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Diet And Activity Patterns Of Five Bat Species In North-Central Kansas, Holly G. Wilson

Master's Theses

My study focuses on six bat species that occur in north-central Kansas. Although each species is widely distributed, information about their diet and activity patterns is lacking, especially within Kansas. Increased knowledge about bat species in Kansas can provide a baseline for future studies and conservation efforts for the species included in my study; big brown bat (Eptesicus fuscus), eastern red bat (Lasiurus borealis), hoary bat (Lasiurus cinereus), northern myotis (Myotis septentrionalis), evening bat (Nycticeius humeralis), and tricolored bats (Perimyotis subflavus) were captured and fecal samples were examined for diet diversity. I captured bats in mist nets in the Kansas …

The Role Of Orphan Nuclear Receptor Dax-1 (Nr0b1) In Human Breast Cancer Cells: Expression, Proliferation And Metastasis, Erin Dishington

Master's Theses

The orphan nuclear hormone receptor DAX-1 (Dosage Sensitive Sex Reversal, Adrenal Hypoplasia Congenita on the X Chromosome, gene 1) plays an important role in the development of adrenal and gonadal tissues and functions as a global negative-regulator of steroidogenesis. In addition, it is known to be involved in several diseases including some cancers. Herein, we describe our examination of the role of DAX-1 in breast cancer, specifically its influence on proliferation and metastasis and its expression during progressive stages of disease. In an effort to understand how DAX-1 influences breast cancer cell proliferation and metastasis, we used MCF7 breast cancer …

A Neuroprotective Role For Mir-1017, A Non-Canonical Mirna, Matthew De Cruz

Master's Theses

miRNAs are post-transcriptional regulators of gene expression, with numerous being involved in neurobiology. Within the human genome a quarter of the identified miRNA loci derive from a class of miRNAs termed tailed mirtrons. Despite the identification of this large population of miRNA, no functional studies have been conducted to identify their role. In this study we examined the highly expressed and deeply conserved Drosophila 3’ tail mirtron, miR-1017, as a candidate to elucidate tailed mirtron functionality. We identified acetylcholine receptor transcripts, Da5 and Da2, as bona fide targets for miR-1017. Interestingly, Da2 is also the host transcript for miR-1017. We …

Impacts Of Oil Exposure During Early Life Development Stages In Sheepshead Minnows (Cyprinodon Variegatus) Under Different Environmental Factors, Danielle Simning

Master's Theses

The release of approximately 5 million barrels of crude oil into the northern Gulf of Mexico during the 2010 Deepwater Horizon oil spill jeopardized estuarine ecosystem health from Texas to Florida. These estuarine habitats, which serve as nurseries for many important fisheries are also prone to rapid fluctuations in environmental stressors such as oxygen concentration, and salinity. The consequence of combined exposure to crude oil and suboptimal environmental factors during early life stage development of fish is still largely unknown. The objective of this project was to investigate the impacts of exposure to crude oil in combination with varying environmental …

Combined Effects Of Dissolved Oxygen And Temperature On Aerobic Respiration And Respiratory Recovery Responses Of The Spioniform Polychaete, Streblospio Gynobranchiata, In Relation To Body Size, Alyssa Bennett

Master's Theses

Elevated surface temperatures exacerbate the threat of hypoxia within coastal ecosystems. These two primary stressors likely interact as they elicit opposing physiological responses from marine organisms. Metabolic depression is typically associated with hypoxia, while metabolic rates increase with temperature. Moreover, physiological effects of combined stressors may not be additive. In light of increasing pressures from hypoxia, elevated ocean temperatures, and other stressors within coastal regions, studies need to examine effects of multiple stressors on physiology of coastal organisms.

Mass-specific aerobic respiration (V_O2) was characterized as a proxy for metabolic cost of Streblospio gynobranchiata, at combined levels of …

Effects Of Temperature On Growth And Molting In Blue Crabs (Callinectes Sapidus) And Lesser Blue Crabs (Callinectes Similis), Abigail Ann Kuhn

Master's Theses

Temperature can exert impacts on many processes in ectotherms. With global temperatures rising due to climate change, many ectothermic species may exhibit changes in growth rates and size at maturity, and these changes can have population-level effects. Predicting responses of species to climate change will require not only knowledge of thermal tolerance limits, but also effects of temperature change on growth rates and other life history parameters. For arthropods that exhibit discontinuous growth (i.e., molting), this includes both intermolt period and growth per molt. Previous laboratory and field experiments suggest that temperature affects both intermolt period (IMP) and growth per …

