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The Glycine And Proline Reductase Systems: An Evolutionary Perspective And Presence In Enterobacteriaceae, Joshua Witt
The Glycine And Proline Reductase Systems: An Evolutionary Perspective And Presence In Enterobacteriaceae, Joshua Witt
HIM 1990-2015
The Glycine and Proline Reduction systems are two of the best characterized selenoenzymes in bacteria and have been found to occur in a wide variety of clostridia [1-5]. These enzymes are utilized to reduce glycine or D-proline to obtain energy via substrate level phosporylation or membrane gradients, respectively [6, 7]. This includes the pathogens C. difficile and C. botulinum [5, 8]. Strains of C. difficile are activate toxigenic pathways whenever either of these pathways is active within the cell [5, 8]. Though evolutionary studies have been conducted on ammonia producing bacteria [9] none has been done to directly characterize these …
The Effects Of Growth Hormone And Thyroxine Treatment On The Insulin Signaling Of Female Ames Dwarf Mouse Skeletal Muscle Tissue, Andrew Do
HIM 1990-2015
Ames dwarf (df/df) mice are deficient in anterior pituitary hormones: growth hormone (GH), thyroid stimulating hormone (TSH), and prolactin (PRL) due to a spontaneous, homozygous mutation of prop1[superscript df] gene. These dwarf mice exhibit characteristics such as delayed growth and development coupled with delayed aging, increased lifespan, overall increased insulin sensitivity, as well as resistance to certain diseases and cancers. The mutant mice possess low blood glucose, low serum insulin, and lower body temperature. Their enhanced longevity (about 40-60% longer lifespan than normal mice) is associated with their GH deficiency and disruption in the somatotropic axis (GH/IGF-1 hormonal pathway) as …
Manipulating Aktivated Metabolism Via Mtorc1, Ivan Von Hack Prestinary
Manipulating Aktivated Metabolism Via Mtorc1, Ivan Von Hack Prestinary
HIM 1990-2015
Although poorly understood, normal cells and cancerous cells of the same type exhibit different patterns of nutrient consumption, processing and utility of metabolic substrates. Differences in substrate uptake, preference, and alternately emphasized metabolic pathways offer opportunities for selective targeting of cancer versus stroma. This may be accomplished by using a sequential approach of nutrient deprivation and pharmaceutical perturbation of metabolic pathways to inhibit cellular proliferation. The purpose of this study was to investigate the effects of restricting glucose and glutamine concentrations, in vitro, to levels that resemble a potential human fasting state. The mammalian target of rapamycin (mTOR), a mediator …
As The Seas Rise: How Coastal Plants And Animals Will Be Affected By Sea Level Rise, Linda Walters, Sydney Katz
As The Seas Rise: How Coastal Plants And Animals Will Be Affected By Sea Level Rise, Linda Walters, Sydney Katz
CEELAB Children's Books
Lottie the Loggerhead Sea Turtle, Marshall and Marsha Marshgrass, and Ollie the Eastern Oyster are here to tell their stories. You'll learn about each of them and how their futures are threatened by sea level rise. So read this book, and get to know your coastline!
