Digital Commons Network^™

Comparing Calcium, Vitamin D, And Calorie Intakes Among Unh Army Rotc Cadets With Low And Normal Bone Mineral Status, Ava Gaudette

Honors Theses and Capstones

No abstract provided.

Investigation Of The Effects Of Ocean Acidification On Juvenile Homarus Americanus Feeding Behavior, Sophia Roy

Honors Theses and Capstones

Climate change has led to increased CO2 absorption by ocean surface waters, resulting in decreased pH levels, a phenomenon known as ocean acidification (OA). A primary class of marine organism behavior is impacted by OA, in particular, olfactory-mediated behaviors related to foraging and feeding success. However, the biological mechanisms responsible for the documented impairment of foraging and feeding-related behaviors remains in question. We find that juvenile American lobsters (H. americanus) exhibit significantly faster rates of total feeding times in response to short-term low pH exposure. However, total search time and total handling time were not affected by pH when examined …

Equine Rescue Success In The United States: A Statistical Analysis, Carly J. Cave

Honors Theses and Capstones

The high inflation that has characterized the post-pandemic period in the United States has increased the costs of horse ownership for private owners and equine rescue organizations, which are facing critical pressures. Over 200,000 equines are at risk for neglect or slaughter in the United States each year, which far exceeds the capacity to house these equines at rescue organizations. While demand on these organizations is high, their ability to afford appropriate resources for their care is insufficient. This study examined geographical and economic factors that impact equine rescues’ success in taking in at-risk equines and adopting them to forever …

Financial Considerations For U.S. Equine Rescues: A Comparison Of Adoption-Based Vs. Sanctuary-Based Organizations, Carolyn V. Frank

Honors Theses and Capstones

No abstract provided.

Prospective Associations Of Fish Consumption In Cognitive Function And Depressive Symptoms Among Puerto Rican Adults, Johanna Grace Koroma

Honors Theses and Capstones

Johanna Koroma, Anne Bodenrader, and Sherman Bigornia

Objectives

Fish intake has been linked to certain neural benefits, specifically in slowing cognitive decline and attenuating depressive symptoms. However, existing evidence is conflicting and insufficient. The present study aimed to investigate the association of fish consumption with depressive symptoms and cognitive function among Puerto Rican adults.

Methods

Data is acquired from the Boston Puerto Rican Health Study, comprised of 1517 adults residing in the Boston Metro Area. Dietary patterns were evaluated using a validated food frequency questionnaire (FFQ) with follow-up surveys conducted 2 years from baseline. Consumption was organized into quartiles based …

Utilizing Plasmid Dna Hydrogels For Cell-Free Protein Production, Tinotenda Duche

Honors Theses and Capstones

Proteins play an important role in various biological processes and are crucial components in biomedical research and therapeutics. Cell-free protein synthesis (CFPS) has provided a valuable approach for rapid protein production outside living cells, involving both transcription and translation processes. However, traditional CFPS methods encounter challenges such as high cost, relatively low yields, and scalability. The end goal of our project is to incorporate our template into the hydrogel. In this work, we report advancements towards that by trying to optimize our solution of CFPS. Unfortunately, the standards for pDNA hydrogel synthesis are not met by the pDNA production and …

Distribution Of Tick Species On Free-Ranging Ungulates In Northern New England, Karisa Graham

Honors Theses and Capstones

In Northern New England, ungulates are often parasitized by ticks, which is one of the leading causes for the decline in population. Ungulates are a good host for ticks, specifically deer ticks and winter ticks, and these ticks cause many tick-borne diseases in humans as well. The purpose of this study was to assess passive sampling from harvested animals as a means of tick surveillance in Vermont and New Hampshire. Ticks were collected from deer and moose and mapped throughout the two states by ArcGIS to visualize the trends in distribution. Relative abundance was greater in southeastern Vermont, with no …

Alpaca Assisted Activities In The New England Alpaca Industry: A Possible Solution To Farm Profitability And Participant Mental Health, Ashley Michele Masse