Influences Of Anthropogenic Noise On Flight Initiation Distance, Foraging Behavior, And Feeder Community Structure Of Wild Birds, Alissa R. Petrelli

Master's Theses

Throughout the world, birds represent the primary type of wildlife that people experience on a daily basis. However, a growing body of evidence suggests that alterations to the acoustic environment can negatively affect birds as well as humans in a variety of ways, and altered acoustics from noise pollution has the potential to influence human interactions with wild birds. In this thesis, I investigated how anthropogenic noise impacts daily behavior as well as community structure of wild birds. In the first component of this thesis, I assessed the distance at which a bird initiates flight or escape behavior (i.e., flight …

Forest Densification Over 85 Years In A Sierra Nevada Mixed-Conifer Forest Decreases Conifer Regeneration And Limits Survival, Marissa A. Vossmer

Master's Theses

Forest densification in response to a century of fire suppression in Sierra Nevada mixed conifer forests has decreased conifer regeneration and survival. Increases in overstory biomass and decreases in canopy heterogeneity, along with decreases in shrub cover in the understory, has created unfavorable establishment site conditions for conifer species. Establishment site conditions are key in promoting germination and establishment of conifers seeds and in determining the survival of these seedlings into the overstory. These changes in establishment site suitability resulting from the removal of disturbance from these forests has decreased conifer regeneration and survival into other age classes. In Sierra …

Assessing The Effect Of Nutritional Interventions On Body Composition Changes During Pregnancy In Moderately Malnourished Malawian Women, Megan K. Kitagawa

Master's Theses

Background: Malnutrition can affect the body composition changes that occur in healthy pregnancy, resulting in adverse effects to both the mother and infant. Supplementary foods with high quality protein may result in more favorable changes in lean mass and fat mass in pregnant malnourished women.

Objective: To retrospectively assess changes in body composition in moderately malnourished pregnant Malawian women in response to one of three nutritional interventions.

Methods: Height, weight, MUAC, and TSF were measured in pregnant women with MUAC >20.6 cm and19) were compared using ANOVA. If differences were detected, differences between groups were tested using the Tukey HSD …

Proteomic Analysis Of The Crustacean Molting Gland (Y-Organ) Over The Course Of The Molt Cycle, Talia B. Head

Master's Theses

Molting in crustaceans is a highly complex physiological process involving negative regulation by two paired endocrine glands, the X-organ/sinus gland complex (XO/SG) and the Y-organ (YO). The XO/SG complex is responsible for making molt-inhibiting hormone (MIH) which negatively regulates synthesis of the molting hormones, ecdysteroids, by the YO. Analysis of gene expression in the XOs and YOs has led to the development of a proposed molecular signaling pathway which regulates ecdysteroidogenesis and subsequent molting in crustaceans. In this study, changes in protein abundance in the YO were characterized over the course of a molt cycle (intermolt, early premolt, mid premolt, …

Environmentally-Driven Variation In The Population Dynamics Of Gulf Menhaden (Brevoortia Patronus), Grant D. Adams

Master's Theses

Gulf Menhaden (Brevoortia patronus) is an abundant forage fish distributed throughout the Northern Gulf of Mexico (NGOM). Gulf Menhaden support the second largest fishery, by weight, in the United States and represent a key linkage between upper and lower trophic levels. Variation in the population dynamics can, therefore, pose consequences for the ecology and economy in the NGOM. Here we aim to understand variation in the individual and population dynamics of Gulf Menhaden throughout ontogeny and how such variation relates to environmental processes. We utilized a suite of fishery-dependent and –independent, remote sensing, modeled, and in situ data …

Evidence Of Multidecadal Recruitment In The Ocean Quahog, Arctica Islandica In The Western Atlantic Ocean, Sara M. Pace

Master's Theses

Ocean quahogs (Arctica islandica) are the longest-lived, non-colonial animals known today, with a maximum life span exceeding 500 years. Limited information is available regarding recruitment, making the sustainable management of this valuable fishery a challenge. The objective of this research was to describe the age structure and growth rates for four populations of ocean quahogs from the mid-Atlantic stock to evaluate long-term recruitment trends. Clams were sectioned for age estimation to develop population age frequencies. Initial colonization began approximately 175-250 years ago depending upon site. All sites experienced an increase in recruitment beginning in the late 1800’s to …

Effects Of Wave Action On The Structure Of Fish Assemblages Across An Exposure Gradient, Lauren Liddon

