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Regulatory Mechanisms In Borrelia Burgdorferi-Induced Arthritis, Emily Siebers Hansen
Regulatory Mechanisms In Borrelia Burgdorferi-Induced Arthritis, Emily Siebers Hansen
Theses and Dissertations
Lyme arthritis is a common symptom of Lyme borreliosis that involves inflammation of the synovial joints. Elucidating the immune events involved in Lyme arthritis is complicated by the fact that not all individuals infected with B. burgdorferi develop arthritis. Additionally, Lyme arthritis manifests in different severities between affected individuals. It is known that an inflammatory response is initiated by B. burgdorferi infection and that inflammatory T cells contribute to the development of arthritis. However, the anti-inflammatory mechanisms that regulate the pathogenic T cells’ response are not entirely understood. Here, the hypothesis that a dysregulated immune response results in an excessive …
Determining The Impacts Of Environmental Contaminants To Zebra Mussels Using Genetic Biomarkers, Nicklaus James Neureuther
Determining The Impacts Of Environmental Contaminants To Zebra Mussels Using Genetic Biomarkers, Nicklaus James Neureuther
Theses and Dissertations
ABSTRACT
DETERMINING THE IMPACTS OF ENVIRONMENTAL CONTAMINANTS TO ZEBRA MUSSELS USING GENETIC BIOMARKERS
by
Nicklaus James Neureuther
The University of Wisconsin-Milwaukee, 2016
Under the Supervision of Professor Rebecca Klaper, PhD
Persistent legacy contaminants and emerging chemicals of concern continue to be a threat to the function and health in the Great Lakes Areas of Concern (AOCs). While chemical monitoring programs traditionally sample water and sediment, these studies can only provide information of the type and level of contamination within an (AOC). This being said, information on the biological impacts to the biota are needed to measure impairments of chemical exposure, …
Symmetry And Reconstruction Of Particle Structure From Random Angle Diffraction Patterns, Sandi Wibowo
Symmetry And Reconstruction Of Particle Structure From Random Angle Diffraction Patterns, Sandi Wibowo
Theses and Dissertations
The problem of determining the structure of a biomolecule, when all the evidence from experiment consists of individual diffraction patterns from random particle orientations, is the central theoretical problem with an XFEL. One of the methods proposed is a calculation over all measured diffraction patterns of the average angular correlations between pairs of points on the diffraction patterns. It is possible to construct from these a matrix B characterized by angular momentum quantum number l, and whose elements are characterized by radii q and q’ of the resolution shells. If matrix B is considered as dot product of vectors, which …
Molecular And Functional Analysis Of The Pixb Gene In Xenorhabdus Nematophila, John Lucas
Molecular And Functional Analysis Of The Pixb Gene In Xenorhabdus Nematophila, John Lucas
Theses and Dissertations
Xenorhabdus nematophila and the entomopathogenic nematode Steinernema carpocapsae form a mutualistic relationship facilitating the infection, death and consumption of an insect host. The infective juvenile (IJ) form of S. carpocapsae invades the insect host through natural openings and proceeds to the hemocoel where exposure to hemolymph stimulates the release of X. nematophila from the anterior vesicle. Excreted X. nematophila releases immunosuppressive compounds and insect toxins into the insect hemolymph that facilitates death of the host. As X. nematophila reaches high cell density it secretes exoenzymes that degrade insect tissues and produces antibiotics that reduce microbial competition. S. carpocapsae utilizes the …
Apoaequorin Differentially Modulates Fear Conditioning In Adult And Aged Rats, Vanessa Ehlers
Apoaequorin Differentially Modulates Fear Conditioning In Adult And Aged Rats, Vanessa Ehlers
Theses and Dissertations
Normal aging is associated with a number of changes in behavioral and cellular function, and is often linked to increased susceptibility to cognitive impairment. The hippocampus has been widely implicated in learning and memory, and many forms of learning that are hippocampus-dependent (e.g. trace fear conditioning) are impaired in aged animals. A proposed contributor to aging-related cognitive impairment is aging-related calcium (Ca2+) dysregulation. This dysregulation is thought to result from changes in specific Ca2+-regulatory mechanisms, including abnormal Ca2+ ion channel activity or expression, as well as reduced Ca2+-binding protein (CaBP) expression, which is associated with cognitive and synaptic impairment. Previous …