Juvenile Ornamentation: Its Evolution, Genetic Basis, And Variation Across Habitats, Angela Tringali
Juvenile Ornamentation: Its Evolution, Genetic Basis, And Variation Across Habitats, Angela Tringali
Electronic Theses and Dissertations
Ornamental traits are considered honest advertisements of fitness, and their evolution is usually explained in terms of sexual selection. This explanation remains unsatisfactory in some instances, for example, juvenile birds whose plumage is molted prior to adulthood and breeding. I first evaluate whether juvenile plumage reflectance signals dominance status in the Federally Threatened Florida scrub-jay (Aphelocoma coerulescens) using a combination of observational and experimental methods. Then I estimate the heritability, non-genetic maternal and environmental effects, and strength of selection on juvenile plumage reflectance using archived feather samples and a pedigree constructed from historical nest records. Finally, I compare plumage reflectance …
Target Validation For Neurofibromatosis Type 2 Therapeutics., Alejandra Guinart
Target Validation For Neurofibromatosis Type 2 Therapeutics., Alejandra Guinart
Electronic Theses and Dissertations
Neurofibromatosis type 2 (NF2) is a benign tumor disease of the nervous system. Development of bilateral vestibular schwannomas is characteristic of NF2; however patients frequently present schwannomas on other nerves, as well as meningiomas and ependymomas. Currently, there are no drug therapies for NF2. There is an urgent need for development of NF2 therapeutics and this dissertation presents two independent potential therapeutic targets. The disease is caused by mutations in the NF2 gene that encodes a tumor suppressor called merlin. Loss of merlin function is associated with increased activity of Rac and p21-activated kinases (PAK) and deregulation of cytoskeletal organization. …
Characterization Of Innate Immunity In The Female Reproductive Tract For The Prevention Of Hiv Acquisition, Colleen Eade
Characterization Of Innate Immunity In The Female Reproductive Tract For The Prevention Of Hiv Acquisition, Colleen Eade
Electronic Theses and Dissertations
Human immunodeficiency virus (HIV) infects 30 million people worldwide. In sub-Saharan Africa, the region most affected by HIV, women comprise 60% of the infected population. Heterosexual transmission is a major mode of viral acquisition, mandating further research of the process and prevention of HIV acquisition via the female reproductive tract (FRT). The FRT is a dynamic environment, protected by host immune mechanisms and commensal microbes. The disruption of either of these elements can increase susceptibility to HIV. Accordingly, one common risk factor for HIV acquisition is the microbial shift condition known as bacterial vaginosis (BV), which is characterized by the …
Molecular Regulators Of Post-Golgi Vldl Transport Vesicle (Pg-Vtv) Biogenesis, Aladdin Riad
Molecular Regulators Of Post-Golgi Vldl Transport Vesicle (Pg-Vtv) Biogenesis, Aladdin Riad
Electronic Theses and Dissertations
Amongst its numerous functions, the liver is responsible for the synthesis and secretion of very low-density lipoprotein (VLDL). VLDL particles play the important role of facilitating the transport of lipids within the aqueous environment of the plasma; yet high plasma concentrations of these particles result in the pathogenesis of atherosclerosis, while low VLDL secretion from the liver results in hepatic steatosis. VLDL synthesis in the hepatocyte is completed in the Golgi apparatus, which serves as the final site of VLDL maturation prior to its secretion to the bloodstream. The mechanism by which VLDL’s targeted transport to the plasma membrane is …
Immuno-Pcr Detection Of Lyme Borreliosis, Micah Halpern
Immuno-Pcr Detection Of Lyme Borreliosis, Micah Halpern
Electronic Theses and Dissertations
Lyme borreliosis, more commonly referred to as Lyme disease, is the fastest growing zoonotic disease in North America with approximately 30,000 confirmed cases and 300,000 estimated infections per year. In nature, the causative agent of Lyme disease, the bacterium Borrelia burgdorferi, cycles between Ixodes sp. ticks and small mammals. Humans become infected with Lyme disease after being bitten by an infected tick. The primary indicator of a Borrelia burgdorferi infection is a bull’s eye rash typically followed by flu-like symptoms with treatment consisting of a 2-4 week course of antibiotics. If not treated, later stages of the disease can result …
Screening Of Quantum Dots For Toxicity On The Growth And Viability Of Escherichia Coli, Jeremy Tharkur
Screening Of Quantum Dots For Toxicity On The Growth And Viability Of Escherichia Coli, Jeremy Tharkur
Electronic Theses and Dissertations