Master's Theses

Disturbance affects the function and diversity of ecosystems. Increased wave exposure to salt marsh can disturb sediments and cause a loss of habitat. The purpose of this study was to explore the effects of increased wave exposure on diversity, abundance, and functional ecology of estuarine fishes. If increased wave exposure is acting as a disturbance to these habitats, ecological theory (Intermediate Disturbance Hypothesis) predicts that diversity will peak at intermediate frequencies and intensities of disturbance. Fish were sampled from 10 sites monthly for 6 years. The sites were assigned to different exposure categories (Open, Intermediate, and Sheltered) using an exposure …

Strategies For Increasing The Release Of Pigments In Red Wine, Briana Heywood

Master's Theses

The perception of wine’s quality is directly influenced by its color. Anthocyanin molecules are responsible for imparting color to red wines. They are extracted from grape skins during alcoholic fermentation. This work compares the effects of three parameters: berry integrity, enzyme addition, and fermentation temperature, on phenolic compound extraction (total phenol, tannin and anthocyanin) during the production of Paso Robles’ Cabernet Sauvignon wine. Analyses on phenolic compounds were completed during alcoholic fermentation and barrel aging over the course of eighteen months. Berry integrity compared the degree of berry crushing (whole destemmed berries versus fully crushed berries). Results showed that phenolic …

A Comparative Analysis Of The Nutrient Composition And Digestibility Of California Perennial And Annual Grasses At Four Stages Of Growth, Elaina Cromer

Master's Theses

Beef products represent the fourth largest agricultural commodity in the state of California, valuing more than $3 billion from 2013 to 2015 (CDFA, 2016) and procure 90% of the income for the range livestock industry (FRAP, 2003). Forages found on California’s coastal, desert, foothill, and mountain ranges are the basis of the state’s beef cattle industry. Understanding their nutritional quality of these forages is important for their effective use (George et al., 2001a; Waterman et al., 2014). The objectives of this research were to investigate the nutritional characteristics, and in situ digestbilities in Angus beef cattle, of common California annual …

The Recovery Of Protein From Egg Yolk Protein Extraction Granule Byproduct, Irene Jennifer Kaufman

Master's Theses

In addition to proving an excellent source of nutrients, eggs are used in the food, cosmetic, and biotechnology industries for their rheological and bioactive properties. Much of the potential for the added value is in individual components of the egg, rather than the whole egg. At low speed centrifugation, yolk separates into two distinct fractions—granules and plasma. It is becoming increasingly popular in the industry to remove the plasma fraction of the egg yolk to use for its livetins, particularly immunoglobulin Y, leaving behind a granule by-product (“yellow cake”). Previous research has shown potential added-value from the granule fraction, especially …

Pink And Dude Chefs: Efficacy Of An Online Train-The-Trainer Mechanism And Student Program Outcomes, Jacqueline Chen

Master's Theses

Background: The rate of overweight and obesity among adolescents aged 12-19 years has quadrupled since 1980. Reducing obesity is a key public health priority, as obesity is associated with individual and population-level health and economic consequences. Afterschool-based obesity prevention programs that aim to develop nutrition and culinary self-efficacy have shown promise. However, the level of aptitude among program instructors is directly correlated with student success, suggesting the importance of effective train-the-trainer (TTT) mechanisms for implementing and scaling up these strategies.

Pink and Dude Chefs (PDC) is an afterschool nutrition education and culinary skills program for middle-school adolescents aged between 11-14 …

Effects Of Food Consumption On Cell Proliferation In The Brain Of Python Regius, Stacy Star Habroun

Master's Theses

Neurogenesis is an important and vastly under-explored area in reptiles. While the ability to generate new brain cells in the adult mammalian brain is limited, reptiles are able to regenerate large populations of neuronal cells. Pythons exhibit a characteristic specific dynamic action (SDA) response after food intake with an increase in metabolic rate that facilitates processing the meal. Associated with this change in SDA, pythons (Python spp.) also exhibit impressive plasticity in their digestive and cardiovascular physiology due to the sheer magnitude of the increase in organ growth that occurs after a meal to speed digestion, absorption, and assimilation of …

The Population Genetics Of Morro Bay Eelgrass (Zostera Marina), Julia Gardner Harencar

Master's Theses

Seagrass populations are in decline worldwide. Zostera marina (eelgrass), one of California’s native seagrasses, is no exception to this trend. In the last 8 years, Morro Bay, California has lost 95% of its eelgrass. Eelgrass is an ecosystem engineer, providing important ecosystem services such as sediment stabilization, nutrient cycling, and nursery habitats for fish. The failure of recent restoration efforts necessitates a better understanding of the causes of eelgrass decline in this estuary. Previous research on eelgrass in California has demonstrated a link between population genetic diversity and eelgrass bed health, ecosystem functioning, and resilience to disturbance and extreme climatic …

