Digital Commons Network^™

Development And Ex Vivo Characterization Of Enteric Coated Chitosan Beads For Crohn’S Disease Management, Craig Mendonca

University Scholar Projects

Drug delivery is the process of transporting a drug to its site of action. It includes controlled delivery, where the drug is released at the tissue in a regulated manner. Additionally, it includes targeted delivery, where the drug is aimed at a specific tissue or region to avoid unwanted side effects (that would occur if the drug were distributed throughout the body). A cellulose acetate phthalate coated chitosan bead was developed as a formulation for controlled and targeted release of dexamethasone to the ilium and colon. This formulation was determined to be effective in delaying dexamethasone release until the formulation …

Wrack Lines Volume 18, Number 2, Fall/Winter 2018-19, Judy Benson, Robert Miller, Christine Woodside, Judy Preston

Wrack Lines

Articles focus on 30 years of environmental history in Long Island Sound, specifically Norwalk, New Haven and Niantic harbors and the trends of two iconic species -- lobsters and shad.

Pertinacity Volume 4, Issue 2, Courteney L. Benjamin

Pertinacity

Pertinacity is a biannual magazine that features stories and reports on recent Korey Stringer Institute's initiatives.

Confirming World-Wide Distribution Of An Agriculturally Important Lacewing, Chrysoperla Zastrowi Sillemi, Using Songs, Morphology, Mitochondrial Gene Sequencing, And Phylogenetic Reconstruction, Zoe Mandese

Honors Scholar Theses

The Chrysoperla carnea-group of green lacewings is a cryptic species complex. Species within the group are morphologically similar, yet isolated from one another via reproductive mating song. Chrysoperla zastrowi, a species within the carnea-group, is currently described with a distribution ranging from South Africa to the Middle East and India. However, recent collections of carnea-group lacewings from Guatemala and California were preliminarily identified as Chrysoperla zastrowi based upon similarities in their vibrational courtship songs. This analysis aims to place six specimens, collected by collaborators in Guatemala, Armenia, Iran, and California, into a pre-existing phylogeny of the …

Modeling And Analyzing An Optogenetic System For Photoactivatable Protein Dissociation, Anvin Thomas, James Schaff

Honors Scholar Theses

Computational modeling of cell-cell interactions can grant clues and can answer questions about an experiment, especially for observations about binding interactions and kinetics. This approach was used to investigate an interaction between a light-oxygen-voltage (LOV) domain and an engineered protein called Zdark (Zdk). The LOV domain is membrane-bound while Zdk is cytosolic. The LOV domain and Zdk bind strongly in dark (K_d 26.2 nM), and weakly upon exposure to blue light (K_d > 4 μM). Total internal reflection fluorescence (TIRF) images are acquired of Zdk, the fluorescent species bound to a mCherry tag, and the loss of fluorescence is …

Juvenile Amphibian Response To Oak And Maple Leaf Litter, Benjamin Breslau

Honors Scholar Theses

The composition of tree species within New England forests has changed significantly in recent decades, with an increase in maple (Acer spp.) abundance and a decrease in oak (Quercus spp.) abundance. Changing forest structure results in changing leaf litter composition of the forest floor, which influences the ground-dwelling amphibians that live in the litter. To better understand how changes to forest composition alters amphibian habitat quality, we recorded the growth and survival of 27 juvenile wood frogs (Lithobates sylvaticus or Rana sylvatica) and 27 juvenile American toads (Anaxyrus americanus) in response to leaf litter …

Identification Of Genes Conferring Acid Resistance In Vibrio Parahaemolyticus, Erin Gibbons

Honors Scholar Theses

Vibrio parahaemolyticus has the capability to be acid resistant due to known and unknown mechanisms. The goal of this project was to determine possible genes involved in conferring acid resistance of this bacterium. The vp1277 gene (designated as HA) and its downstream gene vp1999 were investigated for their role in acid resistance. Another potential gene that could contribute to acid resistance is vp0820 because of its similarity to the ToxR gene in Vibrio cholera. All three of these knockout strains and the wild type were grown in neutral LB and then tested with acidic LB’s of pH 4.5, 5, and …

Effect Of Silk-Based Hydrogel Topography On Intestinal Epithelial Cell Morphology And Wound Healing In Vitro, Marisa E. Boch