Heavy metal (HM) containing quantum dots (Qdots) are increasingly used in commercial products due to their unique electronic, optoelectronic, optical and magnetic properties. Once disposed to the landfill, environmental weathering is likely to compromise HM Qdot integrity, leading to release of heavy metal ions. To minimize any negative environmental impact of HM Qdots, there is an increasing demand for developing HM free or environmentally-friendly surface modified HM Qdot alternatives. In this study, synthesis of HM free ZnS:Mn/ZnS and surface modified HM CdS:Mn/ZnS Qdots (using N-acetylcysteine, NAC, and Dihydrolipoic acid, DHLA) and their potential toxicity assessment using E. coli as a …
Cerium Oxide Nanoparticles Sensitize Pancreatic Cancer Cells To Radiation By Promoting Acidic Ph, Ros, And Jnk Dependent Apoptosis, Melissa Wason
Cerium Oxide Nanoparticles Sensitize Pancreatic Cancer Cells To Radiation By Promoting Acidic Ph, Ros, And Jnk Dependent Apoptosis, Melissa Wason
Electronic Theses and Dissertations
Side effects of radiation therapy (RT) remain the most challenging issue for pancreatic cancer treatment. In this report we determined whether and how cerium oxide nanoparticles (CONPs) sensitize pancreatic cancer cells to RT. CONP pretreatment enhanced radiation-induced reactive oxygen species (ROS) production preferentially in acidic cell-free solutions as well as acidic human pancreatic cancer cells. In acidic environments, CONPs favor the scavenging of superoxide radical over the hydroxyl peroxide resulting in accumulation of the latter whereas in neutral pH CONPs scavenge both. CONP treatment prior to RT markedly potentiated the cancer cell apoptosis both in culture and in tumors and …
Wetland Diversity In A Disturbance-Maintained Landscape: Effects Of Fire And A Fire Surrogate On Aquatic Amphibian Survival And Species Depauperateness., Joyce Klaus
Electronic Theses and Dissertations
Disturbance is one of the central concepts explaining how diversity arises and is perpetuated in ecological time. A good model system for testing hypotheses related to disturbance is the longleaf pine ecosystem in the southeastern U.S. because in this ecosystem frequent, low-severity fires acts as a disturbance that maintains a unique vegetation structure and high species richness. Vegetation structure influences animal distributions; in fire-dependent ecosystems many animals rely on open-structured, fire-maintained vegetation but shrubs and trees encroach into fire-dependent ecosystems where fire has been excluded. Prescribed burning and mechanical removal are commonly used as restoration tools to control encroachment. To …
Testing For Indirect Benefits Of Polyandry In The Florida Green Turtle, Christopher Long
Testing For Indirect Benefits Of Polyandry In The Florida Green Turtle, Christopher Long
Electronic Theses and Dissertations
Behavioral studies in the green turtle (Chelonia mydas) have indicated that promiscuous mating is commonplace. Though it has been shown that there is much variation in the rate of polyandry (females mating with multiple males), the drivers behind polyandry in this species are unknown. It has been speculated, but never demonstrated, that indirect benefits (fitness benefits resulting from offspring genetic diversity) play a role. However, previous tests of this hypothesis have limited scope of inference due to lack of environmental control. In this thesis, I attempted to study the indirect benefits of polyandry in Archie Carr National Wildlife Refuge (ACNWR) …
Florida Local Government Conservation Planning: Variability, Drivers, And Policy Implications, Pamela Pannozzo
Florida Local Government Conservation Planning: Variability, Drivers, And Policy Implications, Pamela Pannozzo
Electronic Theses and Dissertations
This study examined the quality of Florida county government conservation planning. To assess conservation planning quality, a theoretical model of conservation planning as prescribed by the conservation science literature was first developed. A plan evaluation coding protocol was applied to local comprehensive plan Conservation Elements to determine the extent to which county-level conservation planning met the theoretical model. A high degree of variability in conservation planning quality was found. Highest quality conservation planning occurred in the Gulf coast counties of southwest Florida. Lowest conservation planning quality occurred in the Florida Panhandle counties. The quality of conservation planning of coastal counties …
Do Changes In Muscle Architecture Effect Post- Activation Potentiation, Danielle Reardon
Do Changes In Muscle Architecture Effect Post- Activation Potentiation, Danielle Reardon
Electronic Theses and Dissertations