Effects Of Ankle Weights On Metabolic Response And Muscle Activity On A Lower Body Positive Pressure Treadmill 2017, Saige Hupman

Master's Theses

Lower body positive pressure (LBPP) treadmills are growing in popularity for rehabilitative use, as the benefits of exercising at partially supported body weight may induce faster recovery. It is unknown if there are certain practices that increase exercise intensity while maintaining positive effects of LBPP. Adding ankle weights when walking or running could increase intensity of rehabilitation programs while maintaining the comfort of supported body weight. PURPOSE: To measure metabolic response (VO2, RER, HR, Caloric expenditure), RPE, and lower limb electromyography (EMG) amplitudes of LBPP treadmill walking and running with and without ankle weights. METHODS: Sixteen participants (Age: 21.94 ± …

Do Pinnipeds Have Personality? Coding Harbor Seal (Phoca Vitulina) And California Sea Lion (Zalophus Californianus) Behavior Across Contexts, Amber J. De Vere

Master's Theses

Personality has now been studied in species as diverse as chimpanzees (King & Figueredo, 1997) and cuttlefish (Carere et al., 2015), but marine mammals remain vastly underrepresented in this area. A broad range of traits have been assessed only in the bottlenose dolphin (Highfilll & Kuczaj, 2007), while consistent individual differences in a few specific behaviors have been identified in grey seals (Robinson et al., 2015; Twiss & Franklin, 2010; Twiss, Culloch & Pomeroy, 2011; Twiss, Cairns, Culloch, Richards & Pomeroy, 2012). Furthermore, the context component of definitions of personality is not often assessed, despite evidence that animals may show …

Sparking A Dolphin's Curiosity: Individual Differences In Dolphins' Reactions To Surprising And Expectation-Violating Events, Malin Katarina Lilley

Master's Theses

Non-scientific literature consistently describes dolphins as “curious animals,” but there has been little systematic research on curiosity in dolphins. Curiosity in humans and certain non-human animal species, including birds and non-human primates, has been studied by examining individual differences in exploration and reactions to novel stimuli. Additionally, research has explored how human infants and non-human animals react when an event violates their expectations. The present study explored dolphins’ reactions to spontaneously surprising and expectation-violating stimuli. The reactions of dolphins, 15 bottlenose (Tursiops truncatus) and 6 rough-toothed (Steno bredanensis), at Gulf World Marine Park were analyzed in …

Age, Growth, And Reproduction Of Vermilion Snapper (Rhomboplites Aurorubens) In The North-Central Gulf Of Mexico, Trevor Dalton Moncrief

Master's Theses

Vermilion Snapper is a commonly harvested species of reef fish in the northern Gulf of Mexico (GOM). It supports both a large commercial and popular recreational fishery, however knowledge of this fish’s life history is limited spatially. Non-linear curve fitting was used to estimate growth parameters and Akaike information criteria (AIC) was used to determine relative model fit. The 2-parameter von Bertalanffy growth function provided the best model fit and lowest AIC score. Histological examination indicated that Vermilion Snapper are batch spawners with asynchronous oocyte development. Additionally, 17% of Vermilion Snapper in the actively spawning phase containing 24 hour POF’s …

The Effect Of Boat Type On Bottlenose Dolphin (Tursiops Trucatus) Behavior In The Mississippi Sound, Maria Zapetis

Master's Theses

Increases in oceanic shipping are a global phenomenon, and a leading cause of concern for marine animal welfare. While it may be difficult to assess the effect of boat traffic on all species in all contexts, it is vital to report anthropogenic impacts where longitudinal data is available, and doubly so where a dearth of information exists. The purpose of this study is to describe how dolphin behavior changed in the presence of boats in the Mississippi Sound between 2006 and 2012, and more specifically, to detail how different boat types impacted dolphins’ behavioral states. This study is unique in …

Life History Of The Non-Native Invasive Red Lionfish (Pterois Volitans) In The Northern Gulf Of Mexico, Alexander Q. Fogg

