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The Feasibility Of Detecting Poliovirus I In Water By Radioimmunoassay, Sheril K. Charba
The Feasibility Of Detecting Poliovirus I In Water By Radioimmunoassay, Sheril K. Charba
Retrospective Theses and Dissertations
A microtiter, solid-phase, indirect radioimmunoassay (RIA) has been developed and evaluated as a method for detecting poliovirus in water samples. Antiserum to poliovirus Type I, LSc2ab, was prepared in rabbits, and high titer, high avidity antiserum to rabbit globulins were radio- actively labeled with 125I by a modification of the Bolton-Hunter and chloramine T methods to increasing specific activities. The immunoreactivity of the labeled antibodies was assessed. After the preparation and standardization of all components of the assay. The optimum assay conditions were determined. These conditions included the method of coating the microtiter wells with antigen, the time and temperature …