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Regulation Of Yeast Phospholipid Biosynthesisinvolves Two Superimposed Mechanisms, John Lopes, B. P. Ashburner
Regulation Of Yeast Phospholipid Biosynthesisinvolves Two Superimposed Mechanisms, John Lopes, B. P. Ashburner
John Lopes
Transcription of phospholipid biosynthetic genes in the yeast Saccharomyces cerevisiae is maximally derepressed when cells are grown in the absence of inositol and repressed when the cells are grown in its presence. We have previously suggested that this response to inositol may be dictated by regulating transcription of the cognate activator gene, INO2. However, it was also known that cells which harbor a mutant opi1 allele express constitutively derepressed levels of target genes (INO1 and CHO1), implicating the OPI1 negative regulatory gene in the response to inositol. These observations suggested that the response to inositol may involve both regulation of …
Autoregulated Expression Of The Yeast Ino2 And Ino4 Helix-Loop-Helix Genes Effects Cooperative Regulation On Their Target Genes, John Lopes, B. P. Ashburner
Autoregulated Expression Of The Yeast Ino2 And Ino4 Helix-Loop-Helix Genes Effects Cooperative Regulation On Their Target Genes, John Lopes, B. P. Ashburner
John Lopes
In the yeast Saccharomyces cerevisiae, the phospholipid biosynthetic genes are highly regulated at the transcriptional level in response to the phospholipid precursors inositol and choline. In the absence of inositol and choline (derepressing), the products of the INO2 and INO4 genes form a heteromeric complex which binds to a 10-bp element, upstream activation sequence INO (UASINO), in the promoters of the phospholipid biosynthetic genes to activate their transcription. In the presence of inositol and choline (repressing), the product of the OPI1 gene represses transcription dictated by the UASINO element. Curiously, we identified a UASINO-like element in the promoters of both …
Zinc Metabolism In The Streptozotocin (Stz)-Diabetes, Aizhong Fu
Zinc Metabolism In The Streptozotocin (Stz)-Diabetes, Aizhong Fu
Doctoral Dissertations 1896 - February 2014
Hyperzincuria in diabetics has been regarded as the culprit depleting body zinc stores. Studies were designed to assess rates of 65Zn absorption and retention as a possible compensation mechanism; to assess zinc concentrations and distribution among body compartments, and to assess the kinetics of 65Zn metabolism in STZ-diabetic rats. The rates of 65Zn absorption and retention were not significantly different between STZ-diabetic and control rats. However, STZ-diabetic rats had significantly higher rates of 65Zn absorption (16.88%) and retention (34.36%) when they were "Post-fasted" than when they were "Prior-fasted" (9.04% and 18.68% respectively). These differences were also present in control rats …
Autoregulated Expression Of The Yeast Ino2 And Ino4 Helix-Loop-Helix Genes Effects Cooperative Regulation On Their Target Genes, John Lopes, B. Ashburner
Autoregulated Expression Of The Yeast Ino2 And Ino4 Helix-Loop-Helix Genes Effects Cooperative Regulation On Their Target Genes, John Lopes, B. Ashburner
Microbiology Department Faculty Publication Series
In the yeast Saccharomyces cerevisiae, the phospholipid biosynthetic genes are highly regulated at the transcriptional level in response to the phospholipid precursors inositol and choline. In the absence of inositol and choline (derepressing), the products of the INO2 and INO4 genes form a heteromeric complex which binds to a 10-bp element, upstream activation sequence INO (UASINO), in the promoters of the phospholipid biosynthetic genes to activate their transcription. In the presence of inositol and choline (repressing), the product of the OPI1 gene represses transcription dictated by the UASINO element. Curiously, we identified a UASINO-like element in the promoters of both …
Cranberry Tissue Testing, Joan Davenport, Et Al
Cranberry Tissue Testing, Joan Davenport, Et Al
Cranberry Station Fact Sheets
No abstract provided.
Weed Mapping As A Component Of Ipm In Cranberry Production, Mary Jane Else, Hilary A Sandler, Scott Schluter
Weed Mapping As A Component Of Ipm In Cranberry Production, Mary Jane Else, Hilary A Sandler, Scott Schluter
Cranberry Station Fact Sheets
No abstract provided.
Long Term Maintenance Of Presynaptic Function In The Absence Of Target Muscle Fibers, Elizabeth A. Connor, A. Duneavsky
Long Term Maintenance Of Presynaptic Function In The Absence Of Target Muscle Fibers, Elizabeth A. Connor, A. Duneavsky
Elizabeth A. Connor
Here we have investigated the role of the muscle fiber in the maintenance of presynaptic function at frog motor nerve terminals. Muscle fibers were selectively damaged and prevented from regenerating while leaving the motor innervation intact. Presynaptic activity of the resulting target-deprived nerve terminals was assayed using the fluorescent dye, FM1–43. FM1–43 stains nerve terminals in an activity- dependent fashion in that it incorporates into synaptic vesicles and can be released upon stimulation. We demonstrate that nerve terminals permanently deprived of target for 1 to 5 months maintain the ability to release and recycle synaptic vesicles in response to stimulation. …
Song Types, Repertories And Son Variability In A Population Of Chestnut-Sided Warblers, Bruce Byers
Song Types, Repertories And Son Variability In A Population Of Chestnut-Sided Warblers, Bruce Byers
Bruce Byers
No abstract provided.
Long-Term Maintenance Of Presynaptic Function In The Absence Of Target Muscle-Fibers, Anna Dunaevsky, Elizabeth A. Connor
Long-Term Maintenance Of Presynaptic Function In The Absence Of Target Muscle-Fibers, Anna Dunaevsky, Elizabeth A. Connor
Biology Department Faculty Publication Series
Here we have investigated the role of the muscle fiber in the maintenance of presynaptic function at frog motor nerve terminals. Muscle fibers were selectively damaged and prevented from regenerating while leaving the motor innervation intact. Presynaptic activity of the resulting target-deprived nerve terminals was assayed using the fluorescent dye, FM1–43. FM1–43 stains nerve terminals in an activity- dependent fashion in that it incorporates into synaptic vesicles and can be released upon stimulation. We demonstrate that nerve terminals permanently deprived of target for 1 to 5 months maintain the ability to release and recycle synaptic vesicles in response to stimulation. …