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Using Rna Sequencing Methodologies To Uncover Common Pathways In Neural Tube Defects: A Comparative Study Of Three Oxidative Neurotoxicants, Aubrey Coleen Johansen

Theses and Dissertations

Neural tube defects (NTDs) are the second most common type of congenital birth defect that affect infants worldwide. There are several proposed mechanisms for NTDs, but no definitive mechanism has yet been described. One possible mechanism is oxidative disruption of normal developmental signaling. The purpose of this study is to culture whole mouse embryos with three common developmental toxicants, mono-2-ethylhexyl phthalate (MEHP, a plastic pollutant), Fumonisin B1 (FB1, a corn mold), and valproic acid (VPA, an anticonvulsant drug), all of which are known to cause NTDs, and compare their shifts in redox potential and changes to important signaling pathways through …

Investigating The Antibacterial And Immunomodulatory Effects Of Resolvin D2 On Monocytes And Macrophages, Cristina Maria Padovani

Theses and Dissertations

In the late phase of sepsis, immunosuppression occurs, where the host is unable to clear the pre-existing infection and is susceptible to secondary infections. It is believed that the ideal treatments for sepsis should attenuate immunosuppression so that the host can get back to homeostasis. Specialized Pro-resolving Mediators (SPMs) are endogenously- produced fatty acids that resolve infectious inflammation without being immunosuppressive. We hypothesize that an SPM – Resolvin D2 (RvD2) – can augment exhausted macrophage function during the immunosuppressive phase of sepsis. We developed a two-hit model to establish macrophage exhaustion in vitro, and found that RvD2 increased NF-κB activity, …

Forest Schools, Ecofeminism, The Gender Binary, And Androcentrism, Jana Elizabeth Schwai

Theses and Dissertations

Gender in forest schools is a topic that should be at the forefront of discussion when creating a forest school, its pedagogy, curriculum, and principles. Gender is a large part of who we are as humans and having teachers aware of its complexities, presentation, and presence in the forest school setting is imperative. This study consists of interviews and focus group data collected at a midwest United States public forest preschool and an eastern United States private forest preschool. The teachers at these schools were cisgender, as were the students ages three through five who were observed. This paper analyzes …

Walk Or Swim: The Substrate For Movement Affects Mate Choice Behaviors In Females Gray Treefrogs (Hyla Versicolor), Hannah Warner

Theses and Dissertations

Semiaquatic animals breed in environments with a mix of aquatic and terrestrial features, each requiring appropriate types of locomotion with differential energetic demands and containing different suits of predators. We surveyed calling locations of male Eastern Gray Treefrogs (Hyla versicolor) at our study pond to assess average composition of land/water substrates available to females during mate choice. We also conducted acoustic playback trials to examine whether the mate choice behavior of females was affected by approach substrate; i.e., whether females had to swim or walk towards a potential mate. We found that mate preferences were not strongly affected by the …

Investigating An Optimal Motor Learning Protocol That Leads To Better Savings And Long-Term Retention Of Visuomotor Adaptation, Reshma James

Theses and Dissertations

Visuomotor adaptation is fundamental to everyday activities, from the simple act of reaching for a cup to complex athletic maneuvers. The concept of savings is particularly interesting as it sheds light on how motor skills are acquired, retained, and relearned over time. Savings refers to the phenomenon where motor skills that have been previously learned are reacquired or relearned more rapidly after a period of inactivity or after the individual has undergone de-adaptation. While it was initially believed that savings in motor adaptation stemmed from implicit learning mechanisms, recent research indicates that it is primarily attributed to explicit recall of …

Which Vole Is Which: Dna-Based Species Identification For Wisconsin’S Three Microtus Species, Madeline Noel Opie

Theses and Dissertations

Accurate species identification is necessary to implement conservation strategies in the wild. When traditional morphology-based species identification is challenging due to phenotypic plasticity, overlapping characteristics, or the species are otherwise cryptic, DNA-based species identification may be more suitable. Of the three species of Microtus in Wisconsin, two are listed as threatened at the state level. Both M. ochrogaster and M. pinetorum have stable population levels at the national level but are along the northern edge of their ranges in Wisconsin. Small and vulnerable populations of M. ochrogaster and M. pinetorum are limited to isolated patches in the southwestern portion of …