Master's Theses

Invasive Red Lionfish (Pterois volitans) were first detected in the northern Gulf of Mexico (nGOM) in 2010 and since then their numbers have increased dramatically. From 2010 to 2015, more than 15,000 Red Lionfish were collected opportunistically from the nGOM for this study. Length and weight relationships differed significantly among ecoregions by sex and there was clear sexual dimorphism in size with males being larger and heavier. Red Lionfish age ranged from 0-4.5 years old and males achieved greater growth rate (K) and asymptotic maximum lengths (L_inf) compared to females and these parameters were also different …

Characterization Of Andrenocortical Tissue Morphology, Histology, And Steroid Synthesis Among Finetooth (Carcharhinus Isodon), Blacktip (Carcharhinus Limbatus), Atlantic Sharpnose (Rhizoprionodon Terraenovae), And Bonnethead (Sphyrna Tiburo) Sharks, Danielle Elizabeth Bailey

Master's Theses

Adrenocortical (interrenal) tissue is composed of steroidogenic cells that produce corticosteroids involved in the stress response and hydromineral balance. Previous research characterizing the elasmobranch interrenal suggests that the number of interrenal bodies that produce the single primary corticosteroid, 1a-hydroxycorticosterone (1a-OHB), varies among species. However, potential species-specific differences in the amount of interrenal tissue and major steroid products produced have been understudied. To address this critical gap in our understanding of elasmobranch biology, this study examined interrenal morphology, cell structure, steroidogenic enzyme distribution (3bHSD), and steroid production in four shark species: Finetooth Carcharhinus isodon, Blacktip Carcharhinus limbatus, Atlantic Sharpnose …

Phylogenetic Distribution Of An Endogenous Strain Of Dahlia Mosaic Virus In Members Of Asteraceae, Keri L. Caudle

Master's Theses

A newly discovered strain of Dahlia mosaic virus (DMV) called DMV-D10 was first observed in Dahlia variabilis in 2008. DMV-D10 does not induce visible symptoms of infection in the host plant, and is classified as an endogenous virus. Endogenous viruses like DMV-D10 have the ability to integrate their viral sequences into the host plant genome, which can be transmitted to offspring. No studies have examined the host range of DMV-D10 outside of the Dahlia genus. Because DMV-D10 has only been observed in Dahlia, the objective for this study was to determine if presence of DMVD10 follows an evolutionary relationship among …

Post-Drought Survey Of Freshwater Mussels In The Saline And Smoky Hill Rivers With Emphasis On The Status Of The Cylindrical Papershell (Anodontoides Ferussacianus) And Effects Of Lowhead Dams On Growth Of The Pimpleback (Quadrula Pustulosa) In The Neosho River, Kansas, Andrew T. Karlin

Master's Theses

Study 1:Post-drought survey of freshwater mussels in the Saline and Smoky Hill rivers with emphasis on the status of the Cylindrical Papershell (Anodontoides ferussacianus) in Kansas. The Cylindrical Papershell (Anodontoides ferussacianus), considered a “Species in Need of Conservation” in Kansas, historically occurred across much of the state; however, recent studies suggest that the species is currently restricted to the upper Smoky Hill-Saline River Basin, and a survey emphasizing the status of the Cylindrical Papershell conducted in 2011 suggested its conservation status be elevated to endangered. Continuing drought since the completion of the 2011 survey raised concerns regarding the status of …

Soxhlet Extraction Of Avocado Endocarp And Trituration Of Avocado Mesocarp For Biodiesel Production., Ifeoma Oraemesi

Master's Theses

Finding alternative sources of renewable energy is on the rise globally. Renewable sources of energy are advantageous because they are biodegradable, less toxic, and combust efficiently. More importantly, raw materials for these sources can be replenished. One alternative source of energy is biodiesel. Biodiesel is a fuel which consists of mono-alkyl esters of long-chained fatty acids obtained from vegetable oil or animal fats. They serve as efficient fuels to run diesel engines. Biodiesel is produced via transesterification of oils wherein glycerin is a by-product. Avocado (Persea americana) is a fleshy fruit with high lipid content, mostly monounsaturated fats, which amounts …

Using Maximum Entropy Species Distribution Modeling For Long-Term Conservation Planning Of Three Federally Listed Bats In North America, Mitchell L. Meyer

Master's Theses

We are currently in a sixth mass extinction event in which the extinction rate is higher than it has ever been. This mass extinction event is caused by human influence on the environment. Biodiversity is worth conserving because of its many uses to humans. Bats are a diverse group of mammals that humans rely on for pest control services. The gray bat, northern long-eared bat, and Indiana bat are on the Threatened and Endangered Species List and are in need of conservation. I built species distribution models using occurrence records, climate data, and Maximum entropy (MaxEnt) modeling technique. I predicted …