its large-scale cultivation for silk production. With the development of genomic and biotechnological tools, B. mori has also become an important bioreactor for production of various recombinant proteins of biomedical interest. In 2004, two genome sequencing projects for B. mori were reported independently by Chinese and Japanese teams; however, the datasets were insufficient for building long genomic scaffolds which are essential for unambiguous annotation of the genome. Now, both the datasets have been merged and assembled through a joint collaboration between …


16s Ribosomal Dna Sequencing Confirms The Synonymy Of Vibrio Harveyi And V. Carchariae, Eric J. Gauger, Marta Gomez-Chiarri Dec 2012

16s Ribosomal Dna Sequencing Confirms The Synonymy Of Vibrio Harveyi And V. Carchariae, Eric J. Gauger, Marta Gomez-Chiarri

Marta Gomez-Chiarri

Seventeen bacterial strains previously identified as Vibrio harveyi (Baumann et al. 1981) or V. carchariae (Grimes et al. 1984) and the type strains of V. harveyi, V. carchariae and V. campbellii were analyzed by 16S ribosomal DNA (rDNA) sequencing. Four clusters were identified in a phylogenetic analysis performed by comparing a 746 base pair fragment of the 16S rDNA and previously published sequences of other closely related Vibrio species. The type strains of V. harveyi and V. carchariae and about half of the strains identified as V. harveyi or V. carchariae formed a single, wellsupported cluster designed as ‘bona fide’ …


Survival Of Eastern Oysters Crassostrea Virginica From Three Lines Following Experimental Challenge With Bacterial Pathogens, Javier Gomez-Leon, Luisa Villamil, Scott A. Salger, Rachel Sallum, Antonio Remacha-Trivino, Dale F. Leavitt, Marta Gomez-Chiarri Dec 2012

Survival Of Eastern Oysters Crassostrea Virginica From Three Lines Following Experimental Challenge With Bacterial Pathogens, Javier Gomez-Leon, Luisa Villamil, Scott A. Salger, Rachel Sallum, Antonio Remacha-Trivino, Dale F. Leavitt, Marta Gomez-Chiarri

Marta Gomez-Chiarri

Shellfish production is often affected by bacterial pathogens that cause high losses in hatcheries and nurseries. We evaluated the relative survival of larvae and juveniles of 3 Crassostrea virginica oyster lines: (1) GHP, a Rhode Island line; (2) NEHY, a line resistant to dermo and multinucleated sphere X diseases; and (3) FLOWERS, a line resistant to Roseovarius oyster disease, experimental challenge with Vibrio spp. isolates RE22 and RE101, causative agents of bacillary necrosis in Pacific oyster larvae, and the type strain of Roseovarius crassostreae, causative agent of Roseovarius oyster disease. All of the isolates were able to induce significant mortalities …


Infectious Necrotizing Enteritis And Mortality Caused By Vibrio Carachariae In Summer Flounder Paralichthys Dentatus During Intensive Culture, Bruno Soffientino, Todd Gwaltney, David R. Nelson, Jennifer L. Specker, Michael Mauel, Marta Gomez-Chiarri Dec 2012

Infectious Necrotizing Enteritis And Mortality Caused By Vibrio Carachariae In Summer Flounder Paralichthys Dentatus During Intensive Culture, Bruno Soffientino, Todd Gwaltney, David R. Nelson, Jennifer L. Specker, Michael Mauel, Marta Gomez-Chiarri

Marta Gomez-Chiarri

An epizootic causing mortality among cultured summer flounder Paralichthys dentatus occurred in summer of 1998 at a land-based facility on Narragansett Bay, Rhode Island, USA. The disease, flounder infectious necrotizing enteritis (FINE), was characterized by reddening around the anal area, distended abdomens filled with opaque serosanguineous fluid, enteritis and necrosis of the posterior intestine. In extreme cases of the disease, the posterior intestine was detached from the anus and was observed coming out the vent. The intestine of individuals that recovered from the dsease ended in a blind-sac; the abdomens of these fish were distended, due to food and water …


Using Remote Sensing Data To Improve Rice Production In Kutigi, Niger State, Nigeria, Jibril Musa Phd, M B. Yunusa Dec 2012

Using Remote Sensing Data To Improve Rice Production In Kutigi, Niger State, Nigeria, Jibril Musa Phd, M B. Yunusa

Confluence Journal Environmental Studies (CJES), Kogi State University, Nigeria

This research work looked in the used of Remote Sensing to improve Agricultural production in Kutigi, Niger State. The aim of the study is to use remote sensing to improve rice farming activities in Kutigi, Niger State. It is very important to identify such methods to improve Agricultural production because experts are always interested in new researches and findings to better the standard of living in any environment. In view of this, Remotely-sensed data could be used or employed to elevate most of these agricultural problems in Kutigi through the following objectives: Using Landsat imagery to assess the present landuse …


