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Perturbation Of Brachypodium Distachyon Cellulose Synthase A4 Or 7 Results In Abnormal Cell Walls, Pubudu P. Handakumbura, Dominick A. Matos, Karen S. Osmont, Michael J. Harrington, Kyuyoung Heo, Kabindra Kafle, Seong H. Kim, Tobias I. Baskin, Samuel P. Hazen
Perturbation Of Brachypodium Distachyon Cellulose Synthase A4 Or 7 Results In Abnormal Cell Walls, Pubudu P. Handakumbura, Dominick A. Matos, Karen S. Osmont, Michael J. Harrington, Kyuyoung Heo, Kabindra Kafle, Seong H. Kim, Tobias I. Baskin, Samuel P. Hazen
Samuel P Hazen
Background: Cellulose is an integral component of the plant cell wall and accounts for approximately forty percent of total plant biomass but understanding its mechanism of synthesis remains elusive. CELLULOSE SYNTHASE A (CESA) proteins function as catalytic subunits of a rosette-shaped complex that synthesizes cellulose at the plasma membrane. Arabidopsis thaliana and rice (Oryza sativa) secondary wall CESA loss-of-function mutants have weak stems and irregular or thin cell walls. Results: Here, we identify candidates for secondary wall CESAs in Brachypodium distachyon as having similar amino acid sequence and expression to those characterized in A. thaliana, namely CESA4/7/8. To functionally characterize …