Quantifying Nutrients At Wisconsin Freshwater Beaches And Determining The Molecular Response Of E. Coli To Nutrient Limitation, Sophia Ward

Theses and Dissertations

Escherichia coli (E. coli) are popular fecal indicator bacteria (FIB) used by many beach management programs in Wisconsin to evaluate the health risks posed by beaches. Prolonged survival of E. coli in beach sand undermines beach management practices that rely on FIB enumeration in surface water because sand and water are routinely mixed through wave action. Studies have shown that sand is a favorable environment for E. coli due to temperature, moisture, nutrient content, and protection from UV radiation. In addition, some sources of E. coli pose significantly less of a threat to human health than human fecal waste, but …

Accuracy And Precision Of Wearable Camera Media Annotations To Estimate Dimensions Of Physical Activity And Sedentary Behavior, Julian Martinez

Theses and Dissertations

Physical activity (PA) is a complex behavior to measure given its sporadic nature and its all-encompassing definition as bodily movement produced by skeletal muscles that result in increased energy expenditure. Sedentary behavior (SB) is another complex health behavior to measure as it is more exactly defined as sitting, reclining, or lying while having an energy expenditure ≤ 1.5 metabolic equivalents (METs). For both PA and SB, it is important to measure the dimensions of frequency, intensity, duration and type of activity being performed when associating the volume of PA and SB with different health outcomes. However, commonly used measurement tools …

The Complexity Of Female Treefrog Mate Choice: Choosiness And Preferences Are Independent Behaviors And Each Are Prone To Separate Sources Of Variation, Olivia S. Feagles

Theses and Dissertations

Mate choice is an important cause of natural and sexual selection, driving the evolution of ornaments and promoting diversification and speciation. Mate choice decisions arise from the interaction of several components, and knowledge of whether they interact, and how, is crucial for understanding their contributions to selection. There are multiple components of mate choice theoretically proposed, and here I focus specifically on preference functions (attractiveness ranking of prospective mates) and/or choosiness (willingness to invest effort in obtaining the preferred mate). Objective 1 (addressed in Chapter I) of this dissertation focuses specifically on the relationship between preferences and choosiness, testing the …

Quantifying Within-Population Variation In Mate Choice; Discrimination, Mate Sampling Rules, Multivariate Preference & Consequences For Sexual Selection, Kane David Stratman

Theses and Dissertations

Mate choice is foundational to the evolution of elaborate, conspicuous, and often energetically costly displays. Modelling sexual selection in any mating system is a complicated task on two fronts; we typically confront variation in both i) the strategies of signalers and ii) how choosers assess and sample among them. As selection on mate choice behaviors emerges from the relationship between these sources of variation, it is essential that they be measured in detail. Population-level measures of chooser behaviors (i.e. pooling the single responses of a sample of choosers) have long dominated the literature on mate choice, leaving open the possibility …

Implementing Fret Spectrometry Using Time Resolved Fluorescence Microscopy For Determination Of Protein Oligomer Size And Geometry In Live Cells, Aliyah Sephrah Khan

Theses and Dissertations

Fӧrster or Fluorescence Resonance Energy Transfer (FRET) is a biological phenomenon that occurs when energy is transferred non-radiatively from an excited donor molecule to an unexcited acceptor molecule when they are a certain distance from each other. One method of conducting FRET experiments is using FRET spectrometry which was previously introduced by the Raicu Lab. This method generates histograms of FRET efficiencies at pixel level called FRET spectrograms, that are fitted with models to determine the quaternary structure of protein oligomers as opposed to traditional FRET experiments which average over all FRET efficiencies. Currently, FRET spectrometry is implemented with spectrally …

Dexterous Manipulation Capabilities Are Associated With Change In Discharge Rate Properties Of Motor Neurons With Age, Mukta N. Joshi

Theses and Dissertations

Aging is accompanied by declines in manual dexterity and fine motor control. The purpose of this research was to compare hand motor control in young and older adults and examine the neuromuscular mechanisms responsible for enabling these interactions. We test force variability during isometric and dynamic contractions, manual dexterity and track motor unit activity to identify the neuromuscular mechanisms responsible for changes in dexterity with age. 26 older adults (66-86 years) and 28 young adults (19 – 38 years) participated in the study. Research participants performed force matching tasks during index finger abduction, precision pinch, static pressing and hybrid force/ …

Physical Activity, Cardiorespiratory Fitness And All-Cause Mortality, Jorin Dane Larsen