Late Paleo-Indian Period Lithic Economies, Mobility, And Group Organization In Wisconsin, Ethan Adam Epstein
Late Paleo-Indian Period Lithic Economies, Mobility, And Group Organization In Wisconsin, Ethan Adam Epstein
Theses and Dissertations
The following dissertation focuses upon the organization of Pleistocene / Holocene period lithic technology in Wisconsin circa 10,000 – 10,500 years before present. Lithic debitage and flaked stone tools from the Plainview/Agate Basin components of the Heyrman I site (47DR381), the Dalles site (47IA374), and the Kelly North Tract site at Carcajou Point (47JE02) comprise the data set. These Wisconsin sites are located within a post glacial Great Lakes dune environment, an inland drainage/riverine environment, and an inland wetland/lacustrine environment. An assemblage approach is used to examine the structure of each site’s lithic economy. This broad approach to lithic organization …
The Effects Of Exercise Pattern And Intensity On The Expression Of Flk-1 And Flt-1 Receptors In The Hippocampus And Cerebellum, Morgan E. Stevenson
The Effects Of Exercise Pattern And Intensity On The Expression Of Flk-1 And Flt-1 Receptors In The Hippocampus And Cerebellum, Morgan E. Stevenson
Theses and Dissertations
Aerobic exercise benefits the body and brain. In the brain, these benefits include neuroprotection and improved cognition. These exercise-induced changes are attributed in part to angiogenesis: the growth of new capillaries from preexisting vessels. One critical factor involved in the regulation of angiogenesis is VEGF and its receptors Flk-1 and Flt-1. Although exercise is generally found to be beneficial, there are wide variations in the exercise regimens used across experiments. This study standardized some of these variations. In this study, rats were either assigned to a voluntary wheel running or a forced wheel running exercise condition. Within each condition, animals …
Ultrastructural Changes During Pollen Wall Development And Germination In Arabidopsis Thalaiana, Katrina Olsen
Ultrastructural Changes During Pollen Wall Development And Germination In Arabidopsis Thalaiana, Katrina Olsen
Theses and Dissertations
The Arabidopsis thaliana meiotic mutant 6491 has been identified as displaying temperature sensitive male reduced- fertility. It has been determined that callose wall formation is defective, both in temporal and structural areas. There is irregular rippling in the plasma membrane and aberrant formation of the exine portion of the pollen wall. A developmental study using brightfield, epifluorescence, and transmission electron microscopy of the early stages of wall formation in 6491 has been completed, along with a similar study of Arabidopsis thaliana (L.) heynh, ecotype Wassilewskija (WS). Due to the temperature-sensitive nature of the mutant line, a further study of both …
Characterization And Use Of Folate Receptor Isoforms For Targeting Of Epithelial And Myeloid Cells, Sreya Biswas
Characterization And Use Of Folate Receptor Isoforms For Targeting Of Epithelial And Myeloid Cells, Sreya Biswas
Theses and Dissertations
ABSTRACT
CHARACTERIZATION AND USE OF FOLATE RECEPTOR ISOFORMS FOR TARGETING OF EPITHELIAL AND MYELOID CELLS
by
Sreya Biswas
The University of Wisconsin-Milwaukee, 2016
Under the Supervision of Professor Douglas A. Steeber
Folate receptor (FR) is a GPI-anchored glycoprotein with high binding affinity for folic acid. FR has two membrane-associated isoforms, α and β, that are overexpressed on epithelial and myeloid tumors, respectively. Normal cells may also exhibit FR expression at very low levels but interestingly, FR-α on normal cells is restricted to the apical surface i.e., away from the blood stream. This differential expression and orientation of the FR-α isoform …
Hashtagging Your Health: Using Psychosocial Variables And Social Media Use To Understand Impression Management And Exercise Behaviors In Women, Caitlyn Hauff
Theses and Dissertations
Our society has become heavily reliant on social media, especially in the health and exercise domain. Social and environmental factors impact females’ body image perceptions and create body image disturbances, yet little research is dedicated to the exploration of how social media, and social comparisons through social media exposure, impact exercise behaviors and body image perceptions in females. Considering Perloff's (2014) theoretical model, the current study explored how the interaction between individual psychosocial variables and social media use predict exercise behaviors and engagement in impression management in women. Using a mixed methodological approach, the specific aims of this study were …