Purpose: To examine the effect of three muscle potentiation protocols on changes in muscle architecture and the subsequent effect on jump power performance. Methods: Maximal (1RM) squat strength (Mean SD=178.3 ± 36.6kg), vertical jump power, and muscle architecture were obtained in 12 resistance trained men (25.2±3.6y; 90.67±12.7kg). Participants randomly completed three squatting protocols at 75% (3 x 10 reps), 90% (3 x 3 reps) or 100% (1 x 1) of their 1RM, or no workout (CON), with each protocol being separated by one week. During each testing session ultrasound and vertical jump testing were assessed at baseline (BL), 8min post …
Relating Climate Change To The Nesting Phenology And Nest Environment Of Marine Turtles, Monette Schwoerer
Relating Climate Change To The Nesting Phenology And Nest Environment Of Marine Turtles, Monette Schwoerer
Electronic Theses and Dissertations
Ectotherms (including marine turtles) being especially sensitive to climate, are at risk to the accelerated rate of human-driven climate change. This study addresses two concerns associated with marine turtles and climate change – the relationship between the timing of marine turtle nesting and sea surface temperature; and the concern over the feminization of marine turtle populations due to rising sand temperatures. Previous studies of loggerhead sea turtles (Caretta caretta) and green sea turtles (Chelonia mydas) have documented the relationship between sea surface temperatures and nesting phenology. Earlier nesting behaviors in both species have been associated with warmer sea surface temperatures. …
Assessing The Effectiveness Of Living Shoreline Restoration And Quantifying Wave Attenuation In Mosquito Lagoon, Florida, Jennifer Manis
Assessing The Effectiveness Of Living Shoreline Restoration And Quantifying Wave Attenuation In Mosquito Lagoon, Florida, Jennifer Manis
Electronic Theses and Dissertations
Coastal counties make up only 17% of the land area in the continental United States, yet 53% of the nation’s population resides in these locations. With sea level rise, erosion, and human disturbances all effecting coastal areas, researchers are working to find strategies to protect and stabilize current and future shorelines. In order to maintain shoreline stability while maintaining intertidal habitat, multipurpose living shorelines have been developed to mimic natural shoreline assemblages while preventing erosion. This project determined the effectiveness of a living shoreline stabilization containing Crassostrea virginica (eastern oyster) and Spartina alterniflora (smooth cordgrass) in the field and through …
Sex Ratios Of Juvenile Green Turtles (Chelonia Mydas) In Three Developmental Habitats Along The Coast Of Florida, Cheryl Sanchez
Sex Ratios Of Juvenile Green Turtles (Chelonia Mydas) In Three Developmental Habitats Along The Coast Of Florida, Cheryl Sanchez
Electronic Theses and Dissertations
The concept of temperature dependent sex determination (TSD) has been somewhat of an evolutionary enigma for many decades and has had increased attention with the growing predictions of a changing climate, particularly in species that are already threatened or endangered. TSD taxa of concern include marine turtles, which go through various life stages covering a range of regions. This, in turn, creates difficulties in addressing basic demographic questions. Secondary sex ratios (from life stages post-hatchling) were investigated by capturing juvenile green turtles (Chelonia mydas), 22.6-60.9 cm in straight carapace length (SCL), from three developmental areas along the east coast of …
Population Dynamics And Environmental Factors Influencing Herbs In Intact And Degraded Florida Rosemary Scrub, Elizabeth Stephens
Population Dynamics And Environmental Factors Influencing Herbs In Intact And Degraded Florida Rosemary Scrub, Elizabeth Stephens
Electronic Theses and Dissertations
Species have complex and contextual relationships with their environment; both the relative contributions of life-history stages to population growth and the effect of environmental factors on each stage can be different among co-existing species. Timing and extent of reproduction, survival, and mortality determine population growth, species distributions, and assemblage patterns. I evaluate the role of habitat (intact, degraded) and microsite (shrub, leaf litter, bare sand) on population dynamics of Florida scrub herbs. Isolated overgrown shrubs and extensive bare sand areas in degraded scrub were expected to decrease seed predation, reduce competition of herbs with shrubs, and provide larger habitat for …
Identification Of Epithelial Stromal Interaction 1 And Epidermal Growth Factor Receptor As Novel Kruppel-Like Factor 8 Targets In Promoting Breast Cancer Progression, Tianshu Li
Electronic Theses and Dissertations
