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Propagation And In Vitro Studies Of Perkinsus Marinus, Jerome F. Lapeyre

VIMS Articles

The development of continuous cultures of Perkinsus marinus (Apicomplexa) is a major breakthrough that will lead to a better understanding of this deadly oyster pathogen. More than 10 P. marinus isolates are currently in continuous cultures. Culture media used to propagate P. marinus range from media designed for the culture of mammalian cells to protein-free chemically defined media. Continuous cultures of P. marinus can be initiated from a variety of infected oyster tissues or from isolated hypnospores (i.e., the enlarged stage of P. marinus from oyster tissue incubated in Ray's fluid thioglycollate medium). P. marinus cells adapt well to culture …