Stage-Specific Predictive Models For Cancer Survivability, Elham Sagheb Hossein Pour
Stage-Specific Predictive Models For Cancer Survivability, Elham Sagheb Hossein Pour
Theses and Dissertations
Survivability of cancer strongly depends on the stage of cancer. In most previous works, machine learning survivability prediction models for a particular cancer, were trained and evaluated together on all stages of the cancer. In this work, we trained and evaluated survivability prediction models for five major cancers, together on all stages and separately for every stage. We named these models joint and stage-specific models respectively. The obtained results for the cancers which we investigated reveal that, the best model to predict the survivability of the cancer for one specific stage is the model which is specifically built for that …
Point Of Use Biosand Filters Of The Rural Dominican Republic, Kurtis Quamme
Point Of Use Biosand Filters Of The Rural Dominican Republic, Kurtis Quamme
Theses and Dissertations
The point of use biosand filter (BSF) is used globally as a drinking water treatment solution. In this research, point of use BSFs were inoculated with active biosand from the Linnwood Drinking Water Treatment plant slow sand filter beds (Milwaukee, Wisconsin) and with sands collected from point of use filters operating in the Dominican Republic. These filters were maintained with varying source waters (surface water, groundwater, or tap water to simulate chlorination encountered in the field). The microbial community of filters with varied influents and biosand inoculum were analyzed quantitatively by sequencing and qPCR. Filter efficacy and microbial community were …
Neural Circuitry Underlying The Intrusion Of Task-Irrelevant Threat Into Working Memory In Anxiety, Daniel Stout
Neural Circuitry Underlying The Intrusion Of Task-Irrelevant Threat Into Working Memory In Anxiety, Daniel Stout
Theses and Dissertations
Dispositional anxiety is an important risk factor for the development of anxiety and other psychological disorders. Symptoms commonly expressed by highly anxious individuals include intrusive memories, uncertainty, and worry — all occurring in the absence of immediate threat. This raises the possibility that anxious individuals have difficulty governing threat’s access to working memory, the mental workspace where goal-related information is actively retained for guiding on-going behavior. Using functional magnetic resonance imaging (fMRI) while 81 subjects completed a well-validated working memory task, I show that threat-related and neutral distracters unnecessarily gain access to working memory, as evidenced by increased neural activity …
The Benthic Feeding Ecology Of Round Goby Fry, Dylan Samuel Olson
The Benthic Feeding Ecology Of Round Goby Fry, Dylan Samuel Olson
Theses and Dissertations
Larval and juvenile stage events play a dominant role in regulating the ultimate recruitment strength of fish populations. As such, the feeding ecology of early life stages are useful for interpreting the proximate causes of recruitment variability. This study provides the first targeted study of the early juvenile (“fry”) diet of the round goby (Neogobius melanostomus, Pallas 1814), a prominent Great Lakes invasive fish. Previous accounts of the diets of round goby fry in the Great Lakes have been based upon by-catch from nocturnal, pelagic studies. Alternatively, we sampled diurnally (day) in diverse benthic environments including open lake, embayment, and …
Development Of An Automated Detection System For Nitrite In Aquatic Environments, Tim Schierenbeck
Development Of An Automated Detection System For Nitrite In Aquatic Environments, Tim Schierenbeck
Theses and Dissertations
The main objective of the project is to develop an automated nitrite sensor for use in aquatic environments, and more specifically for use in recirculating aquaculture systems (RAS), where monitoring can help sustain a controlled environment, protect against nitrite intoxication, and promote fish health. Detecting nitrite manually with semi-quantitative colorimetric test kits, although inexpensive and simple, is prone to inter-user variability and poor sensitivity. An automated nitrite sensor has potential to provide higher resolution measurements at both concentration and time scales and can serve as a research tool for the study of filtration systems essential in maintaining a healthy RAS …
