Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Entire DC Network
Targeted Germ Cell Knockdown Of Xlr3 In Mus Musculus, Caity Miller
Targeted Germ Cell Knockdown Of Xlr3 In Mus Musculus, Caity Miller
Honors Scholar Theses
The X chromosome is rather distinct from autosomes due to the unique regulatory and functional characteristics it exhibits. Xlr3 is just one gene in a superfamily of highly related, and homologous genes found on the X chromosome in mice. Xlr3 is part of a complex, imprinted locus, of which the function is not well understood, although its protein product can be found in testis and oocyte. However, it is theorized that the region may be implicated in the progression of meiosis, due to localization of XLR3. This study sought to characterize the function of Xlr3 through the use of a …
Examination Of The Chromatin Structure Of Xlr3b Using The Chromosome Conformation Capture Assay, Sarah Elise Conderino
Examination Of The Chromatin Structure Of Xlr3b Using The Chromosome Conformation Capture Assay, Sarah Elise Conderino
Honors Scholar Theses
Imprinted genes contain epigenetic modifications that influence expression patterns based on parent-of-origin. Recent studies have shown that imprinted genes contribute to numerous human diseases and disorders. Xlr3b, an imprinted gene on the X chromosome, has been implicated in social and behavioral deficits characteristic of disorders such as Turner syndrome and autism. The imprinting mechanism of this gene is still unknown, and this study analyzed the native chromatin structure of Xlr3b through the chromosome conformation capture assay to determine if there are any long-range interactions that regulate the expression of this gene. Brain tissue from a mouse model of Turner …
Macroh2a1 Regulation During The Cell Cycle Of Mouse Embryonic Stem Cells, Persis S. Thomas
Macroh2a1 Regulation During The Cell Cycle Of Mouse Embryonic Stem Cells, Persis S. Thomas
Honors Scholar Theses
MacroH2A is a core histone variant that plays an important role in the X-inactivation process during differentiation of embryonic stem cells. It has been shown that macroH2A changes in localization during the cell cycle of somatic cells. This study aims to determine how macroH2A changes during the cell cycle of embryonic stem cells. Male and female mouse embryonic stem cells were transfected with a GFP::macroH2A construct and the relationship between macroH2A and the cell cycle was determined using FACS. This study shows that macroH2A is altered during the cell cycle of embryonic stem cells as it is in somatic cells …