Effect Of Potassium Fertilizer And Different Fertilization Methods On The Growth Of Tuberose, A Mohammadi Torkashvand, Yaser Shariati, R Onsinejad Dec 2012

Effect Of Potassium Fertilizer And Different Fertilization Methods On The Growth Of Tuberose, A Mohammadi Torkashvand, Yaser Shariati, R Onsinejad

y shariati

An appropriate fertilization method is an important topic in nutrition of ornamental plants for obtaining suitable quality and quantity yield. This study was conducted in Farvardin greenhouse of Mashhad Township, Khorasan province, Iran, in summer and autumn 2011 to find appropriate fertilization method on the growth of tuberose as the test plant. The experiment was performed by 21 treatments and 3 replications in a randomized completely design. Different methods of fertilization by different fertilizers in three levels of basic potassium were evaluated. The treatment containing 100 kg potassium as base + one-third N and P as base and two-third N …


The Action Component Of Recognition Systems: A Focus On The Response, Aviva E. Liebert, Philip T. Starks Dec 2012

The Action Component Of Recognition Systems: A Focus On The Response, Aviva E. Liebert, Philip T. Starks

Aviva E Liebert

The action component of recognition systems is concerned with the response of an evaluator to perceived cues, based on the dissimilarity of those cues compared with a template. Building upon the historical focus on kin recognition, we apply the framework of conspecific acceptance thresholds to recognition in a broader sense, including interactions within and between organisms, between species, and between living and nonliving things. We review examples from a variety of taxa and recognition contexts to demonstrate when a discriminatory response is expected, and how this response may be flexible depending on the costs and benefits of recognition errors and …


Evolution Of Repetitive Proteins: Spider Silks From Nephila Clavipes (Tetragnathidae) And Araneus Bicentenarius (Araneidae), Richard D. Beckwitt, Steven Arcidiacono, Robert Stote Dec 2012

Evolution Of Repetitive Proteins: Spider Silks From Nephila Clavipes (Tetragnathidae) And Araneus Bicentenarius (Araneidae), Richard D. Beckwitt, Steven Arcidiacono, Robert Stote

Richard D Beckwitt

Spider silks are highly repetitive proteins, characterized by regions of polyalanine and glycine-rich repeating units. We have obtained two variants of the Spidroin 1 (NCF-1) silk gene sequence from Nephila clavipes. One sequence (1726 bp) was from a cloned cDNA, and the other (1951 bp) was from PCR of genomic DNA. When these sequences are compared with each other and the previously published Spidroin 1 sequence, there are differences due to sequence rearrangements, as well as single base substitutions. These variations are similar to those that have been reported from other highly repetitive genes, and probably represent the results …


Sequence Conservation In The C-Terminal Region Of Spider Silk Proteins (Spidroin) From Nephila Clavipes (Tetragnathidae) And Araneus Bicentenarius (Araneidae), Richard D. Beckwitt, Steven Arcidiacono Dec 2012

Sequence Conservation In The C-Terminal Region Of Spider Silk Proteins (Spidroin) From Nephila Clavipes (Tetragnathidae) And Araneus Bicentenarius (Araneidae), Richard D. Beckwitt, Steven Arcidiacono

Richard D Beckwitt

The Polymerase Chain Reaction (PCR) has been used to amplify the portion of the Spidroin 1 gene that codes for the C-terminal part of the silk protein of the spider Nephila clavipes. Along with some substitution mutations of minor consequence, the PCR-derived sequence reveals an additional base missing from the previously published Nephila Spidroin 1 sequence. Comparison of the PCR-derived sequence with the equivalent region of Spidroin 2 indicates that the insertion of this single base results in greatly increased similarity in the resulting amino acid sequences of Spidroin 1 and Spidroin 2 (75% over 97 amino acids). The same …


Predicting Avian Distributions To Evaluate Spatiotemporal Overlap With Locust Control Operations In Eastern Australia, Judit K. Szabo, Pamela J. Davy, Michael Hooper, Lee Astheimer Dec 2012

Predicting Avian Distributions To Evaluate Spatiotemporal Overlap With Locust Control Operations In Eastern Australia, Judit K. Szabo, Pamela J. Davy, Michael Hooper, Lee Astheimer

Dr Pamela Davy

Locusts and grasshoppers cause considerable economic damage to agriculture worldwide. The Australian Plague Locust Commission uses multiple pesticides to control locusts in eastern Australia. Avian exposure to agricultural pesticides is of conservation concern, especially in the case of rare and threatened species. The aim of this study was to evaluate the probability of pesticide exposure of native avian species during operational locust control based on knowledge of species occurrence in areas and times of application. Using presence-absence data provided by the Birds Australia Atlas for 1998 to 2002, we developed a series of generalized linear models to predict avian occurrences …


أمراض الأطفال المزمنة, Suad Fahad Alferaih Dec 2012

أمراض الأطفال المزمنة, Suad Fahad Alferaih

Dr. Suad Fahad AlFuraih

No abstract provided.