University Scholar Projects

Recent advances in the field of biomaterials have suggested that cells cultured on substrates resembling the native tissue mechanical properties, matrix and growth factor composition, and topography can adopt phenotypes that more closely resemble the in vivo tissue compared to cells cultured on non-mimetic constructs. Understanding the effect of culture substrate on in vitro tissue formation is important for bioengineering applications that include mechanistic studies of healthy tissue function and development of disease models. In this work, Caco-2 adenocarcinoma cells were seeded on flat and crypt-like topographies of 3D-printed cytocompatible hydrogels derived from silk fibroin protein. Silk hydrogels were selected …

Pyruvate Kinase Isoform M2 Influences Autophagy And Related Processes In Hepatocellular Carcinoma Cells, Matthew Lin

University Scholar Projects

Hepatocellular carcinoma (HCC) is the most common form of liver cancer that affects ~14 million people in the world. Like all cancers, HCC is a disease that arises from unstinted cellular growth initiated by genetic alterations, metabolic changes, and dysregulation in key cellular pathways. Of interest is the relationship between metabolism and cell proliferation/degradation for therapeutic targeting. Pyruvate kinase M2 is a dimeric, glycolytically inactive isoform of the final enzyme involved in glycolysis, that is often upregulated in cancerous tissue. It is thought that the enzymatic function of PKM2 outside of glycolysis contributes to the biosynthesis of anabolic intermediates used …

Effect Of Silk-Based Hydrogel Topography On Intestinal Epithelial Cell Morphology And Wound Healing In Vitro, Marisa E. Boch

Honors Scholar Theses

Recent advances in the field of biomaterials have suggested that cells cultured on substrates resembling the native tissue mechanical properties, matrix and growth factor composition, and topography can adopt phenotypes that more closely resemble the in vivo tissue compared to cells cultured on non-mimetic constructs. Understanding the effect of culture substrate on in vitro tissue formation is important for bioengineering applications that include mechanistic studies of healthy tissue function and development of disease models. In this work, Caco-2 adenocarcinoma cells were seeded on flat and crypt-like topographies of 3D-printed cytocompatible hydrogels derived from silk fibroin protein. Silk hydrogels were selected …

Composition And Function Of The Bacterial Consortium Associated With The Accessory Nidamental Gland Of The Hawaiian Bobtail Squid, Jessica Bertenshaw

Honors Scholar Theses

The bacterial consortium associated with the accessory nidamental gland (ANG) of the Hawaiian bobtail squid, Euprymna Scolopes is posited to confer symbiotic benefits to the eggs of the squid when bacteria are transferred from the gland of the mother to the jelly coat, which surrounds the embryo in the egg. To characterize the composition of this community bacterial isolates from the egg jelly coat and from the ANG were identified using 16S sequencing. To elucidate the function of these bacteria, egg development experiments were performed to determine the effect of antibiotics on egg survival and the composition of the bacterial …

Sequencing And Analysis Of Centromere Protein B In Wallaby And The Rapid Evolution Of The Centromere, Alexander Tedeschi

Honors Scholar Theses

Using a combination of Sanger sequencing and RNA-seq data, this project aims to determine the nucleotide and amino acid sequence of Centromere Protein B (CENP-B), an important protein involved in the assembly of the kinetochore protein complex at the centromere, in several species of marsupials, specifically wallabies. Despite their recent evolutionary history, these species have been shown to have surprisingly divergent centromeric DNA sequences. Through comparative analysis of these sequences, this project, along with analysis of several other CENPs, aims to determine if this divergence extends to the proteins closely associated with these sequences and possibly even further into the …

The Effect Of Food Donations On The Micronutrient Intake Of Soup Kitchen Clients, Abigail Fudge

Honors Scholar Theses

For the past two years, this author has been the program director for a volunteer group called Food Recovery. Food Recovery collaborates with UConn Dining Services to collect unsold food items from UC Cafes on campus, and donates them to the Covenant Soup Kitchen in Willimantic, CT. The majority of the donations provided by Food Recovery are pre-packaged sandwiches and salads, that are made fresh daily by Dining Services staff and are sold in UC Cafes to the UConn community. The Covenant Soup Kitchen supports people experiencing food insecurity in Willimantic, by providing multiple hot meals throughout the week.

