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Xenobiotic Response In Drosophila Melanogaster: Sex Dependence Of P450 And Gst Gene Induction, Gaelle Le Goff, Frederique Hilliou, Blair Siegfried, Sam Boundy, Eric Wajnberg, Luc Sofer, Pascaline Audant, Richard H. Ffrench-Constant, Rene Feyereisen
Xenobiotic Response In Drosophila Melanogaster: Sex Dependence Of P450 And Gst Gene Induction, Gaelle Le Goff, Frederique Hilliou, Blair Siegfried, Sam Boundy, Eric Wajnberg, Luc Sofer, Pascaline Audant, Richard H. Ffrench-Constant, Rene Feyereisen
Department of Entomology: Faculty Publications
The effect of xenobiotics (phenobarbital and atrazine) on the expression of Drosophila melanogaster CYP genes encoding cytochromes P450, a gene family generally associated with detoxification, was analyzed by DNA microarray hybridization and verified by real-time RT-PCR in adults of both sexes. Only a small subset of the 86 CYP genes was significantly induced by the xenobiotics. Eleven CYP genes and three glutathione S-transferases (GST) genes were significantly induced by phenobarbital, seven CYP and one GST gene were induced by atrazine. Cyp6d5, Cyp6w1, Cyp12d1 and the ecdysone-inducible Cyp6a2 were induced by both chemicals. The constitutive expression of several of the inducible …