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Is The Zebrafish Zombie Mutant Caused By A Mutation In Cdc20?, Peyton Johnston

Honors Theses

The zombie mutant was identified as an early arrest mutant, stopping in development around the 10-somite stage (14 hours of development). Further inspection revealed that this mutant was a cell cycle mutant and cells in the mutant arrest during metaphase as early as the 5-somite stage (11.6 hours of development). A similar phenotype is seen in the Drosophila melanogaster cell cycle gene fizzy, known to be to be a homolog of the Saccharomyces cerevisiae gene, cell division cycle 20 (cdc20). CDC20 is an activator protein of the anaphase promoting complex/cyclosome (APC/C), an ubiquitin E3 ligase that is …

A Comparison Of Gastrointestinal Bacterial Population Between Indoor Cats And Outdoor Cats, Farhana Binti Ikmal Hisham

Honors Theses

This study used fecal samples from four indoor cats and four indoor-outdoor cats in order to identify the microbial communities in the gut of cats. This information was then used to compare the microbial phyla between both groups for any differences. Total microbial DNA was isolated from each fecal sample, and the 16S rRNA gene was sequenced using Illumina MiSeq high throughput method. The sequences were identified using the bioinformatics program mothur. The results show that indoor cats had a more diverse microbial community as compared to outdoor cats. Indoor cat samples had 26% more microbial species, and eight more …

Expression Of Insulin Responsive Genes In Insulin Resistant Conditions, And The Effect Of Selenium On Gene Expression, David L. Ruff

Masters Theses

Chronically high blood glucose levels lead to many problems, such as insulin resistance, the hallmark of Type II diabetes. Increased flux through the hexosamine biosynthesis pathway is one mechanism by which high glucose as well as glucosamine has been shown to induce insulin resistance. This study tests the effects of glucosamine induced insulin resistance on insulin regulation of the metabolic genes glucose-6-phosphate dehydrogenase (G6PDH) and fatty acid synthase (FAS) as well as insulin responsive proteins tribbles homolog (TRIB3) and sterol regulatory element binding protein (SERBP-1c) 1c.

Selenium, a micronutrient has been shown to be an effective insulin mimetic in Type …

Investigations Of Filarial Nematode Motility, Response To Drug Treatment, And Pathology, Charles Nutting

Dissertations

More than a billion people live at risk of chronic diseases caused by parasitic filarial nematodes. These diseases: lymphatic filariasis, onchocerciasis, and loaisis cause significant morbidity, degrading the health, quality of life, and economic productivity of those who suffer from them. Though treatable, there is no cure to rid those infected of adult parasites. The parasites can modulate the immune system and live for 10-15 years. Testing of compounds against filarial nematodes is complicated due to a lack of an objective platform on which to analyze in vitro treatments. There is no published, immunocompetent laboratory model for lymphatic filariasis. This …

One Fish, Two Fish, Red Fish, Blue Fish: The Analysis Of The Specter Cell Cycle Mutant In Zebrafish, Tetiana Petrachkova

Masters Theses

Cell division is controlled by genes that regulate the cell cycle. Here we show that the zebrafish specter (spr) mutant is mutation in the cyclin B1 gene, a gene necessary for the G2 to M transition of the cell cycle.

The spr mutation mapped to the cyclin B1 gene. Sequencing showed a transition (C139→T) that caused a nonsense mutation in exon 2 of the cyclin B1 gene. In situ hybridization of cyclin B1 revealed that the mRNA is absent in the mutant embryo by gastrulation. We found that the earliest visible mutant phenotype was a darkening of the head caused …

Evolutionary Convergence Of The Caffeine Biosynthetic Pathway In Chocolate Followed Duplication Of A Constrained Ancestral Enzyme, Andrew J. O'Donnell

Masters Theses

Caffeine biosynthesis is widely distributed in flowering plants and requires three consecutive methylation steps of xanthine alkaloids. Genes that have previously been reported to participate in the multi-step pathway in Coffea sp. (coffee) and Camellia sinensis (tea) encode members of the SABATH family of methyltransferases. Two genes highly expressed in fruits of Theobroma cacao (cacao) are orthologous to the caffeine genes in tea and appear to have diversified following gene duplication. Biochemical characterization of the enzymes (XMTs) encoded by these genes strongly suggest an unprecedented major pathway to theobromine, a precursor to caffeine. These findings imply that caffeine biosynthesis evolved …

Regulation Of Glial Cell Line-Derived Neurotrophic Factor (Gdnf) Production In Voluntary And Involuntary Muscle, John-Mary Vianney

Dissertations

Glia cell line-derived neurotrophic factor (GDNF) is a survival factor for subpopulations of neurons, including somatic and autonomic motor neurons. These neurons depend, in part, on GDNF that is synthesized and secreted by their target tissues. It has been shown that a number of tissues in the periphery express GDNF and these target tissues differ in their composition, function, and in the case of different muscle cell types, their contractile characteristics. Whether the processes regulating GDNF production in these different tissues is similar or different is poorly understood. The broad goal of this study is to examine factors that normally …