Genetic Determinants Of Cerebral Edema In Severe Traumatic Brain Injury: A Pilot Study Of The Role Of Cacna1 And Aqp4 Gene Mutations, Raphael A. Carandang, Susanne Muehlschlegel, Wiley R. Hall, Cynthia Ouillette, Robert H. Brown Jr. Dec 2012

Genetic Determinants Of Cerebral Edema In Severe Traumatic Brain Injury: A Pilot Study Of The Role Of Cacna1 And Aqp4 Gene Mutations, Raphael A. Carandang, Susanne Muehlschlegel, Wiley R. Hall, Cynthia Ouillette, Robert H. Brown Jr.

Dr Robert Brown

Cerebral edema is the one of the most significant predictors of poor outcome after traumatic brain injury. It is still unclear what the pathophysiological and cellular mechanisms and predictors of post-traumatic edema are. The exponential growth in genetic information has opened an avenue for investigation in traumatic brain injury and implicated specific genes in the pathophysiology of post-traumatic injury edema. Two examples are the Aquaporin-4 and CACNA1 genes, which respectively encode water and calcium channels. The Aquaporin-4 gene on chromosome 18q11.2-12.1 encodes the Aquaporin-4 protein (AQP4) water channel. AQP4 is one of the bidirectional high capacity water channels that is …


Homologous Recombination Mediates Functional Recovery Of Dysferlin Deficiency Following Aav5 Gene Transfer, William E. Grose, K. Reed Clark, Danielle Griffin, Vinod Malik, Kimberly M. Shontz, Chrystal L. Montgomery, Sarah Lewis, Robert H. Brown Jr., Paul M. L. Janssen, Jerry R. Mendell, Louise R. Rodino-Klapac Dec 2012

Homologous Recombination Mediates Functional Recovery Of Dysferlin Deficiency Following Aav5 Gene Transfer, William E. Grose, K. Reed Clark, Danielle Griffin, Vinod Malik, Kimberly M. Shontz, Chrystal L. Montgomery, Sarah Lewis, Robert H. Brown Jr., Paul M. L. Janssen, Jerry R. Mendell, Louise R. Rodino-Klapac

Dr Robert Brown

The dysferlinopathies comprise a group of untreatable muscle disorders including limb girdle muscular dystrophy type 2B, Miyoshi myopathy, distal anterior compartment syndrome, and rigid spine syndrome. As with other forms of muscular dystrophy, adeno-associated virus (AAV) gene transfer is a particularly auspicious treatment strategy, however the size of the DYSF cDNA (6.5 kb) negates packaging into traditional AAV serotypes known to express well in muscle (i.e. rAAV1, 2, 6, 8, 9). Potential advantages of a full cDNA versus a mini-gene include: maintaining structural-functional protein domains, evading protein misfolding, and avoiding novel epitopes that could be immunogenic. AAV5 has demonstrated unique …


Differentially Expressed Genes In Populus Simonii×Populus Nigra In Response To Nacl Stress Using Cdna-Aflp, Lei Wang, Boru Zhou, Lili Wu, Baozhu Guo, Tingbo Jiang Dec 2012

Differentially Expressed Genes In Populus Simonii×Populus Nigra In Response To Nacl Stress Using Cdna-Aflp, Lei Wang, Boru Zhou, Lili Wu, Baozhu Guo, Tingbo Jiang

Dr Lei Wang

Salinity is an important environmental factor limiting growth and productivity of plants, and affects almost every aspect of the plant physiology and biochemistry. The objective of this study was to apply cDNA-AFLP and to identify differentially expressed genes in response to NaCl stress vs. no-stress in Populus simonii × Populus nigra in order to develop genetic resources for genetic improvement. Selective amplification with 64 primer combinations allowed the visualization of 4407 transcript-derived fragments (TDFs), and 2027 were differentially expressed. Overall, 107 TDFs were re-sequenced successfully, and 86 unique sequences were identified in 10 functional categories based on their putative functions. …


The Genome Of Pelobacter Carbinolicus Reveals Surprising Metabolic Capabilities And Physiological Features, Muktak Aklujkar, Shelley A. Haveman, Raymond Didonato, Olga Chertkov, Cliff S. Han, Miriam L. Land, Peter Brown, Derek Lovley Dec 2012

The Genome Of Pelobacter Carbinolicus Reveals Surprising Metabolic Capabilities And Physiological Features, Muktak Aklujkar, Shelley A. Haveman, Raymond Didonato, Olga Chertkov, Cliff S. Han, Miriam L. Land, Peter Brown, Derek Lovley