Honors Theses and Capstones

The high operating costs of the New England alpaca industry were putting the majority of alpaca farmers in this region in a state of financial loss. On a separate note, American communities were experiencing a prevalence of anxiety and depression. What if a single solution could help improve the outcome of both these challenges that seem to have little to do with each other? Alpaca Animal Assisted Activities (alpaca AAAs) was a possible solution. This study explored New England alpaca farm profitability and alpaca AAAs in a way no other research study has done before. A Farmer Survey was mailed …

Unsung Heroes In Conservation: Evaluating The Limitations Faced By New England Wildlife Rehabilitation Centers And Proposing Solutions For Their Support And Recognition, Shaylee M. Sarmiento

Honors Theses and Capstones

Wildlife rehabilitation is an often-unacknowledged practice within the overarching field of wildlife protection and conservation. This practice involves the treatment and hopeful release of wild animals affected by various ailments. With the continued expansion of and interest in the field, it could be assumed that wildlife rehabilitation is supported and funded by government bodies, but this is not the case. Because of the lack of funding and resources, many wildlife rehabilitation centers cannot achieve their full rehabilitative potential and expand their reach in the conservation world. Additionally, an absence of support leads critics to question the ethical standards of wildlife …

Assessment Of Open Top Chambers To Simulate Effects Of Climate Change On Soil Temperature And Cover Crop Response In Agricultural Systems, Gretchen Bahmueller

Honors Theses and Capstones

Climate change is expected to yield warmer winters that have the potential to place additional stress on our already stressed agricultural systems. Understanding how agricultural systems may respond to these changes is essential to creating crop and land management plans that ensure food security for future generations. To better understand how warming winters can/will affect air and soil temperatures and cover crop performance, open top chambers (OTCs) were deployed post cover crop seeding in a field experiment at the UNH Kingman Research Farm in Madbury, NH. The experiment consisted of four cover crop treatments sown into or after corn: an …

Density Dependent Calling Behavior In North Pacific Humpback Whales, Lucas J. Anderson

Honors Theses and Capstones

Animal density can define factors such as mating success, food acquisition, and communication. Inferring animal density from vocal rates is an important tool for estimating abundance and space use in difficult-to-study species like marine mammals and requires an understanding of the relationship between social context and calling rates. The goal of this project was to investigate the relationship between animal density and call rate in Southeast Alaskan humpback whales (Megaptera novaeangliae). Passive acoustic monitoring data were collected along with visual observations gathered near Frederick Sound and Stephen’s Passage, Southeast Alaska, over two summers (2019, 2022). Observers watched whales …

Alpaca Assisted Activities In The New England Alpaca Industry: A Possible Solution To Farm Profitability And Participant Mental Health, Ashley Michele Masse

Honors Theses and Capstones

The high operating costs of the New England alpaca industry were putting the majority of alpaca farmers in this region in a state of financial loss. On a separate note, American communities were experiencing a prevalence of anxiety and depression. What if a single solution could help improve the outcome of both these challenges that seem to have little to do with each other? Alpaca Animal Assisted Activities (alpaca AAAs) was a possible solution. This study explored New England alpaca farm profitability and alpaca AAAs in a way no other research study has done before. A Farmer Survey was mailed …

Do Duckweeds Adapt To Water And Microbes?, Ava M. Rose

Honors Theses and Capstones

Organisms in their home environment sometimes outperform transplanted individuals native to other areas, in a phenomenon termed local adaptation. While local adaptation is traditionally considered to be driven by the abiotic factors of an environment, scientists have recently increased consideration of biotic factors. Specifically, of interest to many is host-associated microbiomes, which can alter host trait expression. As the desire to commercialize microbiome treatments for agriculture and medicine grows, it is important to analyze the potential value of local microbiomes, which may be adapted to their hosts, or to which local hosts may have adapted. Using Lemna minor (duckweed) as …