Assessment Of Benthic Habitat Quality In Lower Green Bay, Lake Michigan With Special Regard To Potential Hexagenia Recolonization, Christopher Michael Groff
Assessment Of Benthic Habitat Quality In Lower Green Bay, Lake Michigan With Special Regard To Potential Hexagenia Recolonization, Christopher Michael Groff
Theses and Dissertations
With environmental remediation in the Great Lakes, Hexagenia have recovered or are recovering in systems from which they were once extirpated. An active Hexagenia recovery does not appear to be taking place in lower Green Bay. This study first examines the highly fluidized nature of lower Green Bay sediment as a possible cause for their lack of recovery due to nymphs’ potential inability to construct and maintain burrows essential to the completion of their life cycles. Hexagenia bilineata nymphs collected from the Upper Mississippi River were distributed into oxygenated aquaria containing substrates from lower Green Bay or the Upper Mississippi …
Discovery And Characterization Of A New Group Of Is10 Insertion Sequences, Rachel Marie Kinzelman
Discovery And Characterization Of A New Group Of Is10 Insertion Sequences, Rachel Marie Kinzelman
Theses and Dissertations
Insertion sequences (ISs) are small mobile genetic elements that can have significant impact on the genotype and phenotype of a host organism. Previous work in this laboratory revealed an insertion sequence that disrupted the luxA gene in Vibrio harveyi strain BCB451, knocking out light production. Phylogenetic analysis of this insertion sequence, dubbed IS451, reveals that it is in the IS10 family, but represents a novel variant that is only 79% identical to other known IS10 sequences. Twelve copies of IS451 were isolated from a genomic library and sequenced, and were found to be essentially identical, but located in dispersed chromosomal …
The Role Of Proteome In Cellular Zn2+ Trafficking And In The Ability Of The Fluorescent Zinc Sensors To Image Intracellular Zn2+, Mohammad Rezaul Karim
The Role Of Proteome In Cellular Zn2+ Trafficking And In The Ability Of The Fluorescent Zinc Sensors To Image Intracellular Zn2+, Mohammad Rezaul Karim
Theses and Dissertations
Zinc is an essential biological trace metal used in as many as 3000 Zn-proteins, about 10% of the eukaryotic proteome, as either a structural constituent or a catalytic cofactor. These proteins include the zinc fingers, the most prevalent transcription factors that bind a wide range of gene promoters and thus regulate gene expression. A eukaryotic cell contains several hundred micromolar of Zn2+- almost all of it is bound to specific Zn-proteins. Recently, Zn2+ has been reported to serve as a regulatory signal and a neurotransmitter, suggesting that there also exists a dynamic Zn2+ pool in cells. These findings led to …
Structural And Functional Characterization Of Acetoacetate Decarboxylase-Like Enzymes, Lisa Mueller
Structural And Functional Characterization Of Acetoacetate Decarboxylase-Like Enzymes, Lisa Mueller
Theses and Dissertations
The acetatoacetate decarboxylase-like superfamily (ADCSF) is a largely unexplored group of enzymes that may be a potential source of new biocatalysts. Bioinformatic analysis has grouped these approximately 2000 enzymes into seven different families based on comparison of predicted active site residues. To date, only the prototypical ADCs (Family I) that catalyze the decarboxylation of acetoacetate have been studied. Analysis of gene context suggests that Family V contains predominantly enzymes predicted to be involved in secondary metabolism. On average, these share about 20% sequence identity to the true ADCs. To learn more about the diversity of chemistries performed by members of …
Spectral Domain-Optical Coherence Tomography For The Assessment Of Cerebrovascular Plasticity, Jacob James Michael Kay
Spectral Domain-Optical Coherence Tomography For The Assessment Of Cerebrovascular Plasticity, Jacob James Michael Kay
Theses and Dissertations
Vascular pathologies represent the leading causes of mortality worldwide, accounting for 31% of all deaths in 2012. Cerebral hypoxia is a condition that often manifests as a result of these medical conditions. Remarkably, the nervous system has evolved mechanisms to compensate for oxygen deprivation. The dilation of existing vessels and the growth of new blood vessels are two prominent physiological responses to hypoxia, both of which play a critical role in maintaining cerebral homeostasis. More recently, exercise has been shown to induce a mild state of hypoxia in the brain, leading to several robust morphological changes within the cerebrovascular system …