This …

Combinations Of Allelopathic Crop Extracts Reduce Digitaria Spp. And Setaria Faberi Seed Germination, Peter Apicella, Karl Guillard

Honors Scholar Theses

Allelopathic cover crops contain compounds that deter other types of plant seeds from germinating or inhibiting established plants’ growth. Sunflower (Helianthus annuus, SF), buckwheat (Fagopyrum esculentum Moench, BW), sorghum-sudangrass (Sorghum × drummondii [Nees ex. Steud.] Millsp. & Chase, SSG), and winter rye (Secale cereale) are all known allelopathic cover crops. However, there is little information about the use of these allelopathic cover crops used together and their combined impact on weed seed germination. Laboratory bioassays were conducted to determine the effect of the aforementioned cover crops alone and in combinations in reducing the …

Breaking Out From Tradition: Redesign Of Large Physiology Lecture Increases Engagement, Inclusion, And Student Outcomes, Jordyn Dickey, John Redden, Kristen Kimball

Honors Scholar Theses

The human digestive system is a diverse network of cells, tissues, and organs that is regulated by intrinsic (e.g. nervous and endocrine systems) and extrinsic factors (e.g. secretions, pH, and the microbiome). Given the volume of content and the dense physiology involved, this system is difficult for instructors to teach and equally challenging for students to understand. This is especially true in our two-semester Human Anatomy and Physiology course for pre-health students at the University of Connecticut. In the Spring 2017 semester, we developed and implemented an active learning based approach when teaching the histology and regulation of gastric secretions …

Characterizing Cultivable Bacteria From Trachymyrmex Septentrionalis Fungus Gardens, Hannah Beatty

Honors Scholar Theses

The relationship between the fungus-growing ant Trachymyrmex septentrionalis, its symbiotic cultivar fungus, and the transient and residential community of microorganisms is a diverse and complex symbiosis that has evolved over space and time. The fungus garden, comprised primarily of the cultivar fungus belonging to the family Leucocoprineae,provides an environment that hosts many bacteria, which may also play an important role in this symbiosis. Although it is known that Pseudonocardia bacteria defend the ant host against fungal pathogens, other species of bacteria that are present in these fungus gardens also likely contribute to this symbiosis. Previous studies of this …

How Does Body Size Affect Zooplankton Feeding In A Low Oxygen Environment?, Jacob Mikullitz

Honors Scholar Theses

Zooplankton grazers are essential to reducing the impact of algal blooms, especially as they become more frequent in a warming world. The size of these grazers is important as it determines how much algae they are able to consume. This study aims to investigate how low water oxygen content, another likely outcome of climate change, will impact competition between zooplankton of different size. When zooplankton of different sizes were tested against each other at different dissolved oxygen levels, it was found the larger species could feed more effectively at low oxygen. This suggests even in a future with reduced dissolved …

Effects Of Piggybac Transposable Element Derived 5 (Pgbd5) In Cortical Tissue, Benjamin Shao

Honors Scholar Theses

Many cancers are known to have genetic rearrangements, as these rearrangements develop abnormalities that can lead to certain types of cancer. Many of these genetic rearrangements can be caused by transposons, which are DNA sequences that can change its position and move to other positions within the genome. Transposons move to and integrate into a new location by the action of transposase enzymes, and recently genes that code for proteins similar to transposases and with transposase activity have been identified in the human and other vertebrate genomes. In this study, one of these genes, human piggyBac transposable element derived 5 …

The Ush2a Gene: An Analysis Of Ultrasonic Vocalizations In A Mouse Model Of Usher Syndrome Type 2, Kiana R. Akhundzadeh

Honors Scholar Theses

Usher syndrome type 2 is a complex autosomal recessive genetic disorder that is characterized by moderate to severe congenital sensorineural hearing loss, the onset of retinitis pigmentosa in the second decade of life, and in some cases, vestibular dysfunction. Mutations in the USH2A gene account for 85% of cases of type 2. The USH2A gene is responsible for encoding the protein usherin, which has an important role in the development and function of inner ear hair cells and retinal photoreceptors. Until recently, it has been believed that carriers of the USH2A mutation were phenotype free. However, recent data has suggested …

Identifying New Genes Involved In Centromere Establishment, Megan Boyer

Honors Scholar Theses

The centromere is a site on the chromosome that mediates accurate cell division by serving as a platform for kinetochore assembly, and microtubule attachment during cell division. Errors in the process of chromosome segregation can contribute to genetic irregularities, such as those seen in cancer and congenital defects. Our lab uses the ectopic centromere as a tool to discover what proteins may be involved in centromere establishment, defined as the deposition of CENP-A at the locus. We use the lacO/LacI system within Drosophila S2 cells that contain a CAL1-GFP- LacI transgene and an integrated lacO array to study the ectopic …

Is Concussion-Related Sleep Disturbance Present After Return To Play In College Athletes?, Alexander Gallaer