Theses and Dissertations

Introduction Both elevated levels of physical activity (PA) and high cardiorespiratory fitness (CRF) are associated with a decreased risk of all-cause mortality. The degree to which an individual's PA level and CRF status interact to influence mortality is not currently fully understood. This study investigated whether PA and CRF were independently related to all-cause mortality, and to what degree PA was associated with improved mortality risk in individuals with low CRF. Methods This is a prospective cohort study using health assessments on individuals gathered between 1975 and 2002. Health assessment data were matched with the National Death Registry to assess …

Conservation And Management Of Greater Sage-Grouse In Strawberry Valley: Quantifying Influences On A Traditional Capture Method And Long-Term Trends In Clutch Size, Janae Radke

Theses and Dissertations

The greater sage-grouse (Centrocercus urophasianus) is a species of conservation concern that has undergone serious declines in the last century. The Strawberry Valley sage grouse population in Utah underwent such a decline from an estimated 3,500 sage grouse in the 1930s to 150 sage grouse in the early 2000s. This decline initiated a long-term conservation and monitoring project in Strawberry Valley with the goal of preserving the sage grouse population. As part of this ongoing conservation project, we investigated factors that impact the success of capturing sage grouse using the night-lighting method (Chapter 1). We found that capture success is …

The Role Of Caloric Intake On Achilles Tendon Health In Pre-Professional Ballet Dancers, Annie G. Smedley

Theses and Dissertations

Background: Achilles tendinopathy is a common and debilitating condition among female ballet dancers due to the large repetitive loading forces placed on their Achilles tendons during rehearsals and performances. Tendon health problems in females are exacerbated by a lack of understanding about how energy availability influences tendons. Ballet dancers, as aesthetic athletes, are vulnerable to low energy availability and can enter a spectrum disorder, relative energy deficiency in sport, that consists of low energy availability (with or without disordered eating), menstrual cycle dysfunction, and low bone mineral density (BMD). Aims: 1) To investigate the relationship between insufficient caloric intake and …

Exploring Mesolimbic Circuitry Modulation By Opiates, Interleukin-10, And Psychostimulants, Joakim W. Ronström

Theses and Dissertations

The mesolimbic dopamine (DA) system originates in the ventral tegmental area (VTA) and projects to the nucleus accumbens (NAc) and other areas including the basolateral amygdala (BLA), prefrontal cortex, and the hippocampus. Drug use induces reward and leads to dysregulation in these brain areas and eventually to substance use disorders (SUDs). Chapter 1 introduces the mesolimbic DA system and its relationship to drug use and their relevance to each chapter. Chapter 2 explores opioid effects on BLA circuitry which is known to play a role in the emotional response including anxiety and stress in SUDs. We showed that morphine induced …

Characterization Of Cellular Metabolism Regulation By The Transcription Factor Centromere Binding Factor 1 (Cbf1), Spencer Ellsworth

Theses and Dissertations

Centromere binding factor 1 (Cbf1) is a transcription factor that controls the transcription of many genes involved in cellular respiration and lipid biogenesis and, as such, has been associated with hypolipidemia in humans. It is a known substrate for PAS kinase, which phosphorylates Cbf1 and alters its activity. Our hypothesis is that this phosphorylation affects the genes it regulates and the DNA motifs it binds to, perhaps due to different transcription complexes being formed. In this study, we conduct a chromatin immunoprecipitation in Saccharomyces cerevisiae to determine what genes and DNA motifs Cbf1 binds to in its wild type versus …

The Effects Of Diesel Exhaust Particle Exposure On Adipos Mitochondrial Bioenergetics And Inflammation, Cali Elizabeth Warren

Theses and Dissertations

Fine particulate matter (PM2.5) constitutes a significant component of ambient air pollution that has been implicated in the pathogenesis of metabolic disorders, including insulin resistance and type 2 diabetes. Among PM2.5 constituents, diesel exhaust particles (DEP) are prevalent particulates that infiltrate the bloodstream to drive systemic pathologies. The purpose of this study was to characterize the metabolic response of adipose tissue to DEP. We aimed to provide a comprehensive understanding by exploring mitochondrial bioenergetics, characterizing the inflammatory marker profile, including adipokines, and conducting a detailed histological analysis of adipocytes to provide valuable insights to the evolving understanding of the intricate …

Applicability Of Using Bio-Receptive Concrete For Building Facades In Egypt, Gina Roupheil

Theses and Dissertations

The significant increase in carbon dioxide emissions caused by the construction industry is detrimental to our planet. This is rapidly increasing with the urbanization of cities that is gradually taking its toll on the available green spaces, which help in balancing such emissions. Recently, researchers have been trying to make use of bio-receptivity to create biomaterial systems that could be spread on building envelopes and support the growth of small plant species and microorganisms to establish on. Out of those materials, comes the concrete as a promising material for bio-colonization.