Derek Lovley

BackgroundThe bacterium Pelobacter carbinolicus is able to grow by fermentation, syntrophic hydrogen/formate transfer, or electron transfer to sulfur from short-chain alcohols, hydrogen or formate; it does not oxidize acetate and is not known to ferment any sugars or grow autotrophically. The genome of P. carbinolicus was sequenced in order to understand its metabolic capabilities and physiological features in comparison with its relatives, acetate-oxidizing Geobacter species.ResultsPathways were predicted for catabolism of known substrates: 2,3-butanediol, acetoin, glycerol, 1,2-ethanediol, ethanolamine, choline and ethanol. Multiple isozymes of 2,3-butanediol dehydrogenase, ATP synthase and [FeFe]-hydrogenase were differentiated and assigned roles according to their structural properties and …


Becoming Differently Modern: Geographic Contributions To A Generative Climate Politics, Lesley M. Head, Christopher R. Gibson Dec 2012

Becoming Differently Modern: Geographic Contributions To A Generative Climate Politics, Lesley M. Head, Christopher R. Gibson

Chris Gibson

Anthropogenic climate change is a quintessentially modern problem in its historical origins and discursive framing, but how well does modernist thinking provide us with the tools to solve the problems it created? On one hand even though anthropogenic climate change is argued to be a problem of human origins, solutions to which will require human actions and engagements, modernity separates people from climate change in a number of ways. On the other, while amodern or more-than-human concepts of multiple and relational agency are more consistent with the empirical evidence of humans being deeply embedded in earth surface processes, these approaches …


Engaging Creative Communities In An Industrial City Setting, Chris Gibson, Ben Gallan, Andrew Warren Dec 2012

Engaging Creative Communities In An Industrial City Setting, Chris Gibson, Ben Gallan, Andrew Warren

Chris Gibson

Much has been said about how ‘creativity’ might infuse policymaking and planning – especially in the wake of popular bestsellers by Richard Florida and Charles Landry on ‘creative places’ and the ‘creative class’ (the latter a supposed demographic group associated with creative industries such as film, design and music, who are said to be the key to the economic fortunes of cities). Creativity, it is said, can be facilitated in particular urban environments, given the right preconditions such as ‘hip’ inner-city precincts, café culture and walkable dense clusters of design firms and retail and residential spaces. The common argument is …


Living Together But Apart: Material Geographies Of Everyday Sustainability In Extended Family Households, Natascha Klocker, Chris Gibson, Erin Borger Dec 2012

Living Together But Apart: Material Geographies Of Everyday Sustainability In Extended Family Households, Natascha Klocker, Chris Gibson, Erin Borger

Chris Gibson

In the Industrialized West, ageing populations and cultural diversity-combined with rising property prices and extensive years spent in education-have been recognized as diverse factors driving increases in extended family living. At the same time, there is growing awareness that household size is inversely related to per capita resource consumption patterns, and that urgent problems of environmental sustainability are negotiated, on a day-to-day basis (and often unconsciously), at the household level. This paper explores the sustainability implications of everyday decisions to fashion, consume, and share resources around the home, through the lens of extended family households. Through interviews with extended family …


Trial By Fire: Natural Hazards, Mixed-Methods And Cultural Research, Christine Eriksen, Nicholas J. Gill, Ross A. Bradstock Dec 2012

Trial By Fire: Natural Hazards, Mixed-Methods And Cultural Research, Christine Eriksen, Nicholas J. Gill, Ross A. Bradstock

Christine Eriksen

This paper considers the issues of research 'relevance' and 'use' to reflect upon a cultural geography research project on bushfire that did not begin with any specific aim of being useful to policy makers but which has garnered considerable and ongoing interest from a broad audience. It provides an example of how the integration of quantitative and qualitative research methods and data can enhance research into cultural aspects of natural hazards whilst simultaneously playing a key role in ensuring that the research results are of interest to a wide range of groups. Using a mixed-methods research approach was found to …


The Art Of Learning: Wildfire, Amenity Migration And Local Environmental Knowledge, Christine Eriksen, T Prior Dec 2012

The Art Of Learning: Wildfire, Amenity Migration And Local Environmental Knowledge, Christine Eriksen, T Prior

Christine Eriksen

Communicating the need to prepare well in advance of the wildfire season is a strategic priority for wildfire management agencies worldwide. However, there is considerable evidence to suggest that although these agencies invest significant effort towards this objective in the lead up to each wildfire season, landholders in at-risk locations often remain under-prepared. One reason for the poor translation of risk information materials into actual preparation may be attributed to the diversity of people now inhabiting wildfire-prone locations in peri-urban landscapes. These people hold widely varying experiences, beliefs, attitudes and values relating to wildfire, which influence their understanding and interpretation …