Honors Scholar Theses

As one of the most commonly experienced symptoms, the ramifications of sleep disruption as a result of concussion are potentially great, yet widely unexplored. Particularly troublesome is murky data regarding the length of sleep disruption following a concussion. By analyzing self-reported sleep data via the Pittsburgh Sleep Quality Index, this study seeks to investigate potential differences in sleep quality between injured college athletes 40 days after they have been cleared to play and matched controls. Data was analyzed using ANOVA analysis as well as Pearson correlation. No significant differences were found in sleep quality between groups, nor was there a …

Genotype-Specific Insertion Of Cytotoxic Genetic Elements Into Cancer Cells, Ryan Englander

University Scholar Projects

The new gene editing system CRISPR/Cas9, composed of a complex composed of a guide RNA and the Cas9 endonuclease, promises to revolutionize biological research and potentially allow clinicians to directly modify patient DNA in vivo. While its applications in the treatment of genetic diseases and in modifying immune cells for immunotherapy are currently being explored, CRISPR/Cas9’s potential utility as a modular system for targeting tumor-specific mutated sequences has not as of yet been explored. While CRISPR/Cas9 is specific enough to target small insertions and deletions or gross chromosomal rearrangements, it is not specific enough to reliably restrict editing to …

Investigation Of Bacteria From The Trachymyrmex Septentrionalis Fungus Garden For Potential Antibacterial Drug Leads, Brendan Stewart

Honors Scholar Theses

Bacterial and fungal strains are growing resistance to antibiotics and antifungal agents at an alarming rate. According to the Centers for Disease Control and Prevention, over two million people in the United States in 2016 were diagnosed with an infection resistant to antibiotics. As such, there has been increased interest in natural products as sources of novel compounds that are essential to the development of new drugs and treatment methods. Within the environment, there are various host-microbe symbioses, one of which is the Trachymyrmex septentrionalis leaf-cutter ant community. The microbes in symbioses like the T. septentrionalis community are hypothesized to …

Reconstitution Of Gabaergic Postsynapses In Host Cells, Karthik Kanamalla

Honors Scholar Theses

Type A GABA receptors (GABAARs) can be found embedded in postsynaptic membranes or in a variety of extrasynaptic locations. Receptors with synaptic function are recruited to the postsynapse by submembranous scaffolds composed of gephyrin and collybistin (CB). This study was aimed at assessing whether the ability to interact with the scaffold differentiates synaptic from non-synaptic receptors. Using HEK293 cells as an expression system, and indirect immunofluorescence (IF), co-localization of extrasynaptic receptors α1β3δ and α4β3δ with the CB-gephyrin scaffold was assessed and compared with that of the synaptic receptor α1β3γ2. Results indicated that both extrasynaptic receptors were able to colocalize with …

Investigation Of Bacteria From The Trachymyrmex Septentrionalis Fungus Garden For Potential Antibacterial Drug Leads, Brendan Stewart

University Scholar Projects

Bacterial and fungal strains are growing resistance to antibiotics and antifungal agents at an alarming rate. According to the Centers for Disease Control and Prevention, over two million people in the United States in 2016 were diagnosed with an infection resistant to antibiotics. As such, there has been increased interest in natural products as sources of novel compounds that are essential to the development of new drugs and treatment methods. Within the environment, there are various host-microbe symbioses, one of which is the Trachymyrmex septentrionalis leaf-cutter ant community. The microbes in symbioses like the T. septentrionalis community are hypothesized to …

Ecofeminism In The Speculative Fiction Of Ursula K. Le Guin, Octavia Butler, And Margaret Atwood, Cara Williams

Honors Scholar Theses

The aim of this article is to explore the speculative fiction works of three prominent, female speculative fiction writers: Ursula K. Le Guin, Margaret Atwood,and Octavia Butler through an ecofeminist lens. Ecofeminism, as first coined by Francois D'Eaubonne in 1974, is a philosophy that compares the oppression and abuse of women to that of the environment. This article notes how Le Guin, Atwood, and Butler portray women and the environment in post-apocalyptic science fiction. Specifically, this article looks at how these authors explore food acquisition and consumption in their various worlds. This article asks the question, how does our relationship …

Pertinacity Volume 4 Issue 1, Courteney Benjamin

Pertinacity

No abstract provided.

Wrack Lines Spring-Summer 2018, Judy Benson, Ann Baldelli, Bill Hanrahan, Ellen Johnson

Wrack Lines

From Sea to Table: Fish, Shellfish and sea vegetables from local waters