Within this context, this study aims at investigating the applicability …

Integrative Genomic Insights Into Coral Resilience: Adaptive And Acclimatory Responses To Seasonal Environmental Shifts, Tasnim Ghanim

Theses and Dissertations

Coral reefs, critical to marine biodiversity and coastal protection, face escalating threats from climate change-related phenomena such as ocean warming. This warming is leading to widespread thermal stress that contributes to coral bleaching and infectious disease in corals, leading to the disruption of marine ecosystems and the economies that depend on them. Focusing on the brain coral Platygyra daedalea, known for its thermal resilience in the Persian Arabian Gulf—a region that exemplifies the extreme stressors of climate change—our study aims to dissect the genetic, transcriptomic, and microbiological underpinnings of coral survival in high-temperature environments predicted for the end of …

Impairment Of The Glial Phagolysosomal System Drives Prion-Like Propagation Of Huntington’S Disease, Graham Davis

Theses and Dissertations

The ability of glia to tightly regulate neuronal health and homeostasis in the CNS is conserved across species. Yet, despite the ability to degrade protein aggregates, glia are vulnerable to the accumulation of neurotoxic amyloid aggregates during neurodegenerative disease progress, and even exacerbate their spread. A developing narrative highlights glia as a double-edged sword in neurodegenerative diseases: initially capable of dynamically responding to amyloid aggregate-ladened dying neurons but also capable of inducing chronic inflammation and creating seeding-competent amyloid oligomers. Thus, uncovering the mechanisms that allow glia to control aggregate deposition while preventing the neurotoxic effects and seed generation is vital …

Stacking And Intersecting Nutrient 4rs On Potato, Samuel Hamilton Stapley

Theses and Dissertations

The 4Rs of nutrient management is a global agronomic initiative with an aim to improve the sustainability of major cropping systems and the environment. The objective for this project is to evaluate individual and stacked 4R management practices and how they intersect. A field trial near Grace, Idaho was conducted on potato (Solanum tuberosum L.) in 2020 and 2023. Nitrogen (N) fertilizer treatments included all combinations of two sources [urea or polymer coated urea (PCU)], two rates (82 or 100% of the recommended rate), and/or two timing/placements (all applied at emergence or 84% at emergence + 16% fertigation simulation) compared …

Exploring A Gene Panel For Parkinson’S Disease In An Egyptian Cohort, Asmaa Saeed Gabr

Theses and Dissertations

Parkinson’s disease is a highly heterogeneous disorder characterized by diverse neuropathological features, clinical presentations and progression patterns. In Egypt, Parkinson’s disease incidence rates lie outside the range reported elsewhere. The genetic background to the pathogenesis of Parkinson's disease has been postulated for a long time. However, Parkinson’s disease has never been systematically investigated in Egypt. This study aimed to explore genetic variants and interactions that are associated with the familial and sporadic forms of Parkinson's disease in an Egyptian cohort. This includes examining variants in PD-related genes, exploring the role of specific genes like MAPT and adjacent genomic regions, and …

The Relationship Between Cognitive Impairment In Psychiatric Patients And Readmission Rate To An Inpatient Facility, Cherilyn Isis Schuff

Theses and Dissertations

The primary intention of this study was to further understand the impact of assessing cognitive impairment in psychiatric patients, as a mediating factor on readmission rates. Mild cognitive dysfunction impacts a patient’s functional outcomes (Bowie & Harvey, 2006; Davis et al., 2012; Marcantonio, et al., 2001). Little information exists to guide best practices in the treatment of adults with cognitive impairment who are hospitalized for acute conditions (Davis et al., 2012). A cognitive impairment may impact patient prognosis and ability to function outside of a setting focused on stabilization. Neuropsychological testing is a valuable tool in predicting a patient’s cognitive …