Breast cancer is the major cause of cancer death among women worldwide. Understanding the mechanisms underlying breast cancer progression remains urgent for developing effective treatment strategies to eliminate breast cancer mortality. Our recent studies have demonstrated that Krüppel-like transcriptional factor 8 (KLF8) plays a critical role for breast cancer progression. Other studies have shown that Epithelial stromal interaction 1 (EPSTI1), a recently identified stromal fibroblast-induced gene in non-invasive breast cancer cells and epidermal growth factor receptor (EGFR) are highly overexpressed in aggressively invasive breast carcinomas including triple negative breast cancers. In this thesis project, we demonstrate high co-overexpression of KLF8 …
Is Breakdown Of Fatty Acid Peroxides Involved In The Induction Of Apolipoprotein A1?, Rajat Gupta
Is Breakdown Of Fatty Acid Peroxides Involved In The Induction Of Apolipoprotein A1?, Rajat Gupta
Electronic Theses and Dissertations
Over the past few years the number of deaths caused due to cardiovascular diseases has been increasing and is of major concern. In the United States, 75% of cardiovascular-related deaths have been attributed to atherosclerosis. Western diets containing large quantities of peroxidized lipids are considered atherogenic. Heated oil in the form of fried food brings high levels of peroxidized fat and its decomposition products in the diet. Peroxidized lipids are known to increase the susceptibility of serum lipoproteins to undergo oxidation, thereby contributing to the progression of atherosclerosis. The intestinal cells are responsible for the absorption of dietary fatty acid …
Characterization Of Motility Alterations Caused By The Impairment Of Dynein/Dynactin Motor Protein Complex, Swaran Nandini
Characterization Of Motility Alterations Caused By The Impairment Of Dynein/Dynactin Motor Protein Complex, Swaran Nandini
Electronic Theses and Dissertations
Transport of intracellular cargo is an important and dynamic process required for cell maintenance and survival. Dynein is the motor protein that carries organelles and vesicles from the cell periphery to the cell center along the microtubule network. Dynactin is a protein that activates dynein for this transport process. Together, dynein and dynactin forms a motor protein complex that is essential for transport processes in all the vertebrate cells. Using fluorescent microscope based live cell imaging techniques and kymograph analyses, I studied dynein/dynactin disruptions on the intracellular transport in two different cell systems. In one set of experiments, effects of …
Cholera Toxin Activates The Unfolded Protein Response Through An Adenylate Cyclase-Independent Mechanism, Neyda Vanbennekom
Cholera Toxin Activates The Unfolded Protein Response Through An Adenylate Cyclase-Independent Mechanism, Neyda Vanbennekom
Electronic Theses and Dissertations
Cholera toxin (CT) is a bacterial protein toxin responsible for the gastrointestinal disease known as cholera. CT stimulates its own entry into intestinal cells after binding to cell surface receptors. Once internalized, CT is delivered via vesicle-mediated transport to the endoplasmic reticulum (ER), where the CTA1 subunit dissociates from the rest of the toxin and is exported (or translocated) into the cytosol. CTA1 translocates from the ER lumen into the host cytosol by exploiting a host quality control mechanism called ER-associated degradation (ERAD) that facilitates the translocation of misfolded proteins into the cytosol for degradation. Cytosolic CTA1, however, escapes this …
The Effects Of 6-Weeks Of Resistance Training On The Neuromuscular Fatigue Threshold In Older Adults, Nadia Emerson
The Effects Of 6-Weeks Of Resistance Training On The Neuromuscular Fatigue Threshold In Older Adults, Nadia Emerson
Electronic Theses and Dissertations
Age-related deficits in muscle mass, strength, and function place an increased burden of work on existing skeletal muscle and may lead to early onset of neuromuscular fatigue (NMF) during activities of daily living. Resistance exercise (RE) is the proven method for improving neuromuscular function in healthy older adults. PURPOSE: To investigate the effects of 6 weeks of RE on the NMF threshold as well as strength and functional performance in older adults. METHODS: Twenty-four older adults were randomly assigned to 6 weeks of RE (EXE; n = 12; age 72 ± 6.3 y; BMI 28.4 kg/m2 ) or control (CONT; …
Modeling Survival Of Immature Loggerheads (Caretta Caretta) And Green Turtles (Chelonia Mydas) From 10 Years Of Mark-Recapture Data At The Florida Power And Light St. Lucie Plant, Andrew Sterner
Electronic Theses and Dissertations
Loggerheads (Caretta caretta) are listed as Threatened and green turtles (Chelonia mydas) are listed as Endangered under the United States Endangered Species Act. While green turtle nest production in Florida has increased markedly in recent years, loggerhead nest production has followed a more tenuous path. Reasons for these differences are unknown. Limited demographic information is available for these species of conservation concern. I used Barker models, which incorporated mark-recapture, live-resight and dead recovery data, implemented in Program MARK. These models were used to estimate apparent survival for immature loggerhead (
