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- A First Survey of the Centipedes of Great Smoky Mountains National Park (1)
- BRAFV600E (1)
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- Chitosan (1)
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Articles 1 - 11 of 11
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Assessment Of The Triple Reuptake Inhibitor Diclofensine: Effort-Based Decision-Making In A Rodent Model Of Motivational Dysfunction, Sofia Papanikolaou
Assessment Of The Triple Reuptake Inhibitor Diclofensine: Effort-Based Decision-Making In A Rodent Model Of Motivational Dysfunction, Sofia Papanikolaou
Holster Scholar Projects
Serotonin-selective reuptake inhibitors (SSRIs) are the most commonly prescribed antidepressant medications. Despite their popularity, they remain relatively ineffective at treating effort-related motivational symptoms of depression such as fatigue and anergia. Increasing research on triple reuptake inhibitors (TRIs) that target three neurotransmitters—dopamine, serotonin, and norepinephrine—has suggested that TRIs could have efficacy in targeting motivational dysfunction due to their dopaminergic effects. Previous research has shown that the dopamine depleting agent tetrabenazine can reliably induce motivational deficits in rats, as evidenced by a shift towards low-effort behavior in effort-based choice tasks, and provide a validated approach to creating a model of motivational dysfunction. …
Creating An In-Vitro Co-Culture Model Of Human Megakaryocytic-Erythroid Progenitors And Endothelial Cells, Clerin John
Creating An In-Vitro Co-Culture Model Of Human Megakaryocytic-Erythroid Progenitors And Endothelial Cells, Clerin John
Holster Scholar Projects
Previous studies have suggested that endothelial cells promote red blood cell (RBC) production in human bone marrow, which has implications for improved treatments for anemia, cancer, and reproduction. However, investigation of this phenomenon is hindered by a dearth of studies that have successfully isolated primary endothelial cells from human bone marrow. Using flow cytometry, magnetic cell separation, and primary cell culture techniques we tested the hypothesis that adult bone marrow-derived endothelial cells can be isolated from previously frozen bone marrow samples by selecting for CD144+ expressing cells and culturing them in vitro. Magnetic cell separation utilizing the marker CD144 to …
Effect Of Chitosan On Plant Growth, Flowering, And Substrate Characteristics Of Potted Geranium (Pelargonium X Hortorum), Maggie Liu
Holster Scholar Projects
Modern industrial agricultural practices rely heavily on the use of fertilizers and pesticides to increase crop quality and quality which overtime gradually degrades the quality of cultivated soils and aquatic environments. The purpose of this project is to determine how chitosan polymers with various molecular weights affect the growth and flowering of Geranium plants as well as the water and nutrient retention capacity of the substrate. Three chitosans with different molecular weights (2 kDa, 50 kDa, and 970 Da) were experimented on the geraniums. Each chitosan treatment was applied through a spray system onto the experimental group plants after transplantation …
Enhancing Tomato Fruit Quality: Application Of Crispr/Dcas9-Mediated Methylation On The Pg Gene Promoter Region, Irena Komninakas
Enhancing Tomato Fruit Quality: Application Of Crispr/Dcas9-Mediated Methylation On The Pg Gene Promoter Region, Irena Komninakas
Holster Scholar Projects
Commercially produced tomatoes are typically harvested well before maturation in order to withstand domestic and international distribution. While this process ensures unspoiled goods, the nutritional value of the fruits have been curtailed as a result of premature harvesting. Previous research has found that the polygalacturonase gene (PG gene) is responsible for cell wall degradation of the tomato. In this study, CRISPR/dCas9 technology was employed to increase methylation levels in the promoter regions of the PG gene. This method is significant in that it epigenetically modifies the tomato genome using a technique that has the potential to develop non-genetically modified progeny …
Anatomical Characterization Of Descending Projections Of Lateral Hypothalamic Glutamatergic And Gabaergic Neurons, Jeremy Lesser
Anatomical Characterization Of Descending Projections Of Lateral Hypothalamic Glutamatergic And Gabaergic Neurons, Jeremy Lesser
Holster Scholar Projects
Important innate behaviors in mammals, such as sleep-wake regulation, arousal, eating, drinking, and stress, are associated with the lateral hypothalamic area (LHA). The LHA is a complex brain region consisting of broad populations of either excitatory glutamatergic neurons or inhibitory GABAergic neurons. Recent studies have revealed dense and heterogeneous fibers from both LHA populations projecting to neuromodulatory regions in the midbrain and brainstem, including the tuberomammillary nucleus (TMN), ventral tegmental area (VTA), dorsal raphe (DR), and locus coeruleus (LC). Viral tracing and immunohistochemistry were utilized to characterize the anatomy of LHAVGLUT2 and LHAVGAT projections to the aforementioned regions in an …
