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The Role Of Rna Editing Within Calcium-Dependent Activator For Secretion 1 In Large Dense Core Vesicle Fusion, Kaylee M. Mathiason

MSU Graduate Theses

Calcium-dependent activator protein for secretion 1 (CAPS1) is involved in synaptic vesicle and large dense core vesicle fusion by bridging vesicles and the plasma membrane prior to vesicle fusion and cargo release. CAPS1 is subject to a post-transcriptional modification called RNA editing. RNA editing recodes a single amino acid in the region of the protein that interacts with the vesicle. This recoding event within CAPS1 has cellular consequences in neurons where edited CAPS1 overexpression increases basal secretion from neural synaptic vesicles. Our goal was to investigate how RNA editing within CAPS1 affects large dense core vesicle secretion in an insulinoma …