Neural Plasticity Of Extinction: Relations With Anxiety And Extinction Retention, Emily Louise Belleau
Neural Plasticity Of Extinction: Relations With Anxiety And Extinction Retention, Emily Louise Belleau
Theses and Dissertations
ABSTRACT
NEURAL PLASTICITY OF EXTINCTION LEARNING: RELATIONS WITH ANXIETY AND EXTINCTION RETENTION
by
Emily L. Belleau
The University of Wisconsin-Milwaukee, 2016
Under the Supervision of Associate Professor Christine Larson
Anxiety is a significant public health problem characterized by substantial psychological, physical, and economic burden. A key feature of anxiety is the inability to regulate fear. Aberrant extinction of conditioned fear is one prominent model of the etiology of anxiety disorders. Previous studies have shown that the neural circuitry underlying anxiety pathology overlaps with that mediating fear extinction learning. Recently, more precise pathways supporting the expression (CMA-aMCC) and inhibition (BLA-vmPFC) of …
An Examination Of Light Intensity Physical Activity And Health In Older Adults, Whitney A. Welch
An Examination Of Light Intensity Physical Activity And Health In Older Adults, Whitney A. Welch
Theses and Dissertations
Research has begun to quickly emerge on the potential benefit of light intensity physical activity (LPA) to the health of adults. Little is known about LPA, and much of the current LPA research stems from sedentary behavior research. The purpose of this dissertation was to more fully understand, describe, and characterize potential health benefits of LPA by determining the prevalence, patterns, and health benefits of light intensity physical activity in older adults. Three individual studies were completed to address each portion of this purpose. Study 1: Light Intensity Physical Activity and Health in Adults: A Systematic Review. The purpose of …
Brain Mechanism For Enhanced Hand Function With Remote Sensory Stimulation, Kishor Lakshminarayanan
Brain Mechanism For Enhanced Hand Function With Remote Sensory Stimulation, Kishor Lakshminarayanan
Theses and Dissertations
The neurological bases for remote vibration enhanced sensory feedback and motor function are yet poorly understood. The purpose of this dissertation was to identify and examine the effect of vibration on finger tactile sensation in healthy adults and how imperceptible random vibration applied to the wrist changes cortical activity for fingertip sensation and precision grip. In a series of studies on healthy adults, white-noise vibration was applied to one of four locations (dorsum hand by the second knuckle, thenar and hypothenar areas, and volar wrist) at one of four intensities (zero, 60%, 80%, and 120% of the sensory threshold for …
Lateral Replacement Of The Lux Operon In A Vibrio Isolated From The Intestine Of A Coral Reef Fish, Melissa Lee Whyte
Lateral Replacement Of The Lux Operon In A Vibrio Isolated From The Intestine Of A Coral Reef Fish, Melissa Lee Whyte
Theses and Dissertations
In a screening of bioluminescent bacteria isolated from the intestines of coral reef fish, two strains (designated D6 and M1) were identified that have a luxA gene sequence significantly different from those of other Vibrio species. Phylogenetic analysis of several housekeeping genes, as well as toxR, shows that D6 and M1 branch within a bioluminescent clade (designated the “D1 group,” isolated at the same time and place as D6 and M1) that is a close sister group to Vibrio harveyi. However, whereas the luxA genes of the D1 group are >98% identical to V. harveyi luxA, the luxA genes of …
Mhealth Technology: Towards A New Persuasive Mobile Application For Caregivers That Addresses Motivation And Usability, Suboh M. Alkhushayni
Mhealth Technology: Towards A New Persuasive Mobile Application For Caregivers That Addresses Motivation And Usability, Suboh M. Alkhushayni
Theses and Dissertations
With the increasing use of mobile technologies and smartphones, new methods of promoting personal health have been developed. For example, there is now software for recording and tracking one's exercise activity or blood pressure. Even though there are already many of these services, the mobile health field still presents many opportunities for new research.