Examining Behavioral Defenses Of The Fungus-Growing Ant Trachymyrmex Septentrionalis Against Trichoderma-Derived Metabolites, Darren Lee
Holster Scholar Projects
Fungus-growing ants defend their fungal mutualist against pathogens through a number of chemical and behavioral mechanisms. Work by Dr. Jonathan Klassen’s lab has found that Trachymyrmex septentrionalis ants maintain their fungal symbionts’ health through physical removal (“weeding”) upon infection with pathogenic fungi in the genus Trichoderma. Ants also exhibited weeding behavior when the fungus was inoculated with peptaibols, a class of secondary metabolites produced by Trichoderma, suggesting that these molecules act as chemical cues that induce ant weeding. However, the mechanisms by which weeding behaviors are communicated between the ants and their fungal symbiont are unknown. Behavioral experiments demonstrated that …
Analysis Of Tri-Lineage Collagen Reporter Mice Growth Plates Through Light Sheet Microscopy, Olivia Ferrigno
Analysis Of Tri-Lineage Collagen Reporter Mice Growth Plates Through Light Sheet Microscopy, Olivia Ferrigno
Holster Scholar Projects
The growth plate is an active area of cartilage in the long bones of children. Its main function is to enable limb lengthening, but is a relatively weak structure that is prone to fractures and injuries. These injuries can have severe long term ramifications, as they can create premature bone fusion, also known as a bony bridge, that causes stunted or crooked growth. Dr. Liisa Kuhn’s Lab has developed an accurate, reproducible animal model that mimics this same growth plate behavior and can be used to further analyze the growth plate structure, healing process, and new treatments. This animal model …
Determining The Primary Dna Substrates Of Shld2'S Ob-Fold Domains, Hari Patchigolla
Determining The Primary Dna Substrates Of Shld2'S Ob-Fold Domains, Hari Patchigolla
Holster Scholar Projects
Failure to repair DNA double-stranded breaks leads to cell death. Radiation therapy is commonly used to kill cancer cells by inducing these breaks. However resistance to radiation therapy, due to a hyperactive DNA double-stranded break repair pathway, is a common occurrence that makes cancer patients more prone to relapse. The Shieldin complex is shown to promote DNA-double stranded break repair by binding to DNA at sites of damage. Thus, the objective of this project is to understand the affinity and type of DNA that Shieldin binds to, through gel-shift assays, for the eventual creation of an inhibitor for this protein …
Identification Of Secretion Factors That Restructure Neural Tissue In A Model Of Low Grade Glioma, Jackson Ayers
Identification Of Secretion Factors That Restructure Neural Tissue In A Model Of Low Grade Glioma, Jackson Ayers
Holster Scholar Projects
Pediatric Low-grade Gliomas account for up to ⅔ of all pediatric tumors. Despite their low grade classification, they have the ability to mutate farther to a grade 3 or 4 neurological tumor, exponentially decreasing treatment and survival rates. Identifying the specific secretion factors associated with these tumors is extremely beneficial towards possible treatment options. CRISPR/Cas9 constructs were implemented to knock out GDF-15 and CCl-3 secretion factors in the presence of a BRAFV600E mutation before concentrated DNA plasmids were injected and integrated into the brains of mice embryos through In Utero Electroporation (IUE). The brains were subsequently stained through an immunohistochemistry …
A First Survey Of The Centipedes Of Great Smoky Mountains National Park, Joseph Desisto
A First Survey Of The Centipedes Of Great Smoky Mountains National Park, Joseph Desisto
Holster Scholar Projects
No abstract provided.
Dietary Analysis And Epigenetic Comparisons Of Drosphila Melanogaster Through Multiple Generations, Xu (Kevin) F. Zheng
Dietary Analysis And Epigenetic Comparisons Of Drosphila Melanogaster Through Multiple Generations, Xu (Kevin) F. Zheng
Holster Scholar Projects
Nutrition and the lack thereof has been a demanding issue in both the undeveloped and developed country. The unbalance diet between healthy and unhealthy diet has led to many of the problems presented in society such as obesity, heart disorders, and loss of longevity. It is now known that calorie may not provide the most accurate information in terms of the lifestyle and the well-being of humans. Rather the composition of the calorie is what becomes integral in people's understanding of the effect of food physiologically. With this knowledge, we seek to use a Drosophila melanogaster as a model organism …