The Continuation Of Thoroughbred Racing: A Dual Mission, Isabella V. Williams

Honors Theses and Capstones

Thoroughbred racing is the highest-profile segment of the equine industry in the United States and around the world, accounting for 50% of industry jobs and 25% of industry revenue. Public interest in the sport is rising thanks to social media and celebrity involvement at marquee events like the Kentucky Derby, leading to increased scrutiny of the sport, posing a high risk to human and equine participants alike. Public outcry at equine injuries in particular threatens the sport’s social license to operate (SLO), making it critical to protect the “whole-life welfare” of the animals involved in the sport to ensure its …

The Influence Of Drd2 Polymorphism Exon 8 C/T (Rs6276) On Manifestations Of Delirium Tremens & Alcohol Withdrawal Seizures, Naomi Schneider

Honors Theses and Capstones

This study explores the correlation between the DRD2 Polymorphism exon 8 C/T (rs6276) and manifestations of delirium tremens (DT). DT is a condition that is clinically diagnosed utilizing two characteristic symptom manifestations: the presence of delirium and severe alcohol withdrawal. It is not entirely understood why DT can occur in some patients, but evidence has suggested that genetic predisposition can play a role. Utilizing the National Institutes of Health (NIH) All of Us Research database and performing a secondary analysis of existing genomic data, this candidate gene association study aims to determine the genotype frequencies within three cohorts: a healthy …

Impact Of Hdac Inhibitors On The Efficiency Of Cardiomyocyte Differentiation, Lauren Mary Sargent

Honors Theses and Capstones

Heart disease and injury is the leading cause of death worldwide, with much of its severity caused by the low regenerative capacity of cardiomyocytes (CMs). One of the strategies to counteract the poor regeneration of CMs is the production of CMs via differentiating stem cells. However, the low efficiency of obtaining CMs from stem cells is an issue because there are numerous inconsistencies in the process. Histone deacetylase (HDAC) inhibitors have been researched as ways to increase efficiency of CM differentiation by increasing histone acetylation, and thereby allowing differentiation to occur more efficiently. In this study, the HDAC inhibitor, trichostatin …

Investigating The Function Of Protein S-Acyltransferase 2 In Arabidopsis Thaliana Using Crispr Mutants, Nicole Benjamin

Honors Theses and Capstones

Arabidopsis thaliana contains 24 Protein Acyl Transferases (PATs). These enzymes are responsible for catalyzing the addition of a palmitate to a target substrate (palmitoylation). However, beyond its broad enzymatic function, it is unknown how PATs interact on a cellular level. This research focuses on identifying the function of PAT2. CRISPR-Cas9 was used to create deletion mutations that would result in a non-functional PAT. Two plants homozygous for a pat2 deletion were identified: pat2-5 and pat2-6. Preliminary results suggest that pollen grains in both pat2 mutants are larger in both length and width. Additionally, it appears that mutant pollen tends to …

Individual Behavioral And Neurobiological Markers Associated With A Vulnerable To Ethanol Use Phenotype, Kelsey M. Alimandi

Honors Theses and Capstones

Alcohol use disorder (AUD) is a chronic relapsing brain condition that is characterized by excessive alcohol consumption, continued use when faced with negative consequences, and a negative emotional state associated with withdrawals (anxiety, irritability, depression). The main challenge to treating AUD is preventing relapse. The purpose of this study was to use a prolonged-exposure model to allow rats to self-administer ethanol to determine the brain regions active during relapse events. The rats performed multiple behavioral tests such as economic demand, negative consequences, and an elevated plus maze. These tests determined how hard rats were willing to work for an ethanol …

Targeting Stat3 In Triple Negative Breast Cancer Using Gsk3Ꞵ And Integrin Inhibitors, Emily A. Pratt

Honors Theses and Capstones

Triple negative breast cancer (TNBC) is the most aggressive subtype of breast cancer and is known for its chemoresistance and high rate of recurrence. TNBC lacks the hormone receptors that are found in other types of breast cancer, and therefore cannot be treated with hormonal therapies. Although the initial response to chemotherapy is favorable, many TNBC patients experience a recurrence of this cancer at a secondary location and this recurrence is often more lethal than the original tumor. The Signal Transducer and Activator of Transcription (STAT) pathway is a major contributor to cancer growth and metastasis, and STAT3 is constitutively …