A Gravity Investigation Of The Tobacco Root Batholith Southwest Montana, Stephen Charles Tatum

Masters Theses

The objective of this research was to delineate and profile the Late Cretaceous Tobacco Root batholith in southwestern Montana through the application of the gravitational method. This survey was accomplished by obtaining 232 gravity measurements in four profiles across the batholith. After correcting for known variations in the gravity field, a Bouguer anomaly map of the batholith and surrounding host rocks was created. Four residual gravity profiles and map, created by subtracting the regional gravity from the Bouguer anomaly, reveal a maximum negative gravity anomaly in the center portion of the batholith which is parallel to the trend of the …

Failing To Replicate: Hypothesis Testing As A Crucial Key To Make Direct Replications More Credible And Predictable, Pedro Fernando Mateu Bullón

Dissertations

Theory cannot be fully validated unless the original results have been replicated, resulting in conclusion consistency. Replications are the strongest source to verify research findings and knowledge claims. Sciences such as medicine, chemistry, physics, genetics, and biology, are considered successful because their knowledge claims are buttressed by a large set of replications of original studies. Unfortunately in the social sciences many attempts to replicate fail and thus there is a continuing need for replication studies to confirm facts, expand knowledge to gain new understanding, and verify hypotheses. Two plausible explanations for the failure to replicate in the social sciences could …

Innovative Governance And Natural Resource Management In Kenya: Procedural And Substantive Outcomes Of Civil Society Participation, Jane Omudho Okwako

Dissertations

Kenya’s environmental sector is embracing co-management to address major threats to wildlife. In the past two decades, the Municipal-Community-Private Sector Partnership (MCPP) model evolved to address the threats. This dissertation seeks to explain variations in partnership outcomes. It evaluates whether the model as introduced empowers communities to be conservation stewards.

This study hypothesized the impact of five variables. These are decentralization of power, elite support, capacity of community organizations, partnership formalization, and resources expended. The findings confirm that three variables are indispensable and two minimally influence empowerment. More decentralized management structures are enabling and supportive of empowerment. However, empowerment is …

Family Planning, Pregnancy, And Birth In Guatemala: Maya Women And Modern Healthcare, Kelsay Payton

Honors Theses

Within Maya populations in rural Guatemala, childbearing is considered a sacred and spiritual experience, in addition to a physical one. This country is home to some of the highest rates of maternal and infant mortality in Latin America, along with the largest disparities in health care, primarily between the indigenous Maya and ladino women (Schooley 2009). The use of biomedical reproductive services remains surprisingly low among the Maya, while it is continually rising in the ladino populations. To increase the overall reproductive health care in these indigenous communities, attention must be given to the understanding of Maya’s beliefs, therefore enabling …

Outline Plan For A Student-Involved Fitness Assessment Program At Western Michigan University, Joanna Gangwisch

Honors Theses

This research was aimed at developing a fitness assessment program that would provide undergraduate exercise science students at Western Michigan University (WMU) with hands on experience that would successfully prepare them for their internships and future careers as well as benefiting the campus community at large. Data for this research was obtained by examining the student recreation center websites and sending out an email/phone questionnaire to WMU’s 10 peer institutions. Direct comparisons were made and evaluated. On the basis of the results of this research, it can be concluded that WMU’s exercise science department and student recreation center together could …

Fueling Performance: Determining The Efficacy Of Low-Carbohydrate Diet Models For Athletic Populations, Chelsea Jackle

Honors Theses

Advocates of low-carbohydrate (LC) diets assert that these diets help lessen dependence on sugar while aiding in the metabolism of fat tissue. Additionally, it is believed that lipolysis helps dieters feel more energetic, lose weight, and improve physical performance. For these reasons, many athletes and active people are drawn to diets such as the Atkins Diet, the South Beach Diet, the Paleo Diet, and the Zone Diet. According to current evidence, excessive weight loss, ketosis, reduced glycogen availability, dehydration, and increased stress hormone levels make LC diets that severely limit carbohydrates disadvantageous for athletes. Although LC diets are widely followed, …

Technology And Nutrition: Interactive Strategies For Children To Learn Nutrition, Nicole Bogden

Honors Theses

This manuscript aims to describe nutritional requirements and needs for children and how technology is being used to improve nutritional knowledge among them. Several technologies and applications are explored, and recommendations are given for ways to improve. Four interactive nutrition games for children are examined: two computer games and two mobile applications. Through revision of performance, nutrition content, attraction, design and attention capabilities, HealthyHeroes was chosen to be the best game for children to learn and have fun with the subject of nutrition.