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Life Sciences

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Masters Theses

2022

Climate Change

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Changes In Gene Expression From Long-Term Warming Revealed Using Metatranscriptome Mapping To Fac-Sorted Bacteria, Christopher A. Colvin Oct 2022

Changes In Gene Expression From Long-Term Warming Revealed Using Metatranscriptome Mapping To Fac-Sorted Bacteria, Christopher A. Colvin

Masters Theses

Soil microbiomes play pivotal roles to the health of the environment by maintaining metabolic cycles. One question is how will climate change affect soil bacteria over time and what could the repercussions be. To answer these questions, the Harvard Forest Long-Term Warming Experiment was established to mimic predicted climate change by warming plots of land 5℃ above ambient conditions. In 2017, 14 soil core samples were collected from Barre Woods warming experiment to mark 15 years since the establishment of the soil warming in that location. These samples underwent traditional metatranscriptomics to generate an mRNA library as well as a …


Climate-Driven Selection Results In Powerful Geographic Framework For Predicting Phenotype, Alexandra Neild May 2022

Climate-Driven Selection Results In Powerful Geographic Framework For Predicting Phenotype, Alexandra Neild

Masters Theses

Our ability to prepare for and mitigate the likely ecological and evolutionary impacts of climate change largely depends upon our ability to predict the phenotypic responses of organisms that allow them to persist, adapt, and migrate along environmental stress gradients. Using fifteen populations of cottonwoods, a dominant riparian forest tree, that are distributed along elevation gradients and represent three genetic provenances, we hypothesized and show that: 1) populations within a provenance demonstrate parallel evolutionary responses to climatic gradients associated with elevation; and 2) the evolutionary effects of elevation on bud-break phenology varied by provenance. Across all populations, we find strong …