One apparent area of need would be software to support the efforts of caregivers for the elderly, especially those who suffer from multiple chronic conditions, such as cognitive impairment, chronic heart failure or diabetes. Very few mobile applications (apps) have been created that target caregivers of …
The Mechanisms Underlying Cocaine-Induced Overexpression Of Basic Fibroblast Growth Factor (Bfgf, Fgf2), An Effect Reversed By Extinction, Madalyn Hafenbreidel
The Mechanisms Underlying Cocaine-Induced Overexpression Of Basic Fibroblast Growth Factor (Bfgf, Fgf2), An Effect Reversed By Extinction, Madalyn Hafenbreidel
Theses and Dissertations
Drug addiction is characterized by compulsive drug use and chronic relapse despite negative consequences. Drug-induced structural and functional changes in the brain are thought to underlie these characteristics. One mechanism that may mediate these characteristics are growth factors, such as basic fibroblast growth factor (bFGF or FGF2), as they are necessary for cellular growth, survival, differentiation, and have roles in memory, mood, and anxiety disorders. bFGF mRNA and protein expression is increased following stimulant administration and is necessary for stimulant-induced changes in dendrites and behavioral sensitization. Moreover, addiction is maintained by cues associated with the drug, as they can can …
Development Of Cellular High Throughput Assays To Determine The Electrophysiological Profile Of Gaba(A) Receptor Modulators For Neurology And Immunology, Nina Yina Yuan
Theses and Dissertations
Gamma (γ) -aminobutyric acid (GABA) is the major inhibitory neurotransmitter found in
the mammalian central nervous system. Its effect stems from its ability to cause the opening of ion channels which causes an influx of negatively charged chloride ions or an efflux of positively charged potassium ions. This hyperpolarization of the neuron lowers the threshold for neuronal firing. This has an overall inhibitory effect on neurotransmission, decreasing the excitability of the neuron and diminishing the likelihood of a successful action potential occurring. There are two classes of GABA receptor: ligand-gated GABAA receptor (GABAAR) and metabotropic GABAB receptor (GABABR). The GABAAR …
The Function Of Renalase, Brett Allen Beaupre
The Function Of Renalase, Brett Allen Beaupre
Theses and Dissertations
Renalase was originally reported to be an enzyme secreted into the blood by the kidney to lower blood pressure and slow heart rate. Despite multiple reports claiming to confirm this activity in vivo there has been considerable discord in regards to the reaction catalyzed by renalase. The structural topology of renalase resembles that of known flavoprotein oxidases, monooxygenases and demethylases, but the conserved active site residues are unique to renalase. It has been reported that the catalytic function of renalase is to oxidize circulating catecholamines, however in vitro studies have failed to demonstrate a catalytic activity in the presence of …
Identifying Gait Deficits In Stroke Patients Using Inertial Sensors, Lauren Benson
Identifying Gait Deficits In Stroke Patients Using Inertial Sensors, Lauren Benson
Theses and Dissertations
Falls remain a significant problem for stroke patients. Tripping, the main cause of falls, occurs when there is insufficient clearance between the foot and ground. Based on an individual’s gait deficits, different joint angles and coordination patterns are necessary to achieve adequate foot clearance during walking. However, gait deficits are typically only quantified in a research or clinical setting, and it would be helpful to use wearable devices – such as accelerometers – to quantify gait disorders in real-world situations. Therefore, the objective of this project was to understand gait characteristics that influence the risk of tripping, and to detect …
The Effects Of Military Style Ruck Marching On Lower Extremity Loading, Daniel N. Poel
The Effects Of Military Style Ruck Marching On Lower Extremity Loading, Daniel N. Poel
Theses and Dissertations
Load carriage while performing prolonged marches may play a role in military overuse injuries. It is known that both external load carriage and muscular fatigue can contribute to increases in ground reaction forces and loading rate and play a role in stress injuries. The purpose of this study is to determine whether or not a prolonged military style ruck march will cause changes in vertical ground reaction force and loading rate. 15 healthy members of the Army ROTC and Army National Guard performed vertical jumps, had ankle dorsiflexion, plantarflexion, inversion and eversion strength measured, and walked across a force plate …