Neuronal Primary Cilia In Postnatal Brains & Alzheimer’S Disease Mice Eeg Patterns Under Fear Conditioning, Sierra Rose Mae Walsh

Honors Theses and Capstones

Alzheimer’s Disease (AD) is a neurodegenerative condition caused by the abnormal accumulation of amyloid β plaque. While small amounts of amyloid β plaque is to be expected with increased age, AD presents amyloid precursor protein (APP), a gene promoting inappropriate plaque formation, causing early neuronal death and tissue depreciation. APP23 transgenic mouse models contain the human mutation in the neocortical and hippocampal positions, areas revealing of plaque accumulation and memory loss. Within the hippocampal cortices, the primary cilia, which regulate higher cognition and neurodevelopment can be studied along with burst suppressions, or moments of high focal activity, to target potential …

Identification Of Novel Biosynthetic Gene Clusters Encoding For Polyketide/Nrps-Producing Chemotherapeutic Compounds From Marine-Derived Streptomyces Hygroscopicus From A Marine Sanctuary, Hannah Ruth Flaherty

Honors Theses and Capstones

Nearly one out of six deaths in 2020, around ten million people, were caused by cancer, making it a leading cause of death worldwide (WHO, 2022). This major public health issue, in addition to the rise of multidrug-resistant (MDR) pathogens, provides a high demand for the discovery of new pharmaceutical drugs to be used clinically to treat these conditions. The Streptomyces genus accounts to produce 39% of all microbial metabolites currently approved for human health, indicating its potential as an important species to study for antimicrobial and anticancer agents. The long linear genome of Streptomyces contains specialized sequences known as …

Revolutionary Advances In The Treatment Of Genetic Disease, Emma Kaitlyn Carrigan

Honors Theses and Capstones

No abstract provided.

Eeg-Based Spanish Language Proficiency Classification: An Eeg Power Spectrum And Cross-Spectrum Analysis, Blaise Xavier O'Mara, Skyler Baumer

Honors Theses and Capstones

Second language proficiency may be predicted with electrophysiological techniques. In a machine learning application, this electrophysiological data may be used for language instructors and language students to assess their language learning. This study identifies how electroencephalogram (EEG) power spectrum and cross spectrum data of the brain cortex relates to Spanish second language (L2) proficiency of 20 Spanish language students of varying proficiency levels at the University of New Hampshire. The two metrics for assessing cortical power and processing were event-related desynchronization (ERD)—a measure of relative change in power—of the alpha (8-12 Hz) brain frequency band, and alpha and beta (13-30Hz) …

Investigation Of The Function Of Protein Acyl Transferases Using Crispr-Cas9 To Create Null Mutants, Claire E. Christopher

Honors Theses and Capstones

24 Protein Acyl Transferases (PATs) have been identified in the model organism Arabidopsis thaliana. Despite knowing the enzymatic function of these genes (palmitoylation), the specific subcellular pathways, protein targets, and cellular activities of these proteins remain unknown. To investigate the pathways that PAT genes are involved in, deletion mutations were created in several genes and the phenotype of the organisms carrying homozygous mutations was observed. To introduce mutations, the CRISPR/Cas9 system was inserted into the plant’s genome using Agrobacterium tumefaciens to cause deletions in PAT genes of interest. To date, no CRISPR-induced pat mutants have a noticeable phenotype. These …

Dog Weight, Age, And Sex Relationship With Shelter Length-Of-Availability At Pope Memorial Humane Society, Logan Lajoie

