Digital Commons Network^™

Identification Of Bile Salt Hydrolase Inhibitors, The Promising Alternative To Antibiotic Growth Promoters To Enhance Animal Production, Katie Rose Smith

Masters Theses

Currently there is a global trend to eliminate the use of antibiotic growth promoters (AGP) in animal production due to the risk of creating reservoirs of antibiotic resistant bacteria. Previous studies have shown that the ability of AGP to promote growth is highly correlated with a decrease in the activity of bile salt hydrolase (BSH), an enzyme produced by commensal bacteria and whose actions may compromise host lipid metabolism; thus, BSH inhibitors could be a novel AGP alternative. In this study, a recombinant bile salt hydrolase (rBSH) from Lactobacillus salivarius was produced in an E. coli expression system, purified, and …