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Life Sciences

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Utah State University

Scanning Microscopy

Journal

1988

Light microscopy

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Visualization And Analysis Techniques For Three Dimensional Information Acquired By Confocal Microscopy, G. J. Brakenhoff, H. T. M. Van Der Voort, M. W. Baarslag, B. Mans, J. L. Oud, R. Zwart, R. Van Driel Nov 1988

Visualization And Analysis Techniques For Three Dimensional Information Acquired By Confocal Microscopy, G. J. Brakenhoff, H. T. M. Van Der Voort, M. W. Baarslag, B. Mans, J. L. Oud, R. Zwart, R. Van Driel

Scanning Microscopy

Confocal Scanning Laser Microscopy (CSLM) is particularly well suited for the acquisition of 3-dimensional data of microscopic objects. In the CSLM a specific volume in the object is sampled during the imaging process and the result is stored in a digital computer as a three-dimensional memory array. Optimal use of these data requires both the development of effective visual representations as well as analysis methods. In addition to the well known stereoscopic representation method a number of alternatives for various purposes are presented. When rendering in terms of solid-looking or semitransparent objects is required, an algorithm based on a simulated …


Structure And Function Of Connective Tissue In Cardiac Muscle: Collagen Types I And Iii In Endomysial Struts And Pericellular Fibers, Thomas F. Robinson, Leona Cohen-Gould, Stephen M. Factor, Mahboubeh Eghbali, Olga O. Blumenfeld Feb 1988

Structure And Function Of Connective Tissue In Cardiac Muscle: Collagen Types I And Iii In Endomysial Struts And Pericellular Fibers, Thomas F. Robinson, Leona Cohen-Gould, Stephen M. Factor, Mahboubeh Eghbali, Olga O. Blumenfeld

Scanning Microscopy

Heart myocytes and capillaries are enmeshed in a complex array of connective tissue structures arranged in several levels of organization: epimysium, the sheath of connective tissue that surrounds muscles; perimysium, which is associated with groups of cells; and endomysium, which surrounds and interconnects individual cells. The present paper is a review of work in this field with an emphasis on new, unpublished findings, including composition of endomysial fibers and disposition of newly described perimysial fibers. The role of scanning electron microscopy in the development of current understanding is also outlined. Biaxially arranged epimysial fibers form a sheath around papillary muscles …