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Improving Metabolic Engineering And Characterization Of Clostridium Thermocellum For Improved Cellulosic Ethanol Production, Beth Alexandra Papanek
Improving Metabolic Engineering And Characterization Of Clostridium Thermocellum For Improved Cellulosic Ethanol Production, Beth Alexandra Papanek
Doctoral Dissertations
Biofules are an important option for humanity to move away from its dependence on fossil fuels. Transitioning from food crops to lignocellulosic alternatives for the production of biofuels is equally important. Most commonly, biofuels are produced using a crop such as corn or soybeans to feed sugars to the yeast, Saccharomyces cerevisiae for the fermentation of ethanol. Lignocellulosic biofuel production would eliminate the need for food crops and transition to biomass such as switchgrass, poplar, or corn stover. Currently, lignocellulosic biofuel production is limited primarily because of the cost of converting the biomass to fermentable sugars than can then be …
Modification Of Carbohydrate Active Enzymes In Switchgrass (Panicum Virgatum L.) To Improve Saccharification And Biomass Yields For Biofuels, Jonathan Duran Willis
Modification Of Carbohydrate Active Enzymes In Switchgrass (Panicum Virgatum L.) To Improve Saccharification And Biomass Yields For Biofuels, Jonathan Duran Willis
Doctoral Dissertations
The natural recalcitrance of plant cell walls is a major commercial hurdle for plant biomass to be converted into a viable energy source as alternative to fossil fuels. To circumvent this hurdle manipulation of carbohydrate enzymes active in the cellulose and hemicellulose portions of the plant cell wall can be utilized to improve feedstocks. Production of cellulolytic enzymes by plants have been evaluated for reducing the cost associated with lignocellulosic biofuels. Plants have successfully served as bioreactors producing bacterial and fungal glycosyl hydrolases, which have altered plant growth to improve saccharification. A bioprospecting opportunity lies with the utilization of insect …
Conversion Of Cellulose To Ethanol By The Biofuels Microbe Clostridium Phytofermentans: Quantification Of Growth And Role Of An Rnf-Complex In Energy Conservation, Jesús G. Alvelo-Maurosa
Conversion Of Cellulose To Ethanol By The Biofuels Microbe Clostridium Phytofermentans: Quantification Of Growth And Role Of An Rnf-Complex In Energy Conservation, Jesús G. Alvelo-Maurosa
Doctoral Dissertations
The anaerobic mesophilic bacterium Clostridium phytofermentans grows and ferments multiple plant-based substrates into ethanol as the main product of fermentation. The capacity of C. phytofermentans to convert plant biomass into ethanol, propanol, and short-chain fatty acids is strongly attractive for industry. Specific physiological capabilities of C. phytofermentans allow the microbe to generate high amounts of ethanol compared to acetate. However, little is known about membrane energetics in C. phytofermentans, or its role in energy conservation and production of high levels of ethanol during fermentation of plant biomass substrates. In the first research project presented in this dissertation, we examined …