The Role Of The Cdk8 Kinase Module In Maintaining Proteostasis, Stephen Willis

Theses and Dissertations

The underlying etiology of numerous disease states results from perturbations in the maintenance of cellular proteostasis. Carcinogenesis relies on these perturbations to foster uncontrolled cell growth and eventual metastases, while neurodegenerative diseases are a consequence of such perturbations. Control of these processes occurs at numerous molecular levels, commonly starting with transcription. A key transcriptional complex that is involved is the CDK8 Kinase Module (CKM). The CKM is conserved from yeast to man, forming a tetrameric complex consisting of MED12, MED13, CDK8, and CCNC. The CKM has not only been implicated in a variety of cancers but also in a spectrum …

Elastin-Like Polypeptide As A Model To Study Intrinsically Disordered Proteins, Sadegh Majdi

Theses and Dissertations

The elastin-like polypeptide (ELP) is a well-studied structural protein that is easily amenable to amino acid (AA) sequence modifications and has the potential to yield a wide variety of uses in bioengineering and biomedical applications. One unique property of ELP is the inclusion of intrinsically disordered domains (IDP) within the structure that allow for its diversity of physical properties. While it is generally understood that amino acid sequence dictates protein folding arrangements, the contributions of specific amino acid sequences to the intrinsic disorder of ELP has yet to be fully resolved. Therefore, identifying the contributions of specific amino acid sequences …

Persistence Of Wastewater-Associated Antibiotic Resistant Bacteria In River Microcosms, Aoife P. Mahaney

Theses and Dissertations

The spread of antibiotic-resistant bacteria (ARB) associated with wastewater is a significant environmental concern, but little is known about the persistence and proliferation of these organisms in receiving water bodies after discharge. To address this knowledge gap, we performed a series of microcosm experiments in which river water was amended with either untreated or treated wastewater, and the abundance of viable ciprofloxacin-, Bactrim-, and erythromycin-resistant bacteria was monitored for 72 hours.

Both types of wastewater amendments increased the initial abundance of ARB compared to microcosms containing only river water. The increase was greatest with untreated wastewater, but that effect decreased …

Mathematical Modeling And Analysis Of Inflammation And Tissue Repair: Lung Inflammation And Wound Healing In Corals Under Stress, Quintessa Hay

Theses and Dissertations

A variety of insults, including tissue injury and/or exposure to pathogen, elicit an immune response in many organisms. An improperly regulated immune response can result in deleterious effects to the organism. Here we present models for lung injury in young and old mice and models for wound healing in coral reefs.

It is well known that the immune response becomes less effective in older individuals. This is of particular interest in pulmonary insults such as ventilator induced lung injury (VILI) or lung infection. We extended a mathematical model for the inflammatory response to VILI and used experimental data to select …

Investigating Ammonium Toxicity In The Performance Of Recirculating Periphytic Algal Wastewater Treatment Systems, Oluchuku Richie Ochonogor

Theses and Dissertations

Freshwater nutrient enrichment from wastewater facilities and other sources can lead to freshwater eutrophication, a threat to global aquatic ecosystems. Mechanical and chemical ways to curb this threat are either too expensive or not sustainable, and thus, not feasible. Compared to mechanical and other methods, sustainable, inexpensive biological methods (for example, algal treatment systems) have therefore been developed for the removal of excess nutrients from wastewater. By design, secondary waste treatment facilities (WWTF) remove organics and solids and lower oxygen demanding substances; however, they may not remove enough nutrients to protect freshwater ecosystems in all cases. While algae-based biological methods …

An Exploratory Study Of The Effects Of Bis-Substituted Triazolium Bromide Salts On The Proliferation Of Wi-38 Lung Cells And H-1299 Lung Cancer Cells, Rakaiya Martin

Theses and Dissertations

Lung cancer is the leading cause of cancer deaths worldwide. The battle against this disease continues to challenge physicians and scientists daily, due to its various forms, treatments, and responses. Depending on the grade and stage of the disease, treatments can be extensive, encompassing surgery, chemotherapy, and radiation therapy. Though these therapeutics can prolong a patient’s life, they often have significant ramifications. For instance, radiation therapy has an extensive list of side effects, from organ damage to loss of fertility. These outcomes have driven scientists to explore the applicability of novel cancer treatments. Imidazolium and benzimidazolium salts have been shown …