Honors Theses and Capstones

Many factors can influence length-of-availability for dogs in shelters, ranging from factors of the dogs themselves to outside factors. The external factors could be the geographical location of the shelter and its clientele, the socioeconomic status of the area, how the individual shelter uses its resources, the region's policies, and more (Scarlett, 2021, p. 18). This study evaluated length-of-availability in dogs at the Pope Memorial Humane Society, a nonprofit animal shelter that serves Strafford County, New Hampshire. This allowed for dog-specific factors to be investigated more closely. The specific factors investigated were dog weight, age, and sex compared to their …

Odd Bedfellows & Marriage Of Necessity: Public Health And Politics In American Federalism, Gabriella Victoria Chianese

Honors Theses and Capstones

In the wake of the coronavirus disease 2019 (COVID-19) pandemic, while the public disagrees over mask mandates, required vaccinations, and social distancing, it seems like one common sentiment exists – a distaste for the odd bedfellows of politics and public health. There are those who cry for the compartmentalization of the science of public health and the art of politics to rectify this situation. In the wake of so much confusion and chaos, it is not unjustified to demand the depoliticization of public health; however, this response is unrealistic given the modern political climate, demonstrates a narrow understanding of the …

Assessing The Bioavailability Of Infused Lysine And Rumen-Protected Lysine Supplements Using The Area Under The Curve Technique And The Plasma Free Amino Acid Dose-Response Method, Megan Vetter

Honors Theses and Capstones

The milk production of lactating dairy cows is dependent on factors such as housing conditions, lineage, climate, and health, but the quality of their diets is generally the most influential. Maintaining a proper balance of nutrients is necessary to achieve the greatest milk production at the lowest cost. Maximum feed efficiency is not only critical for increasing the economic profits of an individual farm, but also for increasing food supply without increasing environmental demand. Supplementing cows’ diets with lysine (Lys), an essential amino acid (AA), can aid in maximizing protein synthesis. Providing this nutrient in a rumen-protected (RP) coating can …

Cutaneous Microbiome Of Red Fox (Vulpes Vulpes) Infected With Sarcoptic Mange (Sarcoptes Scabiei), Jacqueline C. Robidoux

Honors Theses and Capstones

Sarcoptic mange is a parasitic skin disease that affects countless mammals worldwide, including red foxes (Vulpes vulpes). The symptoms, such as hair loss, crusting, and the number of mites, vary between individual foxes. Sarcoptic mange damages the skin barrier, which in turn disrupts the biodiversity of bacteria in the microbiome. It is unknown what the biodiversity of bacteria is at each stage of the disease. This experiment will compare the microbiomes of different samples with and without mange; in hopes to reveal a connection between the different severities of sarcoptic mange and the biodiversity of bacteria and fungi …

Nutritive Comparison Of Ruminant Feed, Integrating Crab And Lobster Meal, Kelsi L. Devolve

Honors Theses and Capstones

In attempts to increase livestock production and decrease waste products from the seafood industry, crab meal and lobster meal were tested as a potential cattle feed ingredient. Jonah crab waste, Atlantic Red crab waste, soybean meal, and blood meal were collected from various vendors and utilized in an in vitro study, an in situ study, and a nitrogen analyzer to determine crude protein in each feed. After allowing the samples to sit in a DAISY incubator for 48 h, mimicking a rumen environment, degradability for the soybean meal, blood meal, lobster meal, and crab meal were 100.13%, 76.36%, 43.77%, and …

Taxonomic Classification Of Viral And Bacterial Dna Following 2021 Avian Mass Mortality Event, Tessa Baillargeon

Honors Theses and Capstones

From May through July 2021, an unusual mortality event occurred along the eastern coast and Midwest of the United States. Thousands of birds, mostly from the order Passeriformes, were part of the die-off including blue jays (Cyanocitta cristata), common grackles (Quiscalus quiscula), European starlings (Sturnus vulgaris), American robins (Turdus migratorius). Clinical signs included crusted eyes, swollen conjunctiva, otitis, seizures, and ataxia.

The New Hampshire Veterinary Diagnostic Laboratory (NHVDL) received over 100 affected birds from various collaborators throughout the United States including Washington DC, NJ, CT, MD, and OH. Given the timing and geologic …