Disturbance-Based Management And Plant Species Change In Massachusetts Sandplain Heathlands Over The Past Two Decades, Kirsten Martin
Disturbance-Based Management And Plant Species Change In Massachusetts Sandplain Heathlands Over The Past Two Decades, Kirsten Martin
Electronic Theses and Dissertations
Massachusetts sandplain heathlands are habitats of conservation concern, harboring many rare plant species and providing habitat for animals that depend on openlands. These heathlands are threatened by human development, shrub encroachment in the absence of disturbance, and potentially increasing soil nutrient levels. Sandplain heathlands are managed with prescribed fire, in order to maintain their open structure and maintain species diversity. In order to assess how past management was correlated with species change, I used a data set that spanned twenty years from three different heathlands in Massachusetts. I looked for correlations between management and species change. Correlations between species change …
The Contribution Of Visceral Fat To Positive Insulin Signaling In Ames Dwarf Mice, Vinal Menon
The Contribution Of Visceral Fat To Positive Insulin Signaling In Ames Dwarf Mice, Vinal Menon
Electronic Theses and Dissertations
Ames dwarf (df/df) mice are homozygous for a spontaneous mutation in the prop1 gene due to which there is no development of anterior pituitary cells – somatotrophs, lactotrophs and thyrotrophs, leading to a deficiency of growth hormone (GH), prolactin (PRL) and thyrotropin (TSH). They tend to become obese as they age, but still live longer and healthier lives compared to their wild-type littermates, being very insulin sensitive, showing no signs of diabetes and cancer. These mutant mice also have high circulating levels of anti-inflammatory and antidiabetic adiponectin. Plasma levels of this adipokine usually decrease with an increase in accumulation of …
Identification Of Potential Lead Antimalarial Compounds From Marine Microbial Extracts, Abigail Carbonell
Identification Of Potential Lead Antimalarial Compounds From Marine Microbial Extracts, Abigail Carbonell
HIM 1990-2015
Malaria, caused by the parasite Plasmodium falciparum, has a long history as a global health threat. The vector-borne disease causes millions of deaths yearly, especially in developing countries with tropical climates that facilitate transmission. Compounding the problem is the emergence of drug-resistant strains due to overuse of outdated treatments. New compounds with antiplasmodial activity are needed to be developed as effective drugs against malaria. The hypothesis for this project is that marine microorganisms have a high likelihood of yielding novel antiplasmodial chemotypes because of their high diversity, which has not yet been explored for antimalarial development. In this project, microbes …
Development Of An Alkaline Phosphatase Reporter System For Use In The Lyme Disease Spirochete Borrelia Burgdorferi, Selina Sutchu
Development Of An Alkaline Phosphatase Reporter System For Use In The Lyme Disease Spirochete Borrelia Burgdorferi, Selina Sutchu
HIM 1990-2015
The use of the periplasmic alkaline phosphatase (PhoA) reporter protein from E. coli has been critical for definition of the topology of transmembrane proteins of multiple bacterial species. This report demonstrates development of a PhoA reporter system in B. burgdorferi. Codon usage of the E. coli phoA in B. burgdorferi was analyzed and an optimized version of the gene was obtained. In order to assess the differential activity of the reporter system, two optimized PhoA-fusion construct using B. burgdorferi proteins were engineered: one using the periplasmic protein OppAIV and one using the cytoplasmic protein PncA. The activity of PhoA requires …
Expression Of An Epitope Tagged Tarp Effector In Chlamydia Trachomatis, Brenda Nguyen
Expression Of An Epitope Tagged Tarp Effector In Chlamydia Trachomatis, Brenda Nguyen
HIM 1990-2015
Previous studies performed on Chlamydia trachomatis have demonstrated how these obligate intracellular microbes invade host cells through the utilization of secreted effector proteins. One secreted effector called Tarp (translocated actin recruiting protein) is implicated in cytoskeleton rearrangements that promote bacterial entry into the host cell. The focus of our study is to create a plasmid that carries the tarP gene that when transcribed and translated from within Chlamydia trachomatis will generate a c-Myc epitope tagged Tarp. The tag will be used in future studies to track the progression of the protein through